• Korean journal of applied entomology
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• v.47 no.4
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• pp.447-456
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• 2008

Five effective strains against tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae), Steinernema carpocapsae (GSN1), Steinernema sp. (GSNUS-10), Steinernema sp. (GSNUS-14), Heterorhabditis bacteriophora Hamyang (HbH), and Heterorhabditis sp. (GSNUH-1) were selected among 14 isolates of Korean entomopathogenic nematode in laboratory tests. $LC_{50}$ values of above five strains against tobacco cutworm were various by different nematode strains and developmental stages of tobacco cutworm. $LC_{50}$ value of S. carpocapsae (GSN1) was the lowest by $4.0{\sim}8.3$ infective juveniles (Ijs) and 2nd instars of tobacco cutworm was most susceptible. Pathogenicity of five effective strains against tobacco cutworm depends on nematode strain, concentration, and application times. The most effective strain was determined as S. carpocapsae (GSN1). Two or three times of applications were effective regardless of nematode strain, or concentration. Efficacy of S. carpocapsae (GSN1), Steinernema (GSNUS-10), Steinernema (GSNUS-14), and Heterorhabditis (GSNUH-1) was variable depending on nematode strain, concentration, application times, and host variety. S. carpocapsae (GSN1) was the most effective and inoculation of 100,000 infective juveniles per m2 (720,000 Ijs/7.2 $m^2=1{\times}10^9$ Ijs/ha) resulted in higher efficacy. Three times of application of nematodes led to higher control efficacy than one or two applications. Efficacy of nematodes was higher on Chinese cabbage than cabbage or kale.

• The Korean Journal of Pesticide Science
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• v.7 no.1
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• pp.18-24
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• 2003

The activities of esterase and acetylcholinesterase(AChE) on the Diamond backmoth (DBM), Plutella xylostella (Lepidoptera: Yponomeutidae) and Beet armywarm (BAW), Spodoptera exigua (Lepidoptera: Noctuidae) and inhibitions of AChE with flupyrazofos were clarified from the results of a series of experiments. These findings are described in brief as follows. The AChE activities of DBM and BAW in heads were $1.5{\sim}11.1{\mu}$mol/g/min in $1st{\sim}4th$ instar larvae of DBM and $1.7{\sim}45.2{\mu}$mol/g/min in $1st{\sim}6th$ instar larvae of BAW, respectively. Those were $25{\sim}30$ times higher in above 4th instar larvae of BAW than that of the 1st instar larvae of DBM. The activities of aliesterase in heads were $1.7{\sim}4.7$ times higher in $2nd{\sim}4th$ instar larvae of DBM and $8{\sim}55$ times higher in $3rd{\sim}6th$ instar larvae of BAW than 1st instar larvae of DBM. In abdomens, those were $3{\sim}17$ times higher in $2nd{\sim}4th$ instar larvae of DBM and $12{\sim}30$ times higher in $3rd{\sim}6th$ instar larvae of BAW than 1st instar larvae of DBM. Median AChE inhibition concentration $(I_{50})$ of flupyrazofos to the 2nd instar larvae of DBM and BAW were 92 nM and $15{\mu}M$, respectively, and those to the 4th instar larvae of DBM and BAW were $1.8{\mu}M$ and 3.1 mM, respectively. Insensitivity ratio of flupyrazofos in the 2nd instar BAW larvae showed ca. 162 times higher than that in the 2nd instar larvae of DBM, and that of the 4th instar BAW larvae showed ca. 1,720 times higher insensitivity to flupyrazofos than that of the 4th instar DBM larvae. AChE activities in the 2nd instar larvae of DBM and BAW at 32 h after applicaton of flupyrazofos decreased from 67.6% to 32.4% of the activity of the untreated control. That of the 4th instar larvae of DBM increased for 0.5 h after application flupyrazofos up to 75% of the untreated control, and after that it decreased to 34.5% of the untreated control at 32 h. In contrast, in the 4th instar larvae of BAW AChE activities increased for 8 h gradually up to 102 % of the activity of the untreated control, and then the activity decreased to 97% of the untreated control at 16 h after treatment.

• Korean journal of applied entomology
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• v.61 no.3
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• pp.423-434
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• 2022

