Parasites, Hosts and Diseases

  • 제54권1호
  • /
  • Pages.15-20
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  • 2016
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Isolation and Genotyping of Toxoplasma gondii Strains in Ovine Aborted Fetuses in Khorasan Razavi Province, Iran

  • Danehchin, Leila (Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad) ;
  • Razmi, Gholamreza (Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad) ;
  • Naghibi, Abolghasem (Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad)
  • 투고 : 2015.10.03
  • 심사 : 2015.12.09
  • 발행 : 2016.02.29
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초록

Toxoplasmosis is an important zoonotic disease that can cause abortion in humans and animals. The aim of this study was isolation and subsequent genotyping of Toxoplasma gondii isolates in ovine aborted fetuses. During 2012-2013, 39 ovine aborted fetuses were collected from sheep flocks in Khorasan Razavi Province, Iran. The brain samples were screened for detection of the parasite DNA by nested PCR. The positive brain samples were bioassayed in Webster Swiss mice. The serum samples of mice were examined for T. gondii antibodies by IFAT at 6 weeks post inoculation, and T. gondii cysts were searched in brain tissue samples of seropositive mice. The positive samples were genotyped by using a PCR-RLFP method. Subsequently, GRA6 sequences of isolates were analyzed using a phylogenetic method. The results revealed that T. gondii DNA was detected in 54% (20/37, 95% CI 38.4-69.0%) brain samples of ovine aborted fetuses. In bioassay of mice, only 2 samples were virulent and the mice were killed at 30 days post inoculation, while the others were non-virulent to mice. The size of cysts ranged $7-22{\mu}m$. Complete genotyping data for GRA6 locus were observed in 5 of the 20 samples. PCR-RLFP results and phylogenetic analysis revealed that all of the isolated samples were closely related to type I. For the first time, we could genotype and report T. gondii isolates from ovine aborted fetuses in Khorasan Razavi Province, Iran. The results indicate that the T. gondii isolates are genetically related to type I, although most of them were non-virulent for mice.

