• Journal of the Korean Society of Environmental Restoration Technology
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• v.4 no.2
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• pp.45-51
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• 2001

This study was carried out to select the useful plants for the revegetation of flooded slopes in dams, lakes and streams. In this study, four woody plants were investigated for their survival and growth in nursery with various flooding conditions. The results are as follows: Salix gracilistyla showed a very high survival rate and grew continuously under the complete flooding condition. Especially, it grew better under partly flooding condition than non-flooding condition. Amorpha fruticosa showed growth disorder when the flooding period was over 30 days, but the part of stem which was flooded in water adapted itself by branching the adventitious roots. Wisteria floribunda showed respectively high flooding tolerance until 30 days. Lespedeza bicolor were very weak in excessive moisture and flooding conditions. This study confirmed Salix gracilistyla, Amorpha fruticosa and Wisteria floribunda are efficient woody plants for covering the flooded slopes of dam and various impoundment sites.

• Journal of Microbiology and Biotechnology
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• v.10 no.4
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• pp.559-563
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• 2000

A dextransucrase gene (dsrB742) that expresses a dextransucrase to synthesize mostly ${\alpha}-1{\rightarrow}6$ linked dextran with a low amount (3-5%) of ${\alpha}-1{\rightarrow}3$ branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1-kb PstI fragments were ligated with pGEM-3Zf(-) and transformed into E. coli $DH5{\alpha}$. The recombinant clone (pDSRB742) synthesized dextran on an agar plate containing 2% (w/v) sucrose. The dextran synthesized was hydrolyzed with Penicillium endo-dextranase. The hydrolyzate was composed of glucose, isomaltose, isomaltotriose, and branced pentasaccharide. The nucleotide sequence of dsrB742 showed one open reading frame (ORF) composed of 4,524 bp encoding dextrasnsucrase. The deduced amino acid sequence revealed a calculated molecular mass of 168.6 kDa. It also showed an activity band of 184 kKa on a non-denaturing SDS-PAGE (10%). The amino acid sequence of DSRB742 exhibited a 50% similarity with DSRA from L. mesenteroides B-1299, a 70% similarity with DSRS from L. mesenteroides B-512 (F, FMCM) and a 45-56% similarity with Streptococcal GTFs.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.351-355
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• 2005

In the storage roots of sweetpotato (Ipomoea batatas (L.) Lam. cv. Kokei 14), 10 to 20% of starch is essentially unbranched linear amylose and the other major component is branched amylopectin. Amylose is produced by the enzyme GBSSI (granule bound starch synthase I), whereas amylopectin is produced by a concerted action of soluble starch synthase and starch branching enzymes (SBEI and SBEII). We constructed double-stranded RNA (dsRNA) interference vectors of GBSSI and IbSBEII and introduced them into sweetpotato genome via Agrobacterium-mediated gene transformation. The endogenous GBSSI expression was inhibited by dsRNA of GBSSI in 73 % of transgenic plants giving rise to the storage tubers containing amylopectin but not amylose. On the other hand, all sweetpotato plants transformed with dsRNA of IbSBEII contained a larger amount of amylose than the non-transgenic control (up to 25% compared to 10% in the controls). The RNA interference (RNAi) is effectively inhibited the gene expression in thestarch metabolic pathway and modified the characteristics of starch in sweetpotato.

• Applied Microscopy
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• v.46 no.2
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• pp.93-99
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• 2016

The thickness of specimen is an important factor in microscopic researches. Thicker specimen contains more information, but it is difficult to obtain well focused image with precise details due to optical limit of conventional microscope. Recently, a microscope unit that combines improved illumination system, which allows real time three-dimensional (3D) image and automatic z-stack merging software. In this research, we evaluated the usefulness of this unit in observing thick samples; Golgi stained nervous tissue and ground prepared bone, tooth, and non-transparent small sample; zebra fish teeth. Well focused image in thick samples was obtained by processing z-stack images with Panfocal software. A clear feature of neuronal dendrite branching pattern could be taken. 3D features were clearly observed by oblique illumination. Furthermore, 3D array and shape of zebra fish teeth was clearly distinguished. A novel combination of two channel oblique illumination and z-stack imaging process increased depth of field and optimized contrast, which has a potential to be further applied in the field of neuroscience, hard tissue biology, and analysis of small organic structures such as ear ossicles and zebra fish teeth.

• ALGAE
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• v.28 no.2
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• pp.161-171
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• 2013

A morphologically distinct lineage within the Bostrychia moritziana-B. radicans species complex is described as a new species. Bostrychia anomala has thalli with branched monosiphonous filaments with apical cell divisions. The species has terminal tetrasporangial stichidia, each subtending cell bearing tetrasporangia with 2 cover cells. Discharged spores divide transversely, the lower cell first forming a narrow rhizoid and the upper cell forming a monosiphonous shoot. Females have subterminal procarps and males have terminal spermatangial stichidia. Carposporophytes are spherical. Isolates in culture show a pattern of cell death not associated with injury, reminiscent of programmed cell death. Bostrychia anomola shows cell death at intervals along the filaments resulting in division of adjacent cells on either side of the dead cell re-joining the filament; cell division of only one adjacent cell resulting in branching at that site; or filaments fragmenting at the cell death point with adjacent cells forming new apical cells, a means of thallus propagation. The cell death pattern could be a method of filament propagation in the mangrove environment where sexual reproduction is rare.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.21 no.11
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• pp.2886-2896
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• 1996

The Type of Service based multicast routing algorithm is necessary to support efficiently herogeneous applications in ATM network. In this paper I propose the Constrained Multicast Tree with Virtual Destination(DMTVD) heuristic algorithm as least cost multicast routing algorithm. The service is categorized into two types, as delay sensitive and non in CMTVD algorithm. For the delay sensitive service type, the cost optimized route is the Minimum Cost Stenier Tree connecting all the destination node group, virtual destination node group and source node with least costs, subject to the delay along the path being less than the maximum allowable end to end delay. The other side for the non-delay sensitive service, the cost optimized route is the MCST connecting all the multicast groups with least costs, subject to the traffic load is balanced in the network. The CMTVD algorithm is based on the Constrained Multicasting Tree algorithm but regards the nodes branching multiple destination nodes as virtural destination node. The experimental results show that the total route costs is enhanced 10%-15% than the CTM algorithm.

