• 대한화학회지
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• 제37권8호
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• pp.773-778
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• 1993

Tl$^+$이온 센서로서 크라운 에테르 B15C5와 DB18C6를 중성운반체로 한 PVC 액체막 이온 선택성 전극을 제작하였다. 막용매로는 DOA, NPPE 및 NPOE를 사용하였으며 친유성 염, KTClPB의 농도를 변화시킨 여러가지 조성의 막을 시험하였다. B15C5와 DB18C6 막 전극의 감응전위는 농도범위, 10$^{-1}$∼10$^{-5}$M에서 직선으로 나타났으며 최대 기울기는 전극에 따라서 40∼55 mV/decade였다. 선택계수는 분리용액법으로 결정하였으며 알카리금속 이온, 알칼리토금속 이온 및 일부 전이금속 이온에 대하여 좋은 선택성을 나타냈다. 제작된 액체막 전극은 Ph > 3 에서 안정한 감응전위를 보였다.

• Animal Bioscience
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• 제34권3_spc호
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• pp.463-470
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• 2021

Objective: This experiment was conducted to find out the immunological effects of wheat phytase when long-chain inorganic polyphosphate (polyP) treated with wheat phytase was added to a macrophage cell line, Raw 264.7, when compared to intact long-chain polyP. Methods: Nitric oxide (NO) production of Raw 264.7 cells exposed to P700, a long-chain polyP with an average of 1,150 phosphate residues, treated with or without wheat phytase, was measured by Griess method. Phagocytosis assay of P700 treated with or without phytase in Raw 264.7 cells was investigated using neutral red uptake. The secretion of tumor necrosis factor α (TNF-α) by Raw 264.7 cells with wheat phytase-treated P700 compared to intact P700 was observed by using Mouse TNF-α enzyme-linked immunosorbent assay kit. Results: P700 treated with wheat phytase effectively increased NO production of Raw 264.7 cells by 172% when compared with intact P700 at 12 h exposure. At 5 mM of P700 concentration, wheat phytase promoted NO production of macrophages most strongly. P700, treated with wheat phytase, stimulated phagocytosis in macrophages at 12 h exposure by about 1.7-fold compared to intact P700. In addition, P700 treated with wheat phytase effectively increased in vitro phagocytic activity of Raw 264.7 cells at a concentration above 5 mM when compared to intact P700. P700 dephosphorylated by wheat phytase increased the release of TNF-α from Raw 264.7 cells by 143% over that from intact P700 after 6 h exposure. At the concentration of 50 μM P700, wheat phytase increased the secretion of cytokine, TNF-α, by 124% over that from intact P700. Conclusion: In animal husbandry, wheat phytase can mitigate the long-chain polyP causing damage by improving the immune capabilities of macrophages in the host. Thus, wheat phytase has potential as an immunological modulator and future feed additive for regulating immune responses caused by inflammation induced by long-chain polyP from bacterial infection.

• 한국초지조사료학회지
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• 제43권3호
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• pp.168-176
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• 2023

Silicon (Si) has the potential to improve plant growth and stress tolerance. The study aimed to explore Si-involving plant responses and molecular characterization of different Si-responsive genes in alfalfa. In this study, the exogenous supplementation of Si enhanced plant growth, and biomass yield. Si-acquisition in alfalfa root and shoot was higher in Si-supplemented compared to silicon deficient (-Si) plants, implying Si-acquisition has beneficial on alfalfa plants. As a consequence, the quantum efficiency of photosystem II (Fv/Fm) was significantly increased in silicon-sufficient (+Si) plants. The quantitative gene expression analysis exhibited a significant upregulation of the Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes in alfalfa roots, while BOR1, BOR4, NIP2, and NIP3 showed no significant variation in their expression. The MEME results further noticed the association of four motifs related to the major intrinsic protein (MIP). The interaction analysis revealed that NIP5;1 and Lsi1 showed a shared gene network with NIP2, BOR1, and BOR4, and Lsi2, Lsi3 and NIP3-1, respectively. These results suggest that members of the major intrinsic proteins (MIPs) family especially Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes helped to pass water and other neutral solutes through the cell membrane and those played significant roles in Si uptake and transport in plants. Together, these insights might be useful for alfalfa breeding and genome editing approaches for alfalfa improvement.

