• Korean Journal of Soil Science and Fertilizer
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• v.35 no.2
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• pp.69-76
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• 2002

To study the behavior of $NH_4{^+}$ of CMS (condensed molasses solubles) in soil, a laboratory incubation experiment was conducted during a period of up to 21 days at $25^{\circ}C$. The $NH_4{^+}$ of CMS was labeled with $^{15}N$ and was applied to water-unsturated and water-saturated conditions. Soil pH was gradually decreased from 6.1 to 5.4 under unsaturated condition. However, soil pH was increased to 6.5 within 2 days under saturated condition and then was constant. The concentration of ammonium was decreased 3 times faster under unsaturated condition than under saturated condition. The concentration of nitrate was increased from 17.4 to $155.4mg\;kg^{-1}$ under unsaturated condition. But concentration of nitrate was kept with low(below $8.0mg\;kg^{-1}$) under saturated condition. During the incubation, 52.4% of $^{15}NH_4{^+}$ applied was existed in the form of $NO_3{^-}$ by nitrification under unsaturated condition. Most of applied nitrogen was immobilized within 4 days of incubation. On 21 days of the incubation the percentage of immobilized nitrogen derived from $^15NH_4{^+}$(NDFA) was 19.6% under unsaturated condition and 17.0% under saturated condition. The percentage of unaccounted N, which was lost by denitrification, was 28.4% under unsturated condition and 67.6% under saturated condition.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.1
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• pp.42-47
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• 2006

The biological nutrient treatment is formed with repetition and rearrangement of anaerobic, anoxic and oxic tank. In this case, VFAs is generated in the anaerobic tank and the anoxic tank. The VFAs is an important factor for removal of nitrogen and phosphate and SVI. So, in this study I investigated to find a relationship among the generation rate of the VFAs according to the change of F/M ratio and the characteristic which can eliminate organic matter and nitrogen according to the change of residual concentration of the VFAs and the efficiency of the process and also SVI in wastewater treatment. $A^2/O$ process was used for wastewater treatment. F/M ratio was under the control of the change of MLSS concentration. When the F/M ratio was changed from 0.16 to 0.08 kg-BOD/kg-MLSS/day, the VFAs's production volume increased based on the reduction of F/M ratio in batch reaction. And the residual concentration of the VFAs decreased at first and then increased later. SVI and SS were high when F/M ratio was $0.16kg/kg{\cdot}d$ and showed stable status when F/M ratio decreased $0.11{\sim}0.13kg/kg{\cdot}d$. However, SVI and SS continuously increased with decrease of F/M ratio and were high at $0.08kg/kg{\cdot}d$. In the result of comparison between residual concentration of the VFAs and denitrification rate in anoxic tank, the less residual volume of the VFAs was in anoxic tank, the higher denitrification ratio became. The optimal residual-concentration of the VFAs considering SVI and removal efficiency of nitrogenwas $1.4{\sim}2.2mg/L$. At that time F/M ratio was $0.11{\sim}0.13$ kg-BOD/kg-MLSS/day.

• Korean Journal of Environmental Agriculture
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• v.22 no.4
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• pp.278-283
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• 2003

