• Food Science and Biotechnology
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• 제14권4호
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• pp.503-508
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• 2005

Screening for antilisterial activity was performed in about three thousand isolates of lactic acid bacteria (LAB) from Chinese cabbage kimchi, and finally based on the relatively stronger antilisterial activities eight bacterial strains were selected. The bacteria were further characterized in terms of their tolerance to artificial gastric juice, pH 2.5, bile salts (0.3% oxgall), and to the different NaCl concentrations. Of the isolates, YK005 was especially investigated for its physiological characteristics due to its inhibitory activity against gram-positive Listeria monocytogenes as well as gram-negative Escherichia coli O157:H7, as they have been constantly reported to be resistant against bacteriocins produced by a number of LAB strains. YK005 was found to be rod-shaped, $3.8\;{\mu}m$ long ${\times}\;0.5\;{\mu}m$ wide, non-sporeforming, non-motile, catalase-negative, and produced gas from glucose (heterolactic). Based on the biochemical data obtained by API 50 CHL medium, the isolate was tentatively identified as Lactobacillus brevis. To validate the result obtained by the biochemical identification, rRNA-based PCR experiments using a pair of species-specific primers for L. brevis were conducted and a single band of 1400 bp was observed, which strongly indicated that YK005 belongs to L. brevis. The LAB isolates are potentially exploited as human probiotic organisms and are employed to control some food-borne pathogens like L. monocytogenes.

• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.15-20
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• 2016

Toxoplasmosis is an important zoonotic disease that can cause abortion in humans and animals. The aim of this study was isolation and subsequent genotyping of Toxoplasma gondii isolates in ovine aborted fetuses. During 2012-2013, 39 ovine aborted fetuses were collected from sheep flocks in Khorasan Razavi Province, Iran. The brain samples were screened for detection of the parasite DNA by nested PCR. The positive brain samples were bioassayed in Webster Swiss mice. The serum samples of mice were examined for T. gondii antibodies by IFAT at 6 weeks post inoculation, and T. gondii cysts were searched in brain tissue samples of seropositive mice. The positive samples were genotyped by using a PCR-RLFP method. Subsequently, GRA6 sequences of isolates were analyzed using a phylogenetic method. The results revealed that T. gondii DNA was detected in 54% (20/37, 95% CI 38.4-69.0%) brain samples of ovine aborted fetuses. In bioassay of mice, only 2 samples were virulent and the mice were killed at 30 days post inoculation, while the others were non-virulent to mice. The size of cysts ranged $7-22{\mu}m$. Complete genotyping data for GRA6 locus were observed in 5 of the 20 samples. PCR-RLFP results and phylogenetic analysis revealed that all of the isolated samples were closely related to type I. For the first time, we could genotype and report T. gondii isolates from ovine aborted fetuses in Khorasan Razavi Province, Iran. The results indicate that the T. gondii isolates are genetically related to type I, although most of them were non-virulent for mice.

• 한국연초학회지
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• 제2권1호
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• pp.17-27
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• 1980

143개의 여러가지 시료에서 니코틴 분해세균의 분포를 조사하고 니코틴 배지에서 생장능이 우수한 34균주를 선별하여 균주의 생물학적 특성과 니코틴 분해력을 조사함과 동시에 니코틴 분해력이 가장 우수한 균주를 선정하여 동정하였다. 니코틴 분해세균은 유기물이 풍부한 토양과 담배씨, 부리에 많이 분포되어 있었고 선별된 34균주는 Arthrobacter 4주, Pseudomonas (non-pigmented) 11주, Pseudomonas (Pigmented) 2주, Alkaligenes 6주, Chromobacter 5주, Listeria 2주, Achromobacter 4주 등으로 분류되었다. 니코틴 분해력에 있어서는, Pseudomonas와 Alkaligenes가 다른 속(genus)보다 높고, 서식지 별로 비교해 보면 담배씨와 뿌리에서 분리된 균주가 우수했으며 가장 우수한 균주는 97.l%의 분해율을 나타내는 균주번호 NCT27로서 Pseudomonas putida로 동정되었다.

