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Kinetic Behavior of Salmonella on Low NaNO2 Sausages during Aerobic and Vacuum Storage

  • Ha, Jimyeong;Gwak, Eunji;Oh, Mi-Hwa;Park, Beomyoung;Lee, Jeeyeon;Kim, Sejeong;Lee, Heeyoung;Lee, Soomin;Yoon, Yohan;Choi, Kyoung-Hee
    • Food Science of Animal Resources
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    • v.36 no.2
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    • pp.262-266
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    • 2016
  • This study evaluated the growth kinetics of Salmonella spp. in processed meat products formulated with low sodium nitrite (NaNO2). A 5-strain mixture of Salmonella spp. was inoculated on 25-g samples of sausages formulated with sodium chloride (NaCl) (1.0%, 1.25%, and 1.5%) and NaNO2 (0 and 10 ppm) followed by aerobic or vacuum storage at 10℃ and 15℃ for up to 816 h or 408 h, respectively. The bacterial cell counts were enumerated on xylose lysine deoxycholate agar, and the modified Gompertz model was fitted to the Salmonella cell counts to calculate the kinetic parameters as a function of NaCl concentration on the growth rate (GR; Log CFU/g/h) and lag phase duration (LPD; h). A linear equation was then fitted to the parameters to evaluate the effect of NaCl concentration on the kinetic parameters. The GR values of Salmonella on sausages were higher (p<0.05) with 10 ppm NaNO2 concentration than with 0 ppm NaNO2. The GR values of Salmonella decreased (p<0.05) as NaCl concentration increased, especially at 10℃. This result indicates that 10 ppm NaNO2 may increase Salmonella growth at low NaCl concentrations, and that NaCl plays an important role in inhibiting Salmonella growth in sausages with low NaNO2.

Rapid Nutrient Diagnosis of Cucumber by Test Strip and Chlorophyll Meter (Test Strip과 Chlorophyll Meter를 이용한 오이의 신속한 영양진단)

  • Kim, Kwon-Rae;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.5
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    • pp.272-279
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    • 2003
  • This study was performed to develop a more rapid and simple nutrient diagnosis method of plants than the conventional leaf analysis method. Cucumber (Cucumis sativus L. cv. jangil banbaek) was planted in the mixed media produced by mixing perlite and rock wool at 1:1 (v/v) ratio. The Yamazakki nutrient solution for cucumber was supplied to the media using micro-drip irrigation system. Experimental plots were consisted of no fertilization, deficient fertilization, adequate fertilization, and surplus fertilization for N, P and K. Specific color difference sensor value (SCDSV) measured by chlorophyll meter was closely related to total-N concentration in leaves measured by the conventional method. Nitrate, $PO_4$ and K concentration in petiole sap measured by test strips showed a significant relationship with total-N, P and K concentration in leaves. Linear regression equations between $NO_3$, $PO_4$ and K concentration in petiole sap and total-N, P and K concentration in the leaves were prepared. Optimum levels of $NO_{3}$, $PO_{4}$ and K in petiole sap were obtained by plugging the optimum concentrations of total-N, P and K in the leaves by other researchers into the equations. In conclusion, the SCDSV measured by chlorophyll meter and the concentration of $NO_3$, P and K in petiole sap measured by the test strips would be suitable for rapid estimation of plant nutrient status.

Relation of Acid Depositions and Chlorophyll Concentration in the Pinus thunbergii Leaf (산성강하물과 해송엽 엽록소의 관련성)

  • Lee, Chong-Kyu;Kim, Jong-Kab;Hwang, Jin-Hyeong
    • Journal of agriculture & life science
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    • v.45 no.2
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    • pp.35-41
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    • 2011
  • This study was carried out to investigate the relation of acid depositions and chlorophyll concentration in the Pinus thunbergii leaves. pH of throughfall in Pinus thunbergii forest was 4.20 at industrial sites, and 4.87 at urban sites. Total chlorophyll concentration in leaves were 0.5984 at industrial sites, and 0.6784 at urban sites. The average $SO_2$ concentration in air was 0.029ppm at industrial sites, and 0.011ppm at urban sites. Correlation between chlorophyll concentration in the Pinus thunbergii leaves and acid depositions was pH (r=-0.8769), ${NO_3}^-$ (r=0.6983), ${SO_4}^{2-}$ (r=0.8929), $SO_2$ (r=0.8738), and $NO_2$ (r=0.8523) (p<0.001). respectively. The estimation equation of chlorophyll concentration in the Pinus thunbergii leaf (Y) were given by Y=4.8979 + 0.7698pH + $0.8412{SO_4}^2$ + $0.8673SO_2$, + $0.8767NO_2$+ $0.8767{NO_3}^-$ ($r^2$=0.8873), which coefficients of determination was 0.88.

