• Title/Summary/Keyword: Nitrogen and phosphorus

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Studies on the Physiological Chemistry of Seed Development in Ginseng Seed (인삼식물의 종자발육 과정에 있어서의 생리화학적 연구)

  • Hee-Chun Yang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.17
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    • pp.115-133
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    • 1974
  • This study was done on the metabolism of chemical components during the seed development of ginseng. The changes of the chemical components were inspected in the following periods: from the early stage of flower organ formation to flowering time, from the early stage of fruiting to maturity, during the moisture stratification before sowing. From flower bud forming stage to meiosis stage, the changes in the fresh weight, dry weight, contents of carbohydrates, and contents of nitrogen compounds were slight while the content of TCA soluble phosphorus and especially the content of organic phosphorus increased markedly. From meiosis stage to microspore stage the fresh and dry weights increase greatly. Also, the total nitrogen content increases in this period. Insolub]e nitrogen was 62-70% of the total nitrogen content; the increase of insoluble nitrogen seems to have resulted form the synthesis of protein. The content of soluble sugar (reducing and non-reducing sugar) increases greatly but there was no observable increase in starch content. In this same period, TCA soluble phosphorus reached the maximum level of 85.4% of the total phosphorus. TCA insoluble phosphorus remained at the minimum content level of 14.6%. After the pollen maturation stage and during the flowering period the dry weight increased markedly and insolub]e nitrogen also increased to the level of 67% of the total nitrogen content. Also in this stage, the organic phosphorus content decreased and was found in lesser amounts than inorganic phosphorus. A rapid increase in the starch content was also observed at this stage. In the first three weeks after fruiting the ginseng fruit grows rapidly. Ninety percent of the fresh weight of ripened ginseng seed is obtained in this period. Also, total nitrogen content increased by seven times. As the fruits ripened, insoluble nitrogen increased from 65% of the total nitrogen to 80% while soluble nitrogen decreased from 35% to 20%. By the beginning of the red-ripening period, the total phosphoric acid content increased by eight times and was at its peak. In this same period, TCA soluble phosphorus was 90% of total phosphorus content and organic phosphorus had increased by 29 times. Lipid-phosphorus, nucleic acid-phosphorus and protein-phosphorus also increased during this stage. The rate of increase in carbohydrates was similar to the rate of increase in fresh weight and it was observed at its highest point three weeks after fruiting. Soluble sugar content was also highest at this time; it begins to decrease after the first three weeks. At the red-ripening stage, soluble sugar content increased again slightly, but never reached its previous level. The level of crude starch increased gradually reaching its height, 2.36% of total dry weight, a week before red-ripening, but compared with the content level of other soluble sugars crude starch content was always low. When the seeds ripened completely, more than 80% of the soluble sugar was non-reducing sugar, indicating that sucrose is the main reserve material of carbohydrates in ginseng seeds. Since endosperm of the ripened ginseng seeds contain more than 60% lipids, lipids can be said to be the most abundant reserve material in ginseng seeds; they are more abundant than carbohydrates, protein, or any other component. During the moisture stratification, ginseng seeds absorb quantities of water. Lipids, protein and starch stored in the seeds become soluble by hydrolysis and the contents of sugar, inorganic phosphorus, phospho-lipid, nucleic acid-phosphorus, protein phosphorus, and soluble nitrogen increase. By sowing time, the middle of November, embryo of the seeds grows to 4.2-4.7mm and the water content of the seeds amounts to 50-60% of the total seed weight. Also, by this time, much budding material has been accumulated. On the other hand, dry stored ginseng seeds undergo some changes. The water content of the seeds decreases to 5% and there is an observable change in the carbohydraes but the content of lipid and nitrogen compounds did not change as much as carbohydrates.

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Nitrogen and Phosphorus Content Changes in Paddy Soil and Water As Affected by Organic Fertilizer Application

