• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.6
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• pp.721-730
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• 2017

This study determined the optimal mixing ratio of functional materials to enhance the functionality of batter for fish cutlets. An optimal mixing ratio of the hiziki Hizikia fusiformis, fig fruit Ficus carica L. and pear Pyrus pyrifolia Niitaka extracts (HFP extract) of 3:1:1 was found. The physicochemical properties of the mixture were as follows: total phelonic contents of $1187.29{\pm}41.55mg/L$, 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity of $61.50{\pm}2.33%$, ascorbic acid content of $23.68{\pm}0.02g/100g$, ${\beta}-carotene$ content of $51{\pm}3.92{\mu}g/100g$, and sugar content of $12.7{\pm}0.1Brix^{\circ}$. A mixture of the extract, Korean herbal powder, and wheat flour was prepared as the fish cutlet batter. The fish cutlet prepared with the batter had a hardness of $166-202g/cm^2$ and Hunter's color scale values L of $31.14{\pm}2.1$ to $34.62{\pm}1.3$, a of $-4.89{\pm}0.21$ to $-2.26{\pm}0.19$ and b of $-20.65{\pm}2.65$ to $28.93{\pm}3.14$. The FRAP (ferric reducing antioxidant power) and VBN (volatile basic nitrogen) of the fish cutlets were measured to evaluate functionality and determine the optimal mixing ratio for cold storage at $-12^{\circ}C$, $-18^{\circ}C$, and $-24^{\circ}C$. The optimum mixture ratio of extracts /Korean herbal powder/ wheat flour was 1:6:3.

• Korean Journal of Organic Agriculture
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• v.17 no.4
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• pp.567-575
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• 2009

Organic pear (Pyrus pyriforia) fruits have remarkably increased in Korea since year 2000 due to increase consumer's demand for the fruit, and the farmers are keep trying to change their cultivation system that is replaced from conventional farming to organic-base farming for the fruit. However, there is little information for the soil properties, tree nutrition, and tree growth at organic orchards in Korea. Therefore, this study was established to investigate for the chemical properties of soils and leaf and bud characteristics of a conventional and organic pear trees in experimental orchards for two years. Commercial (Sanwool, YoungHwa Co., Korea) compost was applied to the orchard soils with conventional farming systems for two years for pear trees (10 to 15 year old trees) in KyungSan, Korea. The compost application increased pH, organic matter content, cation exchange capacity (CEC), and, microbial biomass in the soil. The compost treatment also increased specific leaf weight and foliar $P_2O_5$ concentration as well as leaf and floral bud sizes.

• Korean Journal of Environmental Agriculture
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• v.26 no.2
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• pp.171-178
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• 2007

Diffusion and accumulation of ethoprophos in fruit such as apple and two types of pears were examined by dipping them into the solution of ethoprophos. The effective diffusivities of ethoprophos at the skin and flesh of apple and pear were determined by simulation their experimental accumulation curves with calculated ones from the model assuming consecutive diffusion of ethoprophos from skin to flesh. Its effective diffusivity at the flesh with higher content of water were higher, $\sim10^{-10}\;m^2/s$, regardless of the types of fruits, while that at the skin was small, $\sim10^{-12}\;m^2/s$ and increased with the order of 'Niitaka' pear < 'Whangkeumbae' pear < 'Fuji' apple. The variation in the concentration of ethoprophos in its solution did not induce any change in affecting its effective diffusivity at the flesh of 'Whangkeumbae' pear, but the increase in the concentration caused the increase in its effective diffusivity at the skin. The penetration rate of ethoprophos at the skin was an important factor in determining its accumulation rate in fruit.