• 한국환경과학회지
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• 제33권1호
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• pp.9-16
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• 2024

This study analyzed a survey of residents' awareness of rivers and water spaces that reflected residents' awareness in order to establish a basic park green space plan. Through this, the perception and requirements of rivers and water spaces were analyzed and important information was provided for urban planning and environmental management. As a result of analyzing residents' neutral satisfaction with rivers and their opinions on the frequency of use, role, and purpose, it was confirmed that rivers are recognized as an important part of urban life and contain the potential for development for a better urban environment. . However, considering the dissatisfied response and the relatively low frequency of use, we came to the conclusion that better urban planning and management is needed to meet the needs of residents by improving the river environment and providing various facilities. Based on this, city managers should focus on river management and improvement by actively collecting residents' opinions. Through analysis of the causes of dissatisfied responses, measures such as improving the environment around rivers and waste management should be prepared, and a broad strategy for environmental conservation and utilization should be established by reflecting the diverse expectations and demands of residents. These ongoing efforts are expected to have a positive impact on the sustainability of the city and improvement of residents' quality of life. Additionally, the results of this study will be able to contribute to establishing policies for urban planning and environmental improvement through similar research and analysis in other regions.

• 한국건설순환자원학회논문집
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• 제6권2호
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• pp.100-107
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• 2018

초고강도 섬유보강 콘크리트 50M 합성 박스거더에 대한 재료적 비선형 및 기하학적 비선형 유한요소해석이 수행되었다. 인장과 압축구역에서 구성방정식을 실험에 근거하여 모델링하였다. 비선형 유한요소해석의 정확성은 UHPFRC 50M 합성거더의 실험 결과와 비교하여 검증하였다. 1.5% 체적대비 섬유혼입률, 135MPa 압축강도 및 18MPa 휨인장강도 특성을 가진 UHPFRC 50M 합성거더에 대한 휨실험이 수행되었다. 포스트텐션힘으로 결합된 UHPFRC 합성거더는 3개의 UHPFRC 분절 U거더와 고강도 철근콘크리트 슬래브로 구성되었다. Midas FEA를 사용하여 UHPFRC 거더 부분은 8개 절점을 가진 3차원 6면체 모델링을 하였고, 철근와 강연선은 2개 절점을 가진 선형 요소로 모델링하였다. Total strain crack 모델에 기반을 둔 압축 및 인장 다중 선형모델을 사용하여 구성방정식을 설정하였고 균열은 smeared crack model로 구성하였다. 철근과 강연선의 비선형성은 Von Mises 규준을 적용하였다. 비선형 정적해석은 Newton-Rhapson 기법의 수렴치를 사용한 점진적 반복기법을 사용하여 해를 수행하였다. 유한요소해석은 하중-변위관계, 중립축 변화관계 및 균열양상에 대하여 실험 결과와 수치 해석 결과를 비교하여 검증하였다. 하중-변위 관계는 실험 결과와 비교해볼 때 매우 정확한 결과를 보여주고 있다. 본 논문에서 수행한 비선형 유한요소해석법은 철근보강 포스트텐션닝 초고강도 섬유보강 합성 박스거더의 휨거동 해석에 만족한 결과를 보여주고 있다.

• 한국콘텐츠학회논문지
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• 제14권5호
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• pp.224-234
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• 2014