  • Lee, Kyung-Do;Lee, Kyeong-Bo;Gil, Geun-Hwan;Song, In-hong;Kang, Jong-Gook;Hwang, Seon-Woong
    • Korean Journal of Environmental Agriculture
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    • v.30 no.1
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    • pp.1-8
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    • 2011
  • BACKGROUND: With increasing public awareness to environment-friendly agriculture, many efforts have been run to develop organic farming technologies in Korea as of late 90s. The objective of this study was to investigate the effects of different organic farming practices on soil chemical properties and water quality in paddy fields. METHODS AND RESULTS: Total nitrogen (TN) and total phosphorus (TP) were monitored for a two-year period (2006 to 2007) from the study organic paddy fields located in Wan-ju, Jeonbuk Province in Korea. TN and TP of organic paddy water were gradually increased for 2~3 weeks after organic manure application and then gradually decreased afterward. The overall variation of TP in the paddy fields was much greater than that of TN. The phosphorus content in organic paddy field appeared to increase with the organic farming period. CONCLUSION(s): This indicates that long-term organic farming is likely to cause phosphorus accumulation in soils and increase vulnerability to rainfall runoff. Thus, appropriate phosphorus management needs to be implemented, particularly, to reduce excessive phosphorus supply owing to nitrogen-based determination of organic manure application amount.

Comparison of Phosphorus and Nitrogen Removal Characteristics between A2/O and Modified Phostrip Processes (A2/O공정과 수정 Phostrip공정과의 질소 및 인제거 특성비교)

  • Kim, Kwang-Soo;Kim, I-Tai
    • Journal of Korean Society on Water Environment
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    • v.21 no.6
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    • pp.664-668
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    • 2005
  • An experimental study for the comparison of nitrogen and phosphorus removal efficiencies between $A^2/O$ and modified Phostrip (M-Phostrip process) were carried out with bench-scale reactors. In case of nitrogen removal efficiencies both of processes showed similar ones when influent organic loadings were high. However, M-phostrip process was more effective than $A^2/O$ at low organic loadings. This is why M-phostrip process consumes the whole mass of influent organics as a carbon sources for denitrification in anoxic reactor but the anoxic reactor of $A^2/O$ process utilizes the residual carbon followed by consumming a part of influent carbon for phosphorus release in anaerobic reactor. $A^2/O$ process required the influent COD/T-P and COD/TKN ratios were more than 56 and 10, respectively, to take place the phosphorus release in anaerobic process and phosphorus uptake in oxic process. However, the luxury uptake of phosphorus in M-phostrip process was not affected by influent COD/T-P and COD/TKN ratios and the adverse effect of nitrate in return sludge introduced to the p-stripper from the 2nd clarifier was not significant due to the configurational advantage of the p-stripper.

Reaction Characteristics of Piggery Wastewater for Biological Nutrient Removal (생물학적 영양염류 제거를 위한 돈사폐수의 반응 특성)

  • 한동준;류재근;임연택;임재명
    • Journal of environmental and Sanitary engineering
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    • v.13 no.1
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    • pp.44-56
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    • 1998
  • This study was performed to investigate the reaction characteristics of piggery wastewater for biological nutrient removal. The reaction characteristics were discussed the fraction of organics, the behavior of nitrogen, nitrification, denitrification, and the behavior of phosphorus. The fraction of readily biodegradable soluble COD was 11-12 percent. The ammonia nitrogen was removed via stripping, nitrification, autotrophic cell synthesis, and heterotrophic cell synthesis. The removal percents by each step were 12.1%, 68.9%, 15.0%, and 4.0%, respectively. Nitrification inhibition of piggery wastewater was found to occur at an influent volumetric loading rate over 0.2 NH$_{3}$-N kg/m$^{3}$/d. Denitrification rates were the highest in the raw wastewater and the lowest in the anaerobic effluent. The denitritation of piggery wastewater came out to be possible, and the rate of organic carbon consumption decreased about 10 percent. The phosphorus removed was released in the form of ortho-p in the aerobic fixed biofilm reactor, it was caused by autooxidation. The synthesis and release of phosphorus were related to the ORP and the boundary value for the phase change was about 170mV. In the synthesis phase, the phosphorus removal rate per COD removed was 0.023mgP$_{syn}$/mgCOD$_{rem}$. The phosphorus contents of the microorganism were 4.3-6.0% on a dry weight basis.

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Phosphorus and Nitrogen Rate Effects to a Newly Seeded Kentucky Bluegrass (질소와 인산의 시비량이 새로 조성된 Kentucky Bluegrass에 성장에 미치는 영향)

