• The Korean Journal of Ecology
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• v.21 no.4
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• pp.329-335
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• 1998

The growth of Phytolacca L. grown under three light regimes at three nutrient concentrations was analyzed. The effect of shading treatment on plnat growth was greater than that of nutrient treatment. Plant dry mass increased more than 5-fold during 21 days under 33% and 100% irradiances, whereas that was strongly reduced under 8% irradiance. Net assimilation rate decreased with plant growth irrespective of light and nutrient treatments, though the highest net assimilation rate was shown under 100% irradiance. Under 33% irradiance leaf area in plants supplied with nutrient solution increased to such extent as to compensate reduction in net assimilation rate, whic maintains almost identical growth rate with that under 100% irradiance. The relatonship between total plant nitrogen and leaf nitrogen content was dependent on the growth irradiance. Moreover, leaf nitrogen and specific leaf weight were also changed depending on the light and nutrient conditions. Based on these findings, it is suggested that the adaptive characteristics of Pokeberry plant to light and nutrient conditions may contribute to rapid extension of Pokeberry habital in Korea rocently.

• The Korean Journal of Ecology
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• v.16 no.3
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• pp.261-273
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• 1993

The nitrogen assimilation rate of nitrogenous nutrients by reservior phytoplankton was masured in the in situ condition in the euphotic layer of Lakes Soyang, Chuncheon and Uiam located on the upper reaches of the North Han River System in August, 1983, Korea. The assimilation rate of ammonia, nitrate and urea nitrogen in surface water was 13, 2 and $13{\mu}g$ at. $N{\cdot}m^{-3}{\cdot}(12:10~18:15)^{-1}$ in Lake Soyang, 325, 27 and $59{\mu}g$ at. $N{\cdot}m^{-3}{\cdot}(12:30~18:30)^{-1}$ in Lake Chuncheon, and 174, 12 and $45{\mu}g$ at. $N{\cdot}m^{-3}{\cdot}(12:30~19:30)^{-1}$ in Lake Uiam. Ammonia and urea were perferntially utilized by reservoir phytoplankton. The dark/light ratios of nitrate assimilation were much lower than those of ammonia and urea assimilation of nitrate showed little contribution. The primary productuin was estimated as 59mg $C{\cdot}m^{-2}{\cdot}day^{-1}$ and 6.9mg $N{\cdot}m^{-2}{\cdot}day^{-1}$ in Lake Spyang, 217mg C{\cdot}m^{-2}{\cdot}day^{-1}$ and 26mg N{\cdot}m^{-2}{\cdot}day^{-1}$ in Lake Chuncheon, and 110mg C{\cdot}m^{-2}{\cdot}day^{-1}$ and 13mg N{\cdot}m^{-2}{\cdot}day^{-1}$ in Lake Uiam, with production ratios of 8.6, 8.4 and 8,4, respectively. The turnover time o ammonia and urea in the upper euphotic layer was 2 to 47 days and 4 to 38 days, respectively. Nitrate required much longer periods. In the euphotic layer of reservoirs, ammonia and urea played signigicant roles in the biogeoKDICical nitrogen metabolism.

• Korean Journal of Breeding Science
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• v.43 no.5
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• pp.349-359
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• 2011

Agriculture plays a vital role in the sustenance of human society and is the fundamental of developing economies. Nitrogen is one of the most critical inputs that define crop productivity. To ensure better value for investment as well as to minimize the adverse impacts of the accumulative nitrogen species in environment, improving nitrogen use efficiency of crop plants is of key importance. Efforts have been made to study the genetic and molecular biological basis as well as the biochemical mechanisms involved in nitrogen uptake, assimilation, translocation and remobilization in crops and model plants. This review gives an overview of metabolic, enzymatic, genetic and biotechnological aspects of nitrogen uptake, assimilation, remobilization and regulation. This review presents the complexity of nitrogen use efficiency and the need for an integrated approach combining physiology, quantitative trait genetics, system biology, soil science, ecophysiology and biotechnological interventions to improve nitrogen use efficiency.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.659-666
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• 2021

