• Asian-Australasian Journal of Animal Sciences
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• v.11 no.6
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• pp.697-701
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• 1998

In order to improve silage quality and utilization of napier grass (Pennisetum purpureum Schum.) by goats, the grass was ensiled with molasses (MOL) and/or defatted rice bran (DRB). Napier grass was harvested at the growing stage in July and cut into 3 cm length. The grass was mixed with 4% MOL and/or 15% DRB, ensiled 15 kg each into plastic bags and stored for 9 months. Dry matter content of the silage ensiled with MOL (MOL-silage) was 13.4%, but increased to 20% with DRB addition. The addition of MOL decreased pH value and ammonia nitrogen content, but increased lactic acid content. MOL-silage contained about 6% spoilage, but addition of DRB decreased spoilage to less than 1%. Goats were fed the silage at a level of 2.25% (DM basis) of their body weight. Goats fed DRB- or MOL/DRB-silages maintained nitrogen retention, but goats fed MOL-silage did not. The rumen fluid of goats fed DRB-silage tended to be higher in acetic acid and lower in propionic acid than those fed the other silages. Ammonia in the rumen fluids, urea nitrogen in the blood and the urinary nitrogen excretion were the lowest in goats fed MOL/DRB-silage. As the result, the ratio of retained nitrogen to nitrogen intake was the highest in goats fed MOL/DRB-silage. In conclusion, addition of DRB to napier grass increased DM of silage and decreased the volume of spoilage. The combination of MOL and DRB can improve the fermentation quality and thus enhance the utilization of the silage by goats, more than the MOL or DRB being as a single treatment.

• Journal of Animal Science and Technology
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• v.62 no.5
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• pp.659-667
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• 2020

This study was conducted to evaluate the effects of different levels of crude protein (CP) and protease on nitrogen (N) utilization, nutrient digestibility, and growth performance in growing pigs. A total of six crossbred ([Landrace × Yorkshire] × Duroc) barrows were individually accepted in 1.2 m × 0.7 m × 0.96 m stainless steel metabolism cages. The pigs (average initial body weight of 27.91 ± 1.84 kg) randomly assigned to six diets with six weeks (6 × 6 Latin square design). The experiment was carried out in an environment with a temperature of 23 ± 1.5℃, a relative humidity of 83 ± 2.3% and a wind speed of 0.25 ± 0.03 m/s. The dietary treatments were arranged in a 2 × 3 factorial design with two levels of CP (15.3% or 17.1%) and three levels of protease (0 ppm, 150 ppm, or 300 ppm). The average daily gain and gain to feed ratio (G:F) tended to increase (p = 0.074) with increasing amounts of protease. The low CP level diet reduced (p < 0.050) urinary and fecal N concentrations, the total N excretion in feces, and increased (p < 0.050) N retention. Different protease levels in the diet did not affect (p > 0.05) at N intake, but supplementation of the diets with 300 ppm protease decreased (p < 0.050) the N concentration in urine and feces and tended to increase (p = 0.061) the percentage of N retention retained of the total N intake. The dietary CP level did not affect (p > 0.050) the apparent total tract digestibility (ATTD) of dry matter, digestible energy (DE), and metabolic energy (ME), but diet supplementation with 300 ppm protease showed higher (p < 0.050) ATTD of DE and ME than in the protease-free diet. Therefore, a low protein diet with protease could improve the utilization of nitrogen, thereby reducing the negative effect of N excretion into the environment while maintaining or increasing growth performance compared to a high protein diet.