  • Lee, Sang-Kook
    • Asian Journal of Turfgrass Science
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    • v.25 no.2
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    • pp.217-222
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    • 2011
  • Professional turfgrass applicators have reduced or eliminated phosphorus from their fertilization programs based on the assumption that soil phosphorus levels are supplying adequate amounts of phosphorus to the turfgrass. The previous researchers found that there were no P effects for turfgrass growth especially for mature turf. No effects may result from high P level in heavy thatch layer. The research was conducted for one year to investigate the effects of phosphorus fertilization programs on turfgrass performance, and monitor soil and plant tissue nutrient levels to determine the impact of the programs on a newly seeded Kentucky bluegrass. The nitrogen treatments were 20, 30 and $40g\;m^{-2}\;yr^{-1}$. The low, medium, and high nitrogen treatments were applied over 2, 4 and 6 applications, respectively. Nitrogen was applied using a formulation containing 30% of slow and 70% of fast release nitrogen sources that are representative of typical home lawn fertilizers. The phosphorus treatments were 0, 10 and $20g\;m^{-2}\;yr^{-1}$. Phosphorus was applied according to the application schedule for the nitrogen treatments. Kentucky bluegrass was seeded in May, 2010. The thickness of thatch layer was less than 1 cm and the first treatment was applied to Kentucky bluegrass in April, 2011. The low N rate treatment had acceptable color and quality ratings without high clipping yields. The high N rate treatment consistently had the highest color and quality ratings but also had very high clipping yields in comparison to the low and medium N rate treatments. Although there are significant differences in tissue P, Overall, there was no effect of phosphorus on color, quality, or clipping weights.

Influence of Aeration Cycle on Nitrogen and Phosphorus Removal in Two-Stage Intermittent Aeration System (2단 간헐폭기 시스템에서 aeration cycle이 질소 및 인 제거에 미치는 영향)

  • Jeong, Myoung-Sun;Lee, Jun-Ho;Seo, Kwang-Bum;Kim, Yeong-Kwan
    • Journal of Industrial Technology
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    • v.23 no.A
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    • pp.193-197
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    • 2003
  • This bench-scale research investigated the aeration cycle(on/off) as the controlling factors for nitrogen and phosphorus removal in a 2-stage, intermittent aeration process. At this experiment, the aeration cycle time(air-on/air-off) was 30min/30min, 60min/60min, 90min/90min. Organic matter removal was observed more than 90% regardless of the aeration cycle and phosphorus removal was relatively high when the aeration cycle time was 60min/60min On the other hand. For all of the aeration cycle, TN removal was appeared less than 55%. This result was probably due to the limitation of the external substrate for heterotrophic nitrification and aerobic denitrification.

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Biological Nitrogen and Phosphorus Removal Characteristics on Organic Material and Nitrate Loadings in SBR Process (연속회분식반응조에서 유기물 부하와 질산염농도에 따른 생물학적 질소 및 인 제거 특성)

  • Kim, I-Tae;Lee, Hee-Ja;Kim, Kwang-Soo;Bae, Woo-keun
    • Journal of Korean Society on Water Environment
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    • v.20 no.6
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    • pp.571-576
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    • 2004
  • Since anaerobic/anoxic/oxic process, which is a typical mainstream biological nitrogen and phosphorus removal process, utilizes influent organic matter as an external carbon source for phosphorus release in anaerobic or anoxic stage, influent COD/T-P ratio gives a strong influence on performance of phosphorus removal process. In this study, a bench scale experiment was carried out for SBR process to investigate nitrogen and phosphorus removal at various influent COD/T-P ratio and nitrate loadings of 23~73 and 1.6~14.3g $NO_3{^-}-N/kg$ MLSS, respectively. The phosphorus release and excess uptake in anoxic condition were very active at influent COD/T-P ratios of 44 and 73. However, its release and uptake was not obviously observed at COD/T-P ratio of 23. Consequently, phosphorus removal efficiency was decreased. In addition, the phosphorus release and uptake rate in anoxic condition increased as the nitrate loading decreased. Specific denitrification rate had significantly high correlation with organic materials and nitrate loadings of the anoxic phase too. The rate of phosphorus release and uptake in the anoxic condition were $0.08{\sim}0.94kg\;S-P/kg\;MLSS{\cdot}d$ and $0.012{\sim}0.1kg\;S-P/kg\;MLSS{\cdot}d$, respectively.