After Candida albicans, Candida glabrata is one of the most common fungal species associated with candidemia in nosocomial infections. Rapid acquisition of nutrients from the host is important for the survival of pathogens which possess the metabolic flexibility to assimilate different carbon and nitrogen compounds. In Saccharomyces cerevisiae, nitrogen assimilation is controlled through a mechanism known as Nitrogen Catabolite Repression (NCR). NCR is coordinated by the action of four GATA factors; two positive regulators, Gat1 and Gln3, and two negative regulators, Gzf3 and Dal80. A mechanism in C. glabrata similar to NCR in S. cerevisiae has not been broadly studied. We previously showed that in C. glabrata, Gln3, and not Gat1, has a major role in nitrogen assimilation as opposed to what has been observed in S. cerevisiae in which both factors regulate NCR-sensitive genes. Here, we expand the knowledge about the role of Gln3 from C. glabrata through the transcriptional analysis of BG14 and gln3Δ strains. Approximately, 53.5% of the detected genes were differentially expressed (DEG). From these DEG, amino acid metabolism and ABC transporters were two of the most enriched KEGG categories in our analysis (Up-DEG and Down-DEG, respectively). Furthermore, a positive role of Gln3 in AAA assimilation was described, as was its role in the transcriptional regulation of ARO8. Finally, an unexpected negative role of Gln3 in the gene regulation of ABC transporters CDR1 and CDR2 and its associated transcriptional regulator PDR1 was found. This observation was confirmed by a decreased susceptibility of the gln3Δ strain to fluconazole.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.44-48
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• 1999

Uptake, assimilation and translocation of nitrogen and dry matter assimilation and translocation in ten rice cultivars were observed in no-till direct-sown rice-vetch cropping system. There was a large degree of variation in N-uptake, grain yield, nitrogen translocation efficiency and dry matter assimilation and translocation in tested rice cultivars. Forty kg N/ha base, as compound fertilizer (21-17-21% of N-P-K) three weeks after sowing and 30 kg N/ha top-dressed at panicle initiation stage as in the form of (NH$_4$)$_2$$CO_2$ was applied. ‘Newbounet’, ‘Daesanbyeo’, and ‘Hwayeongbyeo’ showed higher translocation efficiency. The contribution of pre-heading dry matter assimilates to grain ranged from 33% to 99% of dry grain weight. Dry matter of ‘Calrose 76’ was lower than Newbounet but N content was higher in Calrose 76 than Newbonnet. By maturity, N content in vegetative parts declined considerably more than dry matter, vegetative and reproductive parts, N translocation efficiency, and N harvest index. Nitrogen translocation efficiency was greater in ‘Nonganbyeo’, Daesanbyeo, and Newbounet. Grain N concentration was positively correlated with N concentration or N content of the vegetative parts at heading in Nonganbyeo, ‘Dasanbyeo’, ‘Dongjinbyeo’, and Newbonnet. These results indicated that the greater amount of dry matter and N accumulated before heading stage, the higher translocation rates of dry matter to grain and the greater net losses at maturity.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.124-127
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• 2008

The behavior of Penicillium camembertii and Geotrichum candidum growing in submerged pure cultures on simple (glutamate) or complex (peptones) substrates as nitrogen and carbon sources and lactate as a second carbon source was examined. Similar to the behavior previously recorded on a simple substrate (glutamate), a clear differentiation between the carbon source and the energy source was also shown on peptones and lactate during P. camembertii growth, since throughout growth, lactate was only dissimilated, viz., used for energy supply by oxidation into $CO_2$, whereas peptides and amino acids from peptones were used for carbon (and nitrogen) assimilation. Because of its deaminating activity, G candidum preferred peptides and amino acids to lactate as energy sources, in addition to being assimilated as carbon and nitrogen sources. From this, on peptones and lactate, G candidum grew faster than P. camembertii (0.19 and 0.08 g/l/h, respectively) by assimilating the most readily utilizable peptides and amino acids; however, owing to its lower proteolytic activity, the maximum biomass was lower than that of P. camembertii (3.7 and 5.5 g/l, respectively), for which continuous proteolysis and assimilation of peptides were shown.

