• Journal of Biosystems Engineering
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• 제38권4호
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• pp.335-340
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• 2013

Purpose: This study was performed to develop a lateral flow strip sensor for the detection of pathogenic Escherichia coli O157:H7 in various samples. Also, feasibility of using an image analysis method to improve the interpretation of the strip sensor was evaluated. Methods: The lateral flow strip sensor has been fabricated based on nitrocellulose lateral-flow membrane. Colloidal gold and E. coli O157:H7 antibodies were used as a tag and a receptor, respectively. Manually spotted E. coli O157:H7 antibody and anti-mouse antibody on nitrocellulose membrane were used as test and control dots, respectively. Feasibility of the lateral flow strip sensor to detect E. coli O157:H7 were evaluated with serially diluted E. coli O157:H7 cells in PBS or food samples. Test results of the lateral flow strip sensor were measured with an image analysis method. Results: The intensity of the test dot started to increase with higher concentration of the cells were introduced. The sensitivities of the sensor were both $10^4$ CFU/mL Escherichia coli O157:H7 spiked in PBS and in chicken meat extract, respectively. Conclusions: The lateral flow strip sensor and image analysis method could detect E. coli O157:H7 in 20 min, which is significantly quicker than conventional plate counting method.

• Korean Chemical Engineering Research
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• 제48권6호
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• pp.695-699
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• 2010

추진제 KM10은 니트로셀룰로오스를 주원료로 제조된 단기추진제로서 장기저장 시자연분해현상을 일으키는 것으로 알려져 있다. 따라서 본 연구에서는 고온가속노화시험과 저장분석시험을 이용하여 추진제의 저장수명을 추정하였다. 고온가속 노화시험을 이용한 저장수명추정은 Arrhenius 식과 Berthelot 식을 사용하였으며, 저장분석시험을 이용한 저장수명 예측은 1차 회귀직선식을 이용하였다. 본 연구 결과에 따르면 고온가속 노화시험의 Arrhenius 식과 Berthelot식을 이용하여 추정한 추진제 KM10의 저장수명은 43.72년, 16.53년으로 큰 차이를 보였으며, 저장분석시험으로 이용한 저장수명은 42.94년으로 나타났다. 이것을 E. R. Bixon의 연구결과와 비교할 때 Arrhenius 식을 이용하여 추정한 값이 타당한 것으로 판단되었다.

• BMB Reports
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• 제28권2호
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• pp.143-148
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• 1995

In E. coli, chromosomal DNA associated with proteins is condensed into an organized structure known as nucleoid. Using a nitrocellulose filter binding assay to identify proteins forming nucleoid, a 21 kDa protein was purified from E. coli. The molecular weight of the purified protein was 21 kDa on SDS-polyactylamide gel electrophoresis and 24 kDa on gel permeation chromatography. A molecular weight of 21 kDa on SDS-polyacrylamide gel electrophoresis is unique among known proteins which are believed to be involved in the formation of nucleoid in E. coli. The 21 kDa protein nonspecifically binds to both double-stranded and single-stranded DNA. Sedimentation in a sucrose gradient revealed that the protein induced significant condensation of both supercoiled plasmid DNA and linear bacteriophage $\lambda$ DNA On the basis of quantitative Western-blot analysis, approximately 40,000 molecules of the protein were estimated to exist in an E. coli. The biochemical properties and cellular abundance of the 21 kDa protein suggest that this protein participates in the formation of nucleoid in E. coli.

• Natural Product Sciences
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• 제5권1호
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• pp.39-47
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• 1999

The flowers of Alcea rosea L., Malvaceae, sold in the Indian market under the trade name 'Gulkhairo', are well known for their expectorant, cooling and diuretic properties and used in many indigenous cough mixtures in India. The present paper deals with the detailed pharmacognosy of the floral parts including morphological, anatomical, phytochemical and fluorescence characters. Some of the diagnostic features of the drug are : pedicel characterized by multicellular appendages, stellate hairs, rosette crystals of Ca-oxalate, starch sheath and large sized mucilage canals; sepals having distinctive multicellular appendages arranged in a semilunar fashion present adaxially at their base; monadelphous stamens, pollen grains pentaporate provided with dimorphic spines; placentation axile, ovules campylotropous; dark green fluorescence of the powder with nitrocellulose in amyl acetate and yellow fluorescence of trichomes under Fluorescence microscope.

