• Journal of Plant Biology
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• 제31권4호
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• pp.309-315
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• 1988

Leaf mesophy11 protoplast cultures from six Nicotiana species, N. debneyi, N. rustica, N. amplexicaulis, N. glauca, N. glutinosa, and N. sylvestris were carried out. When we reduced the NH4NO3 and Fe.EDTA concentration to 1/3(7 mM) and 1/10(10$\mu$M) from the Murashige and Skoog medium respectively, cell division of the protoplasts was efficiently induced in four Nicotiana species, N. debneyi, N. rustica, N. amplexicaulis and N. glauca. However, other two species, N. glutinosa and N. sylvestris were failed in inducing cell division at the same culture condition. The protoclone calluses derived from four Nicotiana species were consequently regenerated on a MS basal medium supplemented with the appropriate auxin and cytokinin.

• Journal of Plant Biology
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• 제18권4호
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• pp.150-154
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• 1975

Four kinds of Nicotiana species and five varieties belonging to N. tabacum were used as materials for electrophoretic analysis of the tetrazolium oxidase isozyme to examine the taxonomic affinity among them based on the biochemical property. All the five verieties of N. tabacum showed same isozyme bands despite the fact that these varieties had notably varied characteristics including morphological traits. The band patterns were more or less different among the four species. Although N. rustica and N. tabacum were of the same genome group of AABB, their isozyme bands showed considerable difference. N. sylvestris, genome A donor of Nicotiana species, was found to be markedly different from N. tatacum in band pattern, including the absence of system 2 in N. sylvestris.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1990년도 추계 학술대회지
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• pp.42-43
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• 1990

• BMB Reports
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• 제40권1호
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• pp.107-112
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• 2007

To study the functioning of HSP70 in Escherichia coli, we selected NtHSP70-2 (AY372070) from among three genomic clones isolated in Nicotiana tabacum. Recombinant NtHSP70-2, containing a hexahistidine tag at the amino-terminus, was constructed, expressed in E. coli, and purified by $Ni^{2+}$ affinity chromatography and Q Sepharose Fast Flow anion exchange chromatography. The expressed fusion protein, $H_6NtHSP70$-2 (hexahistidine-tagged Nicotiana tabacum heat shock protein 70-2), maintained the stability of E. coli proteins up to 90$^{\circ}C$. Measuring the light scattering of luciferase (luc) revealed that NtHSP70-2 prevents the aggregation of luc without ATP during high-temperature stress. In a functional bioassay (1 h at 50$^{\circ}C$) for recombinant $H_6NtHSP70$-2, E. coli cells overexpressing $H_6NtHSP70$-2 survived about seven times longer than those lacking $H_6NtHSP70$-2. After 2 h at 50$^{\circ}C$, only the E. coli overexpressing $H_6NtHSP70$-2 survived under such conditions. Our NtHSP70-2 bioassays, as well as in vitro studies, strongly suggest that HSP70 confers thermo-tolerance to E. coli.

• 한국환경과학회지
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• 제3권2호
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• pp.141-155
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• 1994

담배(Nicotiana tabacum)와 까마중(Solanum migrum)의 솩간 원형질체 융합으로 유도된 켈러스를 재료로하여 캘러스의 전체 단백젤과 틸라코이드막 단백질의 양태변화를 중심으로 식물생장 조절물질과 단색광의 생리적 상호효과를 조사하였다. 여러 단색 광선을 캘러스에 조사하였을때, 적색 및 청색광이 캘러스의 전체 단백질과 킬라코이드막 단백질의 합성을 촉진하였으며 , NAA+$ extrm{GA}_3$ 와 NAA+BA의 조합구에서 캘러스의 전체 단백질과 틸라코이드막 댄백질의 축척이 활발히 일어났으며, NAA+$ extrm{GA}_3$처리구에서 더욱 효과적 이였다. NAA+$ extrm{GA}_3$ 처리구에 청색광, 적색광 및 근적외광을 제각각 처리하였을때 캘러스의 전체 단백질과 킬라코이드막 단백질의 합성은 적색광에 의하여 가장 촉진되었다. 따라서 적색광과 NAA+$ extrm{GA}_3$구의 동시처리가 캘러스의 전체 단백질 및 킬라코이드막 단백질의 합성을 상승적으로 촉진함을 보였다.

