• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(1)
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• pp.457-460
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• 2000

The effect of code selection for a multicode DS/CDMA system is evaluated for a high deta rate transmission, The performance is evaluated in terms of bit error and outage probabilities. The multipath fading channel is modeled as a Nakagami-m distribution which has been known to be appropriate to model the multipath fading in urban as well as indoor channels. From simulation results, it is shown that the concatenated sequence of Walsh code and Gold sequence is most promising among many code selections. The considerations in this paper can be applied to the next-generation mobile communication systems such as IMT-2000 which requires high bit rate transmissions.

• ALGAE
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• 제31권2호
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• pp.137-154
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• 2016

Next generation sequencing (NGS) technologies have revolutionized many areas of biological research due to the sharp reduction in costs that has led to the generation of massive amounts of sequence information. Analysis of large genome data sets is however still a challenging task because it often requires significant computer resources and knowledge of bioinformatics. Here, we provide a guide for an uninitiated who wish to analyze high-throughput NGS data. We focus specifically on the analysis of organelle genome and metagenome data and describe the current bioinformatic pipelines suited for this purpose.

• Journal of Plant Biotechnology
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• 제46권2호
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• pp.79-87
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• 2019

One of wild diploid Solanum species, Solanum chacoense, is one of the excellent resources for potato breeding because it is resistant to several important pathogens, but the species is not sexually compatible with potato (S. tuberosum) causing the limitation of sexual hybridization between S. tuberosum and S. chacoense. Therefore, diverse traits regarding resistance from the species can be introgressed into potato via somatic hybridization. After cell fusion, the identification of fusion products is crucial with molecular markers. In this study, S. chacoense specific markers were developed by comparing the chloroplast genome (cpDNA) sequence of S. chacoense obtained by NGS (next-generation sequencing) technology with those of five other Solanum species. A full length of the cpDNA sequence is 155,532 bp and its structure is similar to other Solanum species. Phylogenetic analysis resulted that S. chacoense is most closely located with S. commersonii. Sequence alignment with cpDNA sequences of six other Solanum species identified two InDels and 37 SNPs specific sequences in S. chacoense. Based on these InDels and SNPs regions, four markers for distingushing S. chacoense from other Solanum species were developed. These results obtained in this research could help breeders select breeding lines and facilitate breeding using S. chacoense in potato breeding.

• 식물분류학회지
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• 제53권3호
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• pp.237-241
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• 2023

The genus Scrophularia L. (Scrophulariaceae) comprises 200-270 species worldwide and is a taxonomically challenging lineage, displaying morphological diversity and hybridization. S. kakudensis is morphologically similar to the closely related taxa S. kakudensis var. microphylla, S. pilosa, and S. cephalantha. Therefore, the purpose of this study was to sequence the chloroplast (cp) genome of S. kakudensis using next-generation sequencing and compare it to those of related taxa. The complete cp genome sequence of Scrophularia kakudensis was found to be 152,355 bp long, consisting of a pair of inverted repeats of 25,485 bp that separate a large single-copy (LSC) of 83,479 bp from small single-copy regions of 17,909 bp. The cp genome contained 78 protein-coding genes, 30 tRNAs, and four rRNAs. A phylogenetic analysis based on 78 protein-coding genes from six Scrophularia species showed S. kakudensis and S. cephalantha formed with 100% bootstrap values. We compared the complete cp genomes of S. kakudensis and S. cephalantha and identified seven sequence divergence regions: matK/rps16, rps16/trnQ, trnS/trnG, rpoB/trnC, trnS/trnG, rpl32/trnL, and ndhD/psaC. These regions may be useful for determining the phylogenetic relationships among S. kakudensis-related species.