• Journal of the Korean Wood Science and Technology
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• 제17권1호
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• pp.12-21
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• 1989

This experiment was executed to investigate the characteristics of gloss and color difference in coated films by N.C. Lacquer, Aminoalkyd, Polyester. and Polyurethan coating after chemical (distilled water, ethyl alcohol. acetic acid, and sodium hydroxide) and heating ($120^{\circ}C$) treatments, cold-check test, and U.V. radiation. The results obtained were summarized as follows 1. The gloss decreasing rate by water resistance test was the least among chemicals treated on coated films. 2. The color difference of coated films chemical treatments highly and similarly increased, except the alkali treatment showing a little increase. 3. In the color difference by U.V. radiation, the polyester coated film showed generally large difference compared with the other coated films.

• 한국군사과학기술학회지
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• 제9권1호
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• pp.13-23
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• 2006

A propellant mainly consisting of nitric ester including nitrocellulose and nitroglycerine is characteristic of being decomposed naturally. And this phenomenon is known as being affected mostly by its storing temperature and humidity. In this research, the effect of storing temperature and humidity on self life has been studied by measuring the contained quantity of residual stabilizer of propellant KM30Al, which are parts of 155MM propelling charge K676 and K677; the method for the measurement is acceleration aging test, and decomposition reaction equation, Eyring Equation and Berthlot Equation were used for the calculation. As result of this study, it was found that the storing temperature influenced seven times as large as the storing humidity upon the self life of the propellant KM30Al, Furthermore, especially in the high temperature region, the storing temperature had a dominant effect on the self life.

• 대한임상검사과학회지
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• 제43권3호
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• pp.120-123
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• 2011

A total of 30 water samples were collected from 30 different public baths in Seoul, Korea. Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to increase background rates of disease. Pathogens in water was filtered by nitrocellulose membrane with $0.45{\mu}m$ pore size. The membrane filters were analyzed by both cultivation and polymerase chain reaction (PCR) amplification of partial 16S rRNA gene. Various microorganisms including 4 Escherichia coli/Shigella spp. 1 Salmonella spp. 3 Pseudomonas aeruginosa and 2 Mycobacterium spp. were identified by reverse blot hybridization assay (REBA). PCR-REBA was able to identify many bacterial genera in one assay. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.

• KSBB Journal
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• 제5권4호
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• pp.315-321
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• 1990

Rabbit anti-bovine heart PDH antiserum was raised against El(a, b) isolated from PDC, and then applied to detect Ela and Elb. Appropriate amounis of El were fractionated by SDS-PAGE and electrophoretically transferred to nitrocellulose membrane. The Ela and Elb on the membrane were incubated with anti-El antiserum and identified by GAR-HRP system. It has been found that the immunodetection sensitivity of Ela and Elb were directly proportional to the amount of antigen and transfer time. The lengthy transfer times increased the immunodetection sensitivity of Ela and Elb. The maximal detection sensitivity of Western blotting of Ela and Elb was achieved at 3.5 V/cm for 16-hour transfer under these experimental conditions.

• 미생물학회지
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• 제26권3호
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• pp.149-154
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• 1988

Japanese Encephalitis Virus(JEV) can be detected conveniently by the use of biotinylated cDNA probe. To prepare biotinylated probe aminoallyl dUTP was first synthesized chemically to reverse transcribe the virial RNA. The allylamine-labeled cDNA was then converted to the biotin-cDNA by the reaction with an activated biotin ester, NHS-ACA-biotin. The JEV genomic RNA was hybridized to the biotinylated cDNA probe on nitrocellulose filter and visualized colorimetrically by streptavidin complexes with alkaline phosphatase polymer. Sensitivity of the detection system was determined by estimating the amount of the JEV genomic RNA through comparison with signals generated from the biotinylated and $^{32/P}$ -labeled probes. It was found that the biotin probe was as sensitive as $^{32/P}$ -cDNA probe which can detect 50pgs of the target RNA.

• 한국군사과학기술학회지
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• 제8권1호
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• pp.14-24
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• 2005

A propellant mainly consisting of nitric ester including nitrocellulose, nitroglycerine and nitroguanidine is characteristic of being decomposed naturally. And this phenomenon is known as being affected mostly by its storing temperature. In this research, the effect of storing temperature on self life has been studied by measuring the contained quantity of residual stabilizer of propellant KM30A1, ignition powder and combustible cartridge case, which are parts of 155MM propelling charge K676 and K677; the method for the measurement is acceleration aging test, and decomposition reaction equation and Berthlot Equation were used for the calculation. The result of this research shows that propellant KM30A1, ignition powder, combustible cartridge case in order of decreasing self life, and the self life decreases to 1/3 as the temperature increases by $10^{\circ}C$.