• 제목/요약/키워드: No-washing

검색결과 449건 처리시간 0.026초

부산 배산성지 1호 집수지 출토 대나무 발 수습 및 보존처리 (Conservation Treatment on the Bamboo Sunblind from the No. 1 Catchment Site in Baesanseongji, Busan)

  • 박지현;박정혜;이광희;서연주;박정욱;김수철
    • 보존과학회지
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    • 제37권5호
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    • pp.536-544
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    • 2021
  • 배산성지 1호 집수지 내 출토 대나무 발의 안정적인 보존처리 수행으로 향후 안전한 관리와 가치 향상을 꾀하였다. 다양한 분석결과 발의 제작에 사용된 주재료는 대나무였으며, 초본류를 사용하여 대나무를 엮고 두 재료 간의 접착을 위해서 옻칠을 한 것으로 판단되었다. 블록 형태로 수습한 대나무 발은 세척 동안 흐트러지지 않도록 임시 석고틀을 제작, 결구하여 고착된 오염물과 흙을 모두 제거하고 개별로 분리하여 세척하였다. 이후 강화처리를 위해 PEG 함침법을 적용하였다. 예비실험결과를 바탕으로 건조과정 중 발생할 수 있는 유물의 손상을 방지하기 위해 스테인리스 고정틀로 형태를 고정한 후 진공동결건조를 실시하였다. 유물의 표면안정화를 위한 표면처리제는 PEG 20%(In Ethyl Alcohol)를 적용하였다. 표면처리 후 대나무발은 최대 길이에 맞춰 편을 접합하고 교란층과 같은 미상부재를 최대한 활용하여 결실부를 채워 배접방식으로 발의 형태를 최대한 복원하였다. 배접된 대나무발은 제작한 틀에 고정하여 보존처리를 완료하였다.

동결(凍結)과 해빙(解氷) 기간(期間)중 토양내(土壤內) 양분(養分) 용질(溶質)의 이동(移動) (Nutrient Solute Transport during the Course of Freezing and Thawing of Soils in Korea)

  • 하상건;정영상;임형식
    • 한국토양비료학회지
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    • 제28권2호
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    • pp.135-144
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    • 1995
  • 겨울 동안에 일어나는 동결(凍結)과 해빙(解氷)과정에서 토양(土壤)내 용질의 이동(移動)양상을 구명하기 위하여 실내시험과 포장시험을 수행하였다. 실내 시험은 직경 5cm, 두께 0.2cm, 길이 1cm인 아크릴관을 30cm 길이로 연결하여 단열재로 싸 토주(土柱)를 제작하였다 상부 5cm 까지는 $KNO_3$를 20mmol/kg 수준으로 처리하여 수분함량(水分含量)을 조절하였다. 상부는 $-6{\sim}-8^{\circ}C$로 유지된 냉동실(冷凍室)에 그리고 하면은 $0.5{\sim}3.5^{\circ}C$로 유지된 냉장실(冷藏室)에 연결하여 토양(土壤)의 깊이 5cm까지 동결(凍結)시킨 후 꺼내어 1cm씩 절단한 뒤 $NO_3^-$, $NH_4^+$$K^+$의 함량(含量)을 분석하였다. 포장시험은 해발표고 840m인 대관령(大關嶺) 고령지시험장 포장과 해발고도 74m인 춘천(春川) 강원도농촌진흥원 포장에 직경 20cm, 길이 1m되는 토주(土柱)를 12월에 묻고 20cm까지 $KNO_3$를 50mmol/kg 수준으로 처리하여 동결(凍結)이 진행되는 기간과 해빙(解氷)된 후에 토양(土壤)시료를 채취하여 분석하였다. 실내 시험결과 동결(凍結)이 진행되면서 $NO_3^-$가 표면 부근으로 상승(上昇)하는 것이 확인 되었으며, 이는 동결(凍結)진행에 따른 수분(水分) 이동(移動)의 결과로 판단되었다. $K^+$도 표면 부근으로 상승(上昇)하였는데, 그 양은 $NO_3^-$의 1/5~1/10이었다. 포장시험결과 동결(凍結)진행에 따라서 실내시험과 비슷하게 표토로 상승(上昇)하는 것이 확인되었으며, 봄에 해동되면서 많은 양의 질소(窒素)가 손실(損失)되었다. 처리부위 20cm 이하로 용설(溶說)된 양은 처리된 질소(窒素)의 17~24%이었으며, 50cm 이하로 내려가지는 않았다. 용탈(溶脫)된 양을 제외한 토양단면(土壤斷面)에서 순 손실(損失)량은 8.7~39.5%로, 온도(溫度)가 낮고 눈이 많은 대관령(大關嶺)에서 손실량(損失量)이 춘천(春川)에서보다 많았으며, 투수속도가 느린 식양토(埴壤土)에서 미사질양토(微砂質壤土)보다 많았다. 이 같은 사실로 미루어 보아 겨울 동결(凍結)기간 중 질소(窒素)의 손실(損失)은 탈질(脫窒)과 용탈(溶脫) 이외에 해빙기(解氷期)에 표면에서부터 녹은 물의 지표유실에 따를 세탈(洗脫)에 의한 부분이 많은 것으로 판단되었다.

