• IMMUNE NETWORK
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• v.9 no.6
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• pp.236-242
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• 2009

Background: Melanoma is the most fatal form of skin cancer due to its rapid metastasis. Recently, several studies reported that selenium can induce apoptosis in melanoma cells. However, the precise mechanism remains to be elucidated. In this study, we investigated the effect of selenium on cell proliferation in murine melanoma and on tumor growth and metastasis in C57BL/6 mice. Methods: Cell proliferation was measured by MTT assay in selenium-treated melanoma cells. Cell cycle distribution was analysized by staining DNA with propidum iodide (PI). mRNA and protein expression related to cell cycle arrest was measured by reverse transcription PCR and western blot. Tumor growth and metastasis was measured by in vivo model. Results: Selenium was suppressed the proliferation of melanoma cells in a dose dependent manner. The growth inhibition of melanoma by selenium was associated with an arrest of cell cycle distribution at G0/G1 stage. The mRNA and protein level of CDK2/CDK4 was suppressed by treatment with selenium in a time-dependent manner. In vivo, tumor growth was not suppressed by selenium; however tumor metastasis was suppressed by selenium in mouse model. Conclusion: These results suggest that selenium might be a potent agent to inhibit proliferative activity of melanoma cells.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.25 no.5
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• pp.1123-1129
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• 2015

Macro(automatic hunting) of mobile game is a program that touch the screen by defined rules like a game bot in PC online games, and it is used by make various ways like android application or windows application program. This gives honest users deprivation and make to lose their interest. Finally they would leave the game and gradually game life would be shorten. Although many studies to prevent these problems in PC online game are conducted, applying mobile game to PC's way is difficult because mobile games are limited to use the network and device performance is different with PC. In this paper, we propose a framework for macro detection by using the touch event information. A touch event on the mobile game is a necessary control command to the game. Because macro touches the screen with the same pattern, there is a difference between normal user's behavior and macro's operation. In mobile games that casual games are mostly, Touch event is the best difference that identify normal user against macro for a short period of time. As a result of detecting macros used in real mobile game by using the proposed framework it showed 100% accuracy and 0% false positive rate.

• Journal of KIISE:Computing Practices and Letters
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• v.16 no.4
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• pp.383-391
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• 2010

A Lifelog management system provides users with services to store, manage, and search their life logs. This paper proposes a fully-automatic collecting method of real world social contacts and lifelog search engine using collected social contact information as keyword. Wireless short-distance network devices in mobile phones are used to detect social contacts of their users. Human-Bluetooth relationship matrix is built based on the frequency of a human-being and a Bluetooth device being observed at the same time. Results show that with 20% of social contact information out of full social contact information of the observation times used for calculation, 90% of human-Bluetooth relationship can be correctly acquired. A lifelog search-engine that takes human names as keyword is suggested which compares two vectors, a row of Human-Bluetooth matrix and a vector of Bluetooth list scanned while a lifelog was created, using vector information retrieval model. This search engine returns more lifelog than existing text-matching search engine and ranks the result unlike existing search-engine.

• IMMUNE NETWORK
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• v.3 no.4
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• pp.302-309
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• 2003

Background: CTLA4 (CD152), which is expressed on the surface of T cells following activation, has a much higher affinity for B7 molecules comparing to CD28, and is a negative regulator of T cell activation. In contrast to stimulating and agonistic capabilities of monoclonal antibodies specific to CTLA-4, CTLA4Ig fusion protein appears to act as CD28 antagonist and inhibits in vitro and in vivo T cell priming in variety of immunological conditions. We've set out to confirm whether inhibition of the CD28-B7 costimulatory response using a soluble form of human CTLA4Ig fusion protein would lead to persistent inhibition of alloreactive T cell activation. Methods: We have used CHO-$dhfr^-$ cell-line to produce CTLA4Ig fusion protein. After serum free culture of transfected cell line we purified this recombinant molecule by using protein A column. To confirm characterization of fusion protein, we carried out a series of Western blot, SDS-PAGE and silver staining analyses. We have also investigated the efficacy of CTLA4Ig in vitro such as mixed lymphocyte reaction (MLR) & cytotoxic T lymphocyte (CTL) response and in vivo such as experimental autoimmune encephalomyelitis (EAE), graft versus host disease (GVHD) and skin-graft whether this fusion protein could inhibit alloreactive T cell activation and lead to immunosuppression of activated T cell. Results: In vitro assay, CTLA4Ig fusion protein inhibited immune response in T cell-specific manner: 1) Human CTLA4Ig inhibited allogeneic stimulation in murine MLR; 2) CTLA4Ig prevented the specific killing activity of CTL. In vivo assay, human CTLA4Ig revealed the capacities to induce alloantigen-specific hyporesponsiveness in mouse model: 1) GVHD was efficiently blocked by dose-dependent manner; 2) Clinical score of EAE was significantly decreased compared to nomal control; 3) The time of skin-graft rejection was not different between CTLA4Ig treated and control group. Conclusion: Human CTLA4Ig suppress the T cell-mediated immune response and efficiently inhibit the EAE, GVHD in mouse model. The mechanism of T cell suppression by human CTLA4Ig fusion protein may be originated from the suppression of activity of cytotoxic T cell. Human CTLA4Ig could not suppress the rejection in mouse skin-graft, this finding suggests that other mechanism except the suppression of cytotoxic T cell may exist on the suppression of graft rejection.

• 한국HCI학회:학술대회논문집
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• 2008.02b
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• pp.239-244
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• 2008

We lead a life in our community, beginning a new relationship with a stranger or maintaining or stopping existing relationships. Relationships with others are sustained through social activities based on communication. Generally speaking, by exchanging feelings and information through communication, relationships are formed and continued, and strengthened through active communication As a result of the development of technologies and information technology over the recent 10 years, a mobile phone has stood as a communication channel, and now it has become such a universal, and highly intimate and important means of communication that almost all the Koreans use more often than wired phones. Today, people have their communication channel available for others, by using a mobile phone at any time and anywhere. Like this, mobile phones have been playing a key role in helping people maintain, repair and strengthen their personal relationship, but from the perspective of personal relationship management, they still remain as an aid to help communication, failing to provide a positive help for actual relationship management. This study was designed to provide services supporting user's personal relationship management, focusing on the use of mobile phones as a major tool of communication, aiming to enable users to understand current state of their relationship and make relationship management efforts, or communication behaviors, by informing who needs communication, on the basis of data on mobile phone calls. To this end, the study established a method to extract intimacy between users and callers and develop a prototype of services supporting personal relationship management, using relationship characteristics in terms of mobile communication.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.7 no.3
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• pp.143-149
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• 2012

With the increasing use of mobile phones, mobile game is emerging as an entertainment for move. Furthermore, smart phone is widely spread, so mobile game market is rapidly growing. According to "Republic of Korea 2010 Games White Paper" mobile game market is growing annual average 10% or more, and is expected to reach $ 9.8 billion in 2011. It is almost doubled compared to 2007. It became possible to realize complicated high-capacity game with mobile phone developed to smart phone. And at the same time, mobile game is associated with Social Network Services (SNS) and is worldwide growing as a part of 'mobile life'. Apple launched an application open market, App Store, in 2008, and mobile game industry welcomed an innovative turning point. Global smart phone application market (App Store) is opened, so Gameday is able to launch mobile games easily to the global market getting out of the domestic market. In addition, Apple App Store has the structure of sharing their revenue with the developer with 7 to 3 which is more favorable terms for the developer compared to supply mobile contents to the mobile carrier who monopolized the market in the past. The largest proportion of the current App Store application is game, and Gameday has the opportunity to make a significant leap forward by keeping pace with the new environment changes.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.4
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• pp.1413-1424
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• 2013

Recently, the frequency of severe storms increases in Korea. Severe storms occurring in a short time cause huge losses of both life and property. A considerable research has been performed for the flood control system development based on an accurate stream discharge prediction. A physical model is mainly used for flood forecasting and warning. Physical rainfall-runoff models used for the conventional flood forecasting process require extensive information and data, and include uncertainties which can possibly accumulate errors during modelling processes. ANFIS, a data driven model combining neural network and fuzzy technique, can decrease the amount of physical data required for the construction of a conventional physical models and easily construct and evaluate a flood forecasting model by utilizing only rainfall and water level data. A data driven model, however, has a disadvantage that it does not provide the mathematical and physical correlations between input and output data of the model. The characteristics of a data driven model according to functional options and input data such as the change of clustering radius and training data length used in the ANFIS model were analyzed in this study. In addition, the applicability of ANFIS was evaluated through comparison with the results of HEC-HMS which is widely used for rainfall-runoff model in Korea. The neuro-fuzzy technique was applied to a Cheongmicheon Basin in the South Han River using the observed precipitation and stream level data from 2007 to 2011.

• Korean journal of communication and information
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• v.16
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• pp.36-70
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• 2001

The purpose of this study is to investigate women's radio talk program and evaluate its possibilities and limitation from the point of feminist perspective. The theoretical framework is based on feminist studies and text analysis of talk show. Existing studies regarding talk show are mainly focused on television. But radio talk show is one of general entertainment and it's influences on minorities are still significant. is the most representative women's radio talk program in Korea. It has been broadcasted over 10 years and very popular among Korean housewives. The audience of this program call themselves schoolfellow, and call the program 'school of women'. The media text of is mainly consisted of letters from women audiences, and they are selected by producers. So the text is made by both audiences and producers. The unique combination of this process produces complicated discourses which contain women's experiences in letters and station's considerations through safekeeping. The problems investigated in this study are as follows: First, What discourses are produced in this program? Second, Alternative possibilities can be seen in this program in feminist perspectives? Text analysis of 1week(2000.9.18-9.24) and interview with producers are accomplished to this purposes. In the text analysis, subject matters, inscribed women's position, values of the letters are revealed. Most of the subject matters are family affairs. Some are socially oriented but family and home are the predominant category of women's letters. And the position of women subject is defined in the domestic network. They are nameless but the mother, wife, daughter, daughter-in-law of other people. In value, family-oriented value and small happiness in everyday life are generally appeared. But these values are essentially coincide with the values of status quo. The answers of the conflict are not public but individualized. And acception the status quo is presented as the wisest decision, But ` has many implications in relation to women's sharing of their experience, and construction of imagined community in media. Women continuously interact each other revealing and discussing their experiences and sometimes their social practices are stirred through this media sphere. So we see the 'emotional union' among women are formed through radio. The limitation of this program is very apparent: it's patriarchic values, acception of status quo, and individualization of the women's problems. But in the same time we can read coexisting it's latent possibilities: the possibilities of women's public sphere. But it is completely alternative women's sphere in feminist perspectives. It renders women opportunities to participate public media and share with other women, and collaborate with their problem.

• Journal of Broadcast Engineering
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• v.22 no.3
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• pp.282-294
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• 2017

In this paper, we propose a novel intelligent headlight control (IHC) system which is durable to various road lights and camera movement caused by vehicle driving. For detecting candidate light blobs, the region of interest (ROI) is decided as front ROI (FROI) and back ROI (BROI) by considering the camera geometry based on perspective range estimation model. Then, light blobs such as headlights, taillights of vehicles, reflection light as well as the surrounding road lighting are segmented using two different adaptive thresholding. From the number of segmented blobs, taillights are first detected using the redness checking and random forest classifier based on Haar-like feature. For the headlight and taillight classification, we use the random forest instead of popular support vector machine or convolutional neural networks for supporting fast learning and testing in real-life applications. Pairing is performed by using the predefined geometric rules, such as vertical coordinate similarity and association check between blobs. The proposed algorithm was successfully applied to various driving sequences in night-time, and the results show that the performance of the proposed algorithms is better than that of recent related works.

• Animal cells and systems
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• v.4 no.2
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• pp.157-163
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• 2000

Yeast pheromone a-factor is a 13-amino acid peptide hormone that is synthesized as a part of a larger precursor, prepro-$\alpha$-factor, consisting of a signal peptide and a proregion of 64 amino acids. The carboxy-terminal half of the precursor contains four tandem copies of mature $\alpha$-factor. To investigate the molecular basis of intracellular sorting, proteolytic processing, and storage of the peptide hormone, yeast prepro-$\alpha$-factor precursors were heterologously expressed in rat pituitary $GH_3 cells. When cells harboring the precursor were metabolically labeled, a species of approximately 27 kD appeared inside the cells. Digestion with peptide: N-glycosidase F (PNG-F) shifted the molecular mass to a 19 kD, suggesting that the 27 kD protein was the glycosylated form as in yeast cells. The nascent polypeptide is efficiently targeted to the ER in the $GH_3 cells, where it undergoes cleavage of its signal peptide and core glycosylation to generate glycosylated pro-a-factor. To look at the post ER intracellular processing, the pulse-labelled cells were chased up to 2 hrs. The nascent propeptides disappeared from the cells at a half life of 30 min and only 10-25% of the newly synthesized, unprocessed precursors were stored intracellularly after the 2 h chase. However, about 20% of the pulse-labeled pro-$\alpha$-factor precursors were secreted into the medium in the pro-hormone form. With increasing chase time, the intracellular level of propeptide decreased, but the amount of secreted propeptide could not account for the disappearance of intracellular propeptide completely. This disappearance was insensitive to lysosomotropic agents, but was inhibited at $16^{circ}C or 20^{\circ}C$, suggesting that the turnover of the precursors was not occurring in the secretory pathway to trans Golgi network (TGN) or dependent on acidic compartments. From these results, it is concluded that a pan of these heterologous precursors may be processed at its paired dibasic sites by prohormone processing enzymes located in TGN/secretpry vesicles producing small peptides, and that the residual unprocessed precursors may be secreted into the medium rather than degraded intracellularly.