• Title/Summary/Keyword: Nitrogen rate

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Variations in carbon and nitrogen stable isotopes and in heavy metal contents of mariculture kelp Undaria pinnatifida in Gijang, southeastern Korea

  • Shim, JeongHee;Kim, Jeong Bae;Hwang, Dong-Woon;Choi, Hee-Gu;Lee, Yoon
    • ALGAE
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    • v.32 no.4
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    • pp.349-357
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    • 2017
  • Korean mariculture Undaria pinnatifida was collected during the months of January, February, March, and December of 2010, as well as from January of 2011 to investigate the changes in the carbon and nitrogen stable isotope ratios (${\delta}^{13}C$ and ${\delta}^{15}N$) and heavy metal with respect to it growth and to identify the factors that influence such changes. The blades of U. pinnatifida showed ${\delta}^{13}C$ and ${\delta}^{15}N$ in the range (mean) of -13.11 to -19.42‰ (-16.93‰) and 2.99 to 7.57‰ (4.71‰), respectively. Among samples with the same grow-out period, those that weighed more tended to have higher ${\delta}^{13}C$ suggesting a close association between the carbon isotope ratio and growth rate of U. pinnatifida. Indeed, we found a very high positive linear correlation between the monthly average ${\delta}^{13}C$ and the absolute growth rate in weight ($r^2=0.89$). Nitrogen isotope ratio tended to be relatively lower when nitrogen content in the blade was higher, probably due to the strengthening of isotope fractionation stemming from plenty of nitrogen in the surrounding environment. In fact, a negative linear correlation was observed with the nitrate concentration in the nearby seawaters ($r^2=0.83$). Concentrations of Cu, Cd, Pb, Cr, Hg, and Fe in the blades showed a rapid decrease in their concentration per unit weight in the more mature U. pinnatifida. Specifically, compared to adult samples, Cu, Hg, and Pb were concentrated by 30, 55, and 73 folds, respectively, in the young blades. Therefore, U. pinnatifida tissue ${\delta}^{13}C$ is as an indirect indicator of its growth rate, while ${\delta}^{15}N$ values and heavy metal concentrations serve as tracers that reflect the environmental characteristics.

Effect of Nitrogen Top-dressing Method on Grain Quality of Barley in Japanese Volcanic Ash Soil (일본 화산회토에서 질소 시용방법이 보리의 품질에 미치는 영향)

  • 이춘우
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.4
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    • pp.512-517
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    • 1995
  • Effects of nitrogen top-dressing method on quality of barley were evaluted over amount of 2 and 4 kg per 10a on volcanic ash soil in Japan under regrowth stage, productive tiller stage and heading stage. 55% milling time was longer by the amount of top- dressing, but that was not influenced by the times of top-dressing. And glassiness, whiteness, hardness and 8 minute- millng rate were not influenced by times and amounts of top-dressing. Crude protein rate of grain was increased by amount of nitrogen top-dressing, but that did not correlated with time of nitrogen top-dressing. Crude protein rate of grain was positively correlated with 55% milling time. But that did not correlated with whiteness of 8 minute-milled grain. 8 minute-milling rate was negatively correlated with whiteness of 8 minute-milled grain. There was no visible difference of protein matrix with scanning electron microscope in different nitrogen levels.

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Removal of Low Concentration Ammonia Nitrogen using a Packed Bed Bioreactor Immobilized with Nitrifier Consortium (질화세균을 고정화한 충전층 생물반응기에서 저농도 암모니아성 질소 제거)

  • Lee, Chang-Keun;Kim, Byong-Jin;Lee, Min-Su;Kim, Yong-Ha;Suh, Kuen-Hack
    • Clean Technology
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    • v.13 no.1 s.36
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    • pp.16-21
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    • 2007
  • This study estimated the effect of hydraulic residence time(HRT), influent total ammonia nitrogen(TAN) concentration, temperature and pH in the packed-bed bioreactor using immobilized nitrifiers. Removal rate of ammonia nitrogen was increased with decreasing HRT and the optimum HRT was 0.2 hour when influent TAN was $2g/m^3$. At this point, removal rate was $226.1\;g/m^3{\cdot}day$ and removal efficiency was 88.8%. Removal rate of ammonia nitrogen was Increased with increasing TAN concentration. Removal rate and efficiency of ammonia nitrogen were kept constant at $20{\sim}35^{\circ}C$ and pH $8{\sim}9$ value.