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수도(水稻)에 처리(處理)된 유기수은제(有機水銀劑)의 잔류성(殘留性)에 관(關)한 연구(硏究) -제1보(第1報) 침지용유기수은제(浸漬用有機水銀劑)로 처리(處理)된 수도종자중(水稻種子中)의 수은잔류량(水銀殘留量)에 관(關)하여- (Studies on the Organo-mercury Residues in Rice Grain -I. Mercury residues in rice seeds treated with organo-mercury fungicide-)

  • 이동석
    • Applied Biological Chemistry
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    • 제8권
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    • pp.87-93
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    • 1967
  • acaricide로 알려진 phthalimidomethyl O,O-dimethyl phosphorodithioat (Imidan)을 수도(水稻)에 살포(撒布)했을 때 Imidan과 그 대사물질(代謝物質)이 식물(植物)의 생육(生育)에 미치는 영향(影響)을 연구(硏究)하기 위하여 본(本) 실험(實驗)을 하였으며 이의 결과(結果)를 요약(要約)해 보면 다음과 같다. (1) Imian의 대사물질(代謝物質)로 예상(豫想)되는 다음의 8가지 화합물(化合物)을 합성(合成) 또는 정제(精製)하여 공시약제(供試藥劑)로 사용(使用)하였다. (a) N-Hydroxy methyl phthalimide (b) Phalimide (c) Phthalamdic acid (d) Phthalic acid (e) Anthranilic acid (f) p-amino benzoic acid (g) p-hydroxu benzoic acid (h) Benzoic acid (2) 상기(上記) 물질중(物質中)에서 (a),(c),(d),(e)와 Imidan의 각(各) 10 ppm과 20 ppm 의 Buffer Solution 을 만들어 밀 종자(種子)를 가지고 coleoptile straight growth test를 해 본 결과(結果) Imidan 은 10 ppm 과 20 ppm에서 모두 control 보다 생장(生長)의 촉진효과(促進效果)를 보였으며 기중(其中) phthalamidic acid 10 ppm 이 가장 좋은 성적(成績)을 보였다. 이것으로 보아 Imidan 자체(自體)는 생장(生長) 억제(抑制)의 효과(效果)를f 보이나 이것이 일단(一但) 생체내(生體內)에서 가수분해(加水分解)를 비롯한 각종(各種) 대사작용(代謝作用)을 받으면 그 대사산물(代謝産物)이 식물생장(植物生長)을 촉진(促進)하는 효과(效果)를 보이는 것 같다. (Table 1, Fig. 1 참조(參照)) (3) xylene을 용매(溶媒)로 하여 Imidan 유제(乳劑)를 만들고 이것을 희석(稀釋)하여 20 ppm, 100 ppm 및 200 ppm 의 각(各) 농도(濃度) 유화액(乳化液)을 조제(調製)한 후 이것을 배지(培地)로 하여 수도종자(水稻種子)를 발아(發芽)시킨 후 12 일(日)에 shoot와 root의 길이를 측정(測定)하였다. 