• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1267-1272
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• 2005

The effect of various moisture contents of fresh forage on the change in nitrogen (N) fractions, in vitro ruminal N degradability, and the subsequent N utilization of silage in sheep were evaluated. Orchardgrass (Dactylis glomerata L.) with high (HM, 76%), medium (MM, 65%) and low (LM, 40%) moisture contents were ensiled into silos of 120 L capacity for 120 days. A nitrogen balance trial was conducted using a 4${\times}$4 Latin square design consisting of four dietary treatments (i.e. fresh forage, HM, MM and LM silages) and four wethers. With respect to N fractions, fraction 1 (buffer solution soluble N), fraction 2 (buffer solution insoluble N-neutral detergent insoluble N), fraction 3 (neutral detergent insoluble N-acid detergent insoluble N), and fraction 4 (acid detergent insoluble N) were determined. The proportion of fraction 1 in silages tended to decrease, while the in vitro ruminal degradability of insoluble N increased (p<0.05) with lower moisture contents at ensiling. Consequently, nitrogen utilization in sheep tended to improve as the moisture content of ensiled grass was decreased, with a negative correlation (p<0.01) between urinary N and the in vitro ruminal degradability of insoluble N. The averaged N retentions for HM, MM, and LM silage treatments were 59, 73 and 79% of that for fresh forage, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제29권10호
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• pp.1416-1423
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• 2016

This study was designed to investigate the effect of grape pomace powder (GPP), mangosteen peel powder (MPP) and monensin on feed intake, nutrients digestibility, microorganisms, rumen fermentation characteristic, microbial protein synthesis and nitrogen balance in dairy steers. Four, rumen fistulated dairy steers with initial body weight (BW) of $220{\pm}15kg$ were randomly assigned according to a $4{\times}4$ Latin square design to receive four treatments. The treatments were as follows: T1 = control, T2 = supplementation with monensin at 33 mg/kg diet, T3 = supplementation with GPP at 2% of dry matter intake, and T4 = supplementation with MPP at 30 g/kg diet. The steers were offered the concentrate diet at 0.2% BW and 3% urea treated rice straw (UTRS) was fed ad libitum. It was found that GPP supplemented group had higher UTRS intake and nutrient digestibility in terms of neutral detergent fiber and acid detergent fiber than those in control group (p<0.05). Ammonia nitrogen ($NH_3-N$) and blood urea-nitrogen concentration were higher in monensin, GPP and MPP supplemented groups (p<0.05). Total volatile fatty acids and propionate in the GPP group were higher than those in the control group (p<0.05) while acetate concentration, and acetate to propionate ratio were decreased (p<0.01) when steers were supplemented with GPP, monensin, and MPP, respectively. Moreover, protozoal populations in GPP, MPP, and monensin supplementation were significantly lower than those in the control group (p<0.05), while cellulolytic bacterial population was significantly higher in the control group (p<0.05). Nitrogen retention, microbial crude protein and efficiency of microbial nitrogen synthesis were found significantly higher in steers that received GPP (p<0.05). Based on this study it could be concluded that the GPP has potential as an alternative feed supplement in concentrate diets which can result in improved rumen fermentation efficiency, digestibility and microbial protein synthesis in steers fed on treated rice straw.

• Asian-Australasian Journal of Animal Sciences
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• 제29권5호
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• pp.689-694
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• 2016