• Journal of Animal Science and Technology
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• v.47 no.3
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• pp.379-390
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• 2005

Effects of dietary brown seaweed product levels on performance and metabolism of protein and energy were investigated in broiler chicks that were activated the acute phase response. One day old chicks were fed diets containing either 0.0(basal), 1.0, 2.0 or 4.0 % brown seaweed products for 3 weeks. The acute phase response was activated by injecting i.p. the Salmonella typhimurium lipopolysacharide(LPS) at $2^{nd}$ week of age. The acute phase response lowered nitrogen balance(NB)/ $kg^{0.75}$ (metabolic body size) and highered dietary ME values in birds fed diets containing brown seaweed product. Increase in dietary brown seaweed products levels lowered daily gain, and NB, uric acid nitrogen(UAN) excretion and ME utilization per $kg^{0.75}$ in chicks with the acute phase response. But the dietary brown seaweed product level did not affect the performance of 3 Week old broiler chicks that experienced the acute phase response. And the brown seaweed products 1.0 and 2.0 % diets lessened the feed intake reduction caused by the acute phase response in broiler chicks. The brown seaweed 2.0% diet increased NB / g diet or $kg^{0.75}$ and decreased the excretion of UAN/g diet or $kg^{0.75}$. This result indicated that the brown seaweed was able to interact with the acute phase response and increased protein retention via decreased breakdown of protein in birds fed brown seaweed 2.0% diet.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1273-1273
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• 2001

The efficiency of the luminal fermentation process influences overall efficiency of luminal production, animal health and reproduction. Ruminant production systems have a significant impact on the global environment, as well. Animal wastes contribute to pollution of the environment as ammonia volatilized to the air and nitrate leached to ground water. Microbial protein synthesis in the rumen satisfies a large proportion of the protein requirements of animals. Quantifying the microbial synthesis is possible by using markers for lumen bacteria and protozoa such as nucleic acids, purine bases, some specific amino acids, or by isotopic $^{15}N,^{32}P,\;and\;^{35}S$ labelled feeds. All those methods require cannulated animals, they are time-consuming and some methods are very expensive as well. Many attempts have been made to find an alternative method for indirect measurement of microbial synthesis in intact animals. The present investigations aimed to assess possibilities of NIRS for prediction of purine nitrogen excretion and ruminal microbial nitrogen synthesis by NIR spectra of urine. Urine samples were collected from 12 growing sheep,6 of them male, and 6- female. The sheep were included in feeding experiment. The ration consisted of sorghum silage and protein supplements -70:30 on dry matter basis. The protein supplements were chosen to differ in protein degradability. The urine samples were collected daily in a vessel containing $60m{\ell}$ 10% sulphuric acid to reduce pH below 3 and diluted with tap water to 4 liters. Samples were stored in plastic bottles and frozen at $-20^{\circ}C$ until chemical and NIRS analysis. The urine samples were analyzed for purine derivates - allantoin, uric acid, xantine and hypoxantine content. Microbial nitrogen synthesis in the lumen was calculated according to Chen and Gomes, 1995. Transmittance urine spectra with sample thickness 1mm were obtained by NIR System 6500 spectrophotometer in the spectral range 1100-2500nm. The calibration was performed using ISI software and PLS regression, respectively. The following statistical results of NIRS calibration for prediction of purine derivatives and microbial protein synthesis were obtained.(Table Omitted). The result of estimation of purine nitrogen excretion and microbial protein synthesis by NIR spectra of urine showed accuracy, adequate for rapid evaluation of microbial protein synthesis for a large number of animals and different diets. The results indicate that the advantages of the NIRS technology can be extended into animal physiological studies. The fast and low cost NIRS analyses could be used with no significant loss of accuracy when microbial protein synthesis in the lumen and the microbial protein flow in the duodenum are to be assessed by NIRS.