이의 결과(結果)를 보면 root는 Imidan 20 ppm에서, shoot 는 Imidan 100 ppm에서 모두 xylene만의 유제구(乳劑區)인 control 보다 좋은 효과(效果)를 보였으며 여기에서 흥미(興味)있는 것은 용매(溶媒)로 사용(使用)된 xylene은 수도종자(水稻種子) 뿌리의 발육(發育)에 심(甚)한 억제효과(抑制效果)를 보이는 것 같다. (Table 2, Table 5 참조(參照)) (4) 벼를 pot에 심고 2회(回)에 걸쳐 control, Imidan, N-hydroxy methyl phthalimide, anthranilic acid 및 phthalimide의 10, 25, 50, 100 ppm 농도(濃度)의 각(各) 유제(乳劑)를 살포하고 일정기간후(一定期間後) 생육상(生育相)을 조사(調査)하였더니 Imidan 구(區)와 N-hydroxy methyl phthalimide 구(區)가 control 보다 좋은 성적(成績)을 보였다. (5) Imidan 250 ppm 유제(乳劑)를 수도엽면(水稻葉面)에 살포(撒布)하고 3 일(日), 5 일(日), 7 일(日) 및 14일후(日後)에 일정량(一定量)의 엽경(葉莖)을 채취(採取)하여 acetone으로 추출(抽出)k고 acetonitrile을 가지고 prechromatographic piriication 을 거쳐 paper chromatography에 의(依)하여 다음과 같은 대사물질(代謝物質)을 검출(檢出)하였다. Imidan $(Rf:\;0.97{\sim}0.98)$, N-hydroxy methyl phthalimide (Rf: 0.87), phthalimide $(Rf:\;0.86{\sim}0.87)$, phthalamidic acid $(Rf:\;0.13{\sim}0.14)$, phthalic acid $(Rf:\;0.02{\sim}0.03)$, benzoic acid $(Rf:\;0.42{\sim}0.43)$ 및 p-amino benzoic acid 또는 p-hydroxy benzoic acid $(Rf:\;0.08{\sim}0.09)$와 Rf=0.73, 0.59, 0.33, 0.23, 0.07의 미지물질(未知物質)을 검출(檢出)하였다. 또한 3일(日), 5일(日) 등(等) 초기(初期)에서는 미분해(未分解)의 Imidan과 최초(最初)의 가수분해(加水分解) 산물(産物)인 N-hydroxy methyl phthalimide등(等)이 비교적(比較的) 다량(多量)으로 검출(檢出)되었으나 7일(日), 14일(日) 등(等) 후기(後期)에는 생체내(生體內)에서 더 많은 분해(分解)를 받아 상기(上記) 이성분(二成分)은 양(量)이 감소(減少)되고 phthalic acid, phthalamidic acid benzoic acid 및 p-hydroxy benzoic acid 또는 p-amino benzoic acid등(等)의 양(量)이 증가(增加)되는 것을 볼 수있었으며 도체상(稻體上)에 살포(撒布)된 Imidan 은 체내(體內)에 흡수(吸收)되어 14일(日)이 경과(經過)되면 대부분(大部分)이 분해(分解)를 받는 것으로 보여진다. 이상(以上)의 결과(結果)로 보아 Imidan은 자체(自體)로서는 식물생장(植物生長) 촉진작용(促進作用)이 없으나 식물체내(植物體內)에서 여러 가지 대사작용(代謝作用)(enzyme의 작용(作用))을 맡아서 각종(各種) phthaloyl 영향(影響)을 주는 것으로 생각(生覺)된다.

