• 문화기술의 융합
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• 제4권4호
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• pp.367-372
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• 2018

본 논문은 무선 센서 네트워크의 경계노드를 효과적으로 검출하는 알고리즘을 제안한다. 경계노드는 센서 네트워크의 외부 경계 또는 홀의 경계에 존재하는 센서이다. 제안한 알고리즘은 센서의 위치정보만을 이용하여 네트워크의 경계노드를 검출한다. 또한 검출 속도를 향상시키기 위해 센서는 거리에 따라 근접한 센서와의 중첩영역을 먼저 계산한다. 알고리즘의 동작을 검증하기 위해 시뮬레이션을 수행하였다. 시뮬레이션에서 현실 세계를 최대한 반영하기 위해 정해진 영역에 여러 개의 장애물을 설치하고 센서의 개수를 500~1500개 범위에서 다르게 배치하였다. 시뮬레이션을 통하여 제안한 알고리즘이 네트워크 외부 및 홀의 경계에 존재하는 경계노드들을 효과적으로 검출하는 것을 확인하였다.

• Journal of Plant Biotechnology
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• 제44권2호
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• pp.171-177
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• 2017

우리나라에서 멸종위기 해안식물로 지정된 갯봄맞이의 캘루스배양을 통한 재분화 프로토콜을 보고한다. 마디배양을 통해 육성한 기내 유식물체의 절편은 유전자형, 절편의 종류, 광조건 및 2,4-D 첨가농도에 따라 캘루스 형성에 상이한 반응을 보였다. 특히, 다른 처리와는 무관하게 연속 암배양이 절편으로부터 캘루스 유도에 결정적인 영향을 미쳤다. 2,4-D 0.1-3.0mg/L 농도 범위의 배지에서 유도된 캘루스를 동일한 조성의 배지로 이식하여 암배양했을 때왕성하게 캘루스가 증식하였다. 연속 암조건에서 zeatin 1.0mg/L 첨가배지에 'Pistachio' 유전자형의 캘루스를 배양했을 때만 유일하게 정상적인 신초가 재분화 되었고, 이 때 신초는 캘루스로부터 형성된 마디구조에서 발달하였으며 신초의 재분화율은 24.4%였다. 재분화 신초를 식물호르몬 무첨가 배지로 이식하여 명배양 했을 때 지상부 녹화와 더불어 뿌리가 발생하여 유식물체로 발달하였다. 기내 유식물체를 기 보고된 마디배양 프로토콜(Bae et al. 2016)에 따라 배양했을 때 효과적으로 유식물체를 증식시킬 수 있었다. 기외 순화시킨 유식물체는 내륙 자연 환경하에서 성숙식물체로 생육하였으며 이듬 해 4월에 개화하였다. 본 연구의 결과는 Bae et al. (2016)이 제안한 마디배양 프로토콜과 통합하여 운용될 수 있고, 통합된 프로토콜은 갯봄맞이의 기내 번식 시스템으로서 갯봄맞이 자생지 복원에 기여할 수 있을 것이다.

• 한국컴퓨터정보학회논문지
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• 제24권4호
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• pp.169-176
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• 2019

Wireless sensor networks are used to monitor and control areas in a variety of military and civilian areas such as battlefield surveillance, intrusion detection, disaster recovery, biological detection, and environmental monitoring. Since the sensor nodes are randomly placed in the area of interest, separation of the sensor network area may occur due to environmental obstacles or a sensor may not exist in some areas. Also, in the situation where the sensor node is placed in a non-relocatable place, some node may exhaust energy or physical hole of the sensor node may cause coverage hole. Coverage holes can affect the performance of the entire sensor network, such as reducing data reliability, changing network topologies, disconnecting data links, and degrading transmission load. It is possible to solve the problem that occurs in the coverage hole by finding a coverage hole in the sensor network and further arranging a new sensor node in the detected coverage hole. The existing coverage hole detection technique is based on the location of the sensor node, but it is inefficient to mount the GPS on the sensor node having limited resources, and performing other location information processing causes a lot of message transmission overhead. In this paper, we propose an Adjacent Matrix-based Hole Coverage Discovery(AMHCD) scheme based on connectivity of neighboring nodes. The method searches for whether the connectivity of the neighboring nodes constitutes a closed shape based on the adjacent matrix, and determines whether the node is an internal node or a boundary node. Therefore, the message overhead for the location information strokes does not occur and can be applied irrespective of the position information error.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.263-270
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• 2006