• Korean Journal of Ichthyology
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• v.10 no.1
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• pp.106-114
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• 1998

In order to estimate energy budget of the rockfish, Sebastes schlegeli juvenile, during the period from parturition to juvenile, of rockfish were reared at constant condition of water temperature($16^{\circ}C$ and $20^{\circ}C$). Energy used by the reared juveniles were calculated from estimates of data on growth, oxygen consumption, nitrogen excretion, feeding and energy content. After parturition, total length of juvenile was 4.56~5.49mm(average 4.97mm, n=20), and the average dally growth rates were 0.50mm at $20^{\circ}C$. The weight-specific oxygen consumption and nitrogen excretion rates decreased with increasing body weight. Temperature significantly affected oxygen consumption and nitrogen execretion rates, with the higher rates at $20^{\circ}C$ than $16^{\circ}C$. During the 25 days from parturition to jllveniles, feeding rates were 65.8cal at $16^{\circ}C$, and 89.2cal at $20^{\circ}C$. The assimilation effeciency estimated by nitrogen content of food and egested feces were 85.21% at $16^{\circ}C$ and $20^{\circ}C$. During the period from parturition to juvenile, energy was used the higher in body growth than in oxygen consumption. The gross growth efficiencies($K_1$)and net growth efficiencies($K_2$) of the rockfish, Sebastes schlegeli juvenile ranged from 43% to 47% and from 50% to 55%, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.4
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• pp.366-371
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• 2011

The sea squirt Halocynthia roretzi is mainly cultured in Tongyeong, Southern coastal area of Korea. This study presents the physiological rates of respiration, excretion, feeding and assimilation efficiency of the sea squirt Halocynthia roretzi to analyze the SFG(scope for growth) and net growth efficiency, determined during 2007. Oxygen consumption and nitrogen excretion rates increased with a rise in temperature during the summer period whereas feeding rates decreased. The O:N ratio was high during winter(October to February). Assimilation efficiency showed an annual average of 75.4% during the experimental period, except during a period of elevated temperature in July to September(average $25^{\circ}C$). Net growth efficiency($K_2$) was 8.7 to 64.2% except for May to September, when temperature increased at the aquaculture farm. SFG was negative from May to September, reflecting high temperatures and low feeding rates during this period; its highest positive values occurred during winter.

• Journal of Pharmaceutical Investigation
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• v.15 no.2
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• pp.73-82
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• 1985

Each parching Anemarrhenae Rhizoma with 25% ethanol and normal saline has been used for anti-inflammatory, expectorant, antipyretic, sedative and diuretic, and so on. In order to investigate the differences of referential efficacy about each fractionated part of the aqueous extracts, pharmacological studies were carried out. The results of studies were summerized as follows : 1. By the administration of 25% ethanol treated preparation (F-I-1), the increase in urinary volume and $Na^+$ extraction was significantly recognized. 2. The excretion of electrolyte $Na^+$ by saline solution treated preparation (F-II-1) was significantly recognised in normal rats. 3. The increase of urinary volume and urinary $Na^+$ and $Cl^-$ excretion by F-I-1 and n-butanol insoluble parts of F-I-1 (F-I-3) was significantly recognized in $HgCl_2-induced$ acute renal failure of rats. 4. The increase of plasma $Na^+$ by F-I-1 and urea nitrogen by F-I-1 and F-I-3 in acute renal failure of rats was significantly recognized. 5. The increase of urinary volume by F-I-1 and F-I-3 was recognised in mice.

• Journal of Life Science
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• v.11 no.3
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• pp.203-210
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• 2001

To produce the instant marsh calm(Corbicula elatior) soup with abundant taste and flavor. optimum conditions of heating temperature and time for extraction were investigated. Excretion rate in a pre-treatment for excretion of estuary was 82% at 15% salt concentration for 6 hr and 95% at pH 7.5 for 10 hr. The contents of glycogen and amino-nitrogen were the most high levels in the treatments extracted at 9$0^{\circ}C$ for 3 hr, at 10$0^{\circ}C$ for 1.5 hr and at 11$0^{\circ}C$for 1 hr. The contents of total free amino acids in the extracts treated at 9$0^{\circ}C$, 10$0^{\circ}C$ and 11$0^{\circ}C$ were 367.05mg/100g, 472.23 mg/100g and 463.54 mg/100g, respectively and optimal temperature was above 10$0^{\circ}C$. The mineral contents was the most high level for the extract treated at 10$0^{\circ}C$ for 1.5hr. The results of sensory evaluation showed that tastes for the marsh calm extracts had no significant discrimination, while external appearances and flavors for the extract done at 10$0^{\circ}C$ for 1.5 hr were excellent. The extracts at 10$0^{\circ}C$ showed a little dark color and those at 9$0^{\circ}C$ smell a little fish flavor.