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Effect of Nitrogen on Cell Dynamics at Leaf Growth Zone in Two Rice Varieties

  • Sung, Jwa-Kyung;Lee, Chul-Won;Kim, Tae-Wan;Hwang, Seon-Woong;Song, Beom-Heon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.2
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    • pp.121-125
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    • 2004
  • In plants, nitrogen is the major component for growth and development. Leaf growth is based on the division, elongation and maturation of cells, which are used for making of epidermis, mesophyll, bundle sheath, xylem, phloem and so on. Dynamics of these tissues with respect to nitrogen are required for better understanding. This experiment was conducted to evaluate effect of nitrogen on the elongation of epidermal and guard cell of two rice (Oryza sativa L.) varieties, Seoanbyeo and Dasanbyeo on May 2000 at Chungbuk national university in Cheongju. After transplaning the 20-day-old seedlings into a/5000 pots, the main characteristics related with cell elongation were investigated and evaluated. A maximum. leaf length reached at 7 or 8 days after emerging from the collar, and also the leaf elongation rates were greatly affected by the increase of N application rate. The initial and final cell length were about $17\mu\textrm{m}$ and $130\mu\textrm{m}$, respectively. Cell divisions occurred within 1.0mm from leaf base. With die higher nitrogen application rate of 22 kg-N $10\textrm{a}^{-1}$, cell division per hour was greater 1.5 to 1.9 and 1.2 to 1.3 fold as compared to the N application rate of 0 and 11 kg-N $10\textrm{a}^{-1}$, respectively. Cell enlargement of epidermal and guard cell under higher N application rate (22kg-N $10\textrm{a}^{-1}$) was finished within about 20 (Seoanbyeo) and 15 hours (Dasanbyeo), while it took much time, about 30 hours.

Nutrient dynamics in montane wetlands, emphasizing the relationship between cellulose decomposition and water chemistry

  • Kim, Jae Geun
    • Journal of Wetlands Research
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    • v.7 no.4
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    • pp.33-42
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    • 2005
  • Wetlands often function as a nutrient sink. It is well known that increased input of nutrient increases the primary productivity but it is not well understood what is the fate of produced biomass in wetland ecosystem. Water and sediment quality, decomposition rate of cellulose, and sediment accumulation rate in 11 montane marshes in northern Sierra Nevada, California were analyzed to trace the effect of nitrogen and phosphorus content in water on nutrient dynamics. Concentrations of ammonium, nitrate, soluble reactive phosphorus (SRP) in water were in the range of 27 to 607, 8 to 73, and 6 to 109 ppb, respectively. Concentrations of ammonium, calcium, magnesium, sodium, and potassium in water were the highest in Markleeville, which has been impacted by animal farming. Nitrate and SRP concentrations in water were the highest in Snow Creek, which has been impacted by human residence and a golf course. Cellulose decomposition rates ranged from 4 to 75 % per 90 days and the highest values were measured in Snow Creek. Concentrations of total carbon, nitrogen, and phosphorus in sediment ranged from 8.0 to 42.8, 0.5 to 3.0, and 0.076 to 0.162 %, respectively. Accumulation rates of carbon, nitrogen, and phosphorus fluctuated between 32.7 to 97.1, 2.4 to 9.0, and 0.08 to $1.14gm^{-2}yr{-1}$, respectively. Accumulation rates of carbon and nitrogen were highest in Markleeville and that of phosphorus was highest in Lake Van Norden. Correlation analysis showed that decay rate is correlated with ammonium, nitrate, and SRP in water. There was no correlation between element content in sediment and water quality. Nitrogen accumulation rate was correlated with ammonium in water. These results showed that element accumulation rates in montane wetland ecosystems are determined by decomposition rate rather than nutrient input. This study stresses a need for eco-physiological researches on the response of microbial community to increased nutrient input and environmental change because the microbial community is responsible for the decomposition process.

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Effect of $N_2$ and $O_2$ Properties of STS304 Stainless Steel Films Synthesized by Unbalanced Magnetron Sputtering Process (비대칭 마그네트론 스퍼터링법에 의해 합성된 STR304 스테인리스강 박막에서의 질소와 산소의 첨가 효가)