본 연구의 목적은 임상실습 전 간호대학생의 전문 간호사의 이미지에 관한 주관성 유형을 파악하고자 Q방법론을 적용하였다. 연구대상자는 간호대학생 43명을 대상으로 전문 간호사 유형에 관한 34개의 진술문을 추출하였다. 진술문은 자신의 의견에 따라 +4점(가장 긍정하는 진술문)에서부터 0(중립) -4(가장 부정하는 진술문)을 선택하여 9점 척도상에 강제 분포하도록 하였다. 자료 분석은 PC QUANL program을 이용하였으며, 분석결과 eignvalue 1.0 이상을 가진 값을 가지고 3가지 유형이 나타났으며 변량은 53.94%(각각 41.63%, 7.41% and 4.9%)이었다. 전문 간호사에 대한 임상실습 전 간호대학생들의 인식 유형을 분석한 결과, 각기 독특한 특성을 가진 3개의 유형이 나타났다. 제1유형은 '나이팅게일 사상 중시형', 제2유형은 '환자 중시형', 제3유형은 '자기개발 중시형'으로 나타났다. 12명의 학생이 제1유형인 '나이팅게일 사상 중시형', 11명의 학생이 제2유형인 '환자 중시형', 2명의 학생이 제3유형인 '자기개발 중시형'에 속해 있었다. 본 연구 결과 전문 간호사에 대한 간호대학생들의 주관적인 구조에 차이점이 발견되었다. 이 연구 결과는 효과적인 간호 교육과 임상 실무에 활용될 수 있을 것이다.

• 감성과학
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• 제21권3호
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• pp.49-60
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• 2018

특성 분노는 분노 경험과 관련된 개인의 심리 특성 중 하나로, 특성 분노가 높은 이들은 낮은 이들에 비해 더 자주, 더 강하게, 더 길게 분노를 경험한다. 본 연구의 목적은 분노를 경험하는 동안에 활성화되는 뇌 영역을 관찰하고, 활성화된 뇌 영역 중 특성 분노와 연관이 있는 영역을 확인하는 것이다. 본 연구에서는 실험 참가자 35명을 대상으로 과거에 분노했던 기억을 회상하여 분노를 유발하는 과제를 수행하는 동안의 뇌 기능을 측정하였다. 정서적 중립 사건을 회상하는 조건에 비해 분노 사건을 회상하는 조건에서 더 활성화된 뇌 영역은 우측 superior temporal gyrus (BA38), 좌측 inferior frontal gyrus (BA45), 우측 thalamus, 우측 insula (BA13), 양측 cuneus (BA17), 그리고 우측 cerebellum anterior lobe으로 나타났다. 분노 회상 시 뇌 활성화 정도와 특성 분노 점수 간 상관 분석 결과, 특성 분노 점수가 높을수록 분노 회상시 우측 insula의 활성화가 높았다. Insula가 정서 경험과 신체 내부감각수용기에서 오는 정보를 통합하여 자극에 대한 주관적인 느낌을 표상하는 영역으로 알려져 있으므로, 특성 분노가 높은 사람일수록 분노 자극에 대한 주관적인 느낌을 더 강하게 표상한다고 해석할 수 있다. 이 연구 결과는 분노의 개인차에 대한 뇌 기제를 규명한 것으로, 분노 및 분노 조절 관련 문제들에 대한 신경과학적 이해를 향상시키는데 기여할 것으로 기대된다.

• 농촌의학ㆍ지역보건
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• 제21권2호
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• pp.221-230
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• 1996

This study was purposed to develop the fluoride mouthrinsing project through the public health system in a rural area. This project was performed under the management of the public health dentist for the 3rd grade students selected from the 5 primary schools at Goksunggun in Chonnam area. Fluoride mouthrinsing was executed with 0.2% neutral sodium fluoride solution once a week. After 1 year's fluoride mouthrinsing project, DMFT index, DMF rate frequencies of toothbrushing, simplified oral hygiene index, and the responses to fluoride mouthrinsing project of the school children participated were examined. The results obtained from this study were as follows: 1. The fluoride mouthrinsing project for school children was performed average 10 times a year by the public health dentist. They were relatively little active to perform this project. 2. There was no significant difference in the increasement of DMFT index and DMF rate during 1 year between control and fluoride mouthrinsing group. 3. Control group was increased higher than fluoride mouthrinsing group in simplified oral hygiene index. 4. Toothbrushing frequencies increased in both groups. 5. 80.4% of school children participated in fluoride mouthrinsing project was responded to like it, and 78.2% among them was responded the project to be beneficial for their dental health. 6. 39.2% of school children was responded to have water-like taste for fluoride solution. But 34.0% of them was done to have strange taste. On consideration of above findings, this fluoride mouthrinsing project was not well performed by the public health dentists and also the oral health status of school children participated was not significantly improved by this project. Therefore, to perform continually this project well, it was recommended that this project should be performed by other person such as dental hygienist rather than public health dentist.

