• 동의생리병리학회지
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• 제27권1호
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• pp.63-77
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• 2013

The object of this study was to observe anticancer and related immunomodulatory and anticachexic effects of Insamyangyoung-tang aqueous extracts (ISYYTe) on non-small cell lung carcinoma (squamous epithelial carcinoma), NCI-H520, xenograft Balb/c nu-nu nude mice. Changes on the tumor volume and weights, lymphatic organ(spleen and popliteal lymph node), serum interferon (IFN)-${\gamma}$ levels, splenocytes and peritoneal macrophage activities (NK cell activity), splenic tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-10 contents, splenic T-lymphocyte subsets (CD3+, CD4+ and CD8+) and TNF-${\alpha}+$ cells were observed with tumor mass and lymphatic organ histopathology to detect anticancer and immunomodulatory effects. In addition, changes on the body weights, epididymal fat weights and serum IL-6 levels were also detected with the thicknesses of deposited cervical brown adipose tissue and their mean diameters to monitor the tumor-related anticachexic effects. The results obtained in this study suggest that over 50 mg/kg of ISYYTe showed favorable anticancer effects on the NCI-H520 cell xenograft with immunomodulatory and anticachexic effects. However, detail mechanism studies should be conducted in future with the screening of the biological active compounds in this herb.

• Journal of Pharmaceutical Investigation
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• 제32권2호
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• pp.73-80
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• 2002

Cefuroxime axetil is a cephalosporin antibiotic having a high activity against a wide spectrum of Grampositive and Gram-negative microorganisms. It is a cephalosporin antibiotic which exist as 2 diastereoisomers: diastereoisomer A and B. It shows polymorphism of three forms: a crystalline form having a melting point of about $180^{\circ}C$, a substantially amorphous form having a high melting point of about $135^{\circ}C$ and a substantially amorphous form having a low melting point of about 70^{\circ}C$. The crystalline form of cefuroxime axetil is slightly soluble in water because diastereoisomer A has lower solubility than B in water. Substantially amorphous form of which there are no difference in solubility between diastereoisomer A and B has better solubility than crystalline form, but it forms a thicker gel than crystalline form upon contact with an aqueous medium. Based on this reason, cefuroxime axetil is not readily absorbable in the gastrointestinal tract, rendering its bioavailability on oral administration very low. The object of this study was to develop an improved non-crystalline cefuroxime axetil composition having a high physicochemical stability and bioavailability. A non-crystalline cefuroxime axetil solid dispersant showing no peak on a Differential Scanning Calorimetry (DSC) scan is prepared by dissolving cefuroxime axetil and a surfactant in an organic solvent; suspending a water-insoluble inorganic carrier in the resulting solution; and spray drying the resulting suspension to remove the organic solvent, said solid dispersant having an enhanced dissolution and stability of cefuroxime axetil and being useful for the preparation of a pharmaceutical composition for oral administration. Tablet was formulated with this cefuroxime axetil solid dispersant, disintegrants and other ingredients. It disintegrated and dissolved easily and dynamically in dissolution medium, so showed a good dissolution profile.

• 터널과지하공간
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• 제12권4호
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• pp.277-283
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• 2002

본 연구는 안쪽축이 회전하고 바깥쪽 실린더는 정지해있는 반경비가0.52이고 30$^{\circ}$ 경사진 동심 환형관내의 헬리컬 유동 특성에 관한 것이다. 비뉴튼 유체인 UC 수용액과 벤토나이트 수용액을 사용하여 안쪽축이 0~400pm으로 회전할 때 축방향 유동을 완전히 발달시킨 후 축방향 압력손실값을 측정하였다. 또한, 헬리컬 유동의 가시화는 불안정한 파를 관찰하기 위해 수행되었다. 현재 연구 결과는 표면마찰계수에 대해 로스비순(Ro)와 레이놀즈수(Re)의 관계를 나타내었다. 또한, 그 결과들은 유동 불안정성 메카니즘의 존재를 보인다. 축회전수가 증가함에 따라 압력손실이 증가하지만, 그 증가폭은 천이 및 난류영역에서는 레이놀즈수가 증가할수록 감소하며, 회전의 영향으로 유동교란이 증진되어 천이가 촉진된다. 또, 이런 유동교란의 증진은 표면마찰계수값의 증가와 함께 임계 레이놀즈수(Re$_{c}$)를 작게 만든다.