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초등학교 아동의 개인위생에 대한 조사 (A Study on Personal hygiene of Primary School Students)

  • 김재삼;남철현;강희양
    • 한국학교보건학회지
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    • 제9권1호
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    • pp.109-123
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    • 1996
  • The study was designed to gain necessary basic data, in order to grasp the actual condition on personal hygiene of primary school students and to help school health education and child health education data development of parents of students, the survery was carried out through this reporter's interview for mothers who have primary school pupil in KyungBug area during the period of a month from 14 the April to 30 the April 1994. The results of this study can be summarized as follows. 1. As for general characteristics, the percent of answer mothers with 30~34 years 44.9%, and the percent of answer mothers with 35~39 years was 37.6%, 14.6% of answer mother was 40 years and over. The most house types was apartment house, that is 64.8% and independence house was 18.9%, 13.7% of answer mothers was multivalent house residents. As for level of school career, the percent of high school was 64.2% and the percent of university was 20.5%, 10.1% of answer mothers was finishment of middle school. As for religion a lack of religion was 38.4% and a Buddhist was 36.8%, a christian was 13.4%, 10.4% of answer mothers was a catholic. As for jobs, the percent of office was 33.9%, specialist and expert skiller was 17.3%, self-management was 16.0%, fishing and agrarian villiages was 11.6%, public service personnel and shool personnel was 9.1%. As for the number of sons and daughters, the percent of two was 70.5%, the percent of one was 16.0%, and the percent of male students was 54.1%. As for level of economic life middle level was 59.9%. 2. The percentage of washing hand after school hours was 66.8% and not washing hand after school hours was 33.2%. In washing hand after school hours family of independence house, mothers that have schoolcareer of university, female students, three and over of sons and daughters was high individually (p<0.05). 3. As for paring one's nails, once a ten days was 52.9% once a five days was 22.5% once a fifteen days was 19.0%, once a twenty and over was 5.6%. 4. 54.7% of primary school students of answer mother's take a bath once a few days and 31.6% take a bath once a week, 10.1% take a bath once a tendays. 5. The percentage of changing of underwear once a day was 60.9%, once a few days was 37.1%, once a week was 2.0%, as for changing of underwear, sons and daughters that has mothers with 29 years and downward, one number of sons and daughters, females students was high individually (p<0.01). 6. The percentage of haircut once a 20~30 days was 59.9%, once a 31~40 days was 17.9%, once a 40 days and over was 16.6%. As for the percentage of haircut once a 20~30 days apartment house residents, male students was high (p<0.01). 7. The percentage of experience in taking nutrition was 79.8% and the percentage of experience in taking restorative was 72.3%. As for taking nutrition, apartment house residents, one number of sons and daughters, male students was high individually (p<0.01). As for taking restorative apartment house residents, on number of sons and daughters, male students was high individually (p<0.05). 8. The percentage of habit of unbalanced diet was 44.0%, sons and daughters that have mother with 40 years and over apartment house residents, male students, one number of sons and daughters was high indivdually (p<0.05). 9. As for hygiene condition of sons and daughters, the percentage of good state of health was 65.2%, middle state of health was 3.5% bad state of health was 11.4%. In good state of health sons and daughters that have mothers with 29 years and downward, multivalent house residents, three numbers of sons and daughters, female students, high birth was high individually. 10. As for fattness of sons and daughters, existence was 18.2%. No existence was 81.8%. in existence sons and daughters who have mothers with 40 years and downward, independence house resident, special job and expert skill job, three and over number of sons and daughters, female students, low birth was high individually. 11. As for use aspect of medical facilities of sons and daughters, hospital doctors was 53.1%, drugstore was 42.3%, chinese medicine hospitals or health organization was 4.6%. In usage of drugstore, sons and daughters of mothers with 29 years, 40 years and over was 55.6%, 61.4% individully, inusage of hospital doctors 30~34 years, 34~39 tears was 64.5%, 49.1% individully (p<0.01), apartment house residents, one or two numbers of sons and daughters, male students was high individually. In the percentage of using drugstore, school career of middle school and downward, in occupation, three and over numbers of sons and daughters, low birth was high individually (p<0.05). According to the results mentioned above. An actual condition and a related matters on personal hygiene of primary school students must be used as the basis data of a health education program and a health data of a health education program and a health data related, a teaching materials development and must be helped to the health life education of parents of students and childs.

