• Title/Summary/Keyword: Nitrogen components

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The analysis of useful components in Flammulina velutipes fruit body, Flammulina velutipes mycelium and Cordyceps militaris mycelium (팽이버섯 자실체, 팽이버섯 균사체 및 동충하초 균사체의 유용성분 분석)

  • Kim, Yong-Doo;Kwak, Sang-Ho;Kim, Kyung-Je;Seo, Kyoung-Sun;Park, Tae-Young;Yu, Kang-Yeol;Jin, Seong-Woo
    • Journal of Mushroom
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    • v.12 no.3
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    • pp.193-200
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    • 2014
  • Flammulina velutipes fruit body, Flammulina velutipes mycelium and Cordyceps militaris mycelium were analyzed for their proximate composition, protein-bound polysaccharide, nucleic acid and amino acids. The content of ash and crude fiber in F. velutipes fruit body were higher than F. mycelium and C. militaris mycelium. C. militaris mycelium showed the highest crude fat content while F. velutipes fruit body had lowest. Nitrogen free extract content of the samples varied from 56.8% in F. velutipes fruit body to 61.9% in F. velutipes mycelium. The compositions of total protein and total free sugars of protein-bound polysaccharide were found to be significant differences for all samples. Nucleic acid related compounds were identified the 5'-GMP, 5'-XMP, 5'-IMP in all samples. The content of total nucleic acids were high in the orders of F. velutipes myclial (286.71 mg%), F. velutipes fruit body(187.36 mg%) and C. militaris mycelial(76.85 mg%). The highest content of 5'-GMP was found in F. velutipes fruit body. The most nucleic acid of F. velutipes mycelial and C. militaris mycelial were the 5'-XMP. As for the analysis of total amino acids, seventeen amino acids were identified by HPLC and the major amino acid was glutamic acid in all samples. The content of total amino acids were high in the orders of F. velutipes fruit body(19,919 mg%), F. velutipes mycelium(19,018 mg%) abd C. militaris mycelium(18,965 mg%). We determined the developing new food product such as amino acid drink and amino acid containing food using extracts of Flammulina velutipes fruit body, Flammulina velutipes mycelium and Cordyceps militaris mycelium.

Nutritional Effects of Paper Board Sludge on the Garlic(Allium sativum L.) (마늘에 대(對)한 판지(板紙) SLUDGE의 영양학적(營養學的) 연구(硏究))

  • Kim, Moon-Kyu;Chang, Ki-Woon;Choi, Woo-Young;Lee, Chang-Jun
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.3
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    • pp.208-213
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    • 1990
  • The parer board sludge (PBS) itself and compost sludge manure (CSM) made of sawdust, fowl droppings, and urea to the PBS were treated to garlic to determine the effects of growing characters, yield components, and nutritional compounds in the plants. 1. The differences of the rate of missing plants were not found between control and treatments. 2. Plant height of the garlic increased in the treatments of PBS and CSM rather than control. Numbers of leaf, and width of the largest leaf and stem did not have any distinct tendency to change. 3. Total weight and weight of the above ground part were highest in the treatment of 1,600kg/10a PBS, and CS-2 treatment mixed with high portion of additives. The weight of underground part and yield increased with the increase of paper board sludge, and highest in CS-2 treatment. 4. The contents of nitrogen in the plant were higher in the treatments of PBS and CSM than control. And CSM treatments had generally higher concentrations than PBS treatments. 5. In conclusion, PBS itself had nutritional effects. The PBS could be used to some crops after fermentation with adequate additives.

