• Molecules and Cells
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• v.19 no.3
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• pp.391-397
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• 2005

Molecular identification techniques are used where morphological characters are not useful for distinguishing species that resemble each other closely. The example studied here is the Adoxophyes species complex, in which A. orana (Fischer von $R{\ddot{o}}sslerstamm$) is officially the only known Korean species in the genus Adoxophyes (Lepidoptera: Tortricidae). However there have been suspicions that at least two types of A. orana exist in Korea based on the distribution and range of the host, with A. orana attacking apples and peaches, and another Adoxophyes sp. attacking tea and pears. The latter is presumed to be A. honmai (Yasuda), but the two have remained confused because of their extreme morphological similarity, despite several Asian studies of pheromonal and morphological characteristics. To confirm the occurrence of an Adoxophyes species other than A. orana in Korea, we compared 940 bp of the mitochondrial cytochrome oxidase I (COI) gene from 16 samples of Adoxophyes and found that there is a second Adoxophyes species different from A. orana. Comparison of the different sequences to that of Japanese A. honmai confirmed that they belong to the latter. From the sequence difference between the two Korean species, we were able to develop new PCR primer sets that distinguish them. This molecular identification technique with no enzyme digestion or sequencing step is a convenient and rapid way of differentiating between species that are hard to distinguish morphologically.

• Animal Systematics, Evolution and Diversity
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• v.29 no.4
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• pp.288-293
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• 2013

A single specimen of the genus Sillago, collected from Gwangyang, Korea, in May 2009, is characterized by XI first dorsal fin spines, 3 or 4 rows of melanophore pattern along the second dorsal fin membrane, and a darkish posterior margin of the caudal fin. Our specimen was identified as Sillago sinica reported as a new species; this identification is confirmed by mitochondrial DNA cytochrome oxidase subunit I sequences, which show that our specimen corresponds to S. sinica (d=0.000) and differs from the congeneric species Sillago parvisquamis (d=0.170). Comparisons of Korean specimens previously reported as S. parvisquamis with specimens of S. sinica show that the S. parvisquamis specimens are actually S. sinica. We propose the new Korean name "buk-bang-jeom-bo-ri-myeol" for S. sinica.

• Korean Journal of Plant Taxonomy
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• v.50 no.1
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• pp.1-7
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• 2020

Cynanchum L. in the subfamily Asclepiadoideae (Apocynaceae) includes four recognized species on the Korean peninsula, two of which are native to South Korea. However, the species ranges in South Korea are poorly defined. During a field survey, we discovered C. thesioides, previously unrecorded in South Korea, in Gimpo-si, Gyeonggi-do, and found an additional population of C. chinense, for which only one population has been reported in South Korea. The two taxa are considered rare species with extremely restricted distributions in South Korea, especially C. thesioides. We provide fundamental information, including descriptions, images of the habitats and morphological characters, and a taxonomic key for identification and assessments of the conservation status of Cynanchum species in Korea.

• Journal of Species Research
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• v.5 no.2
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• pp.220-222
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• 2016

An Uraniidae moth, Lyssa zampa (Butler, 1869) is newly discovered from southern Korea. One male specimen was collected at a valley of south aspect of Mt. Hallasan, Jejudo in July, 2014. This species is briefly described and illustrated here. DNA barcoding sequence (Genbank KU160388) for the species was provided for species identification.

• Journal of the Korean Wood Science and Technology
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• v.18 no.2
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• pp.49-66
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• 1990

In order to identify an unknown wood sample native to Korea. the softwood databases(KSWCHUN; Korean SoftWood CHUN) and the hardwood databases(KHWCHUN; Korean HardWood CHUN) had been built. and the new computer searching programs(IDINEX; IDentification INformation EXpress) has been written in Turbo Pascal(V.5.0) and in Macro Assembly(V.5.0). The characters of the data were based on the 74 features of softwood and on the 148 features of hardwood which are a part of new "IAWA list of microscopic features for hardwood identification" published in 1989. For the purpose of this investigation the wood anatomical nature of 25 species of softwood(13 genera of 5 families) and of 112 species of hardwood(57 genera of 31 families) were observed under a scanning electron microscope and light microscope. and a lot of literature used. The IDINEX programs are based on edge-punched card keys. with several improvements. The maximum number of features in the IDINEX is 229. but that is fixed for a given database. Large numbers of taxa are handled efficiently and new taxa easily added. A search may be based on sequence numbers of features. Comparisons are made sequentially by feature and taxon using the entire suite of features specified to produce the list of possible matching taxa. The results are followings. (1) The databases of Korean wood and the searching programs(IDINEX) had been built. (2) The databases of Korean wood could be an information to search an unknown wood. (3) The databases would be valuable. for the new features, which were not mentioned in Korean wood up to the present. were observed in details. (4) The ultrastructures of the cell walls(warty layer) and crystals observed under a scanning electron microscope will be helpful to search an unknown wood in particular. (5) The searching process is more quick and accurate than the others. 6) We can obtain the information on the differences of a species from the other and search an unknown wood using probability. in IDINEX, (7) The IDINEX will be utilized to identify and classify an animal life, vegetable world, mineral kingdom, and so on.