The objective of the current study was to evaluate the effect of supplementation of either L-methionine (L-Met) or DL-methionine (DL-Met) to diets of starter pigs on nitrogen (N) balance, metabolism, and serum amino acid profile. Eighteen crossbred ($Duroc{\times}Landrace{\times}Yorkshire$) barrows weighing $15.45{\pm}0.88kg$ were randomly allotted to 1 of 3 diets with 6 pigs per treatment. The diets included a basal diet (Met-deficient diet) containing 0.24% standardized ileal digestibility Met with all other essential nutrients meeting the pig's requirements. The other two diets were produced by supplementing the basal diet with 0.12% DL-Met or L-Met. The experiment lasted for 18 days, consisting of a 13-day adaptation period to the diets followed by a 5-day experimental period. Pigs were fed ad libitum and free access to water throughout the experiment. Results showed that the supplementation of either L-Met or DL-Met improved N retention, and serum methionine concentration, and decreased N excretion compared with basal diet (p<0.01). The N retention of pigs fed diets supplemented with the same inclusion levels of DL-Met or L-Met were not different (p>0.05). In conclusion, on equimolar basis DL-Met and L-Met are equally bioavailable as Met sources for starter pigs.

• 한국식품영양과학회지
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• 제25권2호
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• pp.225-231
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• 1996

본 연구는 한국인이 사용하는 식이성 단백질의 소화 흡수율 체내 평형에 관한 자료를 얻기 위하여 국민학교 남자아동 8명, 여자아동 7명을 대상으로 4주간 평상시와 유사한 식단과 생활양식을 유지시키면서 각 대상자들이 섭취하는 동량의 모든 음식물과 배설한 대변 및 소변을 수거하여 질소 함량을 측정하므로서 1일 1인당 단백질 섭취량과 배설량을 측정하였고 이로부터 외견적 소화흡수율과 체내 평형상태를 구하였다. 단백질 섭취량은 남자아동은 43.6~61.6g 범위로 평균 $52.3\pm1.2g이었으며$ 여자아동은 34.6~50.6g 범위로 평균 $43.1\pm1.3g이었다.$ 대변중 단백질 배설량은 남자아동은 7.3~11.1g 범위로 평균 $9.2\pm0.3g이었으며$ 여자아동은 6.7~8.9g 범위로 평균 $7.8\pm0.2g이었다.$ 단백질 흡수율은 남자아동은 79.2~85.6%범위로 평균 $81.9\pm0.6%이었으며$ 여자아동은 79.6~84.5% 범위로 평균 $81.3\pm0.4%로$ 상호 유사하였으며 이들은 다른 연령층을 대상으로한 단백질 흡수율(80~85%)과 비슷하였다. 소변으로의 1일 질소 배설량은 남자아동은 4.4~5.0g 범위로 평균 $5.3\pm0.1g이었으며,$ 여자아동은 3.1~5.3g 범위로 평균 $3.9\pm0.2g이었으며$ 대변 및 소변으로의 질소 배설량을 감안한 질소 평형 상태는 남자아동은 +1.0~2.6g의 범위로 $1.60\pm0.1g의$ 양의 평형이었으며 여자아동은 1.4~2.1g의 범위로 평균 $1.7\pm0.1g의$ 양의 평형이었다.

• Nutrition Research and Practice
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• 제3권3호
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• pp.200-207
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• 2009

This study examined the effects of com gluten (CG) and its hydrolysate consumptions on weight reduction in rats fed a high-fat diet. Eight-month-old male Sprague-Dawley rats (n=40) were fed a high-fat diet (40% calorie as fat) for 4 weeks. They were then randomly divided into four groups and fed the isocaloric diets with different protein sources for 8 weeks. The protein sources were casein (control group), intact CG (CG group), CG hydrolysate A (CGHA group, 30% of protein as peptides and 70% as free amino acids) and CG hydrolysate P (CGHP group, 93% of protein as peptides and 7% as free amino acids). Body weight gain, adipose tissue weights, nitrogen balance, absorptions of energy, protein and fat, lipid profiles in plasma, liver and feces and hepatic activities of camitine palmitoyl transferase (CPT), fatty acid synthase (FAS), malic enzyme (ME) and glucose-6-phosphate dehydrogenase (G6PDH) were assessed. The CGHA diet had the highest amount of BCAAs, especially leucine, and most of them existed as free amino acid forms. The CGHA group showed significant weight reduction and negative nitrogen balance. Protein absorption and apparent protein digestibility in the CGHA group were significantly lower than those in other groups. Adipose tissue weights were the lowest in the CGHA group. Activity of CPT tended to be higher in the CGHA group than in other groups and those of FAS, ME and G6PDH were significantly lower in the CGHA group than in other groups. In conclusion, the CGHA diet which had relatively high amounts of free amino acids and BCAAs, especially leucine, had a weight reduction effect by lowering adipose tissue weight and the activities of FAS, ME and G6PDH in experimental animals, but it seemed to be a negative result induced by lowering protein absorption, increasing urinary nitrogen excretion and protein catabolism.