• Journal of Korean Society of Steel Construction
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• v.21 no.6
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• pp.563-574
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• 2009

This paper briefly describes the fundamental strategies--path-tracing, pin-pointing, and path-switching--in the computational elastic bifurcation theory of geometrically non-linear single-load-parameter conservative elastic spatial structures. The stability points in the non-linear elasticity may be classified into limit points and bifurcation points. For the limit points, the path tracing scheme that successively computes the regular equilibrium points on the equilibrium path, and the pinpointing scheme that precisely locates the singular equilibrium points were sufficient for the computational stability analysis. For the bifurcation points, however, a specific procedure for path-switching was also necessary to detect the branching paths to be traced in the post-buckling region. After the introduction, a general theory of elastic stability based on the energy concept was given. Then path tracing, an indirect method of detecting multiple bifurcation points, and path switching strategies were described. Next, some numerical examples of bifurcation analysis were carried out for a trussed stardome, and a pin-supported plane circular arch was described. Finally, concluding remarks were given.

• Transactions of the Korean Society of Automotive Engineers
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• v.15 no.4
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• pp.146-153
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• 2007

The detailed chemistry with reaction mechanism of GRI 2.11, which consists of 49 species and 279 elementary reactions, have been numerically conducted to investigate the flame structure and NO emission characteristics in a non-premixed counterflow flame of blended fuel of $H_2/CO_2/Ar$. The combination of $H_2,\;CO_2$, and Ar as fuel is selected to clearly display the contribution of hydrocarbon products to flame structure and NO emission characteristics due to the breakdown of $CO_2$. Radiative heat loss term is involved to correctly describe the flame dynamics especially at low strain rates. All mechanisms including thermal, $NO_2,\;N_2O$, and Fenimore are also taken into account to separately evaluate the effects of $CO_2$ addition on NO emission characteristics. The increase of added $CO_2$ quantity causes flame temperature to fall since at high strain rates diluent effect is prevailing and at low strain rates the breakdown of $CO_2$ produces relatively populous hydrocarbon products and thus the existence of hydrocarbon products inhibits chain branching. It is also found that the ratio of the contribution by Fenimore mechanism to that by thermal mechanism in the total mole production rate becomes much larger with increase in the $CO_2$ quantity and strain rate, even though the absolute quantity of NO production is deceased. Consequently, as strain rate and $CO_2$ quantity increase, NO production by Fenimore mechanism is remarkably augmented.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.15-22
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• 2009

This study was conducted to examine characteristics of rhizome growth and flowering of white lotus (Nelumbo nucifera) plant in Muan Hoesan-lake for collecting the basic data on the cultivation and the flowering which is growing in Muan Hoesan-lake of Jeonnam province in Korea. We have planted from the seeds and the enlarged rhizomes of the white lotus which were planted in the plastic containers on April 20 and May 4, 2008, and cultivated under rain shelter condition. Rhizome growth pattern and the flowering characteristics of the white lotus were examined on September 4, 2008. Main rhizomes of the white lotus propagated by the seeds and the enlarged rhizomes were branched by 10% and 15%, respectively. Numbers of nodes in the bloomed and non-bloomed rhizomes of the white lotus were over 14 and below 11, respectively. Flower was not observed in the white lotus when propagated with seeds, however, flowers came out up to 80% by the enlarged rhizomes. First flowers were bloomed from the late July and to the early August, and the number of flowers were 4.0~4.4. The first flower stalks without branching of the main rhizome were emerged in 8.8th node, however, that with branching were 8.0th node. Time to flowering from the emergence of flower stalk on the above-ground parts were required 16 days. White flowers from the lotus were 15.3 cm in height and 28.2 cm in width, their longevity was 3.3 days and the averaged flower fresh weight was 29.2 g. The results conclude that optimum number of nodes on the main rhizomes for blooming the white lotus flowers should be over 8 nodes before the rhizome enlargement.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.482-487
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• 2009

The apple rootstock M.26 (Malus pumila) is very popular apple rootstock with semi-dwarf habit and the trees on M.26 produce high quality fruit at a young age. Since it is prone to poor prop ability to soil, however, young trees require staking in windy locations. The rolC gene was introduced into M.26 by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its transformants with dwarfism and enhanced rooting ability. One regenerated transgenic line was confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of rolC gene. The characteristics of transgenic line in vitro were not significantly different from non-transgenic line except for the active root formation and lateral root number. The rolC transgenic line showed reduced stem length and increased root number in vitro. Rooting ability was examined in the isolated greenhouse after mound layering. Compared to non-transgenic M.26, rolC transgenic line showed significantly higher rooting ability. The transgenic line did not show any other observable variation in shoot phenotype compared with non-transgenic line excepting increased branching