• Title/Summary/Keyword: Nitrogen transformation

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Inhibition of SKTI Synthesis in Agrobacterium rhizogenes-induced Hairy Root Reduces the Number of Nodule in Soybean (Kunitz Trypsin Inhibitor 발현 억제에 의한 콩 뿌리혹 수의 감소)

  • Kim, Sun-Hyung;Lim, Chae-Woo;Park, Ji-Young;Hwang, Cheol-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.3
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    • pp.299-306
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    • 2009
  • In nitrogen-limited conditions, rhizobia lead to formation of nitrogen-fixing nodules on the roots of leguminous plants. The process of nodulation is autoregulated by pre-existing nodules in the same root system. The altered profile of sap proteins by inoculation with B. japonicum may indicate presence of a signal responsible for autoregulation transferred through stem. The 20 kDa protein enhanced by innoculation significantly decreased in intensity from 2.5 to 7 days after inoculation (DAI). However 6 kDa protein did increase during such a transition period. Western blot analysis showed that both 20 kDa and 6 kDa were cross-reacted with the SKTI antiserum. This suggests that SKTI may be involved in soybean nodulation by specific induction and degradation in stem sap during early stage of nodulation. RNAi technique and Agrobacterium rhizogenes-mediated transformation were applied to investigate the function of SKTI in nodulation. We have found that the number of rhizobium-induced nodule was much less in SKTIi-silenced hairy roots than the non-silenced. Indeed the quantitative RT-PCR showed that the expression level of SKTI gene was reduced over 40% in the transgenic hairy roots compared to the non-transgenic. It appears that the observed early induction of SKTI and degradation into small peptide in a specific time manner may be involved in autoregulation of nodulation in soybean and the specific mechanism of such regulation remains to be investigated.

Influence of the nitrogen gas addition in the Ar shielding gas on the erosion-corrosion of tube-to-tube sheet welds of hyper duplex stainless steel (질소 보호 가스 첨가가 하이퍼 듀플렉스 스테인리스 밀봉용접재의 마모부식 저항성에 미치는 영향)

  • Kim, Hye-Jin;Jeon, Soon-Hyeok;Kim, Soon-Tae;Lee, In-Sung;Park, Yong-Soo
    • Corrosion Science and Technology
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    • v.13 no.2
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    • pp.70-80
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    • 2014
  • Duplex stainless steels with nearly equal fraction of the ferrite(${\alpha}$) phase and austenite(${\gamma}$) phase have been increasingly used for various applications such as power plants, desalination facilities due to their high resistance to corrosion, good weldability, and excellent mechanical properties. Hyper duplex stainless steel (HDSS) is defined as the future duplex stainless steel with a pitting resistance equivalent (PRE=wt.%Cr+3.3(wt.%Mo+0.5wt.%W)+30wt.%N) of above 50. However, when HDSS is welded with gas tungsten arc (GTA), incorporation of nitrogen in the Ar shielding gas are very important because the volume fraction of ${\alpha}$-phase and ${\gamma}$-phase is changed and harmful secondary phases can be formed in the welded zone. In other words, the balance of corrosion resistance between two phases and reduction of $Cr_2N$ are the key points of this study. The primary results of this study are as follows. The addition of $N_2$ to the Ar shielding gas provides phase balance under weld-cooling conditions and increases the transformation temperature of the ${\alpha}$-phase to ${\gamma}$-phase, increasing the fraction of ${\gamma}$-phase as well as decreasing the precipitation of $Cr_2N$. In the anodic polarization test, the addition of nitrogen gas in the Ar shielding gas improved values of the electrochemical parameters, compared to the Pure Ar. Also, in the erosion-corrosion test, the HDSS welded with shielding gas containing $N_2$ decreased the weight loss, compared to HDSS welded with the Ar pure gas. This result showed the resistance of erosion-corrosion was increased due to increasing the fraction of ${\gamma}$-phase and the stability of passive film according to the addition $N_2$ gas to the Ar shielding gas. As a result, the addition of nitrogen gas to the shielding gas improved the resistance of erosion-corrosion.

Nitrogen Transformation in Soil Cooperated with Organic Composts and Bio-char During Corn (Zea mays) Cultivation (옥수수 재배 시 퇴비 및 바이오차 시용 토양에서 질소 이동 동태)