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참고문헌

  1. Dubey JP. Toxoplasmosis of Animals and Humans, 2nd ed. Boca Raton, Florida, USA. CRC Press. 2010, pp. 1-14.
  2. Buxton D, Maley SW, Wright SE, Rodger S, Bartley P, Innes EA. Toxoplasma gondii and ovine toxoplasmosis: new aspects of an old story. Vet Parasitol 2007; 149: 25-28. https://doi.org/10.1016/j.vetpar.2007.07.003
  3. Dubey JP. Toxoplasmosis in sheep-the last 20 years. Vet Parasitol 2009; 163: 1-14. https://doi.org/10.1016/j.vetpar.2009.02.026
  4. Sibley LD, Boothroyd JC. Virulent strains of Toxoplasma gondii comprise a single clonal lineage. Nature 1992; 359: 82-85. https://doi.org/10.1038/359082a0
  5. Darde ML, Bouteille B, Pestre-Alexandre M. Isoenzyme analysis of 35 Toxoplasma gondii isolates and the biological and epidemiological implications. J Parasitol 1992; 78: 786-794. https://doi.org/10.2307/3283305
  6. Su C, Zhang X, Dubey JP. Genotyping of Toxoplasma gondii by multilocus PCR-RFLP markers: a high resolution and simple method for identification of parasites. Int J Parasitol 2006; 36: 841-848. https://doi.org/10.1016/j.ijpara.2006.03.003
  7. Su C, Shwab E K, Zhou P, Zhu XQ, Dubey JP. Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii. Parasitology 2010; 137: 1-11
  8. Ajzenberg D, Banuls AL, Tibayrenc M, Darde ML. Microsatellite analysis of Toxoplasma gondii shows considerable polymorphism structured into two main clonal groups. Int J Parasitol 2002; 32: 27-38. https://doi.org/10.1016/S0020-7519(01)00301-0
  9. Ajzenberg D, Collinet F, Mercier A, Vignoles P, Darde ML. Genotyping of Toxoplasma gondii isolates with 15 microsatellite markers in a single multiplex PCR assay. J Clin Microbiol 2010; 48: 4641-4645. https://doi.org/10.1128/JCM.01152-10
  10. Khan A, Dubey JB, Su C, Ajioka JW, Rosenthal BM, Sibley LD. Genetic analyses of atypical Toxoplasma gondii strains reveal a fourth clonal lineage in North America. Int J Parasitol 2011; 41:645-655. https://doi.org/10.1016/j.ijpara.2011.01.005
  11. Darde ML, AJzenberg D, Su S. Molecular epidemiology and population structure of Toxoplasma gondii. In Weiss LM, Kim K eds, Toxoplasma gondii The Model Apicomplexan-Perspectives and Methods. 2nd ed. Amesterdam, The Netherlands. Elsevier. 2014, pp. 66-93.
  12. Darde ML. Biodiversity in Toxoplasma gondii. Curr Top Microbiol Immunol 1996; 219: 27-41.
  13. Owen MR, Trees AJ. Genotyping of Toxoplasma gondii associated with abortion in sheep. J Parasitol 1999; 85: 382-384. https://doi.org/10.2307/3285654
  14. Jungersen G, Jensen L, Rask MR, Lind P. Non-lethal infection parameters in mice separate sheep type II Toxoplasma gondii isolates by virulence. Comp Immunol Microbiol Infect Dis 2002; 25: 187-195. https://doi.org/10.1016/S0147-9571(01)00039-X
  15. Dumetre A, Ajzenberg D, Rozette L, Mercier A, Darde ML. Toxoplasma gondii infection in sheep from Haute-Vienne, France: seroprevalence and isolate genotyping by microsatellite analysis. Vet Parasitol 2006; 142: 376-379. https://doi.org/10.1016/j.vetpar.2006.07.005
  16. Dubey JP, Sundar N, Hill D, Velmurugan GV, Bandini LA, Kwok OC, Majumdar D, Su C. High prevalence and abundant atypical genotypes of Toxoplasma gondii isolated from lambs destined for human consumption in the USA. Int J Parasitol 2008; 38: 999-1006. https://doi.org/10.1016/j.ijpara.2007.11.012
  17. Edwards JF, Dubey JP. Toxoplasma gondii abortion storm in sheep on a Texas farm and isolation of mouse virulent atypical genotype T. gondii from an aborted lamb from a chronically infected ewe. Vet Parasitol 2013; 192: 129-136. https://doi.org/10.1016/j.vetpar.2012.09.037
  18. Ragozo, AM, Pena HF, Yai LE, Su C, Gennari SM. Genetic diversity among Toxoplasma gondii isolates of small ruminants from Brazil: novel genotypes revealed. Vet Parasitol 2010; 170: 307-312. https://doi.org/10.1016/j.vetpar.2010.02.024
  19. Da Silva RC, Langoni H, Su C, Da Silva AV. Genotypic characterization of Toxoplasma gondii in sheep from Brazilian slaughterhouses: new atypical genotypes and the clonal type II strain identified. Vet Parasitol 2011; 175: 173-177. https://doi.org/10.1016/j.vetpar.2010.09.021