• 한국연초학회지
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• 제13권2호
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• pp.52-58
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• 1991

Interspecific cross between Nicotiana trigonophylla and N. tabacum cv. BY4 is highly sterile because of abnormal ovule and embryo development. In vitro culture of excised N. trigonophylla ovules after polination by N. tabacum allows significant numbers of hybrid embryos to develop into mature plants. Total yield of seedlings and number of normal seedlings were produced following in vitro culture of individual fertilized ovules of N. trigonophylla X N. tabacum at four days post-pollination on B5 medium containing 6% sucrose. Hybrids were uniform in morphology and peroxidase isozyme composition and the majority were cytologically stable: flower characteristics were generally intermediate between those of the parents. All hybrids evaluated were self-sterile.

• 한국연초학회지
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• 제1권1호
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• pp.46-50
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• 1979

4種의 Burley種 細胞質的 雄性不稔系統과 稔性品種의 특성을 圃場에서 검정하였던 바, 雄性不稔系統들의 農耕的 化學的 특성이 稔性品種과 近似하여 , Fl 雜種 이용을 위한 交配母本으로 사용할 수 있을 것으로 考察된다.

• Journal of Plant Biology
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• 제30권1호
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• pp.69-77
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• 1987

From the single cell cultures of haploid tobacco (Nicotiana tabacum L. cv. NC 2326) glyphosate-resistant plants were regenerated. After treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and then inoculated onto the LS medium supplemented with 1 mM glyphosate, the single cells survived formed colonies and calluses in 65 and 95 days after culture, respectively, and then whole plants were regenerated in 0.1 mM glyphosate-containing medium from the selected calluses. There was no difference in fresh weight and shikimate content between the selected and normal haploid calluses. When sprayed with 0.1 mM glyphosate, the shikimate contents in the regenerated and normal plants were 0.659 and 20.816 mol/g fr. wt., while that in other normal plants which were not sprayed was 0.921. In addition, the calluses induced from the regenerated plants grew without showing any retardation when treated with glyphosate. These results indicate that the secelcted calluses and regenerated plants are resistant to glyphosate.

• Journal of Microbiology and Biotechnology
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• 제16권5호
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• pp.673-677
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• 2006

A perfusion culture of transgenic Nicotiana tabacum cell suspensions, transformed to express recombinant glucuronidase (GUS), was successfully performed in a 5-1 stirred tank bioreactor. With 0.1 $day^{-1}$ of perfusion rate, the maximum dry cell weight (DCW) reached to 29.5 g/l in 16 days, which was 2.1-fold higher than the obtained in batch culture (14.3 g/l). In terms of the production of GUS, the volumetric activity could be increased up to 12.8 U/ml by using perfusion, compared with 4.9 U/ml in batch culture. The specific GUS activities in both perfusion and batch cultures were maintained at similar levels, 200-400 U/g DCW. Consequently, a perfusion culture could be a good strategy for the enhanced production of recombinant proteins in a plant cell culture system.

• 한국연초학회지
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• 제4권1호
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• pp.21-27
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• 1982

Six flue-cured cultivars of Nicotiana tabacum L., 15 possible $F_1$ hybrids and 15 $F_2$ populations among them, and 15 haploid populations of $F_1$ hybrids and 6 haploid from parents, were evaluated. The variances of general combining ability were predominant for all characters studied in $F_1$ hybrid and $F_2$ population, and in haploid populations of $F_1$ hybrids except leaf width, value and reducing sugar. There were significant correlations between generations in variances and ranks of parental GCA effects estimated from general combining ability analysis of $F_1,\;F_2$ and haploid generation of $F_1$ hybrids for yield, stem height, days to flower. leaves per plant, value and total alkaloids.