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한국재래산양에서 계절이 정소기능, 정자의 내동성 및 수정능력에 미치는 영향 I. 정액성상과 정자의 내동성 및 난자침입능력의 계절적 변화 (Effect of Season on Testis Function and Freezing and Fertilizing Ability of Spermatozoa in Korean Native Goat I. Seasonal Changes in Semen Characteristics and Freezing and Penetrating Ability of Sperm)

  • 김창근;정영채;김광식;윤종택;이장희;정영호;최선호;김흥률;김수;권처진
    • 한국가축번식학회지
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    • 제17권4호
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    • pp.311-323
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    • 1994
  • This study was conducted to observe seasonal and individual changes in semen characteristics and sperm freezability, and sperm penetration into zona-free hamster eggs in Korean native goats. Buck response and change in semen characteristics to electrical stimulations was evaluated for four seasons throughout 2 years and percentage of motile sperm and normal apical ridge acrosome was investigated after equilibration and thawing for 4 seasons with 5 bucks. Sperm penetration rate was evaluated for 4 bucks. 1. Probe insertion at depth of 7cm and repeated stimulation for 3 sec was more effective(P<0.05) in buck response and semen collection than those of other conditions. 2. Semen characteristics from electrojaculation was signficantly(P<0.005) higher in spring and fall for semen volume, in spring and summer for sperm concentration and in fall for sperm motility than those in other seasons, respectively. However, there were no differences in total sperm among seasons. 3. Buck response to electrical stimulation showed significant difference(P<0.05) among individuals in all 3 seasons except winter. Significant individual difference in semen volume was only in spring and summer, but there was no indivudual difference in sperm concentration and total sperm in all season. 4. Washing of semen before freezing treatment was greatly(P<0.05) beneficial to sperm motility after thawing, no matter whether ejaculates exhibit egg yolk coagulation or not. 5. Sperm motility after glycerol equilibration was significantly(P<0.05) low in summer semen and motility after thawing was greatly(P<0.05) higher in winter semen than in other seasons. Freezability of unwashed sperm was significantly difference among bucks, but a yearly freezability of washed sperm after chilling and thawing were no differences among bucks and percentage of normal apical ridge acrosome were not different among seasons and bucks. 6. There was no significant difference in sperm motility after thawing between egg yolk levels in summer, although 20% level gave more higher motility than 5% level. 7. In summer, 3.2% glycerol and 3-h equilibration gave greatest percentage(P<0.05) of sperm motility and normal apical ridge acrosome after thawing. 8. Sperm penetration rate into zona-free hamster eggs was not different between bucks and seasons. Overall, it is concluded that to obtain maximum sperm output and successive semen freezing by electrojaculation method, buck selection with good response in all season could be basically considered and that seasonal effect on sperm freezability was more greater than that of individual bucks.

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The Recognition about Food Wastes Treatment at Yongin Area