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Studies on Production of Heteropolysaccharide by Mutant of Xanthomonas malvacearum (Xanthomonas malvacearum 돌연변이주(突然變異株)의 Heteropolysaccharide 생산성(生産性)에 관(關)하여)

  • Lee, Ke-Ho;Kim, Mi-Sun;Park, Chan-Yung
    • Applied Biological Chemistry
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    • v.30 no.1
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    • pp.77-87
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    • 1987
  • The mutant with high productivity, X. malvacearum SNUF 560-6, was acquired from the X. malvacearum SNUF 560 with low productivity by UV-light irradiation. It was preserved is lyophilized stock culture and it was transferred to PDA slant to maintain viability fortnightly. Fermentations were started by retransfering to MY agar slant from PDA stok culture. The experiments for optimal xanthan gum production were studied in a chemically defined medium. Of the carbon and nitrogen sources tested, 0.4% sucrose medium and 10mM glutamic acid medium yielded the highest xanthan gun production respectively. The addition of 10g/l succinic acid stimulated xanthan gum production. Also 65mM $PO_4\;^{-3}\;(12.6g/l\;KH_2PO_4)$ was effective on xanthan gum production. Finally, medium 1 and medium 2 which have high xanthan gum production potencies were achieved in this stud. The components of medium 1 and medium 2 were as follows: Medium 1 : sucrose 40g/l glutamate 10mM $PO_4\;^{-3}\;54mM\;(KH_2PO_4\;12.65g/l)$ Citrate 2g/l $MgSO_4{\cdot}7H_2O\;0.2g/l$ $H_3BO_3\;0.005/l$ ZnO 0.006/l $FeCl_2{\cdot}6H_2O\;0.0024g/l$ $CaCO_3\;0.02g/l$ Medium 2 : $Sucrose\;40g/l\;(NH_4)_2SO_4\;2g/l$ $PO_4\;^{-3}\;65mM\;(KH_2PO_4\;12.65g/l)$ Succinate 10g/l $MgSO_4{\cdot}7H_2O\;0.02g/l$ $H_3BO_3\;0.06g/l$ ZnO 0.006g/l $FeCl_2{\cdot}6H_2O\;0.0024g/l$ $CaCO_3\;0.02g/l$.

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Physiochemical property of edible tissues (sprout and root) of steam-treated reed (증자처리에 의한 갈대 식용가능 부위(어린순과 뿌리)의 이화학적 특성)

  • Kim, Sung-Tae;Huh, Chang-Ki;Kim, Su-Hwan;Kim, Yong-Doo
    • Food Science and Preservation
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    • v.22 no.4
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    • pp.482-489
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    • 2015
  • This study was performed for the comparison of the general components, minerals, amino acids, organic acids, free sugars, ascorbic acid, polyphenol content and DPPH free radical scavenging activity of steamed-treated reed (sprout and root) to those of raw reed. Moisture content of sprout and root of reed after steaming treatment decreased from 81.28% and 81.64% to 70.18% and 65.50%, respectively. Crude ash content was the highest in raw sprout and steam-treated root. Crude lipid content of raw sprout and root were almost similar. In addition, crude lipid content of steam-treated sprout was greater than that of root. Nitrogen free extract content of root was 2 times as high as that of sprout. Total free sugar contents of raw sprout and root increased from 1,311.39 mg% and 4,130.98 mg% to 1,157.79 mg% and 3,750.90 mg%, respectively, after steam treatment. Furthermore, the organic acid contents of sprout and root of reed after steam treatment were less than those of raw sprout and root. Calcium and potassium contents were the highest among others in both steam-treated and raw reed. Amino acid content of sprout was higher than that of root in both before and after steam treatment. Among the amino acids, serine content was the best presented in both before and after steam treated reed. Vitamin C content of steam-treated sprout and root of reed decreased from 61.74 mg% and 6.57 mg% to 4.54 mg% and 80.79 mg%, respectively. Total polyphenol content of sprout was greater than that of root in raw and steam-treated reed. DPPH free radical scavenging activity of ethanol extraction of root was greater than those of other extracts of root.