• Asian-Australasian Journal of Animal Sciences
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• 제17권5호
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• pp.688-692
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• 2004

Fifty four crossbred gilts of 26.38${\pm}$0.85 kg body weight and 25 weeks of age were randomly divided into three groups, having six replicates of three animals each and were assigned to one of the three dietary treatments containing corn, wheat bran and rice bran (RB) at 35, 47 and 0% in $R_1$; 17.5, 23.5 and 41% in $R_2$ and 0, 0 and 82% in $R_3$. Soybean meal and fishmeal were used as source of protein at 10 and 6%, respectively, in all the rations. Average daily CP, DCP, DE and ME intake per kg $W^{0.75}$ and body weight gains were lowest (p<0.01) in gilts fed 82% RB ($R_3$), followed by $R_2$ and R1. Utilization of protein with respect to nitrogen balance and DCP conversion efficiency did not differ (p>0.05) between the treatment groups. All the gilts were in positive nitrogen balance. However, the excretion of dietary nitrogen and energy through faeces was higher (p<0.01) in gilts fed RB. However, the DE and ME conversion efficiency were higher (p<0.01) in gilts fed RB. Therefore, it can be concluded that the digestibility of energy and protein in diets containing rice bran goes on decreasing with the increasing level of fat and fibre, but the metabolizability of the energy and protein was better in pigs fed RB in the diet.

• 한국유기농업학회지
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• 제20권1호
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• pp.21-36
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• 2012

In this study, the assessment of livestock manure nitrogen loading for recycling farming in Yeoju-Gun carried out comparing manure units based on the cultivation areas and the N-amount of manure that are generated from livestock manure. Manure units (MU) are used in the permitting, registration, because they allow equal standards for all animals based on manure nutrient production. An MU is calculated by multiplying the number of animals by manure unit factor for the specific type of animal. The manure unit factor for MU determination was determined by dividing amounts of manure N produced 80kg N/year. In this study, manure unit by nitrogen concentration and amount of animal manure was calculated as follows: Hanwoo multiplied by 0.36, dairy cows multiplied by 0.8. swine multiplied by 0.105. The laying hens and broilers multiplied by 0.0079, 0.0049, respectively. The analysis of liquid manure unit per ha shows that the N loading by LMU is quite different by region. When it comes to nitrogen loading, the LMU per ha of cultivated land in excess of the N-amount was the highest in the Bukne-myeon province with 2.76 MU/ha, which is higher than the appropriate level. The Ganam-myeon province came next with 2.53 LMU. To be utilized as a valid program to build the environmentally friendly agricultural system, diverse measures shall be mapped out to properly determine manure units, evaluate N-loading and to properly manage their nutrient balance of each region.

