• Journal of Microbiology and Biotechnology
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• v.27 no.3
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• pp.561-572
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• 2017

The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.7
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• pp.485-491
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• 2011

This study was performed to investigate the characteristics of nutrient removal of municipal wastewater in membrane bioreactor system. Membrane bioreactor consists of four reactors such as two intermittently anaerobic tanks, the oxic tank and the sludge solubilizaion tank with an internal recycle. The hydraulic retention time (HRT) and flux were 6.5 hours and $20.4L/m^2{\cdot}hr$ (LMH), respectively. The removal efficiency of $COD_{Cr}$, SS, TN and TP were 94.0%, 99.3%, 99.9%, 69.9%, and 66.9%, respectively. The estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR), specific phosphorus release rate (SPRR) and specific phosphorus uptake rate (SPUR) were 0.34 kgVSS/kgBOD d, $0.067mgNO_3-N/mgVSS{\cdot}d$, $0.028mgNH_4-N/mgVSS{\cdot}d$, 16.0 mgP/gVSS d and 2.1 mgP/gVSS d, respectively. The contents of nitrogen and phosphorus of biomass were 8.9% and 3.5% on an average.

• Proceedings of KOSOMES biannual meeting
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• 2006.11a
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• pp.97-104
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• 2006

Though stringent guidelines are in place to protect the harbor environment, pollution from ships, from the ports terminals. Discharge from the ballast tanks of ships, though illegal, does occur. Such vessels, arriving from distant ports of call, can introduce exotic species of plants and animals, causing disruption of the local food web. Discharges rich in nitrogen can generate the rapid growth of plankton, eventually leading to a condition known as red tide that is lethal to some coastal organisms. In addition to the harbor's negative effects on marine organisms, the diesel engines of the ships and the trucks that haul cargo to and from the ports release large volumes of diesel exhaust into the atmosphere. IMO(International Maritime Organization) is strongly proceeding with adoption of a new maritime environment convention and coming into effect for regulation enhancement about the pollutants which are happened in a ship recently. Study about the conventions that our country currently comes into effect, and there is during forwarding and correspondence must be performed effectively. In this paper, International convention on the control of harmful Anti-Fouling system on ship, Ballast water management, Prevention of air pollution from ships, treat a main pending problem in ocean related environmental regulation convention.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.3
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• pp.358-365
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• 1999

The effects of dry roasting whole lupin seeds (lupinus albus, WLS) at 110, 130 or $150{^{\circ}C}$ for 15, 30 or 45 minutes on the in sacco rumen degradation characteristics, optimal heating conditions of time and temperature and in vitro enzyme digestibility were determined. Ruminant degradation characteristics (RDC) of crude protein (CP) of WLS were determined by in sacco technique in dairy cows. Measure ROC were soluble (S), undegradable (U), potentially degradable (D) fractions, lag time (TO) and rate of degradation (Kd) of insoluble but degradable fraction. Based on measured ROC, percentage bypass CP (%BCP) and bypass CP (BCP in g/kg, DM) were calculated. Degradability of CP was significantly reduced by dry roasting (p<0.001). The interaction of dry roasting temperature and time had significant effects on D (p<0.05), Kd (p<0.01), U (p<0.01), %BCP (p<0.001) and BCP (p<0.001) but not on S (p=0.923>0.05). With increasing time and temperature, S, D, Kd and U varied from 31.8%, 67.4%, 10.3%/h and 0.8% in the raw WLS (RWLS) to 27.1 %, 35.8%, 3.6%/h, 38.4% in $150{^{\circ}C}/45\;min$, respectively. All these effects resulted in increasing %BCP from 25.9 in RWLS to 61.0% in the $150{^{\circ}C}/45\;min$. Therefore BCP increased form 111.2 to 261.2 g/kg DM, respectively. Both %BCP and BCP at $150{^{\circ}C}/45\;min$ increased nearly 2.5 times over the RWLS. The effects of dry roasting on %BCP and BCP seemed to be linear up to the highest value tested. Although ROC had been altered by dry roasting, the In vitro perpsin-cellulase digestibility was generally unchanged. It was concluded that dry roasting was effective in shifting CP degradation from rumen to the lower gastrointestinal tract to potential reduce unnecessary N loss in the rumen. It might be of great value in successfully synchronizing the rhythms of release of nitrogen and energy in the rumen, thus achieving a more efficient fermentation of diets with high proportions of lignocellulosic resources. To determine the optimal dry roasting conditions, the digestibility of each treatment in the cows will be measured in the next trial using mobile bags technique.

