• 대한불안의학회지
• /
• 제10권1호
• /
• pp.3-10
• /
• 2014

Reactive disorder is a group of diagnosis with a definitely known etiology and whose etiological factor is essential to the diagnosis. In DSM system, such reactive disorders are listed as adjustment disorder, acute stress disorder, brief psychotic disorder with marked stressor and posttraumatic stress disorder (PTSD). However, a growing number of individuals is suffering from a prolonged feeling of embitterment after exceptional negative life events and this condition could be diagnosed neither PTSD nor adjustment disorder nor depressive disorder in the context of DSM-IV diagnostic system. This clinical condition can be described as 'posttraumatic embitterment disorder' (PTED). PTED is a reactive disorder triggered by exceptional, though normal negative life events such as conflict in the workplace, unemployment, death of a relative, divorce, severe illness, or experience of loss or separation. The common feature of such events is that they are experienced as unjust, as a personal insult, accompanied by psychological violation of basic beliefs and values. The central psychopathological response pattern in PTED is a prolonged feeling of embitterment. In particular, the core emotion of embitterment can lead to the rejection of treatment. Therefore, "wisdom therapy" as a new treatment approach specifically designed for PTED has been developed. It is assumed that many patients suffering from PTED are often misunderstood and misdiagnosed. This review would help to introduce PTED into the clinical field in psychiatry.

• Molecules and Cells
• /
• 제38권11호
• /
• pp.925-935
• /
• 2015

DNA methylation is a well-characterized epigenetic modification that plays central roles in mammalian development, genomic imprinting, X-chromosome inactivation and silencing of retrotransposon elements. Aberrant DNA methylation pattern is a characteristic feature of cancers and associated with abnormal expression of oncogenes, tumor suppressor genes or repair genes. Ten-eleven-translocation (TET) proteins are recently characterized dioxygenases that catalyze progressive oxidation of 5-methylcytosine to produce 5-hydroxymethylcytosine and further oxidized derivatives. These oxidized methylcytosines not only potentiate DNA demethylation but also behave as independent epigenetic modifications per se. The expression or activity of TET proteins and DNA hydroxymethylation are highly dysregulated in a wide range of cancers including hematologic and non-hematologic malignancies, and accumulating evidence points TET proteins as a novel tumor suppressor in cancers. Here we review DNA demethylation-dependent and -independent functions of TET proteins. We also describe diverse TET loss-of-function mutations that are recurrently found in myeloid and lymphoid malignancies and their potential roles in hematopoietic transformation. We discuss consequences of the deficiency of individual Tet genes and potential compensation between different Tet members in mice. Possible mechanisms underlying facilitated oncogenic transformation of TET-deficient hematopoietic cells are also described. Lastly, we address non-mutational mechanisms that lead to suppression or inactivation of TET proteins in cancers. Strategies to restore normal 5mC oxidation status in cancers by targeting TET proteins may provide new avenues to expedite the development of promising anti-cancer agents.

• BMB Reports
• /
• 제52권12호
• /
• pp.712-717
• /
• 2019

Translocase of outer mitochondrial membrane 20 (TOMM20) plays an essential role as a receptor for proteins targeted to mitochondria. TOMM20 was shown to be overexpressed in various cancers. However, the oncological function and therapeutic potential for TOMM20 in cancer remains largely unexplored. The purpose of this study was to elucidate the underlying molecular mechanism of TOMM20's contribution to tumorigenesis and to explore the possibility of its therapeutic potential using colorectal cancer as a model. The results show that TOMM20 overexpression resulted in an increase in cell proliferation, migration, and invasion of colorectal cancer (CRC) cells, while siRNA-mediated inhibition of TOMM20 resulted in significant decreases in cell proliferation, migration, and invasion. TOMM20 expression directly impacted the mitochondrial function including ATP production and maintenance of membrane potential, which contributed to tumorigenic cellular activities including regulation of S phase cell cycle and apoptosis. TOMM20 was overexpressed in CRC compared to the normal tissues and increased expression of TOMM20 to be associated with malignant characteristics including a higher number of lymph nodes and perineural invasion in CRC. Notably, knockdown of TOMM20 in the xenograft mouse model resulted in a significant reduction of tumor growth. This is the first report demonstrating a relationship between TOMM20 and tumorigenesis in colorectal cancer and providing promising evidence for the potential for TOMM20 to serve as a new therapeutic target of colorectal cancer.