  • Kim, Jeong-Hyun;Kang, Hee-Joo;Kim, Kyung-Tae;Kim, Hyun-Soo;Hwang, Seong-Hee;Kim, Yong-Chul;Kim, Pan-Gyi
    • 한국환경보건학회지
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    • 제30권4호
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    • pp.329-343
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    • 2004
  • We conducted to investigate citizen's attitude to the treatment of food waste in Yongin city. The respondent of $41.81\%$ throws the food waste eliminate from home and store less than 1l in Yongin. When they dump the food waste, they speak out the inconvenience of damaged envelope by animals in case of home and store. This caused troubles for reason of sanitary. So a local autonomous entity must carefully consider of expand use specially designed container as like apartment house. They give an answer that the collecting time of food waste is suitable form dawn till morning. This answer shows the satisfaction of the period time to collect the food waste. They prefer to be appointed the exclusive place to collect food waste. The service interval of collect is suitable 1 time a day. They want to increase the number of washing of the collecting container. This is good method for sanitary condition, but the care of the period time to collect the food waste is more efficient than the care of the number of washing the collecting container. The care of the period time minimizes to incur the enmity of the people and to pollute in environment. The major of respondent handled the food waste after keeping the basket or a kit. This fact shows to us almost citizen doesn't feel the seriousness to remove the moisture of the food waste. Recently, many solutions which can be disposal efficiently are getting magnified and improved owing to increase utilities channel to loss in quantities and dry the food waste. We expect the reduction of food waste is solved getting easily step by step. The results of the awareness about the facility of food waste show citizen prefer recycling facility to the other facilities. If recycle facility and incineration facility are constructed, they were worried about bed smell. When some facility of the food waste is constructed, they have to maintain and to handle not to incur the enmity of the people. The spread rate of specially designed container already increased, the citizen set a high value on the use of specially designed container more than amount-rate vinyl envelope that people have used for several years. In the cost treatment about food waste, the major respondent answered the use cost of specially designed container is suitable price. So we can know the use charge is proper level. The majority of citizen more prefer autonomous plan which voluntary atmosphere creation and public information by mass media than levy system and rising treatment cost which forced plan. The citizens have pretty positive thinking of incineration, so the government needs more efforts for a public notice, which includes the incineration is no more than abandoned thing. Each of local self government has to sort the food waste and make kind of resource system related to collecting and carrying, constructing a suitable facility, proper disposal of the food waste and producing harmless in our surrounding in order to solve the invisible problems. To do above mentioned things, we have to analyze referred several problems till now. Also, to minimize the side effect, the government will have to improve through enforce the system.

일부도시국민학교취학아동의 보건생활에 관한 실태조사연구 (A Study on Health Aspects of Daily Life of Elementary School Children in an Urban Area)

  • 구외행
    • 대한간호학회지
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    • 제3권3호
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    • pp.36-49
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    • 1973
  • This study was carried out for the objectives to collect the basic informations on the health behaviors of the elementary school children in an urban area in Korea. Seven hundred students were drawn to fill in the designed questionnaire which carries variety of Questions on health re-lated behaviors in general, eating habits, disease history, mental health, and sex education. Questionnaire were filled in by their parents. Major findings are as follows: ① 55.7% had habits of washing the hands before eating whereas 59.8% trashing their hands after toilet. The others had no idea of washing hands before eating and after toilet. ② 26,5% had habits of brushing the teeth twice a day 54.7% only once in the morning, and 2.6% once only in the evening. Thus, the idea of prevention from decayed teeth seems to be lacking among the school children. ③ Bathing habits were also inquired to get 40.3% of bathing more than once a week, 43.1% once every two weeks, and the rest of 16.6% once every one to three months. ④ 41.7% keep the regular bedding time whereas 58.3% irregular. Physical exercises were con-ducted by 76.6% on the ground while 23.5% did not practice any physical exercises at all. Of those physical exercises, rope skipping occupied 37.5%, and the other 66.9% consisted of 14 different kinds of individual type physical exercises such as gymnastic exercise. The main reasons for not enjoying exercises were different by sex; boys largely complained the inadequacy and lack of gymnastic facilities and girls felt in short of friends who could join the exercises. ⑤ 31.9% of the school children had been taking not much of food while 28.3% had unbalanced diets. Of these unbalanced diets, meat occupied 33.2% to be the priority to have an order of the following items such as vegetables, bread or noodle, and fishes as next to each. For eating habits, 88.5% take simple snack such as bread (38.4%, cookies, fruits, and candies in order. 25.8% of the children were provided such snacks or their parents regularly. Breakfast was sufficiently taken by 45.0% whereas 8.4% had never sufficiently. As to the lunch, 63.6% had sufficiently while 16.8% insufficiently. 70.6% take breakfast with all family members together and 30.4% separately. Correlation of sufficient taking of breakfast and eating together of tile family member's seems to be significant when we compare 72.5% of sufficient takers who enjoy breakfast together with the family members with 55.6% of insufficient takers who enjoy it with the family. This finding allows the investigator to point out the importance of table circumstances for children's eating. ⑥ The most common disease was catching a cold (38.8%), and the second was stomach trouble to be followed by the frequency of car sickness, headache, and skin infection. Doctors are consulted only by 23.9% when they are sick whereas 59.7% resorted to the drug stores. The lower the educational attainment of the parents, the lower the rate of visiting clinics. ⑦ 36.7% of their parents pointed out the problems of personality guidance as the most difficult thing at home 71.3% of their parents worried about and unsatisfied with their children's personality traits. Of these complains of the parents, impatience stood at the top to be tabulated at 24.1%, and 21.1% indicated narrow-mindedness. In line with this primary socialization at home, the most crucial problem seems to be related with the lack or recognition of the parents'own role when we find only 43.1% of the parents understood the importance of their own role for the home education of children; the latter group attributed tile responsibility of personality formation to the children themselves. ⑧ As to the sex educational aspects, 30.9% of children have ever asked about the physiology of reproduction or sexual matters to their parents, of those parents only 17.0% could give the constructive responses to the inquiries of the children. In companies on with these data, 25.6% recognized their own role in sex education for their own children while the large segments of the parents (51.1%) attributed the responsibility of sex education to tile low level of 38.3% who recognized the importance of sex education in the school curriculum and 25.1% of the parents insisted to wait until they get to know naturally about sex. 38.1% of the parents said they had some knowledge on sex from books while 16.9% through mass media. The next groups had common senses of sex from their own parents, school friends and other sources.

