• 한국수소및신에너지학회논문집
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• 제4권1호
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• pp.51-64
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• 1993

• 한국수소및신에너지학회논문집
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• 제1권1호
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• pp.66-72
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• 1989

• 한국수소및신에너지학회논문집
• /
• 제1권1호
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• pp.58-65
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• 1989

• 한국수소및신에너지학회논문집
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• 제1권1호
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• pp.55-57
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• 1989

• 한국수소및신에너지학회논문집
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• 제12권2호
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• pp.87-102
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• 2001

• 신재생에너지
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• 제2권1호
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• pp.3-13
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• 2006

• 신재생에너지
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• 제2권1호
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• pp.26-32
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• 2006

• 신재생에너지
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• 제2권1호
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• pp.33-45
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• 2006

• Journal of Microbiology and Biotechnology
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• 제26권9호
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• pp.1570-1578
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• 2016

Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. gallica TCK was treated with N-methyl-N-nitro-N-nitrosoguanidine and ultraviolet light. Among the mutants isolated, T906 was found to be a high-production strain of laccases. The mutant strain T906 was stabilized via dozens of passages, and the selected ones were further processed for optimization of metallic ion, inducers, and nutritional requirements, which resulted in the optimized liquid fermentation medium MF9. The incubation temperature and pH were optimized to be 30℃ and 4.5, respectively. The mutant strain T906 showed 3-times higher laccase activity than the original strain TCK under optimized conditions, and the maximum laccase production (303 U/ml) was accomplished after 13 days. The extracellular laccase isoenzyme 1 was purified and characterized from the two strains, respectively, and their cDNA sequence was determined. Of note, the laccase isoenzyme 1 transcription levels were overtly increased in T906 mycelia compared with values obtained for strain TCK. These findings provide a basis for C. gallica modification for the production of high laccase amounts.

• 상하수도학회지
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• 제24권6호
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• pp.683-690
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• 2010

Renewable and unutilized energy (biogas power generation, wind power, solar, small hydro-power, sewage heat source, etc.) seems to be suitable to install for the sewage treatment facilities. There are 357 sewage treatment plants in 2007. 17 plants among these have been operating for self-supporting energy by using solar power, small hydro-power and biogas in 2008. Newly built sewage treatment plant of 96,000 $m^3$/day for a newtown is expected to get up to energy consumption of 10 GWh/yr. If solar energy, small hydro-power and biogas-equipments were applied to the new treatment plant, self-supporting energy of the new sewage treatment plant will get up to 56.1%. As a results, about 2,379ton $CO_2$/yr $CO_2$ emission reduction can be expected by using renewable energy. These efforts for self-supporting energy will lead sewage treatment plant to new energy recycle center.