• Title/Summary/Keyword: Nitrogen analysis

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Determination of the Origin in both Dissolved Inorganic Nitrogen and Phytoplankton at the Lake Paldang using Stable Isotope Ratios (δ13C, δ15N, δ15N-NO3 and δ15N-NH4) (질산염 및 식물플랑크톤의 안정동위원소비를 이용한 팔당호 수계내의 질소원 기원 연구)

  • Kim, Min-Seob;Lee, Eun-Jeong;Yoon, Suk-Hee;Lim, Bo-La;Park, Jaeseon;Park, Hyunwoo;Chung, Hyen-Mi;Choi, Jong-Woo
    • Korean Journal of Ecology and Environment
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    • v.50 no.4
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    • pp.452-458
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    • 2017
  • The nitrogen isotope value in both ammonium and nitrate ion were determined at 9 stations during both June and August 2016, in order to understand the origin of DIN at the Han river. ${\delta}^{15}N-NO_3$ and ${\delta}^{15}N-NH_4$ values in 8 stations (CP, SB, MHC, P4, SJ, SBC, P2, SC) were no significant variation. However ${\delta}^{15}N-NO_3$ and ${\delta}^{15}N-NH_4$ values in KK (Kyeongan stream) showed significant different in comparison with 8 stations, with an apparent increase of nitrogen isotope values. These results indicate that antropogenic nitrogen source influence on KK station. Also the ${\delta}^{13}C$ and ${\delta}^{15}N$ isotope ratio of phytoplankton (Diatom and Cyanobacteria) in KK (Kyeongan stream) showed heavier values, compared to other study stations. These results indicate that nitrogen isotope value in phytoplankton effects by different nitrogen source in study sites. These results suggest that the analysis of stable isotope ratios is a simple but useful tool for the identification of dissolved inorganic nitrogen origin in aquatic environments.

A Study on the Thermal Analysis for the Robotic Arm of the Cord Blood Storage Tank (제대혈 용기 내부 로봇 암의 열해석에 관한 연구)

  • Yun, Sang-Kook;You, Sam-Sang
    • Journal of Advanced Marine Engineering and Technology
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    • v.32 no.5
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    • pp.724-729
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    • 2008
  • Umbilical cord blood has been recently considered an attractive potential alternative as a source of stem cell transplantation to curing diseases such as leukemia, cancers, immune disorders. Normally the stored system of the umbilical cord blood specimen is equipped with a computer-controlled robotic arm that enables the samples to locate the identification places in liquid nitrogen tank at regulated temperature as about $-196^{\circ}C$. As the half of robotic arm is in the air and the rest part is submerged in liquid nitrogen, the temperature of robotic arm varies from ambient to liquid nitrogen temperature. In this study the temperature variation of upper part of arm above tank lid was thermally analysed by using the commercial code of Ansys. The result of analysis was that the upper part of robotic arm was seriously frozen due to heat transfer from liquid nitrogen as low as -$120^{\circ}C$. In order to solve the frost problem of robotic arm, small PTFE tube block as resistance material was introduced into the lower part of tank lid instead of the whole stainless steel(SUS) robotic arm. The results showed that the temperature of robotic arm above the lid was higher enough, and this method would be one of the very effective measure to solve the problem.

Nitrogen Mineralization of Cereal Straws and Vetch in Paddy Soil by Test Tube Analysis

  • Cho, Young-Son;Lee, Byong-Zhin;Choe, Zhin-Ryong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.2
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    • pp.102-105
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    • 1999
  • Mineralization of organic N is an important factor in determining the appropriate rate of organic matter application to paddy fields. A kinetic analysis was conducted for nitrogen mineralization of rice, barley, Chinese milk Ovetch (Astragalus sinicus L.; MV) and narrow leaf vetch straw in paddy soil. Nitrogen immobilization occurred rapidly and its rate increased in straw with high C/N ratio. The amount of nitrogen mineralization was rapid in the first year of rice-vetch cropping system. The rate constant (K) depended on the C/N ratio of organic matter. Mineralization of straw increased at high temperature. The amount of available N increment resulted in fast mineralization of straw, especially in rice and barley straw. Chinese milk vetch had the greatest mineralization rate at all temperatures and fertilization levels followed by narrow-leaf vetch. However, rice and barley straws with high C/N ratio immobilized the soil N at the initial incubation duration. Chinese milk vetch or narrow leaf vetch was not effectively mineralized in mixed treatments with rice or barley straw. The mineralization rate of organic matter was mostly affected by the C/N ratio of straw and temperature of incubation. Organic matter with low C/N ratio should be recommended to avoid the immobilization of soil N and the increasing mineralization rate of straw.