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$V_H$ Gene Expression and its Regulation on Several Different B Cell Population by using in situ Hybridization technique

  • Jeong, Hyun-Do
    • 한국어병학회지
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    • 제6권2호
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    • pp.111-122
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    • 1993
  • B 세포의 $V_H$ 유전자가 어떠한 기작으로 선택되어지는 지는 현재 명확히 밝혀져 있지 않다. 본 연구에서는 transformation 등의 방법에 의한 편향된 분석결과를 피하고자 in situ hybridization 기법을 이용하여 정상적인 single 세포가 발현한 $V_H$ 유전자를 분석하였다. $V_H$ 유전자간에 나타나는 DNA 배열의 유사성 때문에 in situ 기법에서 가장 중요한 것은 probe 농도와 세척 stringency의 결정이다. LPS-stimulated된 spleen B 세포에 대해서 $C{\mu}$$V_HJ558$ $^{35}S$-RNA probe는 $2{\sim}4{\times}106cpm$/slide의 농도에서 낮은 background와 적정수의 positive 세포를 관찰할 수 있었으며 세척조건으로서는 $54^{\circ}C$에서 40~50%의 formamide를 사용할때 최적이라는 것을 $C{\mu}$, $V_{H}S107$, 그리고 $V_{H}J558$ probe를 이용한 실험에서 결정하였다. 위의 조건하에서 spleen B 세포가 발현한 $V_H$ 유전자를 분석하여 본 결과 각각의 $V_H$ gene family 발현 빈도는 각각의 family 크기에 비례하여 결정된다는 것을 알 수 있었다. 이러한 결과들은 여러 다른 발달 단계에 있는 bone marrow B 세포에 대해서도 동일한 결과를 보여 주어 어떤 특수 $V_H$ gene family의 발현이 B 세포의 발달단계에 따라 특이하게 변화하는 것은 아니라는 것을 나타내 보여 주었다. 그러므로 $V_H$ 유전자의 이용은 B 세포가 differentiation하는 것과는 무관하게 무작위 적으로 선택되어 진다는 것을 밝혔다.

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재배환경, 품종 및 가공 방법에 따른 고추와 고춧잎 중 농약의 잔류 특성 (Residual Characteristics of some Pesticides in/on Pepper Fruits and Leaves by Different Types, Growing and Processing Conditions)

