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Identification of Alkalophilic Bacillus sp. S-1013 Producing Non-Cariogenicity Sugar Fuc($1{\to}4$)gaINAc($2{\to}6$)NeuAc and Optimization of Culture Condition for Its Production  

Ryu Il-Hwan (College of Life Science and Natural Resources, Wonkwang University)
Kim Sun-Sook (Department of Dental Hygienic, Suwon Wonen's College)
Lee Kap-Sang (College of Life Science and natural Resources, Wonkwang University)
Lee Eun-Sook (Department of Dental Hygienic, Gimcheon College)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.3, 2006 , pp. 235-243 More about this Journal
Abstract
The study was performed to identification of producing microbe Non-Cariogenicity Sugar (NCS; Fuc($1{\to}4$) gaINAc($2{\to}6$)NeuAc) with anti-caries activity, and to optimization of production condition. A typical strain which produced the NCS was identified alkalophilic Bacillus sp. S-1013 through the results of morphological, biochemical and chemotaxonomic characteristics and 16S rDNA sequencing. The optimal medium composition for the maximal production of the NCS from alkalophilic Bacillus sp. S-1013 was as follow: soluble starch 30 g, dextrin 15 g, yeast extract 5 g, peptone 10 g, $K_{2}HPO_4$ 2 g in a liter of distilled water. Optimal temperature and pH were 25 and 11.0, respectively. The highest production of NCS was shown 60 hrs cultivation using the optimal medium, and then NCS productivity and dry cell weight of culture broth increased 4.24 and 2.67 time than initial medium, respectively.
Keywords
Non-Cariogenicity Sugar; alkalophilic Bacillus sp; optimization;
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