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The Formation of Nitride and Enhancement of Mechanical Properties of Al Alloy by Nitrogen Implantation (Al합금에서 질소이온주입에 의한 질화물 형성과 기계적 특성 향상)

  • Jeong, Jae-Pil;Lee, Jae-Sang;Kim, Kye-Ryung;Choi, Byung-Ho
    • Journal of the Korean institute of surface engineering
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    • v.39 no.5
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    • pp.235-239
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    • 2006
  • The aluminum nitride(AlN) layer on Al7075 substrate has been formed through nitrogen ion implantation process. The implantation process was performed under the conditions : 100 keV energy, total ion dose up to $2{\times}10^{18}\;ions/cm^2$. XRD analysis showed that aluminum nitride layers were formed by nitrogen implantation. The formation of Aluminum nitride enhanced surface hardness up to 265HK(0.02 N) from 150HK(0.02 N) for the unimplanted specimen. Micro-Knoop hardness test showed that wear resistance was improved about 2 times for nitrogen implanted specimens above $5\;{\times}\;10^{17}\;ions/cm^2$. The friction coefficient was measured by Ball-on-disc type wear tester and was decreased to 1/3 with increasing total nitrogen ion dose up to $1\;{\times}\;10^{18}ions/cm^2$. The enhancement of mechanical properties was observed to be closely associated with AlN formation. AES analysis showed that the maximum concentration of nitrogen increased as ion dose increased until $5\;{\times}\;10^{17}\;ions/cm^2$.

Effects of Nitrogen on Deformation Behavior of Duplex Stainless Steel (이상 스테인레스강의 변형거동에 미치는 질소의 영향)

  • 이형직;장영원
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2003.05a
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    • pp.49-52
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    • 2003
  • The effects of nitrogen on the deformation behavior of duplex stainless steel have been studied The variation of strength was correlated with the characteristic microstructures. Analysis based on Hall-Petch relation confirmed that nitrogen enhances phase-boundary strengthening effect. The evolution of dislocation structure, slip traces, and misorientation distribution during deformation were also characterized to elucidate the effect of nitrogen on inelastic deformation mechanism.

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Analysis of Soil Total Nitrogen and Inorganic Nitrogen Content for Evaluating Nitrogen Dynamics

  • Lee, Seul-Bi;Sung, Jwa-Kyung;Lee, Ye-Jin;Lim, Jung-Eun;Song, Yo-Sung;Lee, Deog-Bae;Hong, Suk-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.50 no.2
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    • pp.100-105
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    • 2017
  • Various methods for assessing soil total nitrogen (TN) and inorganic N content have been developed to manage nutrient and to understand N cycle in soil. This paper address the technical procedures in arable soil samples to conduct soil sampling, sample preparation, and measuring total N and inorganic N. Among various methods for measuring soil total nitrogen contents, Kjeldahl distillation and Indophenol blue method have widely used due to reliability and economic advances. Also, two methods can analyze more samples at the same time compared with other nitrogen measuring methods. For evaluating inorganic N content, mainly in forms of nitrate-N ($NO_3{^-}-N$) and ammonium-N ($NH_4{^+}-N$), extraction with a single reagent such as 2M KCl has been employed, followed by Kjeldahl distillation or indophenol blue methods.

Effect of Planting Density and Nitrogen Level on Growth and Yield in Heavy Panicle Weight Type of Japonica Rice

  • Kim, Bo-Kyeong;Kim, Hyun-Ho;Ko, Jae-Kwon;Shin, Hyun-Tak
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.2
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    • pp.106-111
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    • 1999
  • To investigate the effects of planting density and nitrogen level on growth and yield potential of newly bred heavy panicle japonica rice with large grain (Iksan 435 and Iksan 438) or many spikelets per panicle(HR14022-21-8-4 and HR14022-21-8-6), four heavy panicle type rices and two many panicle type rices(Dongjinbyeo and Donganbyeo) as the checks were planted under standard planting density (30$\times$15 cm) and dense planting density (15$\times$15 cm) with two nitrogen levels of standard nitrogen level(110 kg h $a^{-1}$) and heavy nitrogen level(165 kg h $a^{-1}$). Effective tiller rate decreased in dense planting or heavy nitrogen, when compared to standard nitrogen and planting, while leaf area index and to dry weight increased in dense planting or heavy nitrogen. Tiller numbers and panicle numbers were more increased by dense planting than heavy nitrogen, whereas spikelet numbers were more increased by heavy nitrogen than dense planting. Ripened grain ratio was slightly lower only in dense planting. 1,000 grain weight in brown rice was not significantly different in dense planting or heavy nitrogen. Milled rice yield was highest in heavy nitrogen with standard planting for heavy panicle type rice, while yield for many panicle type rice was highest in heavy nitrogen with dense planting, suggesting that many panicle type rice possesses higher adapt-ability for dense planting than heavy panicle type rice. Path coefficient analysis revealed that top dry weight, spikelet number and grain weight were the greatest positive contributors to yield, whereas tiller number was negative to yield.d.