  • 이희동;유오종;임양빈;권혜영;진용덕;김진배;김윤한;박승순;오경석;고성림;김태화;노재관;경기성
    • 농약과학회지
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    • 제10권2호
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    • pp.99-106
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    • 2006
  • 노지와 시설하우스에 재배중인 고추에 살포한 chlorothalonil과 imidaclopid는 시설하우스보다 노지에서 분해가 빨랐는데 시험기간 중 강우가 없었던 점을 고려하면 이는 주로 이슬에 의한 세척과 광분해에 의한 영향으로 추측되었다. 고추품종별 농약 잔류량 변화는 일정한 경향을 보이지 않았는데 이는 고추가 연속수확작물이고 또한 고추가 고춧잎 속에서 생육하여 농약이 골고루 묻지 않았기 때문으로 판단되었다. 풋고추를 절였을 경우에는 농약에 따라 약 $30{\sim}71%$의 잔류량 경감효과가 있었으며, 꽈리고추를 볶았을 경우에는 $20{\sim}41%$의 경감효과가 있었다. 또한 esfenvalerate와 imidacloprid를 살포한 홍고추를 이용하여 고추장을 담가 2개월간 발효시켰을 경우에는 고추장에서 농약이 검출되지 않았다. 수확한 고춧잎 중 잔류농약은 물세척에 의해 $22{\sim}37%$, 데침에 의해 $74{\sim}95%$, 데침 후 건조에 의해 $17{\sim}55%$의 고춧잎 중 잔류농약 경감율을 보였다.

선식용 곡류원료 및 제조공정에 따른 유해미생물 오염도 분석 (Analysis on Hazard Microorganisms in Raw Materials and Processing Environment for Sunsik Manufacture)

  • 김진희;이유근;양지영
    • 한국식품위생안전성학회지
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    • 제26권4호
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    • pp.410-416
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    • 2011
  • 선식의 품질향상과 위생적인 선식제조에 기초단계인 곡류원료에 위생 상태를 파악하기 위해 선식업체 19곳을 방문하여 선식용 곡류원료 10종류에 대한 일반생균수는 찹쌀 4.8~7.2, 보리 4.8~8.6, 현미 4.6~7.4, 검정콩 4.3~7.1, 검정쌀 4.1~7.1, 검정깨 4.1~6.1, 찰수수 3.1~5.1, 찰조 4.2~5.1, 들깨 3.1~5.1, 율무 3.6~5.2 log(CFU/g)로 검출되었고, Coliform, E. coli은 찹쌀 4.4~2.1, 2.1~3.4, 보리 2.2~4.4, 2.1~3.1, 현미 3.1~3.2, 1.0~2.8, 검정콩 2.3~3.8, 검정쌀 2.1~3.1, 1.0~2.1, 검정깨 2.3~3.6, 찰수수 2.2~2.4, 찰조 2.3~3.2, 들깨 2.3~3.1, 1.0~2.1, 율무 2.2~3.2, 1.0~2.1 log(CFU/g)검출되었으며, B. cereus은 찹쌀 1.0~2.1, 보리 2.1~3.1, 현미 2.4~2.6, 검정쌀 2.1~3.5, 들깨 1.0~2.1 log(CFU/g)로 검출되었다. Sal. spp은 검정깨에서 검출되었고, mold는 보리에서 검출되었다. 제조공정 중 미생물 오염도에 대해 조사한 결과 일반생균수는 입고단계의 경우 5.1~8.5 log(CFU/g)로 검출되었고, 세척 및 증자 공정은 4.4~7.5, 1.0~2.3 log(CFU/g)로 검출되었으며, 냉각, 건조, 방냉, 마쇄, 포장공정에서는 2.4~4.2, 1.0~4.0, 3.4~4.2, 4.3~5.2, 3.3~5.8로 검출되었다. 대장균군는 입고공정에서 1.0~2.01 (CFU/g)로 검출되었고, mold은 입고단계에서 검출되었다. 선식 가공업체의 환경 중 미생 물 결과 일반생균수는 원료탱크 40.2~7.5(CFU/g), 세척기와 건조기는 0.1~2.0, 0.1~3.2(CFU/g)로 검출되었고, 분쇄기, 포장지, 작업실은 3.2~4.0, 2.5~3.0, 3.8~5.2로 검출되었으며, 대장균군은 원료탱크와 분쇄기에서 각각 2.4~4.0, 0.0~4.1 log(CFU/g) 검출되었으며, 대장균은 검출되지 않았다. Mold은 원료탱크와 분쇄기, 작업실에서 검출되었다.