Serum homocysteine concentration in dogs with immunosuppressant-responsive enteropathy

  • Benvenuti, Elena;Pierini, Alessio;Gori, Eleonora;Bottero, Enrico;Pietra, Marco;Lippi, Ilaria;Meucci, Valentina;Marchetti, Veronica
    • Journal of Veterinary Science
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    • v.21 no.4
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    • pp.47.1-47.12
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    • 2020
  • Background: Homocysteine (HCY) was evaluated in healthy and chronic enteropathic dogs, however no studies on dogs with immunosuppressant-responsive enteropathy are available. Objectives: The aim was to evaluate serum HCY concentrations and its prognostic role in dogs with immunosuppressant-responsive enteropathy compared to healthy dogs. Methods: Serum HCY concentration was statistically compared between 24 healthy dogs and 29 dogs with immunosuppressant-responsive enteropathy. Correlation analyses between serum total protein, albumin (ALB), C-reactive protein (CRP), folate and cobalamin, and serum HCY concentration were performed in immunosuppressant-responsive enteropathic dogs. Results: The associations between serum HCY concentration and clinical, histological, endoscopic scores and follow-up were evaluated. Mean serum HCY concentration was higher in immunosuppressant-responsive enteropathic dogs compared to control dogs (30.22 ± 8.67 µmol/L vs. 5.26 ± 2.78 µmol/L; p < 0.0001). No association between serum HCY concentration and total protein, ALB, CRP, folate concentration as well as, clinical score, histological and endoscopic scores was found. A negative correlation between serum HCY concentration and cobalamin was noted (p = 0.0025, r = -0.54). No significant difference in HCY was found between responsive and non-responsive dogs or between survivors and non-survivors. Conclusions: Although, serum HCY concentration was higher in immunosuppressant-responsive enteropathy, its prognostic value remains unclear. However, further prospective, large-scale studies are warranted to better investigate the possible prognostic role of HCY in immunosuppressant-responsive enteropathic dogs.

Analysis of Air Quality Change of Cheonggyecheon Area by Restoration Project (청계천복원공사에 따른 청계천과 주변지역의 대기질 변화분석)

  • Jang, Young-Kee;Kim, Jeong;Kim, Ho-Jung;Kim, Woon-Soo
    • Journal of Environmental Impact Assessment
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    • v.19 no.1
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    • pp.99-106
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    • 2010
  • The project of Cheonggyecheon revived the 5.8 kilometer stream and it removed the cover of stream and Cheonggye elevated road. It was begin October of 2003 and completed October of 2005. The purpose of this study is to analyze the air pollution change of Cheonggyecheon area and neighboring area from before and after the project. The change of concentration is compared with an air monitoring station data and measurement data. The analyzed pollutants are $NO_2$, $PM_{10}$, heavy metal, VOC which are measured at Cheonggyecheon and neighboring area. As the results, $NO_2$ concentration shows 10 % decreases in Cheonggyecheon area and neighboring area shows 16 % decreases by Chenoggyecheon restoration, and $PM_{10}$ concentration shows 15 % decreases in Cheonggyecheon area and neighboring area shows 16 % increases. One of VOC, benzene is increased in Cheonggyecheon area compared with neighboring area but Toluene, Ethylbenzene, m+p Xylene increased in neighboring area. After the Cheonggyecheon restoration, The heavy metals are not shows the improvement, but $PM_{10}$ and $NO_2$ concentration improved more than the changes of neighboring area. These improvements of pollution due to reduction of transportation and clearing of elevated road by Cheonggyecheon restoration project.

A study on Sea-salt distribution of High-rise Apartments in Waterfront Area (워터프런트 지역 고층아파트의 해염분포에 관한 연구)

  • Doe, Geun-Young;Lee, Han-Seok;Song, Hwa-Cheol
    • KIEAE Journal
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    • v.6 no.1
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    • pp.33-38
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    • 2006
  • The purpose of this study is to investigate the concentration of sea-salt distribution giving great characteristic environmentally. Therefore the field measurement was conducted to target high-rise apartments constructing in reclaimed land of Busan and measured the concentration of sea-salt by means of distance and height of high-rise apartments from coast. The results of this study were as follows ; 1) The more a horizontal distance is far from coast, the more concentration sea-salt become low. But this study can not draw conclusion exactly as differ from a tendency of the thing close to the coast and the land. 2) In the near seaside, No.132 apartment accords existing research result that in difference of the concentration of sea-salt by height, the more height is high, the more that is high. But in seaside, No.117 apartment of middle point appears that the upper story and the lower story than middle story. Once more, in inland, No.132 apartment and No.117 apartment tend toward opposite it of the seaside. The reason that the result of this measurement and existing study is not agreement is thought that it occurs to turbulent flow between apartments by construction of high-rise apartments group.