• 한국위성정보통신학회논문지
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• 제8권1호
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• pp.8-13
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• 2013

다양하고 많은 발사체를 효과적으로 운영하기 위해서 우리의 실정에 맞는 차세대 FTS(Flight Termination System)를 선정해서 개발할 필요가 있다. 차세대 FTS 방식으로는 Standard 톤, Secure 톤, MHA(Modified High Alphabet), EFTS(Enhanced FTS) 그리고 DSSS(Direct Sequence Spread Spectrum)가 검토되고 있다. FTS는 사용 목적 때문에 우수한 성능과 높은 신뢰성을 필요로 하며, 소요 수량이 적은 반면에 가격은 고가이다. 때문에 차세대 FTS의 방식을 선정해서 새로운 FTS를 도입하더라도 현재 사용하고 있는 시스템의 주요 구성품의 재사용 가능성 여부도 검토할 필요가 있다. 특히 송신기의 주요 구성품인 전력증폭기는 반드시 재사용 가능성을 검토할 필요가 있다. 신호의 CCDF(Complementary Cumulative Distribution Function)는 신호의 평균전력에 대한 첨두전력의 비를 빈도로 나타낸 것이므로 전력증폭기나 송신기의 성능을 나타낼 때 주로 사용한다. 본 논문에서는 전력증폭기의 재사용 가능성을 검토하는 방법으로 송신신호의 CCDF를 시뮬레이션해서 비교하는 방법을 이용하였다. CCDF 시뮬레이션 결과 Standard 톤의 PEP(Peak Envelop Power)는 0.21dB로 계산되었으며, Secure 톤과 MHA은 송신신호가 Standard 톤과 같기 때문에 동일한 PEP를 갖는다. CPFSK 변조를 이용하는 EFTS의 경우에 PEP는 1.81dB 였으며, BPSK 변조를 이용하는 DSSS의 경우에는 2.6dB로 계산되었다.

• Journal of Plant Biotechnology
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• 제39권4호
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• pp.273-280
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• 2012

Differences of gene expression between red flash peach cultivar and white flash peach cultivar were investigated by Nest-generation sequencing (NGS). EST from the red flash peach cultivar and white flash peach cultivar were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, temperature stress, ethylene signal pathway were significantly higher in white flash peach cultivar than in red flash peach cultivar. On the other hand, the up-regulation of proteins involved in anthocyanin and flavonol biosynthesis and protein degradation and sorbitol metabolism were observed in red flash peach cultivar. Chalcone synthase was preferentially expressed in the red flesh peach cultivar, agreeing with the accumulation of anthocyanin and expression of other previously identified genes for anthocyanin biosynthesis. Anthocyanin pathway related genes CHS, F3H, DFR, LDOX, UFGT differentially expressed between red flash peach cultivar and white flash peach cultivar. These results suggest that red flash peach cultivar and white flash peach cultivar have different anthocyanin biosynthesis regulatory mechanisms.

• 한국콘텐츠학회논문지
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• 제22권2호
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• pp.762-769
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• 2022

산전 진단에서 유전자 검사는 임상 관리 및 부모의 의사 결정에 중요한 정보를 제공하고 있다. 지난 여러 해 동안 G-banidng 핵형 분석, 형광성 제자리 교잡 방법, 염색체 마이크로어레이 및 유전자 패널과 같은 세포유전학적 검사 방법들이 일반적인 산전 진단의 검사의 일부가 되어 발전해 왔다. 그러나 이러한 각각의 방법은 한계를 가지고 있으며 각각의 진단 기술의 단점들을 보완할 수 있는 혁신적인 검사 방법의 도입의 필요성이 매우 필요한 시점이다. 최근 차세대 염기서열 분석에 기반한 유전체 분석 방법의 도입은 현재의 산전 진단에서의 관행에 많은 변화를 주고 있다. 이렇게 산전 진단에서의 유전체 단위의 염기서열 분석은 정교한 해상도와 높은 정확도를 통해 데이터를 빠르게 분석하고 비용을 감소시키는 기술의 혁신을 보여주고 있다. 따라서 본 논문에서는 시퀀싱 기반 산전 진단의 현재 상태와 관련 과제 및 미래 전망에 대하여 검토해 보았다.