Nitrogen and Phosphorus Dynamics in an Salt Marsh in the Nakdong River Estuary (낙동강 하구 염습지 식물군락의 질소 및 인의 동태)

  • Kim Joon-Ho;Hyeong-Tae Mun;Byeong;Kyung-Je Cho
    • The Korean Journal of Ecology
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    • v.12 no.1
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    • pp.1-7
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    • 1989
  • We studied primary production, nitrogen and phosphorus dynamics in a salt marsh of Okryutung at Nakdong River estuary. The standing biomass in Phragmites longivalvis, Carex scabrifolia and Zoysia sinica stand was $5.48kg/\textrm{m}^2,{\;}1.94kg/\textrm{m}^2{\;}and{\;}1.95kg/\textrm{m}^2$, respectively. The peak above-ground biomass in each stand was $1.99kg/\textrm{m}^2,{\;}0.74kg/\textrm{m}^2{\;}and{\;}1.03kg/\textrm{m}^2$, respectively. Soil nitrogen decreased from the onset of growing seson till July, and then increased. Seasonal patterns of soil phosphorus were different from stand to stand. Nitrogen concentrations of above-ground plant tissus were quite different among the plant species at the very beginning of the growing season, however, they became similar as the plants grow. Seasonal pattern of phosphrous in C. scabrifolia roots was quite different from those other two species. Nitrogen absorbed by plants during season in P. longivalvis, C. scabrifolia and Z. sinicia stand was 224kg/ha, 111kg/ha, 156kg/ha, respectively. Phosphorus taken up by plants was 22kg/ha, 29kg/ha and 21kg/ha, respectively. Because the vascular plants growing at salt marshes can immobilize large quantities of nitrogen and phosphorus, salt marsh vegetation can be sued for preventing the pollution of coastal sea water.

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Biological Nutrient Removal by Enhancing Anoxic Phosphate Uptake (무산소 조건에서의 인섭취를 이용한 생물학적 영양염류 제거)

  • Lee, Dae Sung;Jeon, Che Ok;Park, Jong Moon
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.5
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    • pp.861-867
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    • 2000
  • The feasibility of simultaneous phosphorus and nitrogen removal by enhancing anoxic phosphorus uptake was investigated in a sequencing batch reactor (SBR). By introducing an anoxic phase into an anaerobic-aerobic SBR (AO SBR), significant amounts of denitrifying phosphorus accumulating organisms (DPAOs) which can utilize nitrate as electron acceptor could be accumulated in the reactor (anaerobic-aerobic- anoxic-aerobic SBR, $(AO)_2$ SBR). A direct comparison of phosphorus uptake rate under anaerobic and aerobic conditions showed that the fraction of DPAOs in P-removing sludge were increased from 10% in the AO SBR to 64% in $(AO)_2$ SBR. The $(AO)_2$ SBR showed stable phosphorus and nitrogen removal efficiency: average removal efficiencies of TOC, total nitrogen, and phosphorus were 92%, 88%, and 100%. respectively. Results of the $(AO)_2$ SBR operation and batch tests showed that nitrite (up to 10 mg-N/L) was not detrimental to anoxic phosphorus uptake and could serve as good electron acceptor like nitrate.

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Distribution of Water Masses and Distribution Characteristics of Dissolved Inorganic and Organic Nutrients in the Southern Part of the East Sea of Korea: Focus on the Observed Data in September, 2011 (동해 남부 해역의 수괴 분포와 용존 무기 및 유기 영양염의 분포 특성: 2011년 9월 관측자료를 중심으로)

  • Kwon, Hyeong Kyu;Oh, Seok Jin;Park, Mi Ok;Yang, Han-Soeb
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.17 no.2
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    • pp.90-103
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    • 2014
  • Distribution characteristics of water masses, dissolved inorganic and organic nutrients were investigated in the southern part of the East Sea of Korea in September, 2011. On the basis of the vertical profiles of temperature, salinity and dissolved oxygen, water masses in the study area were divided into 4 major groups, such as WM (water mass)-I, WM-II, WM-III, WM-IV. Their characteristics were similar to Tsushima Surface Water (TSW), Tsushima Middle Water (TMW), North Korea Cold Water (NKCW) and East Sea Proper Water (ESPW), respectively. In the vertical profiles of dissolved nutrients, dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus (DIP) concentrations were highest in the WM-IV, followed by WM-III, WM-II, WM-I. On the contrary, distribution of dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP) were highest in the WM-I, followed by WM-II, WM-III, WM-IV. Although the DIN : DIP ratio in all of the water masses was similar to Redfield ratio(16), the DIN : DIP ratio in mixed layer was about 5.3, indicating that inorganic nitrogen is the limiting factor for the growth of phytoplankton. However, the DON proportion in dissolved total nitrogen (DTN) was about 70% in the mixed layer where inorganic nitrogen is limiting factor. Thus, enriched DON may play an important source of the nutrient for the growth of phytoplankon in the East Sea.