• Advances in Traditional Medicine
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• 제6권3호
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• pp.186-195
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• 2006

The present study was carried out to determine the involvement of opioid and non-opioid receptor and the effect of pH and enzymes on the recently reported antinociceptive activity of aqueous extract of Corchorus olitorius (AECO) leaves using the abdominal constriction test. The extract was prepared by soaking the dried powdered leaves of Corchorus (C.) olitorius in distilled water overnight, and the supernatant obtained was considered as a stock solution with 100% concentration/ strength. The extract, administered subcutaneously in the concentrations/ strength of 10, 50 and 100%, was found to show a significant concentration-independent antinociception. The 50% concentration AECO were further used to study on the above mentioned parameters. The extract exhibited: significant (P < 0.05) decreased in activity when pre-treated (s.c.) against 10 mg/kg naloxonazine, bicuculine (10 mg/kg), phenoxybenzamine (10 mg/kg), 10 mg/kg pindolol, and 5 mg/kg mecamylamme, but not 10 mg/kg naltrindole, 10 mg/kg atropine, respectively; significant (P < 0.05) decreased in activity after pre-treatment against 10% a-amylase, but not 1 % protease or 10% lipase and; significant (P < 0.05) decreased in activity after exposure to alkaline condition (pH between 9 and 13) while maintaining the activity at acidic condition, respectively. The C. olitorius leaves antinociception, which involved, at least in part, activation of $\mu-opioid,\;\alpha-and\;\beta-adrenergic$, and nicotinic receptors, was found to decrease under alkaline condition and in the presence of $\alpha-amylase$.

• 한국재료학회지
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• 제27권3호
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• pp.132-136
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• 2017

We synthesized potassium hexatitanate, ($K_2Ti_6O_{13}$, PT6), with a non-fibrous shape, by acid leaching and subsequent thermal treatment of potassium tetratitanate ($K_2Ti_4O_9$, PT4), with layered crystal structure. By controlling nucleation and growth of PT4 crystals, we obtained splinter-type crystals of PT6 with increased width and reduced thickness. The optimal holding temperature for the layered PT4 was found to be ${\sim}920^{\circ}C$. The length and width of the PT4 crystals were increased when the nucleation and growth time were increased. After a proton exchange reaction using aqueous 0.3 M HCl solution, and subsequent heat treatment at $850^{\circ}C$, the PT4 crystal transformed into splinter-type PT6 crystals. The frictional characteristics of the friction materials show that as the particle size of PT6 increases, the coefficient of friction (COF) and wear amounts of both the friction materials and counter disc increase.

• 한국식품과학회지
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• 제16권1호
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• pp.11-16
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• 1984

멥쌀 및 찹쌀 전분을 상압과 가압가열 및 알칼리 호화한 회석전분 호화액에 대해 유동특성을 검토하였다. 전분농도 $0.2{\sim}1.0%$의 저농도의 호화전분액은 지수법칙 유체의 유동거동을 나타내었으며 아밀로오스 함량에 따라 크게 좌우되어 아밀로오스 함량이 많은 멥쌀은 점성이 낮고 뉴우톤 유체의 경향을 나타내었으며 농도에 대한 영향이 작았다. 찹쌀 전분은 농도가 증가함에 따라 의가소성 성질이 강해졌으며 농도에 따라 급격히 점도가 증가하였다. 가압호화시킨 경우 찹쌀 전분 호화액의 점조도 지수 및 유동 거동 지수는 상압 호화액에 비하여 거의 변화가 없었으나 멥쌀 전분 호화액의 점조도 지수값은 현저히 증가하는 현상을 보였다. 가압 가열호화 및 알칼리 호화 용액은 상압 가열호화에 비해 훨씬 높은 점성을 보였다. 가열 호화 및 알칼리호화액의 겉보기 점도에 대한 온도의 영향을 검토해 본 결과 아레니우스식의 형태에 따름을 알 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제34권11호
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• pp.3444-3450
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• 2013

Three phase hollow fiber-liquid phase microextraction (HF-LPME), which is faster, simpler and uses a more environmentally friendly sample-preparation technique, was developed for the analysis of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in human urine. For the effective simultaneous extraction/concentration of NSAIDs by three phase HF-LPME, parameters (such as extraction organic solvent, pH of donor/acceptor phase, stirring speed, salting-out effect, sample temperature, and extraction time) which influence the extraction efficiency were optimized. NSAIDs were extracted and concentrated from 4 mL of aqueous solution at pH 3 (donor phase) into dihexyl ether immobilized in the wall pores of a porous hollow fiber, and then extracted into the acceptor phase at pH 13 located in the lumen of the hollow fiber. After the extraction, 5 ${\mu}L$ of the acceptor phase was directly injected into the HPLC/UV system. Simultaneous chromatographic separation of seven NSAIDs was achieved on an Eclipse XDB-C18 (4.6 mm i.d. ${\times}$ 150 mm length, 5 ${\mu}m$ particle size) column using isocratic elution with 0.1% formic acid and methanol (30:70) at a HPLC-UV/Vis system. Under optimized conditions (extraction solvent, dihexyl ether; $pH_{donor}$, 3; $pH_{acceptor}$, 13; stirring speed, 1500 rpm; NaCl salt, 10%; sample temperature, $60^{\circ}C$; and extraction time, 45 min), enrichment factors (EF) were between 59 and 260. The limit of detection (LOD) and limit of quantitation (LOQ) in the spiked urine matrix were in the concentration range of 5-15 ng/mL and 15-45 ng/mL, respectively. The relative recovery and precision obtained were between 58 and 136% and below 15.7% RSD, respectively. The calibration curve was linear within the range of 0.015-0.96 ng/mL with the square of the correlation coefficient being more than 0.997. The established method can be used to analyse of NSAIDs of low concentration (ng/mL) in urine.

• 한국지하수토양환경학회지:지하수토양환경
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• 제19권2호
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• pp.38-43
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• 2014

In this study, we evaluated the methylene blue (MB) adsorption potential of non-treated and UV-C pretreated bacterial biomass from aqueous solution. The UV-C pretreatment denature the biomass and has increased overall functional groups when compared to non-treated biomass. The biosorbent was exposed to various pH, biomass dose, and contact time. The results showed that the dried and UV-C pretreated biomass effectively removed MB within 30 min. Dried and UV-C pretreated biomass of Bacillus pseudomycoides JH 2-2 showed a adsorption of 858.2 and 1072.4 mg/g at optimum conditions (pH: 9.0, contact time: 30 min, biomass dose: 1 g/L). Similarly, dried and UV-C pretreated biomass of Citrobacter freundii JH 11-2 showed an adsorption 868.3 and 954 mg/g at optimum conditions (pH: 9.0, contact time: 10 min, biomass dose: 1.5 g/L). The changes in the functional groups of UV-C pretreated biomass could be responsible for enhanced adsorption of MB. The results obtained have shown that non-treated and UV-C pretreated biomass has a high adsorption capacity for MB dye and can be used as a low-cost biosorbent in wastewater treatments.

• 한국식품영양과학회지
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• 제40권2호
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• pp.245-252
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• 2011

신선 채소 중 레드 치커리와 청경채의 미생물학적 안전성 확보를 위해 50 ppm 이산화염소수와 10 kJ/$m^2$ UV-C 조사 병합 처리에 따른 저장 중 미생물 수 및 품질 변화에 미치는 영향을 처리 후 $4{\pm}1^{\circ}C$에서 7일 동안 저장하면서 실험하였다. 레드 치커리와 청경채의 총 호기성 세균 수는 대조구와 비교 시, 이산화염소수와 UV-C 병합 처리구에서 2.64, 2.55 log CFU/g 감소시켰으며, 효모 및 곰팡이는 2.41, 2.00 log CFU/g 감소시켰다. 또한, 레드 치커리와 청경채의 저장 7일 후, 이산화염소수와 UV-C 병합 처리구의 총 호기성 세균수는 3.71, 3.93 log CFU/g으로 대조구와 비교할 때, 2.60, 2.69 log CFU/g의 유의적인(p<0.05) 차이를 나타냈다. 병합 처리는 대조구와 비교하여 레드 치커리와 청경채의 저장 중 Hunter 색도 값에 부정적인 영향을 끼치지 않았다. 관능검사에 있어서도 병합 처리구가 대조구보다 저장 중 관능적 품질 유지에도 효과가 있는 것으로 나타났다. 따라서 본 연구결과, 50 ppm 이산화염소수와 10 kJ/$m^2$ UV-C 조사의 병합 처리가 레드 치커리와 청경채의 저장 중에 오염될 수 있는 위해미생물의 감소와 외관적 품질유지에 효과적인 처리 기술이라고 판단된다.

• 동의생리병리학회지
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• 제24권1호
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• pp.134-142
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• 2010

Although BinSo-San(BSS), a mixed herbal formula consisted of 11 types of medicinal herbs and have been used as anti-inflammatory agent, In the present study, the acute toxicity (single oral dose toxicity) of lyophilized BSS aqueous extracts was monitored in male and female mice after oral administration according to Korea Food and Drug Administration (KFDA) Guidelines (2005-60, 2005). In order to observe the 50% lethal dose ($LD_{50}$), approximate lethal dosage (ALD), maximum tolerance dosage (MTD) and target organs, test articles were once orally administered to female and male ICR mice at dose levels of 2000, 1000, 500, 250 and 0 (control) mg/kg (body wt.) according to the recommendation of KFDA Guidelines (2005-60, 2005). The mortality and changes on body weight, clinical signs and gross observation were monitored during 14 days after dosing according to KFDA Guidelines (2005-60, 2005) with organ weight and histopathology of 12 types of principle organs. We could not find any mortality, clinical signs and changes in the body weights except for dose-independent increases of body weight and gains restricted in 1000 mg/kg of BSS extracts-dosing female group. Hypertrophic changes of lymphoid organs.thymus, spleen and popliteal lymph nodes were detectedat postmortem observation with BSS extracts dose-dependent increases of lymphoid organ weights, and hyperplasia of lymphoid cells in these all three lymphoid organs at histopathological observations. These changes are considered as results of pharmacological effects of BSS extracts or their components, immunomodulating effects, not toxicological signs. In addition, some sporadic accidental findings such as congestion spots, cyst formation in kidney, atrophy of thymus and spleen with depletion of lymphoid cells, and edematous changes of uterus with desquamation of uterus mucosa as estrus cycles were detected throughout the whole experimental groups including both male and female vehicle controls. The significant (p<0.01) increases of absolute weights of kidney and pancreas detected in BSS extracts 1000 mg/kg-treated female group are considered as secondary changes from increases of body weights. The results obtained in this study suggest that the BSS extract is non-toxic in mice and is therefore likely to be safe for clinical use. The LD50 and ALD of BSS aqueous extracts in both female and male mice were considered as over 2000 mg/kg because no mortalities were detected upto 2000 mg/kg that was the highest dose recommended by KFDA and OECD. In addition, the MTD of BSS extracts was also considered as over 2000 mg/kg because no BSS extracts-treatment related toxicological signs were detected at histopathological observation except for BSS or their component-related pharmacological effects, the immunomodulating effects detected in the present study.