• Journal of Species Research
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• v.4 no.2
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• pp.61-96
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• 2015

Korean species of Achyrolimonia Alexander, 1965, Discobola Osten Sacken, 1865, Elephantomyia Osten Sacken, 1860, Geranomyia Haliday 1833, Helius Lepeletier and Serville, 1828 (in Latreille et al., 1833), Libnotes Westwood, 1876 and Trentepohlia Bigot, 1854 crane flies (Diptera: Limoniidae) are taxonomically revised. Genera Elephantomyia and Trentepohlia, subgenus Libnotes (Afrolimonia) Alexander, 1965, species Achyrolimonia basispina (Alexander, 1924), Geranomyia multipuncta Alexander, 1922, Helius (Helius) nipponensis (Alexander, 1913), Libnotes (Libnotes) amatrix (Alexander, 1922), L. (L.) longistigma Alexander, 1921, and L. (L.) puella Alexander, 1925 are new records for the Korean peninsula. Genera Discobola Osten Sacken, 1865, Geranomyia Haliday, 1833 and Libnotes Westwood, 1876 are new records for South Korea. Identification keys, redescriptions and illustrations of all species and both sexes, if they were found in Korea, are presented.

• Journal of Species Research
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• v.6 no.3
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• pp.258-279
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• 2017

Korean species of Atypophthalmus Brunetti, 1911 and Thrypticomyia Skuse, 1890 crane flies (Diptera: Limoniidae) were taxonomically revised. New findings of Achyrolimonia Alexander, 1965, Helius Lepeletier and Serville, 1828 (Latreille et al., 1828) and Limonia Meigen, 1803 were discussed. Genera Atypophthalmus and Thrypticomyia, species Helius(Helius) flavus(Walker, 1856) were newly recorded for the Korean Peninsula, and Achyrolimonia neonebulosa (Alexander, 1924) and Limonia juvenca Alexander, 1935 were new records for North Korea. Male terminalia of L. episema Alexander, 1924 were described with detailed photographs. Identification keys, re-descriptions and illustrations of both sexes from all species in Korea were presented.

• Animal Systematics, Evolution and Diversity
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• v.39 no.3
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• pp.114-117
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• 2023

The genus Opius Wesmael, 1835 (Hymenoptera: Braconidae: Opiinae) generally parasitizes larvae of Agromyzidae, Anthomyiidae, Drosophilidae, Tephritidae, Ephydridae and Cecidomyiidae, of which 32 species are recorded in South Korea. In China, seven new species of the genus Opius have been known. Among them, Opius crenuliferus Li & van Achterberg, 2013 is reported for the first time in South Korea. Specimen was collected by sweeping in West Daeshin-dong, Busan, Korea. In this study, description, and diagnostic illustration of O. crenuliferus are provided.

• Animal Systematics, Evolution and Diversity
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• v.37 no.1
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• pp.26-30
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• 2021

Botrylloides violaceus is native to the Northwest Pacific, including Korea. This species has many color variations in alive condition and a variety of zooid compound forms, and therefore difficult to identification in the field survey. This is the first report of COI DNA barcodes of B. violaceus from Korea. The intra-specific pairwise distance between Korean and UK populations had ranged from 1.4% to 2.6%. The inter-specific variations between B. violaceus and other Botrylloides species were 21.0-36.8%. The new DNA barcodes for Korean B. violaceus may be helpful in the identification of colonial ascidians, which is a difficult task when based on morphological identification.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.56 no.4
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• pp.316-321
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• 2020

A single juvenile malacanthid specimen (Hoplolatilus chlupatyi) was collected from the South Sea of Korea and identified by DNA barcoding. This species is readily distinguished from other malacanthid species in having well developed rostral spine and elongated spines in the posttemporal and preopercle. A molecular analysis based on mitochondrial DNA COI sequences showed that this species is matched to adult H. chlupatyi (genetic distance = 0.005). Therefore, this is the first record of H. chlupatyi in Korea. We propose new Korean names, "Mu-ji-gae-og-dom-sog" for the genus Hoplolatilus and "Mu-ji-gae-og-dom" for the species H. chlupatyi.