• Journal of Microbiology and Biotechnology
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• v.2 no.4
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• pp.255-259
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• 1992

The effect of nitrate, nitrite and ammonia as inorganic nitrogen sources on the modulation of nitrogen metabolism of Botryococcus braunii UTEX.-572 has been studied under aeration. The primary process in the regulation of nitrogen metabolism by this alga has the nitrate uptake system. This uptake of nitrate operation was immediately inhibited by the presence of 0.5 mM of ammonium and reversed by 0.2∼0.3 mM ammonium. When cell were exposed to 5 mM of ammonium for 24 hours the activity of nitrate reductase became inactive.

• Asian Journal of Turfgrass Science
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• v.10 no.2
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• pp.125-142
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• 1996

Crassulacean acid metaholism (CAM) was investigated in leaves and stems of the succulent $C_4$dicot Portulaca oleracea L. Under 14-hour days, stem tissues showed much greater fluctuation of acidity than leaf tissues. But leaf and stem tissues showed almost same CAM-like pattern of acid fluctuation under 8-hour days. Stem tissues of R oleracea grown under the naturai environment showed high CAM activity, but no CAM activity was seen in leaves of those plants. In the naturally growing plants, the rapid acidification was seen in intact stems at dawn, but defoliated stems showed only a gradual increase. RuBP carlboxylase activity was very high at 2:00 P.M. in both leaves and stems. However, its activity at 1:00 A.M. and 5:30 AM. was hardly detected. particularly, activity of PEP carboxylase in leaves was very high in the early morning, though that in stem tissues was little. These results indicate that $CO_2$ passed through open stomata at dawn may be assimilated by PEP carboxylase in leaves, and then $C_4$ products move to stems. The levels of nitrate concentration and of nitrate reductase were higher in stems than in leaves. The levels were also higher in the light than in the dark. It would be suggested that considerable amount of nitrate absorbed from roots ho assimilated in stems, and nitrate transferred to leaves via stem tissues be reduced there. Key words: Portalaca oleracea, Cactus, Photosynthesis, Nitrogen assimilation, Crassulacean acid metabolism (CAM).

• Journal of Plant Biotechnology
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• v.6 no.4
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• pp.259-264
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• 2004

The present study describes the ameliorating effect of $Ca^{++}\;on\;Cd^{++}$ toxicity on the germination, early growth of mungbean seedlings, nitrogen assimilation enzyme. s-nitrate reductase (NR), nitrite reductase (NIR), anti-oxidant enzymes (POD, CAT and SOD) and on the accumulation of hydrogen peroxide and sulphydryls. $Cd^{++}$ inhibited seed germination and root and shoot length of seedlings. While NR activity was down- regulated, the activities of NIR, POD and SOD were up- regulated with $Cd^{++}$ treatment. $Cd^{++}$ treatment also increased the accumulation of sulphydryls and peroxides, which is reflective of increased thiol rich proteins and oxidative stress. $Ca^{++}$ reversed the toxic effects of $Cd^{++}$ on germination and on early growth of seedlings as well as on the enzyme activities, which were in turn differentially inhibited with a combined treatment with calcium specific chelator EGTA. The results indicate that the external application of $Ca^{++}$ may increase the tolerance capacity of plants to environmental pollutants by both up and down regulating metabolic activities. Abbreviations: $Cd^{++}= cadmium,\;Ca^{++} = calcium$, NR= nitrate reductase, NIR=nitrite reductase, POD = peroxidse, SOD= superoxide dismutase, CAT= catalase, EGTA= ethylene glycol-bis( $\beta-aminoethyl ether$)-N,N,N,N-tetraacetic acid.

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.14-14
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• 2023