• Korean Journal of Organic Agriculture
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• v.9 no.1
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• pp.51-60
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• 2001

An experiment was carried out to evaluate the effects of adding phytase on nutrient digestibility in growing pigs. Three barrow pigs averaging 28.5$\pm$3.1kg of body weight were allotted to three treatments by 3$\times$3 latin square. Treatments included 1) com-soybean meal based-control diet and 2) and 3) control diet with phytase 500 and 1,000 unit. There were no effects of treatments on dry matter and nitrogen digestibility (P〉0.05). Ash, Ca and P digestibility in pigs fed diet with phytase were greater than those in pigs fed control diet(P〈0.05). An increased phosphorus intake was observed from pigs fed diet with treatments compared to that from pigs fed control diet. Pigs fed diet with adding phytase had improved P absorption compared to pigs fed control diet(P〈0.05). There was no significantly effect on fecal and urine excretion(P〈0.05). Ca intake was increased for pigs fed adding phytase compared to pigs fed control diet. No evident effect was observed on Ca fecal excretion among dietary treatments, however, urine excretion of pigs fed diet with treatments was decreased compared to pigs fed control diet. Cystine, isoleucine, tyrosine and phenylalanine digestibility of amino acid were greater for pigs fed diet with treatments than those for pigs fed control diet(P〈0.05). Asparagine, threonine, serine, methionine and leucine digestibility were improved for pigs fed with phytase 500 unit compared to pigs fed control diet. Lysine, histidine, arginine and other animo acids digestibility were higher for pigs fed diet with phytase than control diet, but there were no significant effects by the treatments(P〉0.05). In conclusion, the results of the experiments suggest that phytase supplementation in the diets could be a very beneficial strategy in environmental aspects.

• Journal of Nutrition and Health
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• v.18 no.3
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• pp.194-200
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• 1985

The purpose of this study was to investigate sodium intake and protein metabolism for pregnant women in Korea. The relationship between salt consumption and hypertension, electrolyte changes in pregnancy, and the tendency of weight gain of pregnant women were also examined. The dietary survey and twelve or twenty-four hour's urine collection was performed in this study. The subjects were pregnant women who came to gynecology clinic for prenatal care from July to August in 1984. The results were as follows: 1) Intakes of calorie and iron of Korean pregnant women were 2,036 Kcal and 16mg per day per person. The calorie composition consists of 65% carbohydrate, 16% protein, and 19% lipids. The average level of all nutrients was about 87% of Korean Recommended Dietary Allowances. 2) The sodium intakes in pregnant women estimated by dietary survey was 318.6 mEq Na (7,966 mg Na), which is equivalent to 20.2 g NaCl. 3) Daily sodium excretion was 112.5 mEq per liter, that is equal to 147.4 mEq. Potassium excretion was 24.95 mEq per liter and creatinine excretion was 594.0 mg per day. 4) There were statistically significant correlations between sodium intake and the excretions of sodium, potassium and creatinine. The blood pressure was positively correlated with sodium intakes. As sodium and potassium ratio was increased, systolic blood pressure and diastolic blood pressure were also significantly increased 5) Urinary nitrogen and creatinine ratio known to be reliable index for nutritional status of protein was 5.5 : 1. These values were comparable to the values in American pregnant women. 6) The weight gain during the pregnancy was examined. There was little weight gain during the first trimester, because of nausea, vomit and decrease of appetite. The weight gain during the second trimester was 4.9Kg, and 11.1Kg for third trimester.