The two previously developed artificial diets (N4 and N6) used for rearing Spodoptera frugiperda (Noctuidae) larvae, were selected as highly-fit ones for rearing Mythimna loreyi larvae. Almost all biological characteristics were not significantly different between the colonies reared on the two diets at 25℃ and 15:9 h (light:dark) photoperiod. The developmental periods were 4.9-5.2 days for eggs, and 22.3-23.2 days for larvae. The pupal period and weight were different between the sexes in each diet colony. The pupal periods in females and males showed 12.6-12.8 days and 14.1-14.5 days, respectively. The pupal weights were ca. 345 mg for females and ca. 380 mg for males. The pupation and emergence rates were ca. 91-94%, and ca. 91-95%, respectively, without significant differences between the two colonies. The pre-oviposition and oviposition periods were 3.4 days and 4.7-4.8 days, respectively. The adult longevity was 8.2 days in females and 10.3-12.4 days in males. Total offsprings produced were found to be 724-847 larvae on an average with ca. 1,400 maximum larvae. In the life table analysis, the intrinsic rates of increases (0.1181 for N4 and 0.1253 for N6) were not significantly different between the two colonies. Individual differences in the larval instar number 5 and 6 were found within a diet colony. The ratios of 5-instar larvae were ca. 22% in N4 colony and ca. 7% in N6 colony. The larval period of 6-instar larvae was longer than that of 5-instar larvae. Width of head capsule in larvae varied from ca. 309 ㎛ for 1st instar to ca. 3,065 ㎛ for 6th instar. Body lengths measured from ca. 2.0 mm for 1st instar to ca. 29.1 mm for 6th instar. Larvae of M. loreyi and M. separata were found at the same time in a maize field during June and July, 2020.

• Korean Journal of Environmental Biology
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• v.40 no.2
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• pp.187-198
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• 2022

In this study, Telenomus remus Nixon (Hymenoptera: Scelionidae) was first reported as a natural enemy of the fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) egg collected from corn fields in Korea, and its biological characteristics was studied. Based on morphological and molecular analysis, the parasitoid emerged from S. frugiperda eggs was identified as T. remus. We found that T. remus can attack the eggs of S. frugiperda, Spodoptera litra and Spodoptera exigua under a laboratory condition. The longevity of T. remus female adult was longer than that of male adult. The egg-to-adult period of T. remus was not affected by the host age and sex. T. remus female adult laid at least 1-3 eggs a day to a maximum of 37 or more eggs, and the most oviposited on the 3^rd to 4^th day after emergence. The host preference for oviposition of T. remus adult was high in the order of S. litura>S. exigua>S. frugiperda. T. remus preferred to parasitize 1- and 2-day-old host egg rather than 3-day-old host egg. When compared to the sex ratio of T. remus progeny, the rate of female progeny was higher at the initiation time of oviposition, while the proportion of male progeny increased significantly with female adult age, especially after 8-day-old adult. This information may be useful for improving T. remus mass rearing system and developing a biological control program to control S. frugiperda.

• Weed & Turfgrass Science
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• v.2 no.1
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• pp.62-69
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• 2013

The black cutworm, Agrotis ipsilon (Hufnagel), damages various cultivated crops and it can also be a serious pest of turfgrass, especially on golf courses. Essential oils have potential as alternative control agents for insect pests. Sixteen essential oils (anise, camphor, cinnamon, citronella, clove, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, thyme, turpentine and tea saponin) and paraffin oil were assessed in the laboratory, the green house and field trials for their efficacy against black cutworms in turf. Treatment of potted cores of perennial ryegrass turf with anise, cinnamon, neem, paraffin or turpentine reduced black cutworm damage in a greenhouse trial, and in a similar trial, applying neem oil at 4000, 2000 and 1000 ppm resulted in 100, 100 and 64% mortality, respectively, of black cutworms. Weight of survivors at the 1000 ppm rate was 5- fold less than weight of comparably-aged controls. Neem oil (2000 ppm) reduced growth of black cutworms feeding on treated clippings. A high rate of neem oil followed by irrigation (0.1 L of 20000 ppm neem oil with 0.9 L watering/$m^2$) was more effective than a lower concentration (1 L of 2000 ppm neem oil/$m^2$) against $2^{nd}$ and $3^{rd}$ instars in potted turf cores and field plots, respectively. However, not even the aforementioned higher rate effectively controlled $4^{th}$ instars in the field.

• Korean journal of applied entomology
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• v.52 no.4
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• pp.341-348
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• 2013

We desinged and evaluated a remote-sensing sex pheromone trap for real-time monitoring of Mythimna separata (Lepidoptera: Noctuidae), a migratory insect in Korea. The system consisted of a modified cone-trap with a sex pheromone lure, a sensing module based on light interruption, a signal transmission module based on code division multiple access, a main electronic board for system control, a power supply based on a solar collector, a stainless steel-pole supporting the system, and a signal collection and display system based on an internet web page. The ratio (>92%) of the actual number of insects to the signal number in the remote-sensing trap was improved by sensing only within a limited period at night on the basis of the insect's circadian rhythm, control of signal sensitivity on the basis of sensing software programming, 1-h interval for signal transmission, and adjustment of the signal transmission program. The signal occurrence pattern in the remote-sensing trap was conclusively similar (correlation coefficient, >0.98) to the actual pattern of adult occurrence in the trap. The result indicated that the remote-sensing trap based on the attraction of the sex pheromone lure for M. separata has a promising potential for practical use. Occurrence of M. separata adults was observed several times in 2011 and 2012, and the peaks were sharp.

