• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.480-489
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• 2020

Objective: Sow milk (SM) may not be able to meet the piglet's nutritional needs in late lactation. Hence, this study was conducted to investigate the effects of early commercial milk (CM) supplement on the mucosal morphology, bacterial community and bacterial metabolites in jejunum of piglets. Methods: Ten litters of newborn piglets ([Yorkshire×Landrace]×Duroc) were randomly divided into 2 groups of 5 litters. The piglets in the control group were suckled by the sow (SM), while the piglets in the treatment group (CM supplement) were supplemented with a CM supplement along with suckling from d 4 to d 28 of age. Results: No significant differences were observed about jejunal mucosal morphology on d 28 and d 35 between two groups. On d 28, the activity of lactase in the jejunum was significantly decreased in the CM group, while the activity of sucrase and the ratio of maltase to lactase were significantly increased (p<0.05). On d 35, the activity of maltase in the jejunum was significantly increased in the CM group (p<0.05), and maltase to lactase ratio tended to increase in the CM group (p = 0.065). In addition, piglets in the CM group had a higher abundance of Clostridium XI, Tuicibater, and Moraxella in the jejunum on d 28, while the abundance of Lactobacillus was significantly increased on d 35 (p<0.05). Conclusion: The early CM supplement improved the maturation of the jejunum to some extent by enhancing the maltase and sucrase activities. Moreover, the early CM supplement could help maintain the homeostasis of internal environment in jejunum by increasing the microbial-derived metabolites.

• Korean Journal of Veterinary Research
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• v.30 no.3
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• pp.333-341
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• 1990

To investigate bovine leukosis virus (BLV) infection in the cattle rearing in a dairy farm where a case of bovine lymphosarcoma had been identified several years ago, the 196 Holstein cattle including newborn calves to 12 years of age were tested. The BLV antibody test and peripheral lymphocyte count for bovine leukosis were carried out by the immunodiffusion (ID) test and Bendixen's Kep. These tests were performed 2 to 4 times at the interval of 3 to 5 months. The observed results were as follows: 1. The positive rates of BLV-antibody in the 1st, the 2nd, the 3rd and the 4th tests were 23.3%, 28.1%, 49.0% and 55.7%, respectively. The conversion rates from negative to positive in the 2nd, the 3rd and the 4th tests were 8.9%, 41.4%, and 20.0%, respectively. Results showed that the highest conversion rate was observed at the 3rd test which was conducted after winter. The highest positive rate by ID test were observed in 4 year old cattle in the 1st and 2nd tests, and in 2 year old herd in the 3rd and 4th tests. 2. In hematological test by Bendixen's Key, the positive and suspicious rates in the 1st, the 2nd, the 3rd and the 4th tests were 5.8 and 7.8%, 8.3 and 6.6%, 8.7 and 10.1%, 10.8 and 19.6% respectively. Results showed that the positive and. suspicious rates increased in course of time. 3. 70 to 100% of the positive cattle in hematological test were positive for BLV-antibody test. All of 13 cattle with persistent lymphocytosis (PL) were also positive for BLV-antibody, indicating the high relationship between PL and BLV-antibody. 4. The number of total leukocytes and absolute lymphocytes in the BLV-antibody positive cattle appeared significantly higher than those of the negative cattle. The markedly increased cell counts were observed in the cattle over one year old. 5. The mean of total leukocytes and absolute lymphocytes in the negative cattle for BLV-antibody increased slightly after sero-conversion into positive. 6. In the clinical examinations during experimental periods, none of the 72 positive cattle for BLV-antibody showed any lesions for bovine leukosis.

• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.187-191
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• 2007

This study was canted out to develop cell lines overexpressing human H-transferase (HT). One of the approaches to prevent hyperacute rejection in xenotransplantation might be the expression of human HT in porcine cells. In this study, we cloned human HT gene from HepG2 cells using RT-PCR to establish HT-overexpressing vector. The full-length cDNA of human HT was inserted into the 3' end of CMV promoter for construction of the overexpression vector pRc/CMV-hHT. Using ietPEI DNA transfection reagent, the vector was introduced into porcine ear skin fibroblasts from newborn piglets. Transfected cells were selected by treatment of $300{\mu}g/ml$ G418 for 12 days. After antibiotic selection, survived colonies with approximately 5mm in diameter were picked and analysed for transgene human HT by PCR. The colonies proven to be human HT transfectants were analysed by RT-PCR to determine their expressions or human HT. In all colonies tested, human HT mRNA was detected. This result demonstrates the establishment of porcine cell lines overexpressing human HT, and these cell lines may be used for the development of transgenic pigs for xenotransplantation.