• Asian-Australasian Journal of Animal Sciences
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• 제29권11호
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• pp.1585-1592
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• 2016

The present experiment was conducted to evaluate the effect of simulated heat stress on digestibility and methane ($CH_4$) emission. Four non-lactating crossbred cattle were exposed to $25^{\circ}C$, $30^{\circ}C$, $35^{\circ}C$, and $40^{\circ}C$ temperature with a relative humidity of 40% to 50% in a climatic chamber from 10:00 hours to 15:00 hours every day for 27 days. The physiological responses were recorded at 15:00 hours every day. The blood samples were collected at 15:00 hours on 1st, 6th, 11th, 16th, and 21st days and serum was collected for biochemical analysis. After 21 days, fecal and feed samples were collected continuously for six days for the estimation of digestibility. In the last 48 hours gas samples were collected continuously to estimate $CH_4$ emission. Heat stress in experimental animals at $35^{\circ}C$ and $40^{\circ}C$ was evident from an alteration (p<0.05) in rectal temperature, respiratory rate, pulse rate, water intake and serum thyroxin levels. The serum lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activity and protein, urea, creatinine and triglyceride concentration changed (p<0.05), and body weight of the animals decreased (p<0.05) after temperature exposure at $40^{\circ}C$. The dry matter intake (DMI) was lower (p<0.05) at $40^{\circ}C$ exposure. The dry matter and neutral detergent fibre digestibilities were higher (p<0.05) at $35^{\circ}C$ compared to $25^{\circ}C$ and $30^{\circ}C$ exposure whereas, organic matter (OM) and acid detergent fibre digestibilities were higher (p<0.05) at $35^{\circ}C$ than $40^{\circ}C$ thermal exposure. The $CH_4$ emission/kg DMI and organic matter intake (OMI) declined (p<0.05) with increase in exposure temperature and reached its lowest levels at $40^{\circ}C$. It can be concluded from the present study that the digestibility and $CH_4$ emission were affected by intensity of heat stress. Further studies are necessary with respect to ruminal microbial changes to justify the variation in the digestibility and $CH_4$ emission during differential heat stress.

• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.997-1002
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• 2005

The objective of this experiment was to determine agronomic characteristics, and forge yield and quality responses of rye variety to location and year for a double-cropping system in South Korea. Five forage rye varieties ('Koolgrazer', 'Jlee select', '9909', 'Homill22', 'Danko') were evaluated for forage quality and yields during the two growing years (the First year; 2001-2002, and the Second year; 2002-2003) at two locations (the central and southern zone). The heading date of rye in the central zone (Cheonan) was 15 April, and that of the southern zone (Daegu) was 10 April. The heading dates of 'Koolgrazer', 'Jlee select' and '9909' were earlier than those of the others. Although the five varieties had resistance to foliar diseases and insects, these had less lodging resistance. Dry matter (DM) content, DM and total digestible nutrients (TDN) yields had significant differences among rye varieties. DM and TDN yields of the rye varieties at the second year were higher than the first year in the central zone. In the southern zone, DM and TDN yields at the first year were lower than the second year. 'Jlee select' and '9909' had higher DM and TDN yields than the other varieties in both location and year except the first year of the central zone (p<0.001). The crude protein (CP) contents of rye grown in Cheonan were higher than Daegu. The CP content of 'Danko' variety was highest among tested varieties. The acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents were greater for the varieties grown in Cheonan and in the second year. The ADF and NDF contents of 'Danko' were lowest, while 'Danko' in TDN and relative feed value (RFV) was highest among rye varieties. Our study showed that there are differences in forage production and quality from location to location, year to year and variety to variety. Therefore, forage quality and forage production are an important factor for screening forage rye varieties. Early maturing varieties should be recommended as high-yielding forage in a double-cropping system because their production of forage was greatest early in the season.