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Kinetic and Thermodynamic Analysis of AChE Inhibition of Solvent Extract Fractions from Inonotus obliquus  

Kim, Hak-Kyu (Korea Accelerator and Plasma Research Assoiation)
Hur, Won (Department of Bioengineering and Technology, Kangwon National University)
Hong, Eok Kee (Department of Bioengineering and Technology, Kangwon National University)
Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
Publication Information
Food Engineering Progress / v.15, no.4, 2011 , pp. 289-296 More about this Journal
Abstract
Twenty four fractions by solvent extraction and/or acid precipitation from fruit body and culture broth of Inonotus obliquus were prepared, and their inhibitory effect against acetylcholinesterase (AChE) was investigated. Among these fractions, acid (1 M HCl) precipitates from cell-free culture broth and fruit body exhibited the highest inhibitory effect on AChE in vitro. Acid precipitates inhibited AChE activity in a concentration-dependant manner and $IC_{50}$ values of both acid precipitates were 0.53 mg/mL. The inhibition pattern was general non-competitive inhibition. The energetic parameters were also determined by dual substrate/temperature design. Both acid precipitates increased the values of Ea, ${\Delta}H,/;{\Delta}G$ and ${\Delta}H^{\ast}$ decreasing the value of ${\Delta}S$ for AChE. The results implied that the acid precipitates from I. obliquus increased the thermodynamic barrier, leading to the breakdown of ES complex and the formation of products as inhibitory mechanism.
Keywords
Inonotus obliquus; solvent extract; acid precipitate; acetylcholinesterase; inhibition kinetics; thermodynamic analysis;
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