• Food Science of Animal Resources
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• v.27 no.3
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• pp.337-344
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• 2007

We have investigated the relationship between food allergen sensitization and allergic disease in 74 child (male 47, female 27) patients from 0 to 14 years of age diagnosed with allergic disease. The age distribution for the study was: newborn to 3 years old, 34 children; 4 to 6 years old, 24 children; 7 to 9 years old, 8 children and above 10 years old, 8 children. Of the 74 children, 10 children were allergic to 3 of the 21 types of foods tested, 21 children were allergic to 4 types and 15 children were allergic to 5 types. The results of specific IgE tests for class 2 (0.070-3.49 IV/mL, IgE density in serum) showed that 29 children were allergic to milk, 28 children to bean, 21 children to cheese, 7 children to egg, and 18 children to pork, while over class 2, 20 children were allergic to bean, 17 children to milk, 24 children to cheese, 20 children to egg, and 21 children to pork. A questionnaire was used to survey family allergy history and diet patterns for 40 child (male 22, female 18) patients with allergic disease. The frequencies of a family history of allergy were 45.5% for males and 50.0% for females. The allergic diseases included atopic dermatitis: 26.0%, atopic nasitis: 10.5%, atopic dermatitis + atopic nasitis : 31.5%, hives: 21.0%, and asthma: 10.5%. Children on diets of mixed breast feeding and infant formula were more allergic than those on either breast feeding or infant formula feeding. Eliminated allergenic foods were egg + milk: 12.5%, egg: 10.0%, and milk 2.5%.

• Journal of Nutrition and Health
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• v.27 no.9
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• pp.930-939
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• 1994

Vitamin B-6 nutrition has been shown to be inadequate in many population groups including pregnant and lactating women, and in infants. Vitamin B-6 intake was measured in 98 pregnant mothers and a total of 172 cord blood samples of their and other new born infants were analyzed for erythrocyte alanine aminotransferase(EALAT) activities with or without the addition of pyridoxal-5-phosphate to assess vitamin B-6 status of the infants. The average daily vitamin B-6 intake of the pregnant mothers was 1.79mg$\pm$0.88(81.4% of the Recommended Dietary Allowances ; RDA) and vitamin B-6 to protein intake ration was 0.017mg vitamin B-6/g protein. Thirty-eight percent of the pregnant women consumed diets which provided less than the RDA for vitamin B-6 during pregnancy. Seventy-two percent of the dietary pyridoxine intake was provided by the plant food source whose bioavailability was reported to be lower when compared to that of the animal food. The average activity coefficient(AC) values of the cord blood EALAT was 1.41$\pm$0.11, and 32% of the blood samples had EALATAC values greater than 1.25, suggesting that vitamin B-6 status of the newborns might be less than adequate.

• Korean Journal of Veterinary Research
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• v.33 no.2
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• pp.301-308
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• 1993

Three or 7day old piglets were infected experimentally with different encephalomyocarditis virus isolates to detect the viral antigen by the immunoperoxidase technique and to observe strain difference in their pathogenecity in newborn pigs by comparing clinical signs and pathologic lesions. Clinical signs of the infected pigs were different depending on the virus strain, pig age and infection route. Encephalomyocarditis virus(EMCV) NVSL-PR isolate was more pathogenic than MN-25 and MN-30 isolate. Three day old piglets showed more severe illness than 7 day old piglets. Predominant clinical signs were sudden death without noticeable clinical signs and dyspnea manifested as heavy abdominal breathing. Contact-infection from infected piglets to controls was observed in the oro-nasally infected group but not the intramuscular group. Common necropsy findings of dead piglets in both age groups infected with MN-25 and NVSL-PR were accumulation of excessive fluid in the body cavities and mild to diffuse necrotic areas observed in the hearts and occasionally in the livers. Microscopically, myocarditis with inflammatory cell infiltration, necrosis of the myocardial muscle fibers and occasional mineralization were observed along with interstitial pneumonia and centrolobular necrosis in the liver. Using an immunoperoxidase technique, viral antigen was detected in myocardial muscle fibers of piglets infected with EMCV.

• Advances in pediatric surgery
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• v.17 no.2
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• pp.179-187
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• 2011

Experimental tracheal ligation (TL) has been shown to reverse the pulmonary hypoplasia associated with congenital diaphragmatic hernia (CDH) and to normalize gas exchange. The purpose of this study was to determine whether the TL would correct the surfactant deficiency present in the fetal rabbit model of CDH by using lamellar body count. Lamellar bodies are synthesized and secreted by the type II pneumocytes of fetal lung. The phospholipids present in these bodies constitute the major component of pulmonary surfactant. Twenty-one pregnant New Zealand rabbits underwent hysterotomy and fetal surgery on gestational day 24. Two fetuses of each pregnant rabbit were operated. In the fetus of one end of bicornuate uterus, left DH was created by excision of fetal diaphragm through open thoracotomy (DH Group). In the fetus of the other end of bicornuate uterus, left DH and TL were created (TL Group). The fetuses were delivered by Cesarean section on gestational day 31. Fourteen in control group, 12 in the DH group and 13 in TL group were born alive. En bloc excision of lungs, bronchi and trachea was done in all newborn rabbits. A five Fr catheter was inserted through trachea and repeated irrigations with 10 cc normal saline were done. The irrigated fluid was centrifuged at $280{\times}g$ for 5 minutes and the lamellar bodies were counted with the upper level fluid in platelet channel of electronic cell counter. The average lamellar body counts were $37.1{\pm}14.2{\times}10^3/{\mu}L$ in control group, $11.5{\pm}4.4 {\times}10^3/{\mu}L$ in DH group, and $6.5{\pm}0.9{\times}10^3/{\mu}L$ in TL group. Lamellar body count in DH group was lower than in control group and did not increase after TL. This study shows TL has no therapeutic effect on decreased surfactant level of CDH and the pregnant rabbit is appropriate for the animal model of CDH.

