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Fauna of Arthropods of Medical Importance in Chindo Island , Korea (전남 진도의 위생 절지동물상)

  • han-II Ree
    • Animal Systematics, Evolution and Diversity
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    • v.11 no.1
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    • pp.87-100
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    • 1995
  • Fauna of medically important arthropods in Chindo Island was studied in July 1994. Among eight speices of mosquitoes (Diptera : Culicidae), Culex tritenirohyunchus was predomiant (46.5% of the total0, and Anopheles sinensis the next. Seven specimens of the unidentified Culex sp. which seemed to be new were also collected. Total 23 species of non-biting midges(Diptera : Chiornomidae) were found in Chindo Is. Of them , Chironomus kiiensis was the predominant species (67.3%) , and Ch.flaviplumus the next (15.6%). Three species, Cladopelma viridula, Dicrotendipes septemmaculatus and Harnischia urtilamellata are reported for the first time in Korea, and they are fully re-described with illustrations. Among five species of biting midges (Diptera : Ceraopogenidae) collected, Culicoides punctatus was predominant (88.7%). A species of Foreipomia was also collected, and this genus is not recorded in Korean fauna. Only two species of domiciliary cockroaches, Periplaneta japonica and P.americana were collected in small numbers and Blatella germanica which is known as the most predominant speices in Korea was not found. The predominant speices of field rodents was Apodemus agrarius and thier ectoparasites were Leptotrombidium orientale (Acarina : Tromicibulidae), Lxodes nipponensis (Acarina : Ixodidae) ; Laelaps jettmani and Eulaelaps stabularis (Acarina : Laelapidae).

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Chemical Characteristics and Biological Activities of Herbimycin A and Dihydroherbimycin A Produced by a Soil Isolate Streptomyces sp. AO-0511 (Streptomyces sp. AO-0511이 생산하는 Herbimycin A 및 Dihydroherbimycin A의 이화학적 특성 및 생물 활성)

  • Chang, Hung-Bae;Kim, Se-Chan;Kim, Jae-Heon
    • Korean Journal of Microbiology
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    • v.42 no.1
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    • pp.47-53
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    • 2006
  • A streptomycete strain was isolated from the soil samples from Korea. The chemotaxonomy and 16S rDNA sequencing confirmed that the strain belonged to the genus Streptomyces and we named it Streptomyces sp. AO-0511. Two antibiotics, herbimycin A and dihydroherbimycin A produced by this strain were tested for their physico-chemical and biological characteristics. Both compounds were stable under acidic pH. Dihydroherbimycin A was more heat-stable and polar compared with herbimycin A. Only weak antibacterial activities were detected against Bacillus subtilus ATCC 6633 and Micrococcus luteus ATCC 9341. However, herbimycin A and dihydroherbimycin A showed strong inhibitory activities on lung cancer cells (A549 cells) and leukemia cells (HL-60). The cytotoxicity was determined using L5178Y and P388 cell lines. The results showed that herbimycin A and dihydroherbimycin A had lower toxic effects on the cells compared with the standard compounds, comptothecin and cyclosporin A. Therefore, both compounds could be good candidates for the development of new anticancer drugs.

Discovery of Spathius ibarakius Belokobylskij et Maeto (Hymenoptera: Braconidae) as a Larval Ectoparasitoid of Citrus Longhorned Beetle in Korea (알락하늘소 유충의 외부기생 알락하늘소살이고치벌(Spathius ibarakius Belokobylskij et Maeto)의 발견)

  • Kim, Moo-Sung;Lee, Hye-Lin;Ku, Deok-Seo;Herard, Franck;Gould, Juli R.;Williams, David W.;Kim, Il-Kwon;Hong, Ki-Jeong
    • Korean journal of applied entomology
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    • v.55 no.3
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    • pp.285-291
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    • 2016
  • Spathius ibarakius Belokobylskij et Maeto, which belongs to the Spathius exarator species group (Hymenoptera: Braconidae: Doryctinae) was found as an ectoparasitoid of the first- and second-instar larvae of the citrus longhorned beetle, Anoplophora chinensis ($F{\ddot{o}}rster$) from Gwangneung, Pocheon, Korea. The discovery of this braconid wasp was made during the survey for parasitoids of the Asian and citrus longhorned beetles by using the sentinel log method. We herein describe diagnostic characters of S. ibarakius, including those of the male, and also provide illustrations. In addition, we present a host list of Korean species belong to genus Spathius.

