• Korean Journal of Food Science and Technology
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• v.51 no.6
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• pp.576-583
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• 2019

The effect of aeration process in causing changes in the prosapogenin content and the antioxidant activity of red ginseng powder extracts was investigated. With respect to the color change of the extracts, the L-value and b-value decreased significantly with the lapse of extraction time both with and without the aeration process. The a-value increased with the lapse of the extraction time in the non-aeration process but decreased in the aeration process. This result suggests that when the aeration process was performed, the lightness, yellowness, and redness decreased with the lapse of the extraction time, resulting in a darker color. The total polyphenolic and total flavonoid contents were the highest at 0.84 and 0.96 mg Gallic Acid Equivalent (GAE)/mL, 21.77 and 21.93 mg GAE/mL at 24 h and 36 h, respectively for the aerated red ginseng powder extracts. The DPPH, ABTS, H₂O₂ scavenging activity, and reducing power were measured to confirm the antioxidant effects of red ginseng powder extracts after the aeration process. Thus, the antioxidant activity was increased in the aerated red ginseng powder extracts. In addition, when comparing the contents of Rb1, Rg1, and Rg3, the content of Rg3 was significantly different, and it was confirmed that a large amount was produced in the aerated red ginseng extracts. These results indicate that the red ginseng extracts subjected to the aeration process are superior than the ones processed by the non-aeration process.

• Journal of Genetic Medicine
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• v.7 no.1
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• pp.53-58
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• 2010

Purpose: To find the most effective method for extraction of cell-free DNA (cf-DNA) from maternal plasma, we compared a blood DNA extraction system (blood kit) and a viral DNA extraction system (viral kit) for non-invasive first-trimester fetal gender determination. Materials and Methods: A prospective cohort study was conducted with maternal plasma collected from 44 women in the first-trimester of pregnancy. The cf-DNA was extracted from maternal plasma using a blood kit and a viral kit. Quantitative fluorescent-polymerase chain reaction (QF-PCR) was used to detect the SRY gene and AMEL gene. The diagnostic accuracy of the QF-PCR results was determined based on comparison with the final delivery records. Results: A total of 44 women were tested, but the final delivery record was only obtained in 36 cases which included 16 male-bearing and 20 female-bearing pregnancies. For the blood kit and viral kit, the diagnostic accuracies for fetal gender determination were 63.9% (23/36) and 97.2% (35/36), respectively. Conclusion: In non-invasive first-trimester fetal gender determination by QF-PCR, using a viral kit for extraction of cf-DNA may result in a higher diagnostic accuracy.

• Journal of Periodontal and Implant Science
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• v.39 no.3
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• pp.367-373
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• 2009

Purpose: Following tooth extraction caused by severe periodontitis, alveolar ridge dimension lose their original volume. To reduce the alveolar ridge dimension, the ridge preservation technique has been introduced and tested in many clinical studies with membrane alone or membrane plus graft, achieving reduced ridge loss compared to extraction only. The aim of the present clinical study was to compare the post-extraction dimensional changes in the membrane exposure group to non-exposure group during healing period following ridge preservation technique. Methods: Ridge preservation was performed in 44 extraction sites. After extraction, deproteinized bovine bone mineral coated with synthetic oligopeptide (Ossgen-$X15^{(R)}$) or deproteinized bovine bone mineral (Bio-$Oss^{(R)}$) was implanted into the socket. A collagen membrane (Bio-$Gide^{(R)}$) was trimmed to cover the socket completely and applied to the entrance of the socket. Four clinical parameters were compared between baseline and 6 months. Results: During healing period, membrane exposure was observed at 19 sites. At the re-entry, hard newly formed tissue were observed at the ridge preservation site. The grafted socket sites were well preserved in their volume dimension. In both groups, horizontal ridge width was reduced and vertical height was increased. There were not statistically significant differences in horizontal (-1.32 mm vs -1.00 mm) and vertical ridge change (2.24 mm vs 2.37 mm at buccal crest, 1.36 mm vs. 1.53 mm at lingual crest) between two groups. Conclusions: The ridge preservation approach after tooth extraction effectively prevented resorption of hard tissue ridge in spite of membrane exposure during healing period.