• Korean Journal of Plant Resources
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• v.20 no.6
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• pp.563-569
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• 2007

RAPD analysis showed that all the OTUs of 'Sandolbae' were the same species because amplified band patterns of all samples generated by each of 5 random primers were identical. Even though there were different environmental conditions, all the "Chuiangne" trees from three different places were the same species, and also all the "Cheongshilli" trees were the same species too. No genetic variations were detected between native Korean pears grown in the habitats and in the research field. Because 212 polymorphic bands were generated by 9 primers selected through primer screening, they were possible to analyze genetic relationship among naturally growing three native Korean pears and nine cultivars of Pyrus pyrifolia and P. communis. Based on the RAPD analysis, three main groups were formed. The first group represented the Six P. pyrifoia cultivars, the second group was the three native Korean pears, and the last group was the three P. communis cultivars. Genetic distance between 'Wonwhang' and 'Chojuro' was closer than other cultivars in group 1 since dissimilarity index value between these two cultivars was 50.82. However, genetic distance between 'Niitaka' and 'Chojuro' was the most distant compared to the others in group 1. In group 2, 'Sandlobae' was genetically closer to 'Chuiangne' than 'Cheongshilli' because dissimilarity index value between 'Sandlobae' and 'Chuiangne' was smaller, 50.82, than the value between 'Sandlobae' and 'Cheongshilli', 63.636. In group 3, 'Old Home' was genetically closer to 'Bartlett' than 'Kaiser Alexander(or Bosc)'. Group 3 composed of P. communis cultivars was genetically further than other two groups, P. pyrifolia cultivars and native Korean pears.

• Korean Journal of Organic Agriculture
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• v.24 no.3
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• pp.557-570
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• 2016

Growth of 6-year old 'Niitaka' pear (Pyrus pyrifolia Nakai) trees and control of insect and disease occurrences were compared between fermented soybean extracts and rain-shelter system for two years. Foliar application of fermented soybean extracts was applied at 6 times as a pre-experiment in the open-field in 2013, with a rain-shelter system in 2014. Fermented soybean extract treatment increased foliar concentrations of approximately 0.46% T-N, 0.17% K, 0.19% Ca, and 0.06% Mg in 2013 compared to the control, with similar macro-nutrients between the control and soybean extract treatment observed in 2014. Rain-shelter system increased foliar concentrations of T-N, Ca, and Mg compared to the open-field. There were no significantly different between the control and soybean extract treatment for number of leaves per fruit, leaf dry weight, phytotoxicity, and completed shoot growth on August during the two years. Rain-shelter system increased leaf dry weight and did not affect phytotoxicity in the leaves. Fruit quality parameters were mostly similar to control and soybean extract treatment for two years, with higher fruit firmness observed for soybean extract treatment. Rain-shelter system advanced 4 days of harvest dates, and increased approximately 7.0 ton fruit yield per ha, 20 g mean fruit weight, and fruit soluble solid contents compared to open-field in 2014. Soybean extract treatment little suppressed occurrence of disease and insect on the leaves and fruits in both years. Rain-shelter system increased occurrence of Venturia nashicola on the leaves and to 63.8% of Gymnosporangium asiaticum on the fruits in 2014. Strong winds and storms in May elevated relative humidity in the rain-shelter system and caused high infection of the disease occurrence, requiring for an additional green control method. Soybean extract treatment little affected tree growth and would have initiated for a long-term study to evaluate tree physiological characteristics. Rain-shelter system improved fruit productivity and advanced harvest dates, which could have been more effective facility at a Thanks Giving Day between middle and end of September.

• Korean Journal of Agricultural Science
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• v.16 no.2
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• pp.225-238
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• 1989