First Record of the Cutlass Fishes, Benthodesmus tenuis (Perciformes: Trichiuridae) from Korea (한국산 갈치과 어류 1 미기록종, Benthodesmus tenuis)

  • Kim, Maeng Jin;Choi, Jung Hwa;Oh, Tag Yun;Kim, Jung Yun;Lee, Dong Woo
    • Korean Journal of Ichthyology
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    • v.24 no.4
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    • pp.302-305
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    • 2012
  • Two specimens (332.0~377.6 mm in standard length) of Benthodesmus tenuis (G$\ddot{u}$nther), belonging to the family Trichiuridae, firstly collected in off Heuksan-do and Yeosu, Jeollanam-do by the bottom trawl. This species was characterized by the following morphological traits: 125~126 dorsal fin rays (vs. 144~155 for B. pacificus), I, 73 anal fin rays (vs. 91~101), interorbital region flat (vs. interorbital region with prominent crest for A. anzac), opercular black and caudal fin forked. We propose a new Korean name, "Se-jang-gal-chi-sok" and "Se-jang-gal-chi" for the genus and species, respectively.

First Record of a Bothid Flounder, Japonolaeops dentatus (Bothidae, Pleuronectiformes) from Jeju Island, Korea (한국산 둥글넙치과 어류 1미기록종, Japonolaeops dentatus)

  • Kim, Maeng-Jin;Choi, Jung-Hwa;Oh, Taeg-Yun;Kim, Jung-Nyun;Song, Choon-Bok
    • Korean Journal of Ichthyology
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    • v.23 no.3
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    • pp.242-245
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    • 2011
  • This is the first report of Japonolaeops dentatus Amaoka, belonging to the family Bothidae, collected in Korea. Four specimens (152.9~174.7 mm in standard length) were caught in the coastal waters of Jeju Island by using the bottom trawl. This species was characterized by the following morphological traits: 115~120 dorsal fin rays, 96~98 anal fin rays, 105~106 pores in lateral line, upper jaw extending to below anterior margin of lower eye, and teeth presenting on both ocular and blind sides of the jaw. We propose a new Korean name, "Geom-eun-kko-ri-neop-chi-sok" and "Geom-eun-kko-ri-neop-chi" for the genus and species, respectively.

First Record of the Trachichthyid Fish, Aulotrachichthys prosthemius (Beryciformes: Trachichthyidae) from Korea (한국산 납작금눈돔과 어류 1 미기록종, Aulotrachichthys prosthemius)

  • Kim, Maeng-Jin;Lee, Dong-Woo;Choi, Kwang-Ho;Oh, Taeg-Yun;Choi, Jung-Hwa
    • Korean Journal of Ichthyology
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    • v.24 no.2
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    • pp.143-146
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    • 2012
  • This is the first report of Aulotrachichthys prosthemius (Jordan and Fowler), belonging to the family Trachichthyidae, collected in Korea. One specimens (74.8 mm in standard length) were caught in the coastal waters of Jeju Island by using the bottom trawl. This species was characterized by the following morphological traits: V, 13 dorsal fins; III, 9 anal fins; the striated area along the ventral side of the body reaches to the tip of last anal rays and the post-temporal spine is sharp, pointed and with serrated edge. We propose a new Korean name, "Eeun-jool-geum-nun-dom-sok" and "Eeun-jool-geum-nun-dom" for the genus and species, respectively.

Recent Progress in Development of Vaccines against Avian Coccidiosis (조류 콕시듐증의 백신개발에 대한 최근의 진보)

  • Lillehoj, Hyun S.
    • Korean Journal of Poultry Science
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    • v.26 no.3
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    • pp.149-170
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    • 1999
  • Protozoa of the genus Eimeria are the etiologic agents of avian coccidiosis, the most economically important Parasitic disease for the poultry industry. Coccidia multiply in intestinal epithelial cells of a wide range of hosts, including livestock in addition to poultry. Chemotherapy is extensively used to control coccidiosis. However, development of drug resistance by Eimeria parasites, the intensive cost and labor involved in the identification of new anticoccidial compounds and public awareness of drug residues in foods warrant alternative methods to prevent coccidiocic in the fast growing poultry industry. For these reasons, there is a great interest in developing vaccines against avian coccidiosis. Live Eimeria vaccines confer protective immunity, however a significant disadvantage of using these types of vaccines is their pathogenicity. Live parasites with attenuated pathogenicity also usually produce immunity but may revert back to a pathogenic form and may be contaminated with other pathogenic organisms. Killed Eimeria vaccines are safer but, unlike live attenuated vaccines, are not able to generate cytotoxic T lymphocyte responses. Recombinant vaccines are biochemically purified proteins produced by genetic engineering that consist of particular epitopes or metabolites of Eimeria. Unlike live attenuated organisms, recombinant vaccines do not possess as much risk and generally are able to induce both humoral and cell mediated immunity. DNA vaccines consist of genes encoding immunogenic proteins of pathogens that are directly administered into the host in a manner that the gene is expressed and the resulting protein generates a protective immune response. Although all of these different types of vaccines have been applied to coccidiosis, this disease continues to cause substantial morbidity and mortality in the poultry industry. Future development of an effective vaccine against coccidiosis will depend on further investigation of protective immunity to Eimeria infection and identification of important immundgenic parasite molecules.