Studies on the Feeding Value of Some Leguminosae Tree Leaves (두과수엽(荳科樹葉)의 사료가치(飼料價値)에 관한 연구(硏究))

  • Kim, Jong Woo
    • Korean Journal of Agricultural Science
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    • v.2 no.2
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    • pp.399-404
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    • 1975
  • In order to study on the utility for feed of several leguminosae tree leaves, the leaves of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia were collected from several places of Daejon city and Daeduck-Kun at the different growing seasons. The leaf productivities, the change of the contents of chemical components on the different growing seasons. and the palatability trial were determined. The results were summarized as follows: 1. Leaf productivities of Amorpha fruticosa and Robinia pseudo acacia were higher than that of Lespedeza spp., and the highest production of Amorpha' fruticosa was showed in august and that of Robinia pseudo acacia was showed in september. 2. Crude protein contents of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia were determined to 12.6, 18.63 and 15.87% respectively and the protein contents of them showed gradual decrease as growing. 3. Crude fat contents of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia were determined to 9.00, 5.85 and 4.70% respectively, and they displayed gradual increase as growing, 4. Crude fiber contents of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia were determined to 14.97, 12.61 and 9.80% respectively, and they showed gradual increase as growing. 5. The content of nitrogen free extract of Robinia pseudo acacia showed higher than those of Lespedeza spp. and Amorpha fruticosa, and all of them displayed gradual decrease as growing. 6. Crude ash contents of Lespedeza spp., Amorpha fruticosa and Robinia pseudo acacia determined to 4.40, 4.98 and 5.27% respectively, and variable changes were appeared as growing. 7. The results of palatability trial for three leaves showed superior to the order of Lespedeza spp., Robinia pseudo acacia and Amorpha fruticosa, and the dried leaves were more favorable than green leaves.

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Classification of West Coastal Dune Vegetation and Comparison of the Inorganic Constituents for the Soil and Present Plants (서해안 해안사구식생의 유형분류와 사구토양 및 식물 무기성분 비교)

  • Shin, Hak Sub;Kim, Hye Jin;Han, Sang Hak;Ko, Seong Yeon;Kang, Hye Jin;Lee, Seo Hui;Lee, Chun Yong;Kim, Chan Beom;Bae, Young Tae;Shin, Jae Kwon;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.102 no.3
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    • pp.345-354
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    • 2013
  • To understand the community structures of the vegetation of the beaches and coastal dunes of Seocheon-gun, Boryeong-si, Anmyeon-do, Taean-gun in Chungcheongnam-do and Buan-gun in Jeollabuk-do at the west coast of South Korea, a plant sociological vegetation survey was carried out to make a classification of correlation vegetation communities, a cluster analysis and an inorganic component analysis on the soil and plant body. Regarding the vegetation of the coastal dunes, plant communities were classified into 31 communities by correlation dominant species, and as a result of a cluster analysis on the vegetation communities, they were classified into five vegetation types such as Calystegia soldanella community, Rosa rugosa community, Imperata cylindrica community, Vitex tend community and Elymus mollis community. The soil pH was highest in Seocheon area ($7.54{\pm}1.18$) while lowest in Buan area ($6.29{\pm}2.15$), and regarding inorganic components, the nitrogen content was highest in Robinia while lowest in Imperata cylindrica. The soil PH of vegetation I was highest (8.23) among the five vegetation types, and the organic matter was lowest (71.58). As a result of CCA ordination analysis to examine the correlation among environmental factors, Calystegia soldanella community, Rosa rugosa community and Imperata cylindrica community among the five vegetation types were distributed while they were not affected by soil environmental elements, but Vitex tend community and Elymus mollis community were distributed mainly at the places with high $K^+$ content in the soil.

