• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.6
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• pp.491-497
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• 2001

Polymethylmethacrylate(PMMA) is currently commonly used material for the reconstruction of bone defects and fixation of joint prosthetics following congenital and acquired causes. Although PMMA has widespread use, it does not possess the ideal mechanical characteristics with osteoconductivity and osteoinductivity required. In order to overcome these problem, addition of bovine bone drived defatting demineralized bone(BDB) powders to a PMMA bone cement was done for improvement of physical property and bone forming characteristics of composite. In order to investigate the influence of BDB reinforcement on the PMMA, we measured physical property of compressive, tensile, flexural strength, and scanning electron microscopic examinations. The results were obtained as follows: 1. The PMMA forms a solid cellular matrix with open cells about $100{\mu}m$ in variable size and incorporating BDB. BDB aggregates inside the cells form a porous network that is accessible from the outer surface. 2. The physical properties were compressive strength of mean $22.74{\pm}1.69MPa$, tensile strength of mean $22.74{\pm}1.69MPa$, flexural strength of mean $77.53{\pm}6.93MPa$. Scanning electron microscopic examinations were revealed that there was DBD particles form a highly porous agglomerates. BDB can be added PMMA in the form of dried powders, the composites are applicable as bone substitutes. BDB and PMMA mixture is shown to produce a class of composites that due to their microstructure and improved mechanical properties may be suitable for application as bone subsitutes. The mechanical and material properties of the BDB-PMMA bone substitute composites are competitive with those properties of a porous ceramic matrix of other hydroxyapatite and with those of natural bones.

• Journal of the Korean Association of Geographic Information Studies
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• v.10 no.3
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• pp.113-122
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• 2007

The rapid growth of aerial survey and remote sensing technology has enabled the rapid acquisition of very large amounts of geographic data, which should be analyzed using real-time visualization technology. The level of detail(LOD) algorithm is one of the most important elements for realizing real-time visualization. We chose the triangulated irregular network (TIN) method to generate normalized digital elevation model(DEM) data. First, we generated TIN data using contour lines obtained from a two-dimensional(2D) digital map and created a 2D grid array fitting the size of the area. Then, we generated normalized DEM data by calculating the intersection points between the TIN data and the points on the 2D grid array. We used constrained Delaunay triangulation(CDT) and ray-triangle intersection algorithms to calculate the intersection points between the TIN data and the points on the 2D grid array in each step. In addition, we simulated a three-dimensional(3D) terrain model based on normalized DEM data with real-time visualization using a Microsoft Visual C++ 6.0 program in the DirectX API library and a quad-tree LOD algorithm.

• IMMUNE NETWORK
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• v.3 no.3
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• pp.219-226
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• 2003

Background: Phage display is the most widely used technique among display methods to produce monoclonal antibody fragment with a specific binding activity. Having a large library for efficient antibody display/selection is quite laborious process to have more than $10^9$ members of transformants. To overcome these limitations, several in vitro selection approaches have been reported. Ribosome display that links phenotypes, proteins, directly to genotype, mRNA, is one of the in vitro display methods. Ribosome display can reach the size of scFv library up to $10^{14}$ molecules and it can be further diversified during PCR steps. To select the high affinity scFv from one pot library, we established ribosome display technique by modifying the previously reported eukaryotic translation system. Methods: To establish the antibody selection system by ribosome display, we used 3D8, anti-DNA antibody. A 3D8 scFv was synthesized in vitro by an in vitro transcription-translation system. The translated 3D8 scFv and the encoding 3D8 mRNA are connected to the ribosome. These scFv-ribosome-mRNA complexes were selected by binding to their specific antigens. The eluted mRNAs from the complexes are reverse transcribed and re-amplified by PCR. To apply this system, antibody library from immunized mouse with terminal protein (TP)-peptide of hepatitis B virus DNA polymerase TP domain was also used. This TP-peptide encompasses the 57~80 amino acid residues of TP. These mRNA/ribosome/scFv complexes by our system were panned three times against TP-peptide. The enrichment of antibody from library was determined by radioimmunoassay. Results: We specifically selected 3D8, anti-DNA antibody, against ssDNA as a model system. The selected 3D8 RNAs sequences from translation complexes were recovered by RT-PCR. By applying this model system, we enriched TP-peptide-specific scFv pools through three cycles of panning from immunized library. Conclusion: We show that our translating ribosome complexes are well maintained and we can enrich the TP-specific scFv pools. This system can be applied to select specific antibody from an antibody library.

• Management & Information Systems Review
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• v.28 no.3
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• pp.191-210
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• 2009

Internet was first designed only for sending data, but as the time passed, internet started to evolve into a broadband multi-media web that is capable of transmitting sound, video, high-capacity data and more due to the demands of internet users and the rapid changing internet-communication technology. Domestically, in January, 2000 Saerom C&T, launched a free VoIP, but due to limited ways of conversation(PC to PC) and absence of a revenue model, and bad speech quality, it had hit it's growth limit. This research studied VoIP based on technological enhancement in super-speed internet. According to IDC, domestic internet market's size was 80,800 million in 2008, and it formed a percentage of 12.5% out of the whole sound-communication market. in case of VoIP, it is able to maximize it's profit by connecting cable and wireless network, also it has a chance of becoming firm-concentrated monopoly market by fusing with IPTV. Considering the fact that our country is insignificant in MVNO revitalization, regulating organizations will play a significant roll on regulating profit between large and small businesses. Further research should be done to give VoIP a secure footing to prosper and become popularized.

• Convergence Security Journal
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• v.17 no.3
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• pp.41-49
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• 2017

According to a research by global IT market research institute IDC, CSIM(Converged Security Information Management) market of Korea was estimated to be 1.7 trillion KRW in 2010, and it has grown approximately 32% every year since. IDC forcasts this size to grow to 12.8 trillion KRW by 2018. Moreover, this case study exemplifies growing importance of CSIM market worldwide. Traditional CSIM solution consists of various security solutions(e.g. firewall, network intrusion detection system, etc.) and devices(e.g. CCTV, Access Control System, etc.). With this traditional solution, the the data collected from these is used to create events, which are then used by the on-site agents to determine and handle the situation. Recent development of IoT industry, however, has come with massive growth of IoT devices, and as these can be used for security command and control, it is expected that the overall amount of event created from these devices will increase as well. While massive amount of events could help determine and handle more situations, this also creates burden of having to process excessive amount of events. Therefore, in this paper, we discuss potential events that can happen in CSIM system and classify them into 3 groups, and present a model that can categorize and process these events effectively to increase overall efficieny of CSIM system.

• Journal of Environmental Science International
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• v.18 no.3
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• pp.299-308
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• 2009

We manufactured PVA-derived hydrogels using a foam generation technique that has been widely used to prepare colloidal gas aphrons(CGA). These gels were differentiated to the conventional gels such as for medical or pharmaceutical applications, which have tiny pores and some crystalline structure. Rather these should be used in de-pollution devices or adhesion of cells or biomolecules. The crosslinkers used in this work were amino acid, organic acid, sugars and lipids(vitamins). The structures of the gels were observed in a scanned electron microscope. Amino acids gels showed remarkably higher swelling ratios probably because their typical functional groups help constructing a highly crosslinked network along with hydrogen bonds. Boric acid and starch would catalyze dehydration while structuring to result in much lower water content and accordingly high gel content, leading to less elastic, hard gels. Bulky materials such as ascorbic acid or starch produced, in general, large pores in the matrices and also nicotinamide, having large hydrophobic patches was likely to enlarge pore size of its gels as well since the hydrophobicity would expel water molecules, thus leading to reduced swelling. Hydrophilicity(or hydrophobicity), functional groups which are involved in the reaction or physical linkage, and bulkiness of crosslinkers were found to be more critical to gel's cross linking structure and its density than molecular weights that seemed to be closely related to pore sizes. Microscopic observation revealed that pores were more or less homogeneous and their average sizes were $20{\mu}m$ for methionine, $10-15{\mu}m$ for citric acid, $50-70{\mu}m$ for L-ascorbic acid, $30-40{\mu}m$ for nicotinamide, and $70-80{\mu}m$ for starch. Also a sensory test showed that amino acid and glucose gels were more elastic meanwhile acid and nicotinamide gels turned out to be brittle or non-elastic at their high concentrations. The elasticity of a gel was reasonably correlated with its water content or swelling ratio. In addition, the PVA gel including 20% ascorbic acid showed fair ability of cell adherence as 0.257mg/g-hydrogel and completely degraded phenanthrene(10 mM) in 240 h.

• IMMUNE NETWORK
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• v.3 no.2
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• pp.126-135
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• 2003

Background: One of the important factors in the prognosis of chronic hepatitis B patient is the degree of replication of hepatitis B virus (HBV). It has been known that HBV DNA polymerase plays the essential role in the replication of HBV. HBV DNA polymerase is composed of four domains, TP (Terminal protein), spacer, RT (Reverse transcriptase) and RNaseH. Among these domains, tyrosine, the $65^{th}$ residue of TP is an important residue in protein-priming reaction that initiates reverse transcription. If monoclonal antibody that recognizes around tyrosine residue were selected, it could be applied to further study of HBV replication. Methods: To produce TP-specific scFv (single-chain Fv) by phage display, mice were immunized using synthetic TP-peptide contains $57{\sim}80^{th}$ amino acid residues of TP domain. After isolation of mRNA of heavy-variable region ($V_H$) and light-chain variable region ($V_L$) from the spleen of the immunized mouse, DNA of $V_H$ and $V_L$ were obtained by RT-PCR and joined by a DNA linker encoding peptide (Gly4Ser)3 as a scFv DNA fragments. ScFv DNA fragments were cloned into a phagemid vector. scFv was expressed in E.coli TG1 as a fusion protein with E tag and phage gIII. To select the scFv that has specific affinity to TP-peptide from the phage-antibody library, we used two cycles of panning and colony lift assay. Results: The TP-peptide-specific scFv was isolated by selection process using TP-peptide as an antigen. Selected scFv had 30 kDa of protein size and its nucleotide sequences were analyzed. Indirect- and competitive-ELISA revealed that the selected scFv specifically recognized both TP-peptide and the HBV DNA polymerase. Conclusion: The scFv that recognizes the TP domain of the HBV DNA polymerase was isolated by phage display.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.47 no.7
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• pp.109-117
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• 2010

Building a large-scale testbed for Future Internet is very important to evaluate a new protocol and new network architecture designed by clean-slate approach. In Korea, new Future Internet testbed project, called FIRST (Future Internet Research for Sustainable Testbed), has been started since Mar. 2009 to design and test new protocols. This project is working together with ETRI and 5 universities. The FIRST@PC is to implement a virtualized hardware-accelerated PC-node by extending the functions of NetFPGA card and build a Future Internet testbed on the KOREN and KREONET for evaluating newly designed protocols and interesting applications. In this paper, we first briefly introduce FIRST@PC project and explain a 'MAC in IP Capsulator' user-