• Journal of Dairy Science and Biotechnology
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• v.35 no.2
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• pp.105-111
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• 2017

There have been numerous reports indicating that milk proteins influence immune functions. Colostrum refers to the breast milk of mammals, secreted starting from the fourth or fifth day after delivery. It has abundant nutrition for the survival of newborn infants. Most importantly, it contains bioactive substances with growth-stimulating and antibiotic, functions. Thus, the colostrum has various physiological roles. This study measured the differences in the composition of colostrum derived from dairy cattle, hanwoo, porcine, and goat sources. The results showed that immunoglobulin, lactoferrin, lactoperoxidase, serum albumin, IgG heavy chain, and IgG light chain were significantly higher in the colostrum of dairy cattle, hanwoo, and goats, but low in porcine colostrum. There was no significant difference in ${\alpha}_{S2}$-casein, ${\alpha}_{S1}$-casein, ${\beta}$-casein, ${\kappa}$-casein, ${\beta}$-lactoglobulin, and ${\alpha}$-lactalbumin contents until seven days after birth. However, porcine colostrum showed high contents of all proteins from the first day to the second day after delivery.

• Journal of Animal Science and Technology
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• v.63 no.5
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• pp.1114-1125
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• 2021

Supplying a sufficient amount of high-quality colostrum is a prevailing challenge in Hanwoo calf production systems. This study aimed to describe the efficiency of a lacteal-derived colostrum replacer versus natural bovine colostrum (from Holstein) fed to Hanwoo calves. Forty newborn Hanwoo calves (25 males and 15 females; 30.7 ± 3.15 kg body weight [mean ± standard deviation]) were blocked by sex and birth weight and fed either natural colostrum or a commercial colostrum replacer. Calves displayed a narrow difference in the evolution of body weight and structural body dimensions when they received a commercial versus natural colostrum. Minor differences existed in the starter and total dry matter consumption between calves fed natural or commercial colostrum. Although the colostrum source had no significant effects on days to first diarrhea (average of 7.6 days; p = 0.17), the duration of diarrhea was longer in natural colostrum-fed calves (3.2 vs. 4.5 days; p = 0.04). Consumption of natural colostrum resulted in greater mean serum immunoglobulin G (IgG) on day 2 (14.7 vs. 10.8 ± 0.92 mg/mL) and day 7 (8.21 vs. 5.12 ± 0.82 mg/mL) of calf life, resulting in a greater proportion of commercial colostrum-fed calves (50% vs. 15%; day 2) to experience failure of passive transfer of immunity (serum IgG < cutoff point of 10 mg/mL). Overall, growth rate, body frame development, and incidence of diarrheal disease were not different in both groups. These results suggested that the colostrum replacer product tested in this study could be an alternative to natural colostrum derived from Holstein cow in securing calf growth and health in Hanwoo calf-rearing operations.

• Journal of the Korea Institute of Military Science and Technology
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• v.24 no.3
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• pp.348-355
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• 2021

Hantaan virus(HTNV) causes hemorrhagic fever with renal syndrome(HFRS) with a case fatality rate ranging from <1 to 15 % in human. Hantavax^Ⓡ is a vaccine against the Hantavirus, which has been conditionally approved by the Ministry of Food and Drug Safety(MFDS). However, only 50 % of volunteers had neutralizing antibodies 1 year following the boost. Effective antiviral treatments against HTNV infection are limited. Hantaviruses generally cause asymptomatic infection in adult mice. On the other hand, infection of suckling and newborn mice with hantaviruses causes lethal neurological diesease or persistant infection, which is different from the disease in humans. The development of vaccines and antiviral strategies for HTNV has been partly hampered by the lack of an efficient lethal mouse model to evaluate the efficacy of the candidate vaccines or antivirals. In this report, we established a lethal mouse model for HTNV, which may facilitate in vivo studies on the evaluation of candidate drugs against HTNV. The median lethal dose value of HTNV was calculated by probit analysis of deaths occurring within two weeks. Five groups of ten ICR mice were injected intracranially with serial 2-fold dilutions (from 50 to 3.125 PFU/head) of HTNV. Mice injected with HTNV began to die at 8 days post-infection. The lethal dose required to kill 50 % of the mice (LD₅₀) was calculated to be 2.365 PFU/head.