Evaluation of Spent Mushroom Substrates as Food for White-spotted Flower Chafer, Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) (버섯 수확 후 배지의 흰점박이꽃무지 사료화 연구)

  • Lee, Seul Bi;Kim, Jong Won;Bae, Sung Mun;Hwang, Yeon Hyeon;Lee, Heung-Su;Lee, Byeong Jeong;Hong, Kwang Pyo;Park, Chung Gyoo
    • Korean journal of applied entomology
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    • v.57 no.2
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    • pp.97-104
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    • 2018
  • The larva of the white-spotted flower chafer, Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae), is known to have important medicinal properties, such as anti-cancer activity. However, the consumer market for the larvae is depressed because of its high production cost which needs to be reduced. This study was carried out to evaluate two spent mushroom substrates as food source for chafer larvae to improve the productivity and reduce production cost. The larvae were fed with spent substrates of king oyster mushroom (Pleurotus eryngii) (P-SMS) and shiitake mushroom (Lentinula edodes) (L-SMS), with a control of fermented oak sawdust. Effects of the diets on the development of P. brevitarsis seulensis and contents of minor nutrient components in the larvae produced were examined. In the P-SMS diet, the larval rearing period was shorter at 16.2 days, while the rate of larval weight gain and the cocoon weight were higher at 156.3% and 4.1 g, respectively, than that in the L-SMS diet. The pupation rate was 100% and the adult emergence was higher at 93.3% in the P-SMS diet than in the L-SMS diet with no difference to the control diet. In the P-SMS diet, the total nitrogen content during rearing was higher at 10.28% and the minor nutrient component, particularly Fe (145.8 mg/kg), was higher than that in other diets. The oviposition preference of adults showed no significant difference among the diets. Economic analysis showed that P-SMS costs approximately 667,960 Won less per 100 kg of larvae produced, compared to that of the control diet.

Food Component Characterization of Muscle around Pectoral Fin, Salmon Fillet Processing By-products (연어 Fillet 부산물인 가슴지느러미 부근 근육의 식품성분 특성)

  • Heu, Min-Soo;Lee, Dong-Ho;Kang, Kyung-Tae;Kim, Hyung-Jun;Yoon, Min-Seok;Park, Kwon-Hyun;Kim, Jin-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.1
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    • pp.70-75
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    • 2009
  • For the effective use of salmon processing by-products, the food components of muscle around pectoral fin (MAPF) were investigated and compared with those of salmon fillet muscle (SFM). The proximate composition of MAPF was 64.4% for the moisture, 17.2% for the crude protein, 16.2% for the crude lipid and 1.4% for the ash. The pH, volatile basic nitrogen (VBN) content and yield of MAPF were 6.73, 7.8 mg/100 g and 7.4 (g/100 g SFM), respectively. The trichloroacetic acid (TCA) soluble-N content of MAPF was 479 mg/100 g, which was lower than that (612 mg/100 g) of SFM. The Hunter value of MAPF was 54.86 for L value, 22.08 for a value, 22.41 for b value and 52.35 for ${\Delta}$E value, which were similar to those of SFM. The total content in amino acids of MAPF was 17.1 g/100 g, its major amino acids were aspartic acid, glutamic acid, leucine and lysine. The major fatty acids of MAPF were 16:0, 18:1n-9 and 18:2n-6, 20:5n-3 and 22:6n-3. No differences were found in the major fatty acids and the major amino acids between MAPF and SFM. The calcium and phosphorus contents of MAPF were 19.1 mg/100 g and 211.7 g/100 g, respectively. These results suggested that MAPF could be used as seafood resources.

Study on Total Carbohydrate and Dietary Fiber in Domestic Herbs and Chinese Herbs (국내산 및 중국산 십전대보탕 재료의 구조탄수화물과 식이섬유에 관한 연구)