Pongamia pinnata Pierre is a tree legume, having potential in production of raw material for biodiesel. A protocol for in wk propagation of this plant was standardized using seedling explants. Growth regulators (GR) including gibberellic acid $(GA_3),\;N^6-benzylaminopurine(BA)$, thidiazuron (TDZ), and Adenine sulphate (Ads) were tested for optimum germination of seeds. Removal of seed coat prior to germination, controlled fungal growth partially but enhanced bacterial growth. Antibiotic cefotaxime was ineffective in controlling bacterial contamination. Seedling derived nodal explants and cotyledon nodes with attached cotyledons were excised and cultured for induction of shoots. Optimum sprouting and multiplication of shoot buds were obtained in MS medium supplemented with $8.88{\mu}M$ BA. These buds differentiated and rooted on medium devoid of GR. Optimum growth of Pongamia seedling was obtained in cotton plugged culture vessels. Reculturing of the cotyledon node explants produced more shoots from the same site. This process of removing shoots and reculturing of cotyledon node was followed for eight passages yielding 4 to 8 shoots in each cycle. The shoots (75%) rooted on half strength MS basal medium supplemented with 0.22% charcoal. All plants survived on transfer to soil. This is the first report on in vitro regeneration of Pongamia pinnata. This report demonstrates the possibility of coupling more than one parameter in single experiment to hasten the process of standardization. The process of cycling the nodal explant repeatedly for production of large number of shoots from single meristem may find application in genetic transformation experiments wherein meristems are used for transformation.

• 한국멀티미디어학회논문지
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• 제20권4호
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• pp.640-648
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• 2017

Sensors have limited resources in sensor networks, so efficient use of energy is important. In order to use the energy of the sensor node efficiently, researches applying mobile sink to the sensor network have been actively carried out. The sink node moves the sensor network, collects data from each sensor node, which spread the energy concentrated around the sink node, thereby extending the entire life cycle of the network. But, when the sink node moves, it requires a reset of the data transmission path, which causes a lot of control messages and delays. In this paper, we propose a CMS(Cluster-based Mobile Sink) method to support the movement of mobile sink in a cluster sensor environment. The proposed scheme minimizes an amount of control messages without resetting the routing paths of entire sensor networks by supporting the sink mobility path using the neighbor cluster list. And, it simplifies the routing path setup process by setting a single hop path between clusters without a gateway. The experiment results show that the proposed scheme has superior energy efficiency in processing and network structure, compared with existing clustering and mesh routing protocols.

• 한국멀티미디어학회논문지
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• 제24권3호
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• pp.431-439
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• 2021

Since the sensor nodes are randomly arranged in the region of interest, it may happen that the sensor network area is separated or there is no sensor node in some area. In addition, after the sensor nodes are deployed in the sensor network, a coverage hole may occur due to the exhaustion of energy or physical destruction of the sensor nodes. The coverage hole can greatly affect the overall performance of the sensor network, such as reducing the data reliability of the sensor network, changing the network topology, disconnecting the data link, and worsening the transmission load. Therefore, sensor network coverage hole recovery has been studied. Existing coverage hole recovery studies present very complex geometric methods and procedures in the two-step process of finding a coverage hole and recovering a coverage hole. This study proposes a method for discovering and recovering a coverage hole in a sensor network, discovering that the sensor node is a boundary node by itself, and determining the location of a mobile node to be added. The proposed method is expected to have better efficiency in terms of complexity and message transmission compared to previous methods.

• 한국작물학회지
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• 제49권1호
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• pp.69-72
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• 2004

Somaclonal variation, defined as phenotypic and genetic variations among regenerated plants from a parental plant, could be caused by changes in chromosome structure, single gene mutation, cytoplasm genetic mutation, insertion of transposable elements, and DNA methylation during plant regeneration. The objective of this study was to evaluate DNA variations among somaclonal variants from the cotyledonary node culture in soybean. A total of 61 soybean somaclones including seven $\textrm{R}_1$ lines and seven $\textrm{R}_2$ lines from Iksannamulkong as well as 27 $\textrm{R}_1$ lines and 20 $\textrm{R}_2$ lines from Jinju 1 were regenerated by organogenesis from the soybean cotyledonary node culture system. Field evaluation revealed no phenotypic difference in major agronomic traits between somaclonal variants and their wild types. AFLP and SSR analyses were performed to detect variations at the DNA level among somaclonal variants of two varieties. Based on AFLP analysis using 36 primer sets, 17 of 892 bands were polymorphic between Iksannamulkong and its somaclonal variants and 11 of 887 bands were polymorphic between Jinju 1 and its somaclonal variants, indicating the presence of DNA sequence change during plant regeneration. Using 36 SSR markers, two polymorphic SSR markers were detected between Iksannamulkong and its somaclonal variants. Sequence comparison amplified with the primers flanking Satt545 showed four additional stretches of ATT repeat in the variant. This suggests that variation at the DNA level between somaclonal variants and their wild types could provide basis for inducing mutation via plant regeneration and broadening crop genetic diversity.