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Four newly reported ophichthid leptocephali species revealed by mitochondrial 12S rDNA, with implications of their occurrence in Korea

  • Ji, Hwan Sung;Lee, Hae Won;Hong, Byung Kyu;Kim, Jin Koo
    • Animal cells and systems
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    • v.16 no.5
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    • pp.415-424
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    • 2012
  • Four species of ophichthid leptocephali were identified using 12S rDNA sequences, and their morphological descriptions were first provided based on six individuals (S1-S3,M1, and E1-E2) collected from the East Sea and the Korea Strait between September 2008 and October 2010. Mitochondrial 12S rDNA 859-861 base pairs of ophichthid leptocephali were compared with those of 16 ophichthids adult and 2 outgroups (Anguilla japonica and Conger myriaster). Leptocephali of S1 and E1 were very closely clustered with adult of Scolecenchelys borealis (D=0.002) and Echelus uropterus (D=0.000), respectively. However, leptocephali of S2-S3 andM1 were slightly far clustered with leptocephalus of S1 (D=0.006) and adult of Muraenichthys gymnopterus (0.034), respectively. We believe that S1 and E1 are S. borealis and E. uropterus, respectively, in which the former is unrecorded species in Korea. However, S2-S3 and M1 may be undescribed species belonging to genus Scolecenchelys and Muraenichthys, respectively, because total numbers of myomeres for S2-S3 (148-158) and M1 (151) were not consistent with total numbers of vertebrae or distribution for any adult of Scolecenchelys spp. and Muraenichthys spp. in the world. We propose the new Korean name 'Dong-hae-mul-baem' for S. borealis.

Mass Mortality Caused by Nocardial Infection in Cultured Snakehead, Channa arga in Korea (Norcardia 감염증에 의한 양식 가물치의 대량 폐사)

  • Park, Myoung-Ae;Lee, Deok-Chan;Cho, Mi-Young;Choi, Hee-Jung;Kim, Jin-Woo
    • Journal of fish pathology
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    • v.18 no.2
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    • pp.157-165
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    • 2005
  • A new disease causing mass mortality of farmed snakehead (Channa arga) has emerged in Korea over the summer of 2005. The affected fish showed no specific external signs with the exception of a distended abdomen and hemorrhaging around the anus. After opening the abdomen, the visceral organs, liver, spleen and kidney, present numerous white nodular structures. Histopathological examination revealed these nodules to be evidence of granulomas in the visceral organs. A Gram-positive, filamentous bacterium was isolated from all of the affected fish. Development of primers for a genus-specific peR assay for Nocardia, following analysis of the sequences of the complete 16S rRNA genes from Nocardia spp. and non-Nocardia bacterial genes, allowed identification of the causative organism as Nocardia. This is the first report of a nocardial infection of fish in Korea.

Development of an Indirect Non-Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Cronobacter muytjensii in Infant Formula Powder (유아용 조제분유 내 Cronobacter muytjensii 검지를 위한 간접 비경합 면역분석법의 개발)

  • Song, Xinjie;Kim, Myunghee
    • The Korean Journal of Food And Nutrition
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    • v.26 no.4
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    • pp.936-944
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    • 2013
  • Cronobacter muytjensii is an important foodborne pathogen as a potential risk in infant formula powder (IFP). To develop a new and sensitive method for the detection of Cronobacter spp. in IFP, an immunoglobulin G (IgG) specific for C. muytjensii (formerly known as Enterobacter sakazakii ATCC 51329) was developed. Further, an indirect noncompetitive enzyme-linked immunosorbent assay (INC-ELISA) was developed by using the anti-C. muytjensii IgG. As a result, this newly developed INC-ELISA method was found very sensitive for C. muytjensii with detection limit of $6.5{\times}10^3CFU/ml$ in pure culture and 1 cell/25 g of IFP. This INC-ELISA method also displayed excellent specificity for C. muytjensii showing no cross-reactivity with other strains of Cronobacter genus and 11 other foodborne pathogenic strains. These results show that the developed INC-ELISA method was very sensitive, efficient, and rapid for the detection of C. muytjensii. Hence, this method could be applied to the development of diagnostic kits for the rapid and easy detection of C. muytjensii.