This study was carried out to examine the nitrogen removal rate of a subsurface-flow treatment wetland system which was constructed on floodplain of the Kwangju River from May to June 2001. Its dimensions were 29m in length, 9m in width and 0.65m in depth. A bottom layer of 45cm in depth was filled with crushed granite with about $15{\sim}30\;mm$ in diameter and a middle layer of 10cm in depth had pea pebbles with about 10 mm in diameter. An upper layer of 5 cm in depth contained course sand. Reeds (Phragmites australis) were transplanted on the surface of the system. They were dug out of natural wetlands and stems were cut at about 40 cm height from their bottom ends. Water of the Kwangju River flowed into it via a pipe by gravity flow and its effluent was funneled back into the river. The height of reed stems was 44.2 cm in July 2001 and 75.3cm in September 2001. The number of stems was increased from $80\;stems/m^2$ in July 2001 to $136\;stems/m^2$ in September 2001. Volume and water quality of inflow and outflow were analyzed from July 2001 through December 2001. Inflow and outflow averaged 40.0 and $39.2\;m^3/day$, respectively. Hydraulic detention time was about 1.5 days. Average nitrogen uptake by reeds was $69.31\;N\;mg/m^2/day$. Removal rate of $NO_3-N$, $NH_3-N$, T-N averaged 195.58, 53.65, and $628.44\;mg/m^2/day$, respectively. Changes of $NO_3-N$ and $NH_3-N$ abatement rates were closely related to those of wetland temperatures. The lower removal rate of nitrogen species compared with that of subsurface-flow wetlands operating in North America could be attributed to the initial stage of the system and inclusion of two cold months into the six-month monitoring period. Increase of standing density of reeds within a few years will develop both root zones suitable for the nitrification of ammonia and surface layer substrates beneficial to the denitrification of nitrates into nitrogen gases, which may lead to increment in the nitrogen retention rate.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.1
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• pp.111-122
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• 2009

Abatements of TN and ${NO_3}^-$-N in a horizontal subsurface-flow wetland with litter layer on its surface were compared with those without one. The wetland was constructed in 2001 on floodplain of the Gwangju Stream which flows through Gwangju City in Korea. Its dimensions were 29m in length, 9m in width and 0.65m in depth. A bottom layer of 45cm was filled with crushed granites (15~40mm in diameter) and a middle layer of 10cm had pea pebbles. An upper layer of 5cm contained coarse sands. Reeds (Phragmites australis) growing in natural wetlands were transplanted on its surface. Water of the stream was channelled into the wetland by gravity flow and its effluent was discharged back into the stream. Average Litter layer of 12.2cm was formed on its surface in 2007. The layer and above-ground parts of reeds were eliminated in April 2008. Volumes and water quality of influent and effluent of the wetland were analyzed from May to November in 2007 and 2008, respectively. Inflow into the wetland both in 2007 and 2008 averaged approximately 40$m^3$/day and hydraulic residence time both in 2007 and 2008 was about 1.5days. Influent TN concentration in 2007 and 2008 averaged 3.96 and 3.89mg/L, respectively and average influent ${NO_3}^-$-N concentration in 2007 and 2008 was 2.11 and 2.05mg/L, respectively. With a 0.05 significance level, influent concentrations of TN and ${NO_3}^-$-N, temperatures and pH of effluent, and heights and stem numbers of reeds showed no difference between the wetland with litter layer and without one. TN retention in the wetland with litter layer and without one averaged 64,76 and 54.69%, respectively and ${NO_3}^-$-N removal averaged 60.83 and 50.61%, respectively. Both TN and ${NO_3}^-$-N abatement rates in the wetland with litter layer were significantly high (TN abatement: p<0,001, ${NO_3}^-$-N abatement: p=0.001) when compared with those without one. The subsurface-flow wetland having litter layer on its surface was more efficient for TN and ${NO_3}^-$-N removal.

• Environmental Engineering Research
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• v.10 no.5
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• pp.213-226
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• 2005

To treat wastewater efficiently by a one-step process of nitrogen removal, a new bacterial strain producing $N_2$ gas from ${NH_4}^+$ under an aerobic condition was isolated and identified. The cell was motile and a Gram-negative rod, and usually occurred in pairs. By 16S-rDNA analysis, the isolated strain was identified as Enterobacter asburiae with 96% similarity. The isolate showed that the capacity of $N_2$ production under an oxic condition was approximately three times higher than that under an anoxic condition. Thus, the consumption of ${NH_4}^+$ by the isolate was significantly different in the metabolism of $N_2$ production under the two different environmental conditions. The optimal conditions of the immobilized isolate for $N_2$ production were found to be pH 7.0, $30^{\circ}C$ and C/N ratio 5, respectively. Under all the optimum reaction conditions, $N_2$ production by the immobilized isolate resulted in reduction of ORP with both the consumption of DO and the drop of pH. The removal efficiencies of $COD_{Cr}$, and TN were 56.1 and 60.9%, respectively. The removal rates of $COD_{Cr}$, and TN were the highest for the first 2.5 hrs with the removal $COD_{Cr}/TN$ ratios of 32.1, and afterwards the rates decreased as reaction proceeded. For application of the immobilized isolate to a practical process of ammonium removal, a continuous operation was executed with a synthetic medium of a low C/N ratio. The continuous bioreactor system exhibited a satisfactory performance at 12.1 hrs of HRT, in which the effluent concentrations of ${NH_4}^+$-N was measured to be 15.4 mg/L with its removal efficiency of 56.0%. The maximum removal rate of ${NH_4}^+$-N reached 1.6 mg ${NH_4}^+$-N/L/hr at 12.1 hrs of HRT(with N loading rate of $0.08\;Kg-N/m^3$-carrier/d). As a result, the application of the immobilized isolate appears a viable alternative to the nitrification-denitrification processes.