• Journal of the Korean Vacuum Society
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• v.9 no.2
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• pp.155-161
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• 2000

BN films composed of c-BN(70%) and h-BN(30%) phases have been synthesized by the ion beam assisted deposition (IBAD) process and stabilized by post-annealing. Boron was e-beam evaporated at 1.2 $\AA$/sec and nitrogen was ionized and accelerated at about 100 eV by the end-hall type ion gun. Substrates were negatively biased by DC 400 and 500 V, respectively, and heated at $700^{\circ}C$. Synthesized BN films were in-situ post-annealed at 700 or $800^{\circ}C$, respectively, for 1 hr without breaking vacuum. BN films without post-annealing were peeled off from substrates immediately when they were exposed to the air while those with post-annealing at $800^{\circ}C$ were stabilized. Post annealing reduced the film stress from 4.9 GPa to 3.4 GPa, but no considerable stress release in the c-BN phase was observed, contrary to previous reports that the stress relaxation in the c-BN phase is the main mechanism for the stabilization. Structural and chemical relaxation of non c-BN phase is supposed to be responsible for the film stress reduction and, in turn, stabilization, especially when the c-Bn content of the film is not high.

• Journal of Wetlands Research
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• v.18 no.1
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• pp.68-75
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• 2016

A set of lab-scale polymer synthetic fiber packed column wetlands composing three columns (CW1, CW2 and CW3) with different hydraulic regimes, recirculation frequencies and pollutant loading rates, were operated in 2012. Synthetic fiber tested as an alternative wetland medium for soil mixture or gravel which has been widely used, has very high pore size and volume, so that clogging opportunity can be greatly avoided. The inflow to the wetland was artificial stormwater. All the wetlands achieved effective removal of TSS (94%~96%), TCOD (68%~73%), TN (35%~58%), TKN (62%~73%) and NH4-N (85%~ 99%). Particularly, it was observed that COD was released from the fiber during one distinct period in all wetlands. This was probably due to the degradation of polymer fiber, and the released organic matters were found to serve as carbon source for denitrification. In addition, with longer retention time and frequent recirculation, lower effluent concentration was observed. With higher pollutant loading rate, higher nitrification and denitrification rates were achieved. However, although organic matters were released from the fiber, the lack of carbon source was still the limiting factor for the system since the release persisted only for 40 days.

• Biomedical Science Letters
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• v.12 no.3
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• pp.289-301
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• 2006

Cardiopulmonary bypass (CPB) for cardiac surgery triggers the production and release of numerous chemotactic substances and cytokines, ensuing systemic inflammatory response that leads to postoperative major organ dysfunction. Traditionally, corticosteroids (steroid) have been administered to patients undergoing cardiac surgery to ward off these detrimental physiologic alterations. However, the majority of the studies have been performed on adult patients with high-dose steroid. We carried out a randomized, prospective, double-blind study to compare the efficacy of low-dose steroid with that of high-dose steroid and to determine the adequate dose of pretreated-steroid for prophylactic effects in pediatric cardiac surgery. Thirty pediatric patients scheduled for elective cardiac surgery were randomly assigned to two groups; fifteen patients received low-dose methylprednisolone (10mg/kg intravenously, n=15, low-dose group) and the others received high-dose methylprednisolone (30mg/kg intravenously, n=15, high-dose group) 1 hour prior to CPB. Arterial blood samples were taken before CPB (Pre-CPB), 10 minutes after start of CPB (CPB-10), and immediately after CPB-end (CPB-OFF) for measuring total leukocyte counts (T-WBC) and diff-counts, platelet counts, interleukin-6 (IL-6), myeloperoxidase (MPO), total antioxidant (TAO), neuron-specific enolase (NSE), troponin I (TNI), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, and blood urea nitrogen (BUN) levels. Other parameters such as volumes of urine output, pulmonary index $(PI,\;PaO_2/FiO_2)$, mechanical ventilating period, intensive care unit (ICU)-staying period, postoperative complications (fever, wound problem), postoperative 24 hrs and total volumes in blood loss, and hospitalized days were also assessed. All parameters were compared between two groups. There were no significant differences in T-WBC counts, monocyte fraction, platelet counts, TA levels, NSE levels, creatinine levels, BUN levels, the volumes of total urine output, PI, the incidences of fever and wound problem, postoperative 24hrs- and total-blood loss volumes and ICU-staying period between two groups (P>0.05). At CPB-OFF, neutrophil fraction, MPO level, TNI level, and AST level were higher in the high-dose group than in the low-dose group (P<0.05). IL-6 level at CPB-10 was higher in the high dose-group than in the low-dose group (P<0.05). Furthermore, mechanical ventilating periods and hospitalized days of the high-dose group were significantly longer than those of low-dose group (P<0.05). The high-dose group had significantly low lymphocyte fi-action at CPB-OFF compared with the low-dose group (P<0.001). These findings suggest that pretreatment of high-dose steroid is not superior to that of low-dose steroid regrading its potential benefits in pediatric cardiac surgery. Therefore, the conventional strategy of steroid treatment, high-dose pretreatment, should be modified in the cardiac surgery with CPB. However, further studies must be performed on the larger number of patients in as much as small number of patients in this study.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.4
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• pp.159-168
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• 2015