• 한국정보통신학회논문지
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• 제16권5호
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• pp.1023-1028
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• 2012

본 논문에서는 비접촉 마이크로웨이브 프루브 시스템의 I/Q demodulator를 위한 MMIC (Monolithic Microwave Integrated Circuit) mixer chip을 GaAs p-HEMT 공정의 Schottky 다이오드를 이용하여 설계 및 제작하였다. 프루브 시스템의 I/Q demodulator 구조를 단순화하기 위해 single balanced 구조의 mixer를 채택하였다. Single balanced mixer에서 $90^{\circ}$hybrid coupler와 ${\lambda}/4$ 전송선로를 이용하여 $180^{\circ}$hybrid를 설계하였으며 이를 MIM 커패시터와 spiral 인덕터를 이용하여 구현함으로써 mixer chip의 크기를 줄일 수 있었다. On-wafer 측정 결과, 본 논문의 MMIC mixer는 1650MHz ~ 2050MHz의 RF 및 LO 주파수 대역을 포함하고 있으며, 응용 주파수 대역 내에서 RF 및 LO의 변화에 대해 약 12dB 이하의 평탄한 변환손실(conversion loss) 특성을 나타내었다. 또한, MMIC mixer chip은 $2.5mm{\times}1.7mm$의 초소형 크기를 가지며 LO-IF 및 RF-IF의 격리도는 각각 43dB 및 23dB 이상의 특성을 나타내었다.

• 전기전자학회논문지
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• 제23권3호
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• pp.962-970
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• 2019

본 논문에서는 자기구동 동기스위치를 이용한 비절연 고효율 고전압출력 DC-DC 컨버터를 제안한다. 제안하는 컨버터는 전통적인 승압형 DC-DC 컨버터 구조에 탭형 인덕터를 적용함으로써 고전압출력을 달성하며 주스위치부에 무손실 커패시터-다이오드(LCD, lossless capacitor-diode) 스너버를 적용하여 스위치 전압스트레스를 저감한다. 그리고 출력부에 다이오드 대신에 동기스위치를 적용함으로써 역회복 문제를 해결하고 고효율을 달성한다. 제안한 컨버터의 동기스위치는 자기구동방식을 이용하고 단순한 구조를 가진다. 본 논문에서는 제안한 컨버터의 동작원리를 먼저 설명하고, 후에 컨버터 프로토타입의 설계예를 제시한다. 그리고 설계된 회로파라미터로 제작된 프로토타입의 실험결과로써 제안한 컨버터의 특성을 보인다.

• Journal of Chest Surgery
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• 제56권2호
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• pp.128-135
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• 2023

Background: Pneumonia caused by severe acute respiratory syndrome coronavirus 2 can cause acute respiratory distress syndrome, often requiring prolonged mechanical ventilation and eventually tracheostomy. Both procedures occur in isolation units where personal protective equipment is needed. Additionally, the high bleeding risk in patients with extracorporeal membrane oxygenation (ECMO) places a great strain on surgeons. We investigated the clinical characteristics and outcomes of percutaneous dilatational tracheostomy (PDT) in patients with coronavirus disease 2019 (COVID-19) supported by ECMO, and compared the outcomes of patients with and without ECMO. Methods: This retrospective, single-center, observational study included patients with severe COVID-19 who underwent elective PDT (n=29) from April 1, 2020, to October 31, 2021. The patients were divided into ECMO and non-ECMO groups. Data were collected from electronic medical records at Ajou University Hospital in Suwon, Korea. Results: Twenty-nine COVID-19 patients underwent PDT (24 men [82.8%] and 5 women [17.2%]; median age, 61 years; range, 26-87 years; interquartile range, 54-71 years). The mean procedure time was 17±10.07 minutes. No clinically or statistically significant difference in procedure time was noted between the ECMO and non-ECMO groups (16.35±7.34 vs. 18.25±13.32, p=0.661). Overall, 12 patients (41.4%) had minor complications; 10 had mild subdermal bleeding from the skin incision, which was resolved with local gauze packing, and 2 (6.9%) had dislodgement. No healthcare provider infection was reported. Conclusion: Our PDT approach is safe for patients and healthcare providers. With bronchoscopy assistance, PDT can be performed quickly and easily even in isolation units and with acceptable risk, regardless of the hypo-coagulable condition of patients on ECMO.

• Journal of Animal Science and Technology
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• 제66권5호
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• pp.1021-1033
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• 2024

Exosomes have been extensively studied as disease biomarker in humans, given their role in transporting bioactive molecules. However, despite the great potential of exosomes as noninvasive diagnostic markers and therapeutic nanocarriers for bovine diseases, few studies have been conducted on bovine exosome. Thus, this study aimed to quantitatively and qualitatively compare three isolation methods to identify a suitable method for bovine serum. Exosomes were isolated using ultracentrifugation alone (UC), a combination of ultracentrifugation and size exclusion chromatography (US), or membrane affinity-based exoEasy kit (EE). Isolated particles were evaluated using a range of complementary techniques. Transmission electron microscopy showed that all three isolation methods resulted in particles with a cup-shaped morphology. The particle concentration measured by nanoparticle trafficking analyzer of US was lower compared to those of UC and EE method. As a result of immunoblotting, exosome markers including TSG101, CD81, and HSP70 were detected in US particles, while in UC and EE, only TSG101 expression was confirmed. Particles isolated from UC and EE showed a contamination with the blood protein albumin, whereas particles from US did not show albumin contamination. In addition, to evaluate the possibility of using exosomes as biomarkers, the profiles of the small RNA in the exosomes were compared using the bioanalyzer 2100. As a result, in the EE method, the band of small RNA (25-200 nt) was most prominent, and in the US methods, a distinct band was observed in the small RNA range. Collectively, the purity of exosomes without non-exosomal contamination was highest in the US method. However, for the detection of small RNA, the EE method was found to be the most suitable. Therefore, the results suggest that the optimal isolation method varies depending on the specific purpose of exosome isolation.