Effect of NaCl on Nitrogen Content of Barley Seedlings

  • Kim, Choong-Soo;Cho, Jin-Woong;Lee, Sok-Young;Park, Kwan-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.1
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    • pp.4-10
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    • 1999
  • This study was conducted to determine the effects of NaCl stress on nitrogen, ${NH_4}^+$, and ${NO_3}^-$ content of 4 barley cultivar seedlings that were cultured for 10 and 30 days with different NaCl levels (0, 50, 100, and 150 mM) contain-ing 1/4 Hoagland solutions. The sodium ion content in the shoot of barley seedlings sharply increased with an increase of NaCl concentration. After 30 days of NaCl treatment, the sodium content of the shoot at 150 mM NaCl was 27 times higher than in non-saline conditions. The sodium content in the root linearly increased with increasing NaCl concentration. Nitrogen content in the shoot linearly increased with increasing NaCl concentration, but nitrogen content in the root declined above the point where the $Na^+$ content was 3.0 mM/g $Na^+$ in the barley seedling. ${NO_3}^-$ content also decreased with increasing NaCl concentration. ${NH_4}^+$ content in the shoot decreased with NaCl condition, but its content in the root increased with NaCl condition. A positive correlation between ${NO_3}^-$ and ${NH_4}^+$ content was found in the shoot, but their relationship was negative in the root.

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A Study on Denitrification Efficiency with External Carbon Sources in Pilot-scale Modified SBR (Pilot-scale modified SBR 공정에서 외부탄소원의 종류에 따른 탈질효율 비교 연구)

  • 서인석;김병군;홍성택;정위득
    • Journal of environmental and Sanitary engineering
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    • v.17 no.3
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    • pp.52-59
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    • 2002
  • Pilot-scale PSSBR(Phase Separated Sequencing Batch Reactor) was operated to evaluate requirement of external carbon sources(${\Delta}gCOD/{\Delta}gNO_3^{-}-N$) in denitrification. Methanol and fermented food waste were used as external carbon sources. Methanol and fermented food waste were fed to the anoxic state of first reactor and concentration were 50 and 40 mgCOD/L on the basis of concentration in reactor, respectively. In case that external carbon source was not used, average $NO_3^{-}-N$ concentration in effluent was 22.49 mg/L. When methanol and fermented food waste were fed, average $NO_3^{-}-N$ concentration in effluent were 10.13 mg/L and 6.3 mg/L, respectively and requirement of external carbon sources were 4.04 and 2.5 ${\Delta}gCOD/{\Delta}gNO_3^{-}-N$, respectively. Fermented food waste was better than methanol in denitrification efficiency. Therefore fermented food waste could be one of the excellent external carbon sources for nitrogen removal in biological nutrient removal process.

Effect of acupuncture hanggan and chungwan on serum lipid composition in rat fed high fat diet (행간(行間) 및 중완혈(中脘穴) 침자극(鍼刺戟)이 고지방급여(高脂肪給與) 흰쥐의 혈청지질구성(血淸脂質構成)에 미치는 영향(影響))

  • Lee Eun;Lee Joon-Moo
    • Korean Journal of Acupuncture
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    • v.20 no.1
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    • pp.31-38
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    • 2003
  • Effects of Haenggan($LR_2$) and Chungwan ($CV_{12}$) acupuncture on plasma lipid composition were investigated in rat fed high fat diet. Body weight gain showed a tendency to decrease in acupuncture groups and in acupuncture groups, chungwan acupuncture groups showed a lower values than haenggan acupuncture group. Concentrations of plasma triglyceride, free fatty acids showed a decrease in the acupuncture groups. Concentration of plasma lipoprotein showed a tendency to decrease in acupuncture groups, however in the control group and haenggan group, the values showed no significantly different. Plasma glucose concentration showed a low values than control group, in acupuncture groups, the values showed no significantly different. In plasma cholesterol concentration, total cholesterol and LDL-cholesterol showed a tendency to decrease in acupuncture groups, however the values of LDL-cholesterol showed no significantly different in the control group and haenggan group. HDL-cholesterol concentration showed no significantly different in all treatment groups.

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Effect of SO2 Concentration on NOx Removal Efficiency in NaOH-Based Wet Scrubbing (NaOH를 이용한 배기가스의 습식 스크러빙에서 SO2 농도에 따른 NOx 제거효율)

  • Kang, Myung Soo;Hwang, Jungho
    • Journal of Korean Society for Atmospheric Environment
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    • v.34 no.5
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    • pp.659-667
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    • 2018
  • $NO_x$ and $SO_2$ are mainly generated in the combustion of fossil fuels, and they cause secondary aerosol formation and acid rain in the atmosphere. Many studies have been conducted on the wet scrubbing process which can simultaneously reduce $NO_x$ and $SO_2$ at relatively low temperature. In this study, we conducted an experimental study on wet scrubbing by using NaOH solution. Especially, this study focuses on $NO_x$ and $SO_2$ removal characteristics by varying $NO_2/NO_x$ ratio and $SO_2$ concentration.