These studies were conducted to investigate the extraction, purification and characterization of oriental pear (Niitaka. Pyrus pyrifolia Nak.) protease, and the results obtained were as follows: 1. Oriental pear protease was effectively extracted by the method of homogenizing pear pulp with 0.7 volume of 0.1M-sodium phosphate buffer, pH 6.5 containing 5mM-cysteine, 40mM-2-mercaptoethanol and 2mM-EDTA at 10,000 rpm for 5 min. 2. The protease was purified by ammonium sulfate fractionation, Sephadex G-100 filtration and DEAE-Sephadex A-50 column chromatography, and the purified enzyme gave a single protein band on polyacrylamide gel electrophoresis. 3. The specific activity of purified enzyme was 29.65 unit/mg protein and the yield was 7.22%. 4. The moecular weight of the protease was estimated to be about 51,000 by SDS-polyacrylamide gel electrophoresis, and the enzyme had Km value of 54.5 mg/ml for casein. 5. The purified enzyme had a maximum activity at pH 6.0 and $50^{\circ}C$, and was stable from pH 5.5-6.5 and at temperatures below $50^{\circ}C$ 6. Casein was a better substrate for this protease compared to hemoglobin. 7. The enzyme activity was markedly inhibited by p-chloromercuribenzoic acid and heavy metal salts such as $HgCl_2$ and $MnSO_4$ also considerably inhibited the enzyme activity.

• Journal of Plant Biotechnology
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• v.42 no.1
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• pp.25-33
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• 2015

A PyrcpPR-10 gene with differentially expressed was isolated by using the suppression subtractive hybridization assay between '93-3-98' (highly resistant against scab caused by Venturia nashicola) and 'Sweat Skin'(highly susceptible) and analyzed the expression pattern according to organs and cultivars. The full length of PyrcpPR-10 was cloned as 743bp with 480bp's ORP, and was determined to encode a protein of 159 amino acid residues. On analyzing PyrcpPR-10 gene sequence compared with resistant and susceptible cultivars, 'Hwangsilri' (resistant), 'Gamcheonbae' (moderately resistant), 'Wonhwang' (moderately susceptible), 'Niitaka' (highly susceptible), and 'Sweat Skin' (highly susceptible) had identical gene sequence but 'Bartlett' (highly resistant) showed partly different sequences. The deduced amino acid sequence showed 64 ~ 98% homology and had the GXGGXG motif to known amino acid of other plants PR-10 by the BLAST X analysis. Among several organs or tissues, petal was showed highest expression level of PyrcpPR-10 gene followed by leaf, floral axis, bud, and bark. The expression level of PyrcpPR-10 gene was dramatically increased at 24 hr after inoculation in all cultivars and also up-regulated in accordance with resistant degree of cultivars. While resistant cultivars ('Bartlett', '93-3-98', and 'Hwangsilri') induced relatively high expression level of PyrcpPR-10 gene, susceptible cultivars ('Niitaka', and 'Sweat Skin') showed low expression level. PyrcpPR-10 gene is assumed that it is directly connected with defense mechanisms to pear scab.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.60-65
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• 2001

'Shinil' pear cultivar (Pyrus pyrifolia Nakai) which was originated in a cross between 'Shinko' (non-patented, released in 1941) and 'Hosui' (non-patented, released in 1972) in 1978 was released as a middle season harvest variety. Its usual picking time coincided with 'Chuseok' season which is one of the most famous national holiday in Korea. The fruit showed high soluble solids content and good appearance. The cultivar was preliminarily selected in 1991, and its regional adaptability was evaluated in the name of 'Wonkyo Na-13' at 9 sites for four years from 1992, and finally selected and named in 1995. 'Shinil' is medium in tree vigor like 'Hosui' and spreading in tree habit as 'Niitaka', a leading cultivar in Korea, and consistently very productive. It has high resistance to black rot caused by Alternaria kikuchiana and pear necrotic spot caused by pear necrotic spot virus. Its full bloom is one day earlier than that of 'Niitaka' cultivar and harvest time is September 25 at Suwon area which is 3 days later than that of 'Hosui'. Fruit is round in shape with a deep medium stalk cavity and medium calyx basin and has attractive light yellow brown skin color. The fruit weight ranges between 300 and 400 g, which is similar to 'Chojuro', 'Shinko', and 'Hosui'. Soluble solid content is approximately at the level of 13-14 Brix, which is higher than that of 'Chojuro'. The flesh is cream-white, very juicy, and light grit with soft and fine texture.