• Molecules and Cells
• /
• 제40권3호
• /
• pp.186-194
• /
• 2017

A brain-enriched secreting signal peptide, NELL2, has been suggested to play multiple roles in the development, survival, and activity of neurons in mammal. We investigated here a possible involvement of central NELL2 in regulating feeding behavior and metabolism. In situ hybridization and an immunohistochemical approach were used to determine expression of NELL2 as well as its colocalization with proopiomelanocortin (POMC) and neuropeptide Y (NPY) in the rat hypothalamus. To investigate the effect of NELL2 on feeding behavior, 2 nmole of antisense NELL2 oligodeoxynucleotide was administered into the lateral ventricle of adult male rat brains for 6 consecutive days, and changes in daily body weight, food, and water intake were monitored. Metabolic state-dependent NELL2 expression in the hypothalamus was tested in vivo using a fasting model. NELL2 was noticeably expressed in the hypothalamic nuclei controlling feeding behavior. Furthermore, all arcuatic POMC and NPY positive neurons produced NELL2. The NELL2 gene expression in the hypothalamus was up-regulated by fasting. However, NELL2 did not affect POMC and NPY gene expression in the hypothalamus. A blockade of NELL2 production in the hypothalamus led to a reduction in daily food intake, followed by a loss in body weight without a change in daily water intake in normal diet condition. NELL2 did not affect short-term hunger dependent appetite behavior. Our data suggests that hypothalamic NELL2 is associated with appetite behavior, and thus central NELL2 could be a new therapeutic target for obesity.

• 한국식품과학회지
• /
• 제34권3호
• /
• pp.536-539
• /
• 2002

본 연구에서는 증자 콩에 증식하여 부패취를 유발하는 오염균인 야생고초균의 생육을 억제하고 정상발효를 유도하기위해 젖산, 과산화수소 등과 항균성 물질인 bacteriocin을 생성하는 유산균으로 1단 발효를 실시한 후 장류용 황국균을 접종하여 2단 발효 후 다시 고초균으로 3단 발효시켜 황국균과 고초균의 protease, peptidase를 효과적으로 사용함으로서 단백질 분해율이 높아 아미노산 생성량이 많은 기호성 높은 고품질의 메주를 제조하였다. 3단 발효법으로 제조된 메주의 수분함량과 pH를 측정한 결과 각각 54.0 %와 pH 7.0였고 제조된 메주를 30일간 20% 염수로 용출하여 총 유리 아미노산함량을 비교한 결과 3단 발효법으로 제조된 메주구가 대조구에 비해 약 $3{\sim}5$배 높게 나타났다. 특히 장류의 맛을 좌우하는 감칠 맛 성분인 glutamic acid와 aspartic acid의 함량은 대조구에 비해 매우 높아 메주를 원료로 제조하는 장류의 품질을 향상시킬 수 있으며 유리아미노산이 용출되는 시간이 대조구보다 짧아 장류 제조시 발효기간을 1/3정도로 단축시킬 수 있다.

• 동아시아식생활학회지
• /
• 제17권3호
• /
• pp.289-303
• /
• 2007

This report includes basic data on nutrition education for Chinese adolescents living in Korea. A total of 183 Chinese adolescents living in Incheon participated in the survey. Body composition was analyzed by the Inbody 4.0., and questionnaires on personal dietary behavior were administrated. When compared with the body measure indices of Korean adolescents, we found that height was similar, but weight greater ($5{\sim}12 kg$) among the participants. Regarding obesity, 30.0% of all students were obese (BMI>25), especially the high school boys (42.0%). But in the case of middle school boys, the rates of obesity and under weight (BMI<18.5) were the same (both of them at 22.9%). The average WHR (Waist Hip Ratio) of the female high school group (0.9) was over the normal range. The average percent body fat for each group was also over the normal range. The percentage of students having breakfast everyday was 52.2%, and the rate of skipping breakfast was 25.0%. In the obesity group, the rate of skipping breakfast was remarkably high (P<0.05). Because lunch service was not available at the schools, a lunch box from home was the most popular pattern (43.1 %), but eating out was also popular at 36.6%. For the male high school students, the eating out rate was 62.8%. Since many have-lived in Korea for a few generations, the students' eating practices were much closer to the Korean pattern. For their ordinary meal pattern, 96.0% of the students included Korean foods. however, for New Years Day, the rate of having Gyo-Ja was 75.2%, and having Wol-Byung at Choo-seok was 78.6%, showing that the Chinese tradition still remains. In the case of food preferences, the percentage having Kimchi everyday was 62.1 %, but those drinking green tea was 13.1 %. For cold drinks, the rate for drinking them more than twice per week was 71.6%. The most preferred cooking method was frying with oil (50.6%). Among the students who had visited China (or Taiwan), the rate for 'bad food familiarity' was 37.0%, and the rate for 'did not know Chinese traditional course menus' was 71.3%. As a result of combining eating practices from both China and Korea, and the absence of lunch service in Chinese schools, students are showing bad dietary behaviors. therefore, it is necessary to provide proper nutrition education and a system for lunch service in Chinese schools.