• Journal of Korea Soil Environment Society
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• v.4 no.1
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• pp.3-11
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• 1999

The effects of major cations in soils, soil : extractant ratio, and EDTA : lead stoichiometric ratio on the extraction efficiency of lead using EDTA were studied for 4 different actual lead-contaminated soils and one artificially lead-contaminated soil. Extraction of lead from the lead-contaminated soil was not affected by a soil : extractant ratio as low as 1 : 3 but instead was dependent on the quantity of EDTA present. Results of the experiments showed that the extraction efficiency for each soil was different, but if sufficiently large amount of EDTA was applied, all the lead may be extracted except for a soil from lead mining area. The differences in extraction efficiencies nay be due to the major cations present in soils which may compete with lead for active sites on EDTA. The total molar amount of major cations extracted was as muck as 20 times more than the added molar amount of EDTA. For some of the soils tested, the extraction efficiency of lead may be affected by being occluded in the Fe and Mn oxides present in the soil. While major cations present in the soil may be one of the factors affecting lead extraction efficiency, the type of lead species present may also play a role. When these factors affect severely, the using of EDTA to extract lead from lead-contaminated soil might be non-effective method.

• Journal of Ocean Engineering and Technology
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• v.28 no.6
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• pp.552-559
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• 2014

In general, the number of underwater transient signals is very limited for research on automatic recognition. Data-dependent feature extraction is one of the most effective methods in this case. Therefore, we suggest WPCC (Wavelet packet ceptsral coefficient) as a feature extraction method. A wavelet packet best tree for each data set is formed using an entropy-based cost function. Then, every terminal node of the best trees is counted to build a common wavelet best tree. It corresponds to flexible and non-uniform filter bank reflecting characteristics for the data set. A GMM (Gaussian mixture model) is used to classify five classes of underwater transient data sets. The error rate of the WPCC is compared using MFCC (Mel-frequency ceptsral coefficients). The error rates of WPCC-db20, db40, and MFCC are 0.4%, 0%, and 0.4%, respectively, when the training data consist of six out of the nine pieces of data in each class. However, WPCC-db20 and db40 show rates of 2.98% and 1.20%, respectively, while MFCC shows a rate of 7.14% when the training data consists of only three pieces. This shows that WPCC is less sensitive to the number of training data pieces than MFCC. Thus, it could be a more appropriate method for underwater transient recognition. These results may be helpful to develop an automatic recognition system for an underwater transient signal.

• The KIPS Transactions:PartB
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• v.13B no.4 s.107
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• pp.409-416
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• 2006

In this paper, we propose an algorithm for extracting text regions from spam-mail images using color layer. The CLTE(color layer-based text extraction) divides the input image into eight planes as color layers. It extracts connected components on the eight images, and then classifies them into text regions and non-text regions based on the component sizes. We also propose an algorithm for recovering damaged text strokes from the extracted text image. In the binary image, there are two types of damaged strokes: (1) middle strokes such as 'ㅣ' or 'ㅡ' are deleted, and (2) the first and/or last strokes such as 'ㅇ' or 'ㅁ' are filled with black pixels. An experiment with 200 spam-mail images shows that the proposed approach is more accurate than conventional methods by over 10%.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.416-420
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• 2002

Various products of karanj (Pongamia glabra) are utilized for industrial, health and animal agriculture applications in the Indian subcontinent. Despite a rich source of protein (CP, 28-34%), karanj cake was found to be slightly bitter in taste and toxic owing to the presence of flavonoid (Karanjin), restricting its safe inclusion in the livestock diets. Feeding trials with raw cake revealed its poor palatability and adverse performance among different categories of livestock including poultry. The present study was, therefore, aimed to detoxify karanj cake by various physico-chemical methods like solvent extraction, water washing, pressure cooking and alkali and acid treatments. The level of residual karanjin in raw and variously processed cake was quantified using high performance liquid chromatography (HPLC). The raw expeller karanj cake was found to contain about 0.19% of karanjin. Though a non-polar solvent, soxhlet extraction of expeller pressed cake with petroleum ether drastically reduced karanjin content (0.01%). Soaking of cake for 24 h in 1% NaOH (w/w) solution was found to reduce karanjin to a major extent with little further benefit by increasing alkali level. Milder alkalies like lime and fertilizer grade urea reduced the karanjin levels marginally. Similar was the case with mineral acids such as HCl and glacial acetic acid. It was, therefore, concluded that solvent extraction of karanj seeds would be the best method of detoxification as well as for more recovery of oil and karanjin.