• 한국토양비료학회지
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• 제50권5호
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• pp.325-335
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• 2017

This study was carried out for 30 days in aeration type and agitation type reactor to characterize organic matter decomposition and ammonia volatilization during the liquid composting of pig slurry, and organic matter and nitrogen removal rate through mass balance analysis was analyzed. In the aeration type reactor, the pH increased from 7.0 to 9.13, and TS 34.5%, VS 33.4%, $BOD_5$ 71.2%, $COD_{Cr}$ 62.3% and TOC 83.2% were removed. In addition, 44.6% of TN and 65.0% of ${NH_4}^+-N$ were removed. In the agitation type reactor, the pH increased from 7.0 to 8.10, and the removal rates of TS 0.9%, VS 0.5%, $COD_{Cr}$ 27.5%, $BOD_5$ 28.9% and TOC 41.3% were obtained. And TN and ${NH_4}^+-N$ showed removal rate of 25.3% and 29.2%, respectively. The first order kinetics constant related to $BOD_5$ degradation was $-0.039day^{-1}$ for aerobic liquid composting and $-0.013day^{-1}$ for agitated reactor. Nitrogen loss in aerobic liquid composting was about 2.3 times higher than that of agitated reactor, whereas FAN/TAN in aerobic liquid composting was about 7.9 times higher than that of agitation type reactor. Therefore, despite the low FAN/TAN in the agitation type reactor, the nitrogen loss rate was relatively high.

• 한국환경보건학회지
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• 제27권2호
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• pp.145-152
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• 2001

The purpose of this study was to determine nitrogen dioxide(NO$_2$) decay rate by reaction between NO$_2$ and interior materials in Korean residence. The results of this research could be helpful to choose the interior construction materials and to study on reduction of indoor air pollutants. The results of this research are as follows; For 30 residences in Seoul and Incheon from October 2000 to march 2001, the mean of infiltration rate was 0.70$\pm$0.44 ACH, and single-detached houses (7 houses) and apartments (19 houses) were 0.97$\pm$0.55 ACH and 0.61$\pm$0.34 ACH, respectively. The $CO_2$ decay followed approximately first-order process ($R^2$=0.97$\pm$0.02). There existed a statistic significance in filtration rate between houses built in 1980’s and built in 1990’s by t-test (p<0.02). Mean of NO$_2$ decay rates in 26 residence3s except 4 residences was 0.94$\pm$0.49hr$^{-1}$ , and also 0.86$\pm$0.49hr$^{-1}$ , 0.97$\pm$0.50hr$^{-1}$ in single-detached houses and apartments, respectively. Mean NO$_2$ decay rates in houses built in 1980’s were 0.78$\pm$0.37hr$^{-1}$ , 1.33$\pm$1.03hr$^{-1}$ , respectively. Nothing were showed statistical significance among indoor temperature, indoor humidity, and NO$_2$ decay rate. However, NO$_2$ decay rates had a tendency to increase by increase of temperature and humidity. Average volume/surface of participated houses was 0.55$\pm$0.07m and mean NO$_2$ deposition velocity was calculated as 1.46$\pm$0.59msec$^{-1}$ .

• 환경위생공학
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• 제12권2호
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• pp.1-12
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• 1997

This research aims to develop biofilm process for the nutrient removal of piggery wastewater. The developed process is the four stage anoxic-oxic biofilm process with recirculation of the final effluent. In summery, the results are as follows: 1. Nitrification in the piggery wastewater built up nitrite because of the high strength ammonia nitrogen. The nitrification of nitrobacter by free ammonia was inhibited in the total ammonia nitrogen loading rate with more than 0.2 kgNH$_{3}$-N/m$^{3}$·d. 2. The maximal total ammonia nitrogen removal rate was obtained at 22$\circ $C and without being affected by the loading rate. But total oxidized nitrogen production rate was largely affected by loading rate. 3. Autooxidation by the organic limit was a cause of the phosphorus release in the aerobic biofilm process. But the phosphorus removal rate was 90 percent less than the influent phosphorus volumetric loading rate of above 0.1 kgP/m$^{3}$·d. Therefore, the phosphorus removal necessarily accompanied the influent loading rate. 4. On the anoxic-oxic BF process, the total average COD mass balance was approximately 67.6 percent. Under this condition, the COD mass removal showed that the cell synthesis and metabolism in aerobic reactor was 42.8 percent and that the denitrification in anoxic reactor was 10.7 percent, respectively.