  • Shin, JoungDu
    • Journal of the Korea Organic Resources Recycling Association
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    • v.22 no.3
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    • pp.33-40
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    • 2014
  • This study were conducted to evaluate the N mineralization and nitrification rates and to estimate the losses of total carbon and nitrogen by runoff water in soils cooperated with organic composts and bio-char during corn cultivation. For the experiment, the soil texture used in this study was clay loam, and application rates of chemical fertilizer and bio-char were $230-107-190kg\;ha^{-1}$($N-P_2O_5-K_2O$) as recommended amount after soil test and 0.2% to soil weight. The soil samples were periodically taken at every 15 day intervals during the experimental periods. The treatments were consisted of cow compost, pig compost, swine digestate from aerobic digestion system, and their bio-char cooperation. For N mineralization and nitrification rates, it was shown that there were generally low in the soil cooperated with bio-char as compared to the only application plots of different organic composts except for 47 days after sowing. Also, they were observed to be highest in the application plot of swine digestate from aerobic digestion system. For loss of total carbon by run-off water, it was ranged from 1.5 to $3.0kg\;ha^{-1}$ in the different organic compost treatment plots. However, Loss of total carbon with bio-char could be reduced at $0.4kg\;ha^{-1}$ in PC treatment plot. Also, with application of bio-char, total nitrogen was estimated to be reduced at 4.2 (15.1%) and $3.8(11.8%)kg\;ha^{-1}$ in application plots of pig compost and swine aerobic digestate, respectively.

Changes in Forms of Nitrogen and Phosphorus in Sediment by Growth of Zizania latifolia (줄(Zizania latifolia)의 생장에 의한 저토에서 질소와 인의 형태 변화)

  • Min, Keum-Sook;Kwak, Young-Se;Kim, Joon-Ho
    • The Korean Journal of Ecology
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    • v.28 no.2
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    • pp.93-98
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    • 2005
  • The effects of the presence of a submerged plant, Zizania latifolia, on physico-chemical characteristics, including Eh, pH, and concentrations of nitrogen and phosphorus in the sediments were studied under pot culture condition. It was shown that Eh value at reduced layer of the sediments was higher in the planted pots than in the non-planted. It was also revealed that $NH_4^+-N$ concentration of the sediments in the planted pots was lower than that of the non-planted, which might be due to the uptake by the plants. In contrast, $NO_3^--N$ concentration in the sediment increased in the presence of the plants compared to the non-planted, which might be attributed to oxygen released from the roots to the reduced layer. The concentration of organic phosphorus in the sediments was much higher than that of NAIP at the beginning of the planting experiment. However, at the end of the experiment, it was reversed; NAIP concentration was much higher than that of organic phosphorus, possibly indicating the transformation of organic phosphorus to NAIP during the experimental period. Both concentrations of $NH_4^+-N$ and $PO_4^{3-}-P$ in the overlying and percolated water were lower in the planted pots than in the non-planted. The concentration of $NO_3^--N$ in the percolated water, however, was higher in the planted pots than in the non-planted. The data was discussed with regard to the potential effects of a submerged plant on dynamics of phosphorus and nitrogen in the rhizosphere of the sediment.

Transformability and Phase Transformation Behaviors of Metastable Tetragonal Phase in 2Y-TZP Powder. (2Y-TZP 분말내 준안정 정방정상 입자의 전이도 및 상전이거동)

  • Kim, Hwan;Lee, Jong-Kook
    • Korean Journal of Materials Research
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    • v.2 no.5
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    • pp.320-329
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    • 1992
  • In 2Y-TZP powders calcined at temperature range of 80$0^{\circ}C$0 to 150$0^{\circ}C$, the effect of stabilization and the transformability of tetragonal phase on the tetragonal to monoclinic transformation have been investigated. The transformability of tetragonal phase in calcined powders shows maximum at the calcination temperature of 130$0^{\circ}C$. This result is explained by a combined effect of the increase of particle size and of constrained force among the particles with increasing the calcination temperature. The amount of transformed monoclinic phase with calcination temperature after quenching in liquid nitrogen, stress induction and isothermal aging at 25$0^{\circ}C$is also explained by the transformability of tetragonal phase determined by the sum of particle size effect and constraint effect.

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Synthesis of amorphous Fe-Cr-N alloy powders by mechanical alloying (기계적 합금화에 의한 Fe-Cr-N 계 비정질 합금분말의 제조)

  • 이충효
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.1
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    • pp.132-136
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    • 1999
  • Mechanical alloying (MA) by ball mill of $Fe_{30}Cr_{70}$ elemental powders was carried out under the nitrogen gas atmosphere. Amorphization has been observed in this case, while MA under an inert argon gas atmosphere produces the bcc solid solution. The DSC spectrum for the mechanically alloyed $(Fe_{30}Cr_{70})_{0.85}N_{0.15)$powders exhibits a sharp exothermic peak due to crystallization at about $550^{\circ}C$. Structural transformation from the bcc crystalline to amorphous states was observed through X-ray and neutron diffractions. During amorphization process the octahedral unit, which is typical of a polyhedron formed in any crystal structures, was preferentially destroyed and transformed into the tetrahedral unit. Futhermore, neutron diffraction measurements revealed that a nitrogen atom is situated at a center of the tetrahedron formed by metal atoms.