• 한국동물위생학회지
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• 제45권1호
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• pp.31-38
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• 2022

Swine influenza (SI) is an important respiratory disease in pigs and epidemic worldwide, which is caused by influenza A virus (IAV) belonging to the family of Orthomyxoviridae. As seen again in the 2009 swine-origin influenza A H1N1 pandemic, pigs are known to be susceptible to swine, avian, and human IAVs, and can serve as a 'mixing vessel' for the generation of novel IAV variants. To this end, the emergence of swine influenza viruses must be kept under close surveillance. Herein, we report the isolation and phylogenetic study of a swine IAV, A/swine/Korea/21810/2021 (sw21810, H3N2 subtype). BLASTN sequence analysis of 8 gene segments of the isolated virus revealed a high degree of nucleotide similarity (94.76 to 100%) to porcine strains circulating in Korea and the United States. Out of 8 genome segments, the HA gene was closely related to that of isolates from cluster I. Additionally, the NA gene of the isolate belonged to a Korean Swine H1N1 origin, and the PB2, PB1, NP and NS genes of the isolate were grouped into that of the Triple reassortant swine H3N2 origin virus. The PA and M genes of the isolate belonged to 2009 Pandemic H1N1 lineage. Human infection with mutants was most common through contact with infected pigs. Our results suggest the need for periodic close monitoring of this novel swine H3N2 influenza virus from a public health perspective.

• The Plant Pathology Journal
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• 제33권5호
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• pp.508-513
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• 2017

The complete genome sequence of a Slovak SL-1 isolate of Tomato mosaic virus (ToMV) was determined from the next generation sequencing (NGS) data, further confirming a limited sequence divergence in this tobamovirus species. Tomato genotypes Monalbo, Mobaci and Moperou, respectively carrying the susceptible tm-2 allele or the Tm-1 and Tm-2 resistant alleles, were tested for their susceptibility to ToMV SL-1. Although the three tomato genotypes accumulated ToMV SL-1 to similar amounts as judged by semiquantitative DAS-ELISA, they showed variations in the rate of infection and symptomatology. Possible differences in the intra-isolate variability and polymorphism between viral populations propagating in these tomato genotypes were evaluated by analysis of the capsid protein (CP) encoding region. Irrespective of genotype infected, the intra-isolate haplotype structure showed the presence of the same highly dominant CP sequence and the low level of population diversity (0.08-0.19%). Our results suggest that ToMV CP encoding sequence is relatively stable in the viral population during its replication in vivo and provides further demonstration that RNA viruses may show high sequence stability, probably as a result of purifying selection.

• 한국통신학회논문지
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• 제33권5A호
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• pp.479-486
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• 2008

본 논문은 MIMO-OFDM 시스템을 기반으로 한 Gbps급 차세대 무선전송 시스템에서 m-sequence를 이용하여 효율적으로 훈련심볼을 설계하는 방법을 제안한다. 계산량을 고려하지 않는 일반적인 훈련심볼 설계방법에는 랜덤한 시퀀스에 대하여 시스템 요구사항을 하나하나 비교해가며 모든 사항이 만족되었을 때 훈련심볼로 채택하는 기법이 존재한다. 본 논문에서는 상관관계 특성이 우수하여 대역확산 통신 방식 등에서 사용하는 m-sequence를 기반으로 제한된 탐색공간 내에서 효율적인 MIMO-OFDM 시스템을 위한 훈련심볼의 단계별 설계 및 검증방법을 논의한다. 제안된 방법은 패킷 기반 MIMO-OFDM 무선통신 시스템에서 자동이득제어, 타이밍 동기, 주파수 및 시간옵셋 추정, MIMO 채널추정 등을 포함한 시스템에서 요구하는 조건을 만족시키는 훈련심볼 설계를 목표로 한다.