• Journal of Microbiology
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• v.45 no.2
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• pp.113-121
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• 2007

The bacterial diversity inherent to the biofilm community structure of a modified rotating biological contactor wastewater treatment process, referred to as the Rotating Activated Bacillus Contactor (RABC) process, was characterized in this study, via both culture-dependent and culture-independent methods. On the basis of culture-dependent methods, Bacillus sp. were found to exist in large numbers on the biofilm (6.5% of the heterotrophic bacteria) and the microbial composition of the biofilms was quite simple. Only three phyla were identified-namely, the Proteobacteria, the Actinobacteria (High G+C Gram-positive bacteria), and the Firmicutes (Low G+C Gram-positive bacteria). The culture-independent partial 16S rDNA sequence analysis revealed a considerably more diverse microbial composition within the biofilms. A total of eight phyla were recovered in this case, three of which were major groups: the Firmicutes (43.9%), the Proteobacteria (28.6%), and the Bacteroidetes (17.6%). The remaining five phyla were minor groups: the Planctomycetes (4.4%), the Chlorobi (2.2%), the Actinobacteria (1.1%), the Nitrospirae (1.1%), and the Verrucomicrobia (1.1%). The two most abundant genera detected were the endospore-forming bacteria (31.8%), Clostridium and Bacillus, both of which are members of the Firmicutes phylum. This finding indicates that these endospore-forming bacteria successfully colonized and dominated the RABC process biofilms. Many of the colonies or clones recovered from the biofilms evidenced significantly high homology in the 16S rDNA sequences of bacteria stored in databases associated with advanced wastewater treatment capabilities, including nitrification and denitrification, phosphorus accumulation, the removal of volatile odors, and the removal of chlorohydrocarbons or heavy metals. The microbial community structures observed in the biofilms were found to correlate nicely with the enhanced performance of advanced wastewater treatment protocols.

• Journal of Applied Biological Chemistry
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• v.43 no.2
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• pp.86-93
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• 2000

An experiment was conducted to obtain the quantitative data on the transformation and loss of applied urea-N in waterlogged soil columns. The soil columns were pre-incubated for 35 days to develop oxidized and reduced soil conditions prior to urea application. After urea application at the rate of $150kg\;N\;ha^{-1}$(29.5 mg N), the amounts of nitrogen which were volatilized, leached, and remained in soil column were measured during 38 days of incubation period. On 2 and 4 days of incubation, 54.1%(15.9 mg N) and 98.4%(29.0mg N) of the applied urea was hydrolyzed, respectively. Most of the applied urea was completely hydrolyzed within 6 days. After urea application, the rates of ammonia volatilization were increased with the floodwater pH when the floodwater pH were higher than 7.0. The maximum rate of ammonia volatilization was $0.3mg\;d^{-1}$ when pH of the floodwater showed maximum value of 7.6. The total amount of volatilized nitrogen was 6.1% (1.8mg N) of the applied urea-N. A 63.2 % (18.6mg N) of the applied urea was remained in soil as $NH_4{^+}-N$ and 28.0% (8.2mg N) of the applied urea was leached as $NH_4{^+}-N$ at the end of the incubation. Amount of $NO_3{^-}-N$ in soil was smaller than 2.0 mg throughout the incubation period. The total amount of $NO_3{^-}-N$ leached was very small, which value was 1.8 mg. It suggested that nitrification process was not significant in waterlogged soil column of this study due to high infiltration rate of urea solution applied to the soil column. Therefore only small amount of $NO_3{^-}-N$ was lost by denitrification and leaching process.