• 한국농림기상학회지
• /
• 제18권4호
• /
• pp.298-306
• /
• 2016

주산지 배추재배농가에서 기후변화 적응수단의 하나로 사용할 수 있는 것이 정식기 이동이다. 본 연구에서는 여름배추 품종을 대상으로 주어진 정식일부터 매일 기온의 경과에 의해 최적수확기를 예측하고, 결정된 생육 기간 중 기온자료에 의해 배추의 잠재수량(생체중)을 추정할 수 있는 방법을 고안하였다. 이를 위해 정식기 이동에 따른 생육기간 중 기후조건 변화를 온도 기반 열단위로 표현하고, 이를 생육기와 결구기에 맞게 조절한 발육 속도함수에 적용하여 생리적 성숙기를 추정하는 생물계절모형을 개발하였다. 다음에는 생물계절모형에 의해 결정된 재배가능기간에 대하여 매일 열단위 누적에 의해 여름배추의 잠재수량을 계산할 수 있는 수량예측모형(Ahn et al., 2014)을 결합하였다. 이 생물계절-수량 결합모형을 RCP8.5 기반의 남한 상세 기후시나리오(2000-2100)에 적용하여 7월 1일, 8월 1일, 9월 1일, 그리고 10월 1일 등 다양한 날짜에 배추를 정식할 경우 현재평년(2001-2010)과 미래평년(2011-2040, 2041-2070, 2071-2100)에 예상되는 수량성을 잠재수량에 대한 백분율로 표현하였다. 그 결과를 토대로 남한 전역을 810개 집수역으로 나누고 임의 집수역의 최적정식일을 사용자가 손쉽게 찾을 수 있는 시간 - 공간 - 수량 3차원 평가도표를 고안하였다. 이 방법은 미래 새로운 재배적지 탐색은 물론 기존 주산지에서 품종변경 없이 기후변화 적응이 가능한 작부체계 개발에도 유용할 것으로 기대된다.

• 원예과학기술지
• /
• 제33권3호
• /
• pp.396-402
• /
• 2015

This study identified a new male-sterile germplasm of Chinese jujube, named male-sterile No. 2 (JMS2), and achieved controlled hybridization using that germplasm as the female parent. The anthers of JMS2 before flower bud opening became shrunken, dingy yellow and much smaller than normal ones, and they changed to brown after anthesis. No pollen was observed in anthers of JMS2 and its male-sterile trait remained stable over different years. A total of 1,642 fruits were obtained from ten intra- and interspecific cross combinations via controlled hybridization from 2008 to 2012 using JMS2 as the female parent. Of those, 27.3% produced seeds, on average (0-72.6%). The rate of fruit with seed (RFS) was significantly different between cross combinations or years. Compared to other cross combinations, the RFS in the combination of JMS2 ${\times}$ 'Xingguang' (a Chinese jujube cultivar with high resistance to jujube witches' broom disease) and JMS2 ${\times}$ 'Xing16' (a sour jujube genotype) remained high in different years and reached means of 48.7 and 58.1%, respectively. Finally, 150 plantlets were regenerated from immature embryos, and 51 of them were randomly selected and identified to be authentic hybrids using amplified fragment length polymorphism (AFLP) markers. This is the first report of hybrids obtained from a cross between Chinese jujube and sour jujube.

• 한국위성정보통신학회논문지
• /
• 제8권4호
• /
• pp.142-149
• /
• 2013

인공위성을 포함한 우주물체가 지구궤도로 재진입하고 대기권에서 소실되거나, 지표면에 추락하는 일들이 날로 증가하고 있다. 최근 국내에서도 위성추락상황실을 운영하여 인공위성의 추락고도 및 추락시기에 대한 예측을 실시하였다. 아직까지 국내에서는 우주물체의 추락 시 현업에 적용할 수 있는, 미전략사령부에서 발표하는 TLE 자료를 활용한 독자적인 추락예측모델이 없으며, 다른 위성 운영국에서 발표하는 추락 예상시기를 참고하였다. 이 연구에서는 TLE 자료에 대기모델을 바탕으로 추락예상시기를 예측하고, 분석한 결과를 종합 분석하였고, 위성추락상황실 운영간 적용했던 RUBBER SHEET SHIFT METHOD(RSSM)의 인공위성 예상추락시기 예측결과를 토대로 새로운 추락모델에 대한 예시를 제안하였다.

• Journal of Microbiology and Biotechnology
• /
• 제19권9호
• /
• pp.1070-1076
• /
• 2009

Telomerase reverse transcriptase (TERT), which prolongs the replicative life span of cells, is highly upregulated in 85-90% of human cancers, whereas most normal somatic tissues in humans express limited levels of the telomerase activity. Therefore, TERT has been a potential target for anticancer therapy. Recently, we described a new approach to human cancer gene therapy, which is based on the group I intron of Tetrahymena thermophila. This ribozyme can specifically mediate RNA replacement of human TERT (hTERT) transcript with a new transcript harboring anticancer activity through a trans-splicing reaction, resulting in selective regression of hTERT-positive cancer cells. However, to validate the therapeutic potential of the ribozyme in animal models, ribozymes targeting inherent transcripts of the animal should be developed. In this study, we developed a Tetrahymena-based trans-splicing ribozyme that can specifically target and replace the mouse TERT (mTERT) RNA. This ribozyme can trigger transgene activity not only also in mTERT-expressing cells but hTERT-positive cancer cells. Importantly, the ribozyme could selectively induce activity of the suicide gene, a herpes simplex virus thymidine kinase gene, in cancer cells expressing the TERT RNA and thereby specifically hamper the survival of these cells when treated with ganciclovir. The mTERT-targeting ribozyme will be useful for evaluation of the RNA replacement approach as a cancer gene therapeutic tool in the mouse model with syngeneic tumors.