• Journal of agriculture & life science
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• v.43 no.2
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• pp.23-30
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• 2009

The object of this study was to evaluate the effect of the gibberellin treatment on fruit enlargement and ripening promotion in 'Niitaka' pear(Pyms pynfolia). Fruit weight was similar between $GA_{4+7}$ treatments and the gibberellin-paste control, but fairly increased of fruit weight compared to non-treatment Most effective time for $GA_{4+7}$ treatment to increase diameter and length was on 35 and 40 days after full bloom, respectively. Shape index was similar in all treatments. Fruit enlargement at the period of early growth, $GA_{4+7}$ 2.4% treatment was remarkably effective than $GA_{3}+GA_{4+7}$ 2.7% treatment or non-treatment However at the period of maturity, $GA_{4+7}$ 2.4% treatment and $GA_{3}+GA_{4+7}$ 2.7% treatment showed little differences in fruit enlargement and coloring. In maturing promotion effect, young fruit treated with $GA_{3}+GA_{4+7}$ showed similar fruit coloring to ethephon treatment on 35 days after full bloom, and both of those treatments promoted fruit coloring than non- treatment about 6 days. $GA_{3}+GA_{4+7}$ treatments resulted higher solid content and decreased acidity than non-treatment However, there was no differences in fruit hardness comparing to non-treatment As a result, gibberellin was most effective in fruit enlargement, so as all $GA_{3}+GA_{4+7}$ treatments were more effective on fruit enlargement than ethephon treatment or non-treatment Specifically, when $GA_{3}+GA_{4+7}$ was treated 35days after full bloom of flower, the diameter, the length and the shape of fruit index were best, and fruit coloring was good as well.

• Korean Journal of Organic Agriculture
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• v.27 no.1
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• pp.33-44
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• 2019

This study was initiated to find possibility of substitute of gibberellin acid and to prevent negative girdling effect such as slow growth of 'Niitaka' (Pyrus pyrifolia Nakai) trees, a major pear cultivar, at harvest and at 60-, 90-, and 120-d after storage. Seasonal wiring with 3.0-mm-diameter were applied on the main branch at 70, 80, 90, 110, and 130 days after full bloom (DAF) to compare the fruit quality and storability. Fruit weight and sugar contents were the greatly increased by the wiring treatment at 110 DAF, with the lowest values observed for the wiring at 70 DAF. All the wiring treatments reduced fruit acidity but did not affect fruit firmness. a-value on fruit skin was the highest for wiring at 110 DAF and 130 DAF, advancing fruit ripening. The lowest fruit weight loss was occurred at wiring at 110 DAF. a-value on fruit skin was the highest for wiring at 90 DAF and 110 DAF. Wiring at 80 DAF the most increased floral bud diameter, resulting in the lowest L:D ratio of 1.74 observed. As for the mineral nutrients concentrations of floral buds, the concentration of K (0.63%) and Mg (0.31%) were the highest after 80 DAF wiring treatment, whereas Ca and P concentrations did not differ among treatment plots. The leaf K concentrations was the highest in the 130-day wiring treatment (0.76%) and in the control plot (0.78%), whereas there was no significant difference in the concentrations of either Ca or P among treatment plots. Short periods of wiring increased foliar Mg concentrations. In floral buds, carbohydrates showed a tendency for accumulating at a lower concentrations (7.75%) after 70 DAF wiring treatment, which was a relatively short treatment period. On the other hand, the carbohydrate concentrations in leaves showed a tendency for accumulating at lower concentrations after 130 DAF wiring treatment (2.51%), which is a long treatment period, and in the control plot (2.43%). Wiring increased the size and sugar content of fruits, and influenced fruit storability. For the wiring treatment period was delayed, the carbohydrate concentration in flower buds showed curvilinear correlation and the negative correlation with the carbohydrate in leaf tissue.