  20. Chessa G, Chisu V, Porcu R, Masala G. Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy. Parasite 2014; 21: 6. https://doi.org/10.1051/parasite/2014007
  21. Gebremedhin EZ, Abdurahaman M, Tessema TS, Tilahun G, Cox E, Goddeeris B, Dorny P, De Craeye S, Darde ML, Ajzenberg D. Isolation and genotyping of viable Toxoplasma gondii from sheep and goats in Ethiopia destined for human consumption. Parasit Vectors 2014; 4: 425.
  22. Sharifi M, Sarvi Sh, Shokri A, Hosseini-Teshnizi S, Rahimi MT, Mizani, A, Ahmadpour E, Daryani A. Toxoplasma gondii infection among sheep and goats in Iran: a systematic review and metaanalysis. Parasitol Res 2015; 114: 1-16.
  23. Zia-Ali N, Fazaeli A, Khoramizadeh M, Ajzenberg D, Darde M, Keshavarz-Valian H. Isolation and molecular characterization of Toxoplasma gondii strains from different hosts in Iran. Parasitol Res 2007; 101: 111-115. https://doi.org/10.1007/s00436-007-0461-7
  24. Habibi GR, Imani AR, Gholami MR, Hablolvarid MH, Behroozikhah AM, Lotfi M, Kamalzade M, Najjar E, Esmaeil-Nia K, Bozorgi S. Detection and identification of Toxoplasma gondii type I infection in sheep aborted fetuses in Qazvin Province of Iran. Iran J Parasitol 2012; 7: 64-72.
  25. Razmi GR, Ghezi K, Mahooti A, Naseri Z. A serological study and subsequent isolation of Toxoplasma gondii from aborted ovine fetuses in Mashhad area, Iran. J Parasitol 2010; 96: 812-814. https://doi.org/10.1645/GE-2428.1
  26. Rassouli M, Razmi GR, Basami M, Movassaghi AR, Azizzadeh M. Study on ovine abortion associated with Toxoplasma gondii in affected herds of Khorasan Razavi Province, Iran based on PCR detection of fetal brains and maternal serology. Parasitology 2011; 138: 691-697. https://doi.org/10.1017/S0031182011000205
  27. Evans HE, Sack WO. Prenatal development of domestic and laboratory mammals: growth curves, external features and selected refrences. Anat Histol Embryo 1973; 12: 11-45
  28. Burg JL, Grover CM, Pouletty P, Boothroyd JC. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol 1989; 27: 1787-1792.
  29. Razmi GR, Rahbari S. Comparative study of three tests (modified direct agglutination test, indirect immunofluorescent antibody test and dye test) for detection of antibodies to Toxoplasma gondii in sheep sera. Iran J Vet Res 2001; 1: 12-19.
  30. Fazaeli A, Carter PE, Darde ML, Pennington TH. Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis. Int J Parasitol 2000; 30: 637-642. https://doi.org/10.1016/S0020-7519(00)00036-9
  31. Moazeni Jula F, Moazeni Jula G, Nozarian N, Hashemzadeh F. A serological and molecular study on Toxoplasma gondii in sheep and goats in Tabriz. Arch Razi Inst 2013; 68: 29-35.
  32. Dubey JP, Lindsay DS, Speer CA. Structures of Toxoplasma gondii tachyzoites, bradyzoites, and sporozoites and biology and development of tissue cysts. Clin Microbiol Rev 1998; 11: 267-299.
  33. Hooshyar H, Rostamkhani P, Arbabi M. Study on growth of Toxoplasma gondii tissue cyst in laboratory mouse. Jundishapur J Microbiol 2009; 2: 140-143.
  34. Messaritakis I, Detsika M, Koliou M, Sifakis S, Antoniou M. Prevalent genotypes of Toxoplasma gondii in pregnant women and patients from Crete and Cyprus. Am J Trop Med Hyg 2008; 2: 205-209.
  35. Zakimi S, Kyan H, Oshiro M, Sugimoto C, Xuenan X, Fujisaki K. Genetic characterization of GRA6 genes from Toxoplasma gondii from pigs in Okinawa, Japan. J Vet Med Sci 2006; 68: 1105-1107. https://doi.org/10.1292/jvms.68.1105

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  4. Molecular Diagnosis and Pathological Study of Toxoplasma gondii in Aborted Caprine and Ovine Fetuses in Borderline of Iran-Iraq vol.65, pp.1, 2020, https://doi.org/10.2478/s11686-019-00147-4
  5. Molecular Genotyping of Toxoplasma gondii in Sheep Aborted Fetuses Reveals Predominance of Type I Infection in Southwest of Iran vol.15, pp.3, 2020, https://doi.org/10.18502/ijpa.v15i3.4202
  6. Molecular and serological detection and of Toxoplasma gondii in small ruminants of southwest Iran and the potential risks for consumers vol.16, pp.2, 2016, https://doi.org/10.1007/s00003-020-01306-w