  • 김광석;이상율;김범석;한전건
    • Journal of the Korean institute of surface engineering
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    • v.34 no.2
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    • pp.89-96
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    • 2001
  • N- or O-doped STS304 stainless films were synthesized by an unbalanced magnetron sputtering process with various argon and reactive gas ($N_2$, $O_2$) mixtures. These films were examined by scanning electron microscopy (SEM), X-ray diffraction (XRD), Auger electron spectroscopy (AES) and Knoop microhardness tester. The Results from X-ray diffraction (XRD) analysis showed that a STS304 stainless steel film synthesized without reactive gas using a bulk STS304 stainless steel target had a ferrite bcc structure ($\alpha$ phase), while the N-doped STS304 stainless film was consisted of a nitrogen supersaturated fcc structure, which hsa a strong ${\gamma}$(200) phase. In the O-doped films, oxide Phases ($Fe_2$$O_3$ and $Cr_2$$O_3$) were observed from the films synthesized under an excess $O_2$ flow rate of 9sccm. AES analysis showed that nitrogen content in N-doped films increased as the nitrogen flow rate increased. Approximately 43 at.%N in the N-doped film was measured using a nitrogen flow rate of 8sccm. In O-doped film, approximately 15 at.%O was detected using a $O_2$ flow rate of 12sccm. the Knoop microhardness value of N-doped film using a nitrogen flow rate of 8 sccm was measured to be approximately $H_{ k}$ 1200 and this high value could be attributed to the fine grain size and increased residual stress in the N-doped film.

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Effects of Mixed Organic Fertilizer Application with Rice Cultivation on Yield and Nitrogen Use Efficiency in Paddy Field (벼 재배시 혼합유기질비료 시용이 질소이용율과 수량에 미치는 영향)

  • Cho, Kwang-Rae;Won, Tae-Jin;Kang, Chang-Sung;Lim, Jae-Wook;Park, Kyeong-Yeol
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.3
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    • pp.152-159
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    • 2009
  • This study was carried out to investigate optimum application rate with mixed organic fertilizer for chemical fertilizer alternative where the paddy rice (Chucheong) was cultivated in clay loam of paddy field. The mixed organic fertilizer were applied at 0, 50, 100 and 150% levels of recommended nitrogen by soil testing plot compared with plot of chemical fertilizer (nitrogen, phosphate and potash), respectively. The ammonium nitrogen content in paddy soil and surface water of mixed organic fertilizer 100% plot were higher than chemical fertilizer plot. The absorbed amount of nitrogen and nitrogen use efficiency by rice plant in mixed organic fertilizer 100% plot were higher than chemical fertilizer plot. The number of tillers on rice at 30 days and 60 days after transplanting mixed organic fertilizer 100% plot were many more than chemical fertilizer plot. The milled rice yield of mixed organic fertilizer 100% plot was increased by 4% than chemical fertilizer plot. The optimum application rate of mixed organic fertilizer was as follows ; $OAR_{MOF}(Mg\;ha^{-1})=[(NAR_{ST}{\div}NC_{MOF}/1,000{\times}0.93)/1,000]$ ($OAR_{MOF}$ : Optimum application rate of mixed organic fertilizer, NARST : Nitrogen application rate($kg\;ha^{-1}$) by soil testing, $NC_{MOF}$ : Nitrogen content($g\;kg^{-1}$) of mixed organic fertilizer, and $0.93:124.3kg\;ha^{-1}/133.0kg\;ha^{-1}$, respectively).

Use of Hairy Vetch Green Manure as Nitrogen Fertilizer for Corn Production

  • Seo, Jong-ho;Lee, Ho-jin;Hur, Il-bong;Kim, Si-ju;Kim, Chung-kuk;Jo, Hyeon-suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.5
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    • pp.294-299
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    • 2000
  • Hairy vetch (Vicia villosa Roth) winter annual is very effective on reducing chemical nitrogen fertilizer for subsequent com by fixed organic green manure nitrogen fixed during hairy vetch growth. In this experiment, hairy vetch produced above-ground dry matter of 5 ton/ha, nitrogen yield 200 kgN/ha, at com planting on the average during 1997 and 1998. Changes in com yield and nitrogen uptake for two years were investigated after application of nitrogen fertilizer 0, 60, 120, 180, 240 kgN/ha on plot of winter fallow and hairy vetch green manure, respectively. Nitrogen status such as ear-leaf N%, SPAD value at silk and dough stage, and com yield decreased in proportion to reduction of nitrogen fertilizer at winter fallow, but nitrogen status and yield of com were not different among nitrogen fertilizer rate at hairy vetch green manure. Com yield (total dry matter) at 0 kgN/ha plot of hairy vetch was 22, 20 ton/ha in 1997, 1998, respectively and com could produce more dry matter 9, 13 ton/ha by hairy vetch green manure compared with winter fallow under the condition of no nitrogen fertilizer in 1997, 1998, respectively. Com yield (total dry matter) at 60kgN/ha of hairy vetch green manure was higher than that of high N fertilizer rate such as 180, 240 kgN/ha of winter fallow. Nitrogen uptake of com at plot of hairy vetch-no nitrogen fertilizer slightly decreased than at plot of hairy vetch - nitrogen fertilizer, but com absorbed more nitrogen of 141, 159 kgN/ha by hairy vetch green manure compared with winter fallow under no nitrogen fertilizer condition in 1997, 1998, respectively. Nitrogen fertilizer reduction for com by hairy vetch green manure was 149, 161kgN/ha in 1997, 1998, respectively. Still more, com could absorb more soil nitrogen by nitrogen fertilizer 60kgN/ha of hairy vetch green manure than by high nitrogen fertilizer such as 180, 240 kgN/ha at winter fallow. It is concluded that nitrogen fertilizer for corn could be reduced by winter cultivation and soil incorporation of hairy vetch at com planting.