• Journal of Wetlands Research
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• v.23 no.1
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• pp.60-66
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• 2021

As the concentration of nitrogen in the sewage flowing into the sewage treatment plant increases due to urbanization and industrialization, the degree of adverse effects such as eutrophication and toxicity to the aquatic ecosystem is also increasing. In order to treat sewage containing high concentration of nitrogen, various studies on the biological nitrogen removal process are being conducted. Existing biological nitrogen removal processes require significant costs for supplying oxygen and supplementing external carbon sources. In this respect, as a high-level nitrogen removal process with economic improvement is required, an anaerobic ammonium oxidation process (ANAMMOX), which is more efficient and economical than the existing nitrification and denitrification processes, has been proposed. The purpose of this study is to confirm the stability of the ANAMMOX process in the water treatment process and to derive the ratio of ammonia nitrogen (NH4+) to nitrite nitrogen (NO2-) for the implementation of the mainstream ANAMMOX process. A laboratory-scale Mainstream ANAMMOX reactor was operated by applying the ratio calculated based on the substrate ratio suggested in the previous study. In the initial range, the removal efficiency of NH4+ was 58~86%, and the average removal efficiency was 70%. In the advanced range, the removal efficiency of NH4+ was 94~99%, and the average removal efficiency was 95%. As a result of the study, as the NH4+/NO2- ratio increased, the stability of the mainstream ANAMMOX process was secured, and it was confirmed that the NH4+ removal efficiency and the total nitrogen (TN) removal efficiency increased. As a result, the results of this study are expected to be used as basic data in the application of the ANAMMOX process in the mainstream.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.955-960
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• 2012

In most agricultural soils, ammonium ($NH_4{^+}$) from fertilizer is quickly converted to nitrate ($NO_3{^-}$) by the process of nitrification which is crucial to the efficiency of N fertilizers and their impact on the environment. The salinity significantly affects efficiency of N fertilizer in reclaimed tidal soil, and the soil pH may influence the conversion rate of ammonium to nitrate and ultimately affect nitrogen losses from the soil profile. Several results suggest that pH has important effects on recovery of fall-applied N in the spring if field conditions are favorable for leaching and denitrification except that effects of soil pH are not serious under unfavorable conditions for N loss by these mechanisms. Soil pH, therefore, deserves attention as an important factor in the newly reclaimed tidal soils with applying N. However, fate of N studies in a newly reclaimed tidal soils have been rarely studied, especially under the conditions of saline-sodic and high pH. Therefore, understanding the fate of nitrogen species transformed from urea treated into the reclaimed tidal soil is important for nutrient management and environmental quality. In this article, we reviewed yields of rice and fate of nitrogen with respect to the properties of reclaimed tidal soils.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.7
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• pp.485-491
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• 2011

This study was performed to investigate the characteristics of nutrient removal of municipal wastewater in membrane bioreactor system. Membrane bioreactor consists of four reactors such as two intermittently anaerobic tanks, the oxic tank and the sludge solubilizaion tank with an internal recycle. The hydraulic retention time (HRT) and flux were 6.5 hours and $20.4L/m^2{\cdot}hr$ (LMH), respectively. The removal efficiency of $COD_{Cr}$, SS, TN and TP were 94.0%, 99.3%, 99.9%, 69.9%, and 66.9%, respectively. The estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR), specific phosphorus release rate (SPRR) and specific phosphorus uptake rate (SPUR) were 0.34 kgVSS/kgBOD d, $0.067mgNO_3-N/mgVSS{\cdot}d$, $0.028mgNH_4-N/mgVSS{\cdot}d$, 16.0 mgP/gVSS d and 2.1 mgP/gVSS d, respectively. The contents of nitrogen and phosphorus of biomass were 8.9% and 3.5% on an average.