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Bioconversion of flowers waste: Composting using dry leaves as bulking agent

  • Sharma, Dayanand;Yadav, Kunwar D.
    • Environmental Engineering Research
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    • v.22 no.3
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    • pp.237-244
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    • 2017
  • At present, in India, handling of solid waste has become a major challenge for the municipal authorities. Composting of solid waste, especially organic waste, can be one of the solutions to tackle the issue of handling solid waste. The present study is focused on agitated piles composting of flower waste (FW). Five combinations of FW with dry leaves (DL) and cow dung (CD) were prepared to conduct the study. Significant changes were observed due to the addition of bulking agent. The bulking material helps to reduce the production of leachate and also to maintain the aerobic condition within the piles. The reduction of total organic carbon was 21% in FW composting which increased by 36.48% during the composting of FW on addition of DL and CD. On the 120th day of composting, the pH of pile five (70 kg FW + 20 kg CD + 15 kg DL) was 7.33, electrical conductivity 2.77 mS/cm, total organic carbon 26.9%, total nitrogen 2.2%, and C:N ratio was 12. Appropriate proportion of waste mixture played an important role in providing favorable conditions for the microbial transformation of flower waste to stabilized compost. Finally, FW with the combination of CD and DL was found to be successful during pile composting.

Effective Nitridation of Compacts of Coarse Silicon Particles (조립자규소 성형체의 효과적 질화가열법에 관한 연구(Densification of Silicon Nitride 3보))

  • 박금철;최상욱
    • Journal of the Korean Ceramic Society
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    • v.21 no.1
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    • pp.33-40
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    • 1984
  • To find out the optimum heating profile for the nitridation of compacts of graded silicon grains (max 53$mu extrm{m}$) two batches with the addition of MgO and $Mg(NO_3)_3$$cdot$$6H_2O$ to silicon particles were isostatically pressed into compacts. They were nitrided under some different nitriding schedules. The properties such as bulk densitis microstructures and formed phases were measured and observed. The following results were obtained ; 1) About 10% unreacted silicon remained in specimen which was nitrided at 1, 350$^{\circ}C$ for 240hrs. 2) One of the step-heating processes 1, 150$^{\circ}C$-1, 390$^{\circ}C$ for 65hrs are then $1, 390^{\circ}C$for 50hrs was the low temperature but with that at high temperature. 3) High pressure(10.5kgf/$cm^2$) of nitrogen at 1, 390$^{\circ}C$ accelerated the $\alpha$$ ightarrow$$\beta$ transformation of silicon nitride. 4) Magnesium nitrate was superior to magnesium oxide in the role of nitriding aid and the formation of uniform microstructures.

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Synthesis and Characterization of Sol-Gel Derived Mesoporous Titania/Alumina Membranes (솔젤법에 의한 메조기공 티타니아/알루미나 막의 제조 및 기체투과 특성)

  • Kwon, Hyuk-Taek;Kim, Jin-Soo
    • Membrane Journal
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    • v.21 no.3
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    • pp.229-235
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    • 2011
  • In this study, mesoporous titania/alumina membranes were prepared by sol-gel method. Pore structure and phase composition of titania/alumina membranes could be changed by calcination temperature. The addition of alumina into titania membranes retarded anatase-to-rutile phase transformation, resulting in stabilization of pore structures. The 5 time dip-coated membrane calcined at $450^{\circ}C$ is about $10.3{\mu}m$ in thickness with an average pore size of 5 nm. Hydrogen and nitrogen permeances through the membrane were $17.1{\times}10^{-7}mol/m^2{\cdot}s{\cdot}Pa$ and $4.7{\times}10^{-7}mol/m^2{\cdot}s{\cdot}Pa$, respectively. These data were explained by the Knudsen diffusion mechanism.

Transformations of 2,4,6-Trinitrotoluene in Various Conditions by Klebsiella sp. Strain C1 Isolated from Activated Sludge

  • Chang, Chong-Suk;Kim, Hyoun-Young;Kang, Yang-Mi;Bae, Kyung-Sook;Song, Hong-Gyu
    • Journal of Microbiology
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    • v.40 no.3
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    • pp.193-198
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    • 2002
  • Several 2,4,6-trinitrotoluene (TNT) degrading bacteria were isolated from an activated sludge by an enrichment culture technique, and their TNT removal activities were examined. Among the isolates, strain C1 showed the highest degrading capability, and completely removed 100 or 200 mg I$\^$-1/ of TNT within 6 hours of incubation. This bacterium was identified as Klebsiella sp. The effects of different carbon sources on the removal of the parent TNT by Klebsiella sp. C1 were negligible, but the transformation rates of TNT metabolites such as amino-dinitrotoluenes and diamino-nitrotoluenes were higher with fructose addition compared to glucose addition. When nitrate was used as the nitrogen source, the degradation rates of TNT and hydroxylamino-dinitrotoluenes were higher than those with the ammonium addition. Although the TNT removal rate of Klebsiella sp. C1 was slightly higher in anaerobic conditions, the further transformations of TNT metabolites were more favorable in aerobic conditions.