• Title/Summary/Keyword: Nitrophenol

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Construction of Bioluminescent Escherichia coli from lux Operon and Heat Shock Promoter for the Detection of Toxic Substances (lux Operon과 Heat Shock Promoter 유전자 재조합을 통한 독성물질 탐지용 대장균의 개발)

  • 유승오;이은관;김현숙;정계훈;전억한
    • Microbiology and Biotechnology Letters
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    • v.27 no.4
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    • pp.278-285
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    • 1999
  • In order to use heat shock promoter for the detection of toxic substances, dnaK promoter was amplified from E. coli genomic DNA by using a polymerase chain reaction(PCR) followed by sequencing and sub-cloning into the multi-cloning site of the plasmid, pUCD615. The pUCD615 is a broad-host-range vector containing promoterless lux operon originated from V.fischeri. The recombinant plasmid was transfered to E. coli DH5$\alpha$ through electroporation. The recombinant E. coli showed several patterns of bioluminescent responses to ethanol stress. The bioluminescent E. coli also showed responses to other toxic substances including FeK3(CN)6, CdCl2, p-nitrophenol and HgCl2. The increases of RLU(Relative Light Unit) were observed at 100ppm of FeK3(CN)6, 10ppm and 100ppm and 100ppm of CdCl2, 1ppm of 10ppm of p-nitrophenol and at 1ppm of HgCl2.

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Effect of scoparone on the hepatic sulfatransferase activity in mice

  • Huh, Keun;Park, Jong-Min;Shin, Uk-Seob;Lee, Sang-Il
    • Archives of Pharmacal Research
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    • v.13 no.1
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    • pp.51-54
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    • 1990
  • Effect of scoparone (6, 7-dimethoxycoumarin) on the hepatic cytosolic sulfotransferase activity was investigated. After treatment with scoparone, hepatic cytosolic sulfotransferase activity was increased with odse and time-dependent manner as compared to control. The $V_{max}$ value (control = 1.33 n moles/mg protein/min, scoparone = 2.39n moles/mg protein/min) without affecting the $K_m$ value for p-nitrophenol was increased by the scoparone treatment. Whereas, the hepatic cytosolic sulfotransferase was not changed by the addition of scoparone in vitro, and was strongly inhibited by the addition of metabolites of scoparone. The results obtained suggest that the characteristics of increase in the enzyme activity may include induction of enzyme proteins, and may be due to the metaboltes of scoparone.

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Development of Metal Complex Dyestuffs with High Fastness and High Adsorption (고견뢰도 및 고흡진율 특성의 금속 착염 염료의 개발)

  • Kim, Woon-Tai;Nam, Won-Woo;Sohn, Byoung-Chung
    • Journal of the Korean Applied Science and Technology
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    • v.12 no.2
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    • pp.115-120
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    • 1995
  • Diazotization of 2-Amino-5-nitrophenol was Synthesized by the direct diazotization method. The diazonium salts are usually stabilized in the from of double salts. The double salts of zinc chloride are the Commonest, and the double salt method is Specially applicable to the yield-up of coupling. The azo dye(2-naphthol-1-(2'-diazo-5'-nitrophenol)) is Converted into a chromium Complex by heating($100^{\circ}C$) with chromic formate, and DMF. The removal of inorganic salts from dyestuffs was performed in DMF solvent. This effect was more pronounced. From these results, It was obtained to liquid dyestuffs of metal complex with High fastness and high adsorption.

A Practical Synthesis of Morita-Baylis-Hillman Adducts of Aryl Vinyl Ketones Catalyzed by a Proton Donor

  • Kim, Sung-Hwan;Kim, Se-Hee;Lim, Cheol-Hee;Kim, Jae-Nyoung
    • Bulletin of the Korean Chemical Society
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    • v.33 no.6
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    • pp.2023-2027
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    • 2012
  • An efficient and practical synthesis of MBH adducts of aryl vinyl ketones was developed using DABCO and 4-nitrophenol as a proton donor. Addition of a proton donor and the use of excess amounts (3.0 equiv) of aldehydes were highly beneficial for the yields of MBH adducts of aryl vinyl ketones.