• Journal of Korea Multimedia Society
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• v.14 no.9
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• pp.1117-1124
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• 2011

This paper proposes a plate number extraction method which uses the information of both the colors and the arrangement of numbers in a vehicle image with complex background. In a number plate, color of the numbers is usually black or white, and numbers are arranged in a row. At first, a raw image is partitioned into the plate number candidate regions and non-interest region. The number candidate regions are thresholded in mean binarization. After eliminating the illegal candidate regions using the aspect ratio of the plate number, the plate number region is finally extracted by using the arrangement information among the numbers. To evaluate the proposed mothed, 292 images are taken in various places and at different times. The experimental results show that the rate of the proposed number regions extraction is about 89.8%, 95.5% for the plate of green and white backgrounds, respectively.

• Environmental Engineering Research
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• v.20 no.2
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• pp.127-132
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• 2015

Perfluorooctane sulfonate (PFOS) and Perfluorooctanoic acid (PFOA) are persistent environmental pollutants, extremely stable, and possibly adversely affect human health. They are widely used in many industries and consumer goods, including sunscreen products. These substances are stable chemicals made of long carbon chains, having both lipid- and water-repellent qualities. The research objectives are (1) to find the most effective method for the preparation of semi-liquid samples by comparing solid phase extraction (SPE) and centrifugation after Pressurized liquid extraction (PLE), and (2) to determine the contamination levels of PFOS and PFOA in waterproof sunscreen samples. All sunscreen samples were analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Sunscreen samples were purchased from domestic and international brands sold in Thailand. Special chemical properties were considered for the selection of samples, e.g., those found in waterproof, sweat resistant, water resistant, and non-stick products. Considering the factors of physical properties, e.g., operation time, chemical consumption, and recovery percentage for selecting methods to develop, the centrifugation method using 2 mL of extracted sample with the conditions of 12,000 rpm and $5^{\circ}C$ for 1 hour after PLE was chosen. The highest concentrations of PFOS and PFOA were detected at 0.0671 ng/g and 21.0644 ng/g, respectively. Even though present concentrations are found at ng/g levels, the daily use of sunscreen products is normally several grams. Therefore, a risk assessment of PFOS and PFOA contamination in sunscreen products is an important concern, and more attention needs to be paid to the long-term effects on human health.

• Korean Journal of Food Science and Technology
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• v.27 no.5
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• pp.658-665
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• 1995

Low yield of a thermostable pectinesterase(TSPE) from citrus fruits has made its detailed study extremely tedious and difficult; therefore, maximizing TSPE extraction is desirable. It is assumed that TSPE is bound to the cell components via ionic linkage and covalent bonds. Therefore, in this study, variations in extraction time, pH, NaCl concentration and commercial enzyme preparations were used to increase the yield of TSPE from Valencia orange. The largest recovery of TSPE, obtained by heating extracted pectinesterase(PE) at $70^{\circ}C\;for\;5{\sim}10$ minute, was achieved using actate buffer(pH 4.14) with 1 M NaCl and 0.2% $Cytolase^{TM}$ 104(a mixture of cellulase, hemicellulase and pectinase; Genecor, Inc). The two aquous phase partitioning with 5.0% Triton X-114 could be used as a tool for separation of thermolabile pectinesterase(TLPE) and TSPE from crude PE. Also, water extraction and $0{\sim}0.3$ ammonium sulfate fractionation could be used for removing non-pectinesterase protein.