Evaluation of Nutrients for the Protoplast Culture of Genus Nicotiana

담배 야생종의 원형질체 배양에 미치는 무기영양소의 효과

Leaf mesophy11 protoplast cultures from six Nicotiana species, N. debneyi, N. rustica, N. amplexicaulis, N. glauca, N. glutinosa, and N. sylvestris were carried out. When we reduced the NH4NO3 and Fe.EDTA concentration to 1/3(7 mM) and 1/10(10$\mu$M) from the Murashige and Skoog medium respectively, cell division of the protoplasts was efficiently induced in four Nicotiana species, N. debneyi, N. rustica, N. amplexicaulis and N. glauca. However, other two species, N. glutinosa and N. sylvestris were failed in inducing cell division at the same culture condition. The protoclone calluses derived from four Nicotiana species were consequently regenerated on a MS basal medium supplemented with the appropriate auxin and cytokinin.