• Korean journal of applied entomology
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• v.42 no.3
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• pp.225-231
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• 2003

The susceptibility of the different larval stages of Spodoptera litura to nine insecticides was evaluated using the perilla leaf-dipping method. Median lethal concentration ($LC_{50}$) was increased with larval development in the range of 0.5 ppm to 5.6 ppm, 9.9 ppm to 27.9 ppm, 9.6 ppm to 125.1 ppm and 24.3 ppm to 546.6 ppm in the 1st, 2nd, 3rd and 4th instar, respectively. The tolerance ratio (TR), which is the TR of 90 percent lethal concentration (LC/ sub 90/) to the recommended concentration, was 0.04 to 0.8 in the 1 st, 0.2 to 7.5 in the 2nd, 0.7 to 115.3 in the 3rd and 1.2 to 485.4 in the 4th instars. Lower D$LC_{50}$ and DTR, which is the difference between the $LC_{50}$ and the TR of 4th and other instars, respectively, were observed in chlorfenapyr, chlorpyrifos and EPN while higher ones were lufenuron, chlorfluazuron and teflubenzuron. These results mean that insecticides with lower D$LC_{50}$ and DTR are effective in controlling larva of S. litura collected in Milyang, Korea.

• Korean journal of applied entomology
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• v.43 no.2
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• pp.175-180
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• 2004

This study was conducted to determine the effect of sublethal concentrations ($LC_{10}$ and $LC_{30}$) of insecticides on pupal duration, emergence, adult longevity and oviposition of tobacco cutworm, Spodoptera litura Fabricius, when 3rd instar larva of tobacco cutworm was treated with insecticides of chlorpyrifos, ethofenprox, chlorfenapyr-bifenthrin and hexaflumuron-chlorpyrifos . Pupal duration of female and male were 6.9 days and 8.0 days at untreatment, and were 7.2 to 7.6 days and 8.3 to 8.6 days at insecticide treatment, respectively. Thus pupal duration at the insecticide treatment was slightly longer than that at the untreatment, and that of the males was slightly longer than that of the females even though significant difference between sublethal concentrations and among insecticides was not observed. Percent mergence was 88％ at untreatment and ranged from 79％ to 95％, in particular which showed above 91％ treated with chlorfenapyr-bifenthrin and ethofenprox, at insecticide treatment. Adult longevity was 7.7 days and 7.9 days for female and male at untreatment respectively, and 7.1 to 8.4 days for female and 7.7 days to 9.0 days for male at treatment. There was a significant difference between insecticides and sublethal concentrations of insecticides except showed the longest adult longevity at hexaflumuron-chlolfyrifos treatment. Total number of eggs laid were less at treatment (778-948) than that (1,010) at untreatment regardless of sublethal concentrations of insecticides. Accordingly the pupal duration and oviposition of tobacco cutworm were affected at the insecticide treatment of sublethal concentration.

• Korean Journal of Environmental Biology
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• v.35 no.1
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• pp.47-56
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• 2017

This study investigates the spatial and temporal variations of moth diversity of Bulgapsan Mountain, Younggwang, Jeonnam. Moth collecting was carried out using a UV light trap at three different sites from February to October 2016 (5 times). A total of 177 moth species and 911 individuals in 17 families were identified. The family Geometridae (51 species and 282 individuals) was the most dominant in the numbers of species and individuals, followed by Erebidae (46 species, 248 individuals), Noctuidae (34 species, 219 individuals) and others. Seasonal change of the number of species and individuals showed a M-shaped pattern with May and July peaks. The Chao 1 estimated number of species using the observed species was 224~326 species, but the graph did not reach an asymptote. Among the surveyed sites, the Neolitsea sericea community showed the highest numbers of species and individuals.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.849-857
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• 2016

In the growth of the Rubus coreanus Miquel fruits, the unripened (10 days) and ripened (25 days) fruits after flowering were harvested. The chemical characteristics of different maturational stages of the unripened and ripened fruits were investigated. Total amount of phenolic compounds was 4.00-7.56% in the unripened fruits and 3.78-5.57% in the ripened fruits, respectively. Furthermore, total amounts of organic acids such as malic, citric, succinic, and oxalic acids were 16.40 mg/100 g in the unripened fruits and 28.82 mg/100 g in the ripened fruits, respectively. In organic acids of the unripened and the ripened fruits, citric acid (8.76-15.47 mg/100 g) was the highest amount among other organic acids. Soluble sugars were significantly increased from 11.07 to 21.54% in the unripened and ripened fruits. Therefore, ripened fruits had the high levels of phenolic compounds, organic acids and soluble sugars. For the biological studies of R. coreanus, methanol extracts of R. coreanus leaves, fruits, and stems were evaluated for their insecticidal activities against Myzus persicae (Hemiptera: Aphididae), Plutella xylostella (Lepioptera: Plutellidae) and Spodoptera litura (Lepioptera: Noctuidae) by leaf dipping method. From these results, the extract of R. coreanus leaves revealed potent insecticidal activity against P. xylostella. However, the methanol extracts of R. coreanus fruits and stems had no any insecticidal activity against M. persicae, P. xylostella and S. litura. The R. coreanus leaves have promising potential as new insecticidal agent against P. xylostella.