  • Kim Ki-Chul;Kim Ji-Min;Kim Dae-Jin
    • Journal of Life Science
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    • v.15 no.1 s.68
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    • pp.49-54
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    • 2005
  • This study was conducted to determine the total carbohydrate (CHO), nitrogen free extract (NFE), non fiberous carbohydrate (NFC) and ditery fiber (NDF, neutral detergent fiber; TDF, total dietary fiber; IDF, insoluble dietary fiber; SDF, soluble dietary fiber) of 17 kinds of domestic and Chinese herbs. The samples were dried at 60t for 24 hrs and ground to pass a 0.5mm screen. The contents of NFE was ranged from $85.73\%\;to\;57.57\%$, NDF was ranged from $85.72\%\;to\; 29.74\%$, CHO was ranged from $92.68\%\;to\;63.01\%$, TDF was ranged from $87.33\%$to 17.21, IDF was ranged from $86.37\%\;to\;14.56\%$, SDF was ranged from $6.06\%\;to\;0.97\%$ on domestic herbs in dry matter basis. NFE was ranged from $95.33\%\;to\;35.24\%$, NDF was ranged from $71.48\%\;to\;29.74\%$, CHO was ranged from $78.77\%\;to\; 35.24\%$, TDF was ranged from $86.43\%\;to\;16.22\%$, IDF was ranged from $85.98\%\;to\;13.04\%$, SDF was ranged from $4.13\%\;to\;0.45\%$ on Chinese herbs in dry matter. The several components of domestic and Chinese herbs were not significantly of TDF and IDF, but SDF was significantly by tow-tailed test. The correlationship of several herbs were r=0.99 between TDF and IDF (p<0.01), r=0.51 between TDF and SDF (p<0.01)on domestic herbs. The correlationship of 10 kinds herbs r=0.99 between TDF and IDF (p<0.01), r=0.50 between TDF and SDF (p<0.01) on Chinese herbs, respectively.

Effects of Stocking Density and Lipopolysaccharide on Immune Organ Weights, Blood Biochemical Profiles and the mRNA Expression of Pro-inflammatory Cytokines in Chicks (닭에서 사육밀도 및 Lipopolysaccharide 투여가 면역장기 무게, 혈액 생화학적 성상 및 친염증 사이토카인 mRNA 발현에 미치는 영향)

  • Jang, In-Surk;Song, Min-Hye;Kim, Ha-Na;Moon, Yang Soo;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.43 no.3
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    • pp.149-157
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    • 2016
  • This study was performed to investigate the effects of the stocking density (standard stocking density (SSD, $495cm^2/bird$)) vs. high stocking density (HSD,245cm2/bird) and challenge with lipopolysaccharide (LPS, 5mg/kg BW) on the stress-related physiological indicators in chicks. There was a significant (p<0.05) decrease in body weight, but not in the weight of immune organs, between the SSD and HSD groups. The LPS group resulted in a significant (p<0.05) increase in the weights of the thymus and bursa of fabricius compared with the SSD group. Plasma biochemical components, including aspartate transaminase (AST), alanine transaminase (ALT), blood urea nitrogen, Ca, P, creatine kinase and uric acid, markedly (p<0.05) increased in the LPS birds, although no difference in these parameters was observed between the SSD and HSD birds. Furthermore, the birds challenged with LPS showed a significant (p<0.05) increase in the plasma corticosterone level, although this hormone did not differ between the SSD and HSD groups. In the mRNA expression of pro-inflammatory cytokines, hepatic $IL-1{\beta}$, IL-6 and iNOS in the LPS group significantly (p<0.05) increased compared with those in the SSD group. Thymic mRNA expression of $IL-1{\beta}$, IL-6 and IL-18 in the LPS group also significantly (p<0.05) increased compared with those in the other groups. In addition, mRNA expression of $IL-1{\beta}$ in the bursa of fabricius of the LPS group increased (p<0.05) without affecting the other cytokines. Under high stocking density, thymic $IL-1{\beta}$ was the only cytokine that was up-regulated compared with the SSD group. In conclusion, an acute stress induced by LPS challenge profoundly affected immune organ weight, blood biochemical profiles and pro-inflammatory cytokine expression, while chronic stress did not markedly affect biochemical and immunological parameters, suggesting that chicks under high stocking density could be adapted to prolonged stressors.