The Effect of Repeated Betaine Treatment on Hepatotoxicity and Cytochrome P-450 Dependent Drug Metabolizing Enzyme System (반복적인 Betaine 투여가 간독성 및 Cytochrome P-450 의존성 약물대사효소계 활성에 주는 영향)

  • Kim, Sang-Gyeom;Kim, Yeong-Cheol
    • YAKHAK HOEJI
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    • v.40 no.4
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    • pp.449-455
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    • 1996
  • Betaine is one of the major water-soluble components in Lycii Fructus. In the present study the effect of repeated betaine treatment on the hepatotoxicity and the cytochrome P-4 50-dependent enzyme system was examined in adult female rats. Administrations of betaine (100 or 1,000mg/kg/day, ip) to rats repeatedly for 4 or 9 days did not evoke hepatotoxic response as determined by increases in glutamic pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase(GOT) activities measured 24 hours following the final dose of betaine. The activities of aminopyrine N-demethylase, p-nitroanisole O-demethylase and p-nitrophenol hydroxylase as well as the contents of cytochrome P-450 were determined in hepatic microsomes of rats treated with betaine(1,000mg/kg/day, ip) for 4 or 9 days. Repeated treatment of rats with betaine for a period of 4 days induced a marginal decrease in the contents of cytochrome P-450, but did not influence the activities of p-nitrophenol hydroxylase, p-nitroanisole O-demethylase, or aminopyrine N-demethylase. Extension of the betaine treatment to 9 consecutive days failed to alter the parameters for hepatic drug metabolizing activity determined in the present study. Since repeated large doses of betaine were demonstrated to be tolerated by rats without showing any toxicity or changes in drug metabolizing enzyme activities in the liver, this compound appears to be relatively safe to animals upon long-term ingestion.

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A Study on the Treatment of Parathion Pesticide Using Marsh and Pond Type Constructed Wetlands (Marsh와 Pond 형태의 인공 습지를 이용한 Parathion 농약의 처리에 관한 연구)

  • Kim, Se-Kyung;Choi , Jong-Kyu;Oh, Se-Hee;Kang, Ho-Jeong;Zoh, Kyung-Duk
    • Journal of Environmental Health Sciences
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    • v.30 no.3
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    • pp.214-220
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    • 2004
  • The microcosm type wetland systems were constructed in order to treat wastewater contaminated with parathion. The microcosm reactor consisted of marsh and pond type. The experiment was carried out using batch (marsh or pond) and continuous (marsh-pond and pond-marsh type) systems. In the batch reactor, marsh-type wetland completely removed parathion in water within 8 days, while pond reactor removed 97% of parathion during the same period. During parathion degradation, the amount of 4-nitrophenol production, one of the metabolites from parathion degradation, was higher in marsh-type batch reactor. In the continuous systems, both marsh-pond and pond-marsh combination systems effectively removed parathion from water, and the production of 4-nitrophenol was also minimal. In the extraction experiment, the parathion and its metabolite were not found in the wetland soil and the plant. In order to achieve both aerobic and anaerobic conditions, the continuous wetland system combining marsh and pond type can be the alternative for the non-point source pollutants such as parathion pesticide.

Facile Synthesis of Gold Nanoparticles Using Tyrosine-Rich Peptide and Its Applications to Catalytic Reduction of 4-Nitrophenol (타이로신이 풍부한 펩타이드를 사용한 금 나노입자의 손쉬운 합성과 4-니트로페놀의 촉매 환원 응용)

  • Hur, Yun-Mi;Min, Kyoung-Ik
    • Applied Chemistry for Engineering
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    • v.32 no.1
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    • pp.15-19
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    • 2021
  • In this study, we studied a facile method for the synthesis of stable and nearly spherical gold nanoparticles using a tyrosine-rich peptide, Tyr-Tyr-Gly-Tyr-Tyr (YYGYY), as both the reducing and capping agent. The peptide coated spherical and polycrystalline gold nanoparticles with diameters from 3 to 15 nm were successfully synthesized by varying the concentration of the peptide and metal precursor under UV irradiation. The nanoparticles were then characterized by transmission electron microscopy (TEM), UV-Vis spectroscopy, scanning transmission electron microscopy-energy dispersive X-ray spectroscopy (STEM-EDS), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD). Furthermore, the catalytic activity of gold nanoparticles was confirmed by the reduction of 4-nitrophenol to 4-aminophenol, in which the catalytic reaction rate constant was 7.3 × 10-3 s-1.

甘味劑의 合成

  • Seong Jwa Gyeong
    • Journal of the Korean Chemical Society
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    • v.1 no.1
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    • pp.20-22
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    • 1949
  • 最近 2-Amino-1-nitrophenol 및 Alkyl ether의 甘味에 對한 News가 傳하여지고 있어 特히 n-Propylether는 의 4,000培의 甘味를 가졌다고한다. 本報에서는 그 合成條件을 檢사하였다. 同時에 2-Amino-4-nitro-toluene 2-Amino-4-nitro benzoic acid를 合成하여 그 甘味를 試驗하였다.

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