Browse > Article
http://dx.doi.org/10.5713/ajas.20.0060

Potential immune-modulatory effects of wheat phytase on the performance of a mouse macrophage cell line, Raw 264.7, exposed to long-chain inorganic polyphosphate  

An, Jeongmin (Department of Animal Science and Technology, Konkuk University)
Cho, Jaiesoon (Department of Animal Science and Technology, Konkuk University)
Publication Information
Animal Bioscience / v.34, no.3_spc, 2021 , pp. 463-470 More about this Journal
Abstract
Objective: This experiment was conducted to find out the immunological effects of wheat phytase when long-chain inorganic polyphosphate (polyP) treated with wheat phytase was added to a macrophage cell line, Raw 264.7, when compared to intact long-chain polyP. Methods: Nitric oxide (NO) production of Raw 264.7 cells exposed to P700, a long-chain polyP with an average of 1,150 phosphate residues, treated with or without wheat phytase, was measured by Griess method. Phagocytosis assay of P700 treated with or without phytase in Raw 264.7 cells was investigated using neutral red uptake. The secretion of tumor necrosis factor α (TNF-α) by Raw 264.7 cells with wheat phytase-treated P700 compared to intact P700 was observed by using Mouse TNF-α enzyme-linked immunosorbent assay kit. Results: P700 treated with wheat phytase effectively increased NO production of Raw 264.7 cells by 172% when compared with intact P700 at 12 h exposure. At 5 mM of P700 concentration, wheat phytase promoted NO production of macrophages most strongly. P700, treated with wheat phytase, stimulated phagocytosis in macrophages at 12 h exposure by about 1.7-fold compared to intact P700. In addition, P700 treated with wheat phytase effectively increased in vitro phagocytic activity of Raw 264.7 cells at a concentration above 5 mM when compared to intact P700. P700 dephosphorylated by wheat phytase increased the release of TNF-α from Raw 264.7 cells by 143% over that from intact P700 after 6 h exposure. At the concentration of 50 μM P700, wheat phytase increased the secretion of cytokine, TNF-α, by 124% over that from intact P700. Conclusion: In animal husbandry, wheat phytase can mitigate the long-chain polyP causing damage by improving the immune capabilities of macrophages in the host. Thus, wheat phytase has potential as an immunological modulator and future feed additive for regulating immune responses caused by inflammation induced by long-chain polyP from bacterial infection.
Keywords
Animal Husbandry; Immunological Effects; Long-chain Inorganic Polyphosphate; Macrophage; Wheat Phytase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Dersjant-Li Y, Awati A, Schulze H, Partridge G. Phytase in non-ruminant animal nutrition: a critical review on phytase activities in the gastrointestinal tract and influencing factors. J Sci Food Agric 2015;95:878-96. https://doi.org/10.1002/jsfa.6998   DOI
2 Dionisio G, Holm PB, Brinch-Pedersen H. Wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) multiple inositol polyphosphate phosphatases (MINPPs) are phytases expressed during grain filling and germination. Plant Biotechnol J 2007; 5:325-38. https://doi.org/10.1111/j.1467-7652.2007.00244.x   DOI
3 Triantafillidis JK, Triantafyllidi A, Vagianos C, Papalois A. Favorable results from the use of herbal and plant products in inflammatory bowel disease: evidence from experimental animal studies. Ann Gastroenterol 2016;29:268-81. https://doi.org/10.20524/aog.2016.0059   DOI
4 Klimp AH, de Vries EGE, Scherphof GL, Daemen T. A potential role of macrophage activation in the treatment of cancer. Crit Rev Oncol Hematol 2002;44:143-61. https://doi.org/10.1016/S1040-8428(01)00203-7   DOI
5 Liu CF, Tseng KC, Chiang SS, Lee BH, Hsu WH, Pan TM. Immunomodulatory and antioxidant potential of Lactobacillus exopolysaccharides. J Sci Food Agric 2011;91:2284-91. https://doi.org/10.1002/jsfa.4456   DOI
6 Zhang W, Wang R, Giesy JP, Li Y, Wang P. Tris (1,3-dichloro-2-propyl) phosphate treatment induces DNA damage, cell cycle arrest and apoptosis in murine RAW264.7 macrophages. J Toxicol Sci 2019;44:133-44. https://doi.org/10.2131/jts.44.134   DOI
7 Kilaparty SP, Singh A, Baltosser WH, Ali N. Computational analysis reveals a successive adaptation of multiple inositol polyphosphate phosphatase 1 in higher organisms through evolution. Evol Bioinform 2014;10:239-50. https://doi.org/10.4137/EBO.S18948   DOI
8 An J, Cho J. Catalytic properties of wheat phytase that favorably degrades long-chain inorganic polyphosphate. Asian-Australas J Anim Sci 2020;33:127-31. https://doi.org/10.5713/ajas.19.0047   DOI