Forty-four surface sediments from Doam Bay were analyzed for total organic carbon (TOC), total nitrogen (TN), total metal (Al, Fe, Mn, Cr, Cu, Ni, Pb, Zn) and further chemical partitioning of metals were carried out in some samples. The TOC (0.32~3.10%) and TN (0.03~0.26%) values of the samples were similar to those of other coastal area. The C/N ratios ranged from 7.9 to 11.9 with an average 9.3 which revealed that contribution of terrestrial organic matters was relatively rare. Contents of analysed metals showed a level lower than threshold effects level (TEL) in sediment quality guidelines. Based on the chemical speciation of metals, the lattice fractions were found in the order Cr > Cu > Ni > Zn > Pb > Mn, while Mn and Pb are the ratio of the non-lattice fractions accounted for more than 50%. The average baseline values were obtained relative cumulative frequency curves and linear regression analysis. The respective baseline concentrations for Cu, Ni, Pb, Zn, Cr and Mn were 11.8, 23.1, 26.8, 76.6, 56.7, 585 mg/kg, respectively. Based on geoaccumulation index ($I_{geo}$) with a baseline values of Mn showed that face the contamination phase from estuarine stations. However, in case of Zn and Pb, although there is no sign of contamination, it could be release from sediment when there is a change in the environment, which is caused from the high ratio of non-lattice fractions.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.2
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• pp.100-104
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• 1989

This study was carried out in order to develop a compound fertilizer for raising rice seedling in trays. A compound fertilizer, a trail product, was manufactured using the major fertilizer sources of ammonium sulfate, diammonium phosphate and potassium chloride in combination with zeolite and glutamic acid fermentation waste. Besides, polyacrylamide for slow release control of the fertilzer and Tachigaren and sulfuric acid for reducing the occurrence of seedling rot were used. The component ratios of N, $P_2O_5$ and $K_2O$ of trial product were 4.19, 5.41 and 4.05 percent respectively. The dissolution rate of nitrogen component in water for the trial product with polyacrylamide was lower about fifteen percents than the product without polyacylamide in six hours. Hymexazole, main component of Thachigaren, from the product was released about 86.2 percents in forty eight hours. When the product with polyacylamide applied on red earth soil and paddy soil, the pH of soil ranged from 4.6 to 5.4 for 25 days experiment.

• Food Industry And Nutrition
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• v.9 no.1
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• pp.36-45
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• 2004

Bamboo salt has been used for the purpose of prevention and treatment of various diseases in Korea. Present study was carried out to ascertain the effects of purple bamboo salt upon anti-allergic effect, anti-inflammatory activity and immune-enhance effect as well. Purple bamboo salt significantly inhibited the ear swelling response and histamine release induced by compound 48/80 in mice and rat peritoneal mast cells. Purple bamboo salt (0.01 ∼ lg/kg) also dose-dependently inhibited the passive cutaneous anaphylaxis by oral administration. Purple bamboo salt (1 mg/mL) in hibited phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-1${\beta}$ and IL-6 secretion, by 67.04${\pm}$0.08%, 68.01${\pm}$1.85%, 69.48${\pm}$0.54%, respectively. In addition, purple bamboo salt inhibited the expression of TNF-${\alpha}$ mRNA in HMC-1 cells. Finally, we investigated the effect of purple bamboo salt in the forced swimming test (FST) and the change of purple bamboo salt-mediated cytokine production from MOLT-4 cells. At the 7th, immobility time was significantly decreased in the purple bamboo salt-administration group (35.4 ${\pm}$5.9 s for 1 g/kg) in comparison with the control group (93.2 ${\pm}$ 15.45). After FST, the content of glucose in the blood serum was increased and the levels of blood urea nitrogen, lactic dehydrogenase was decreased in purple bamboo salt-administration group. However, it had no effect on the elevation of CK and TP level. Purple bamboo salt (1 mg/mL) significantly increased the interferon (IFN)-${\gamma}$ and IL-2 level compared with media control (about 3.7-fold for IFN-${\gamma}$, about 3.5-fold for IL-2, p〈0.05) but did not affect the IL-4.