• Journal of Plant Biotechnology
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• 제34권2호
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• pp.161-166
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• 2007

In vitro shoot regeneration and multiple shoot induction has been obtained from the stolen node explants in Eremochloa ophiuroides (Munro) Hack. The highest number of shoots ($10.66{\pm}0.21$) was observed from initial explants after one month culture duration on Murashige and Skoog (MS) medium containing 6-benzyladenine (BA: 0.5 mg/l). First generation shoot was excised and sub-cultured on the same fresh media for further multiplication of shoots. An enhanced number of second round shoots ($15.33{\pm}0.21$) was obtained compared to the initial culture media containing BA (0.5 mg/l). The number of shoots/stolon node was higher among all the concentrations of BA than kinetin (KN). In vitro regenerated shoots were successfully rooted in the phytohormone free MS medium. Plantlets generated with roots were transferred to pots containing compound mixture of soil and kept in green house conditions. Acclimatized plants showed 100% survival rate with normal morphology in green house conditions. The present study demonstrates the effect of explant and different plant growth regulators towards in vitro response in E. ophiuroides. Moreover, the study reveals the effect of cytokinin on induction of shoot number per stolen node explant in E. ophiuroides.

• 문화기술의 융합
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• 제8권2호
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• pp.379-384
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• 2022

무선 센서 네트워크에서는 직접 접근이 어려운 환경에 대량으로 센서 노드들이 배치된다. 배터리 교체나 재충전 등 전력 공급이 어렵다. 에너지를 센서 노드와 같이 사용하는 것이 매우 중요하다. 따라서, 네트워크의 수명을 늘리기 위해 중요한 고려 사항은 각 센서 노드의 에너지 소비를 최소화하는 것이다. 무선 센서 노드의 에너지가 에너지를 다하여 방전되면 센서 노드의 제 역할을 할 수 없으며, 네트워크 내 노드의 일정량(50% 또는 80%) 이상이 소진되면 네트워크가 제 역할을 하지 못한다. 따라서 노드의 에너지 소비를 최소화하고 네트워크를 장기간 유지하기 위해 다양한 프로토콜에서 제안된 방법이다. 우리는 클러스터의 중심점과 잔류 에너지를 고려하고 플롯 포인트와 K-평균을 고려한다(WSN은 최적의 클러스터링 클러스터링을 제안한다). KOCED 프로토콜에 대한 성능 평가를 하고자한다. 최근 머신러닝 방법 중 하나인 K-평균 알고리즘을 적용한 프로토콜을 비교하고 성능 평가 요소를 제시하고자 한다.

• 문화기술의 융합
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• 제8권6호
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• pp.531-536
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• 2022

전 세계적으로 국가경쟁력, 효용성, 중요성 등의 이유로 메가시티가 속속 등장하고 있으며, 점차 각 국가의 중심지로 자리를 잡아가고 있다. 또한, 메가시티는 현존, 잠재적, 초국가, 비군사 등 다양한 위협에 노출될 수밖에 없다. 특히, 최근 우크라이나-러시아 전쟁에서 볼 수 있듯이 국가기반체계는 적대 및 위협세력의 주요 목표가 되고 있다. 하지만 메가시티의 막대한 규모, 다양한 위협의 양상, 한정된 자원 등을 고려할 때 모든 국가기반체계를 완벽하게 방호하는 것은 현실적으로 어렵다. 따라서 여러 국가기반체계 중 핵심노드를 식별하여 중점적으로 방호하는 접근이 필요하다. 핵심노드는 다양한 국가기반시설이 연결된 국가기반체계의 중요도가 매우 높면서도 동시에 급소에 해당한다. 이를 위해 본 연구에서는 핵심노드를 논리적·체계적으로 식별하기 위해 네트워크 이론인 노드 중심성(Centrality) 분석방법과 군사이론인 중심(Center of Gravity) 분석방법을 제시하였다.