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Herd Reproductive Performance Related to Urea Nitrogen Concentration in Bulk Milk (우유내 요소태 질소 농도와 관련된 우군의 번식성)

  • 박수봉;김현섭;이명식;박진기;장원경;이정규
    • Korean Journal of Animal Reproduction
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    • v.21 no.2
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    • pp.191-195
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    • 1997
  • The objective of this study were to compare two methods of analysis of milk urea nitrogen (MUN) and to relate conentration of MUN to pregnancy rate in dairy cows. The Azotest and Sigma method for MUN analysis were compared. The Azotest consistently underestimated the Sigma values for same samples, except for those in rank 3. The CV for the Azotest, within each rank, were less than 15%, demonstrating acceptable repeatability of the Azotest within a rank. Concentrations of MUN higher or lower than MUN in rank 3 and 4 were associated with decreased pregnancy rate. Mun concentrations in rank 5 and 6 were associated with a, pp.oximately a 16 percentage point decrease in pregnancy rate. Mun concentrations in rank 5 and 6 were associated with a, pp.oximately a 16 percentage point decrease in pregnancy rate after AI in dairy cow. This results suggest that Azotest may be benificial to dairy producers to monitor urea nitrogen concentration in their herd in efforts to maintain or improve reproductive efficiency.

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A Study on the Degradation of Mechanical Properties in High Nitrogen Steel Following Heat Treatments and Welding (고질소계 강의 열처리재 및 용접부의 기계적성질 저하에 관한 연구)

  • 권일현;윤재영;정세희
    • Journal of Welding and Joining
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    • v.16 no.3
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    • pp.121-128
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    • 1998
  • The degradation of mechanical properties in the high nitrogen steel HN3 developed for nuclear fusion reactor has been evaluated quantitatively using the small punch(SP) test, X-ray diffraction (XRD) analysis has also been conducted to identify carbides or nitrides precipitated on grain boundaries of the heat treated samples. Mechanical properties of the steel HN3 significantly decreased with increasing heat treatment time and temperature or with decreasing testing temperature. Combination of XRD and metallurgical observation, revealed that the material degradation in the thermally aged steel was caused by precipitation of carbides on the grain boundaries. While the weld metal showed the lowest mechanical properties among various microstructures in GTA weldments. By combining SP test and XRD analysis, cryogenic fracture behaviors and aging degradation for high nitrogen steel could be successfully evaluated in nondestructive manner.

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Analysis of Significant Factor in the Flavor of Traditional Korean Soy Sauce (II) - Analysis of Nitrogen Compounds, Free Amino Acids and Nucleotides and Their Related Compounds - (한국전통간장의 맛과 향에 관여하는 주요 향미인자의 분석(II) - 질소 화합물, 유리아미노산 및 핵산관련물질 분석 -)

  • Park, Hyun-Kyung;Sohn, Kyung-Hee
    • Journal of the Korean Society of Food Culture
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    • v.12 no.1
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    • pp.63-69
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    • 1997
  • This study was carried out in order to analyzed the changes in nitrogen containing taste compounds of three different types of traditional Korean soy sauce with varing ripening period, and to investigates correlation between sensory characteristics and taste compounds contents via contents assay and sensory evaluation of soy sauce samples. Total nitrogen, ammonia type nitrogen and amino type nitrogen contents showed the highest value in Kyupjang. 17 kinds of free amino acid was detected in Chungjangs and 16 kinds of free amino acid was detected in Kyupjang. Nucleotides and their related compounds detected were hypoxanthin, xanthin, IMP, AMP, Inosine, ADP. Free amino acid and nucleotides and their related compounds contents were highest in Kyupjang. Nitrogen related compounds content of high concentration soy sauce ripened over 150 days increased similarly with Kyupjang. In the sensory evaluation of soy sauce taste, Chungjang samples acquired the highest score in the offensive taste test while Kyupjang marked highest score for sweet taste, nutty taste, taste preference. High concentration soy sauce ripend over 150 days yielded the result similar to that of Kyupjang. Sweet taste showed positive correlation with nitrogen compounds. The materials that showed positive correlation with nutty and traditional soy sauce taste and taste preference were nitrogen compound, IMP, AMP. Except for tryptophan, all free amino acid showed positive correlation with nutty and traditional soy sauce taste. Particulary, taste preference correlated to lysine, aspartic acid and glutamic acid.

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