• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.751-758
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• 2004

The NCS(Non-Cariogenicity Sugar) from Bacillus sp. S-1013 was purified by cold acetone and methanol precipitation, and DEAE-cellulose ion-exchange and Sephadex G-100 column chromatographies, to yield an amorphous yellow syrup. The melting point and $[\alpha]_D^{20}$ were 155-$157^{\circ}C$ and +53, respectively. Instrumental analyses such as FT-IR, $^1H-NMR, and ^{13}C-NMR$ showed that the NCS contained an O-H group, C-H, C=O, $NH_2$, anomeric carbon, anomeric proton, N-acetylgalactose, fucose, and neuramic acid, thus, the NCS was determined to be a trisaccharide of Fuc($1\longrightarrow4$)GalNAc($2\longrightarrow6$) NeuAc.

• 한국미생물·생명공학회지
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• 제34권3호
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• pp.235-243
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• 2006

비우식성 당 NCS(Fuc($1{\to}4$)galNAc($2{\to}6$)NeuAc)를 생산하는 미생물을 동정하고 이 미생물이 생산하는 비우식성 당의 생산조건을 최적화하기 위하여 본 연구를 수행하였다. NCS를 생산하는 균주는 형태학적, 생화학적, 화학분류학적 특성 및 16S-rRNA 염기서열 결정을 통하여 Bacillus sp. S-1013로 동정되었다. 또한, 이 균주에 의한 NCS의 최적 배지조성은 soluble starch 3.0%, dextrin 1.5%, yeast extract 0.5%, pepton 1.0%, $K_{2}HPO_4$ 0.2%였으며, 최적온도 및 pH는 각각 25와 11.0이였다. 최적배지에서 NCS 및 균체의 생산은 기본배지에 비해 각각 4.24배 및 2.67배 증가하였다.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.759-765
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• 2004

The NCS inhibited the activity of glucosyltransferase which was produced by Streptococcus mutans JC-2, and the rate of inhibition at $100\muM<$ and $200\muM$ were 74.0% and 99.8%, respectively. It was stable in alkali condition, but unstable in acid condition. It was also stable up to $80^{\circ}C$. The kinetic study of the inhibition by NCS was carried out by Lineweaver-Burk plot and Dixon plot. It was non-competitive inhibition, determined by the two plots and $K_i$ and $K_i$ values were $15\muM$ and $19.3\muM$ respectively. The NCS did not show cytotoxicity against human gingival cells at $K_i$ ($15\muM$, $150\muM$, $1,500\mu$ M) concentrations. It had less cytotoxicity than chlohexidin, which has usually been used as the agent of anticaries. To evaluate the industrial applicability of the NCS, human pluck tooth was used. The inhibitory rates of tooth calcification and calcium ion elution by the NCS were 41 % and 2.5 times, respectively. These results suggested that NCS from Bacillus sp. S-1013 is an efficient anticaries agent.

• KSBB Journal
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• 제13권3호
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• pp.331-335
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• 1998

Erythritol is a four-carbon sugar alcohol with a low calorific value and non-cariogenicity. Erythritol is a new functional sweetener which can be used as sugar alternative. Erytheitol dose not cause discomfort such as diarrhoea and flatulence upon ingestion. The purpose of this study is to develope a novel process of erythritol economically in a large scale. To obtain a high erythritol producer, we have screened 3500 colonies from molasses, honey and honey combs. We have selected 40 erythritol-producing microorganisms, one of which yields 140g/L erythritol in 40% glucose medium. We have tested this strain in 5L fermentor to examine the fermentation characteristics. Results of fermentation show that the erythritol production was about 1.4g/L$.$hr in 400g/L glucose media with a 42% conversion. Further improvements require mutation for a higher producer, process optimization to reduce glycerol, and suppression of excessive foaming.