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Effect of Nitrogen and Age of Alfalfa ( Medicago sativa L. ) Seeding on Growth and Nodulation When Growth under a Cool Environment (저온하에서 질소시비가 근류균을 접종한 Alfalfa의 생육부위에 미치는 영향에 관하여)

  • ;E. H. Jensen
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.6 no.3
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    • pp.151-156
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    • 1986
  • There are differences in recommendations in the USA as to whether nitrogen fertilizer should be applied when establishing alfalfa (Medicago sativa L). The reason for not applying nitrogen is because some researchers found the addition of nitrogen reduced nodulation of alfalfa plants. A replicated experiment was conducted under controlled environmental conditions at the University of Nevada-Reno, Reno, Nevada, USA, to determine the effects of nitrogen application on seedling growth and nodulation of alfalfa when grown in a cool environment. A sterile sand was used in the growing media to which a complete nutrient solution minus nitrogen was applied volumetrically to each pot daily. Half of the pots received $NH_4NO_3$, at the rate of 11.2 kg/ha, at seeding and two and four weeks after planting, giving a total nitrogen application rate of 33.6 kg/ha. Rhizobia inoculant (R-12) consisted of a mixture of strains 171-15a. 1682c and 80 PI 265 of Rhizobium meliloti. Inoculant was applied to the seeds prior to planting and to the sand media at two and four weeks after seeding. Twenty seeds were planted in pots 14.0 cm in diameter and 11.5 cm deep. Seedlings were thinned after emergence to ten plants per pot. They were grown in a controlled environment chamber with a 16-hour light period. Soil temperatures at 6 cm depth ranged from $5.7^{\circ}C\;to\;21.5^{\circ}C$ and had a daily mean of $16.2^{\circ}C$ Plants were harvested at weekly intervals for seven weeks at which time root, shoot and total length, dry weight, volume and number of nodules per plant were determined. Root, shoot and total length were not affected by nitrogen fertilizer. However, application of nitrogen increased the size of the seedlings as determined by dry weight and volume when compared to plants which were not fertilized. This indicates that rhizobia did not fix enough atmospheric nitrogen to promote good growth. Nitrogen application resulted in significantly more nodules per plant. The effect of nitrogen fertilizer became more apparent as the plant became older. Results of this experiment show there are benefits from applying nitrogen at a low rate when establishing alfalfa under a cool environment.

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Construction of High-Pressure Pressurized Liquid Nitrogen Supply Facilities (고압의 가압식 액체질소 공급 설비 구축)

  • Shin, Minkyu;Oh, Jeonghwa;Kim, Seokwon;Ko, Youngsung;Chung, Yonggahp
    • Journal of Aerospace System Engineering
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    • v.14 no.5
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    • pp.26-32
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    • 2020
  • In this study, a facility was constructed to supply liquid nitrogen to simulate combustion instability in a liquid rocket combustor. The pressurization and supply performances were predicted and verified through different experiments. The liquid nitrogen supply system was composed of a pressurized supply system, and a dome regulator was used to adjust the pressure of the pressurant. A cavitation venturi was used to control the mass flow rate of liquid nitrogen. The condition of liquid nitrogen supply was a mass flow rate of 2.55 kg/s and the venturi inlet pressure was above 100 bar. Based on the initial experiment, it was observed that the predicted amount of the pressurant was not sufficiently supplied and the target pressure was not supplied due to a drop in tank pressure. Through the modification of the established facilities, the target mass flow rate was successfully supplied and the cryogenic liquid nitrogen supply facility was verified.