9 Brown MRW, Kornberg A. Inorganic polyphosphate in the origin and survival of species. Proc Natl Acad Sci USA 2004; 101:16085-7. https://doi.org/10.1073/pnas.0406909101   DOI
10 Corr EM, Cunningham CC, Helbert L, McCarthy GM, Dunne A. Osteoarthritis-associated basic calcium phosphate crystals activate membrane proximal kinases in human innate immune cells. Arthritis Res Ther 2017;19:23. https://doi.org/10.1186/s13075-017-1225-0   DOI
11 Morita H, He F, Fuse T, et al. Cytokine production by the murine macrophage cell line J774.1 after exposure to lactobacilli. Biosci Biotechnol Biochem 2002;66:1963-6. https://doi.org/10.1271/bbb.66.1963   DOI
12 Brown MRW, Kornberg A. The long and short of it - polyphosphate, PPK and bacterial survival. Trends Biochem Sci 2008; 33:284-90. https://doi.org/10.1016/j.tibs.2008.04.005   DOI
13 Pokhrel A, Lingo JC, Wolschendorf F, Gray MJ. Assaying for inorganic polyphosphate in bacteria. J Vis Exp 2019;143: e58818. https://doi.org/10.3791/58818   DOI
14 Yang J, Yang L, Tian L, Ji X, Yang L, Li L. Sphingosine 1-phosphate (S1P)/S1P receptor2/3 axis promotes inflammatory M1 polarization of bone marrow-derived monocyte/macrophage via G(α)i/o/PI3K/JNK pathway. Cell Physiol Biochem 2018; 49:1677-93. https://doi.org/10.1159/000493611   DOI
15 Delcenserie V, Martel D, Lamoureux M, Amiot J, Boutin Y, Roy D. Immunomodulatory effects of probiotics in the intestinal tract. Curr Issues Mol Biol 2008;10:37-54. http://doi.org/10.21775/cimb.010.037   DOI
16 Mizumachi K, Aoki R, Ohmori H, Saeki M, Kawashima T. Effect of fermented liquid diet prepared with Lactobacillus plantarum LQ80 on the immune response in weaning pigs. Animal 2009;3:670-6. https://doi.org/10.1017/S1751731109003978   DOI
17 Yang L, Sun Y, Liu Y, et al. PTP1B promotes macrophage activation by regulating the NF-κB pathway in alcoholic liver injury. Toxicol Lett 2020;319:11-21. https://doi.org/10.1016/j.toxlet.2019.11.001   DOI
18 Zhao J, Yan S, Zhu X, Bai W, Li J, Liang C. PTPRO exaggerates inflammation in ulcerative colitis through TLR4/NF-κB pathway. J Cell Biochem 2020;121:1061-71. https://doi.org/10.1002/jcb.29343   DOI
19 Liu N, Ru YJ, Cowieson AJ, Li FD, Cheng XCH. Effects of phytate and phytase on the performance and immune function of broilers fed nutritionally marginal diets. Poult Sci 2008;87:1105-11. https://doi.org/10.3382/ps.2007-00517   DOI
20 Kim KS, Rao NN, Fraley CD, Kornberg A. Inorganic polyphosphate is essential for long-term survival and virulence factors in Shigella and Salmonella spp. Proc Natl Acad Sci USA 2002;99:7675-80. https://doi.org/10.1073/pnas.112210499   DOI
21 Varas MA, Riquelme-Barrios S, Valenzuela C, et al. Inorganic polyphosphate is essential for Salmonella Typhimurium virulence and survival in Dictyostelium discoideum. Front Cell Infect Microbiol 2018;8:8. https://doi.org/10.3389/fcimb.2018.00008   DOI
22 Kumar A, Gangaiah D, Torrelles JB, Rajashekara G. Polyphosphate and associated enzymes as global regulators of stress response and virulence in Campylobacter jejuni. World J Gastroenterol 2016;22:7402-14. https://doi.org/10.3748/wjg.v22.i33.7402   DOI
23 Lorenz B, Schroder HC. Mammalian intestinal alkaline phosphatase acts as highly active exopolyphosphatase. Biochim Biophys Acta Protein Stuct Mol Enzymol 2001;1547:254-61. https://doi.org/10.1016/S0167-4838(01)00193-5   DOI
24 Dinarvand P, Hassanian SM, Qureshi SH, et al. Polyphosphate amplifies proinflammatory responses of nuclear proteins through interaction with receptor for advanced glycation end products and P2Y1 purinergic receptor. Blood 2014;123:935-45. https://doi.org/10.1182/blood-2013-09-529602   DOI
25 Hassanian SM, Avan A, Ardeshirylajimi A. Inorganic polyphosphate: a key modulator of inflammation. J Thromb Haemost 2017;15:213-8. https://doi.org/10.1111/jth.13580   DOI
26 Yan F, Polk DB. Probiotics and immune health. Curr Opin Gastroenterol 2011;27:496-501. https://doi.org/10.1097/MOG.0b013e32834baa4d   DOI
27 Hassanian SM, Dinarvand P, Smith SA, Rezaie AR. Inorganic polyphosphate elicits pro-inflammatory responses through activation of the mammalian target of rapamycin complexes 1 and 2 in vascular endothelial cells. J Thromb Haemost 2015; 13:860-71. https://doi.org/10.1111/jth.12899   DOI
28 Zhu J, Zhao L, Guo H, Jiang L, Ren F. Immunomodulatory effects of novel bifidobacterium and lactobacillus strains on murine macrophage cells. Afr J Microbiol Res 2011;5:8-15. https://doi.org/10.5897/AJMR10.589   DOI
29 Song G, Du Q. Isolation of a polysaccharide with anticancer activity from Auricularia polytricha using high-speed countercurrent chromatography with an aqueous two-phase system. J Chromatogr A 2010;1217:5930-4. https://doi.org/10.1016/j.chroma.2010.07.036   DOI