• 한국축산식품학회지
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• 제37권5호
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• pp.654-662
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• 2017

Extracellular microvesicles are membranous nano-sized cellular organelles secreted by a variety of cells under normal and pathological conditions and heterogeneous in size ranging from 30 nm to $1{\mu}m$. They carry functional microRNAs that can influence immunity and development. For a particular application of microvesicles, choice of isolation method is particularly important; however, their isolation methods from colostrum in particular have not been described clearly. In this work, differential ultracentrifugation as a conventional method, ultracentrifugation with some modification such as additional precipitations, ultrafiltration, sucrose gradient separation and ExoQuick$^{TM}$ as a commercial reagent were compared. The goal was to compare mainly microvesicular total microRNA yield, distribution and purity among the methods then select the best isolation method for bovine colostrum microvesicles based largely on microRNA yield with the view of applying the vesicles in work where vesicular microRNA cargo is the target bioactive component. Highest yields for vesicular microRNA were obtained using conventional methods and among them, subsequent ultracentrifugation with 100,000 g and 135,000 g conventional method 2 was selected as it had the highest RNA to protein ratio indicating that it pelleted the least protein in relation to RNA an important factor for in vivo applications to assess microvesicle functionalities without risk of contaminating non-vesicular biomaterial. Microvesicles isolated using conventional method 2 were successfully internalized by cells in vitro showing their potential to deliver their cargo into cells in vitro and in vivo in case of functional studies.

• 한국강구조학회 논문집
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• 제22권1호
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• pp.43-53
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• 2010

면진기술은 건축물의 구조적 피해를 줄이고 진동에 민감한 구조물의 내진성능을 향상시키는 가장 효과적인 방법 중의 하나이다. 하지만 면진구조시스템에서 지진에너지를 흡수하는 댐퍼의 재료로서 자주 사용되었던 납의 환경오염문제가 대두되면서, 최근에는 친환경 재료인 강재를 사용하는 경우가 증가하고 있다. 본 연구에서는 면진시스템용 에너지흡수장치인 U형 강재댐퍼를 대상으로 비선형 유한요소해석을 수행하고 SS400 강재와 고인성강재를 사용한 댐퍼의 해석결과를 비교하여 사용강재의 재료적 특성의 차이가 댐퍼의 변형능력에 미치는 영향을 분석하며, 고인성강재를 사용한 댐퍼의 형상비, 두께, 폭에 따른 변형능력을 파악한다. 또한 고인성강재를 사용한 U형 강재댐퍼의 응력분포를 분석하여 응력분포에서 나타내는 문제점을 개선하기 위한 대책으로 댐퍼의 일부 구간에 개구부를 둔 새로운 형상을 제시하고 형상에 따른 변형능력, 내력특성 및 응력분포를 고려하여 최적형상과 설계식을 제안하고자 한다.

• 터널과지하공간
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• 제11권4호
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• pp.362-367
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• 2001

지반에서 겨울에 발생할 수 있는 단단한 상부층은 방진벽의 진동차단성능을 변화시킬 수 있다. 본 논문은 상부에 단단한 층이 존재하는 층진 지반(layered soil)에서 표면파의 진동전파와 강성 방진벽의 진동차단 성능에 관한 것이다. 연구는 이차원문제로, 경계요소법을 이용한 수치해석적 방법에 의해 수행되었으며, 진동원은 수직방향으로 조화가 진을 받는 줄기초이다. 검토된 지반은 세 가지로, 균질의 반무한 지반과, 단단한 상부층의 두께를 달리하는 두 개의 지반이다. 단단한 상부층을 갖는 지반에서는 굴절의 지반과 비교하여 아주 커다란 진폭의 감소가 나타났으며, 진동전파 속도의 경우, 물성치에 의해 계산으로 구해지는 진동전파 속도만큼의 크기가 나타나지 않았다. 더욱이 동결지반에서의 진폭은 비동결지반에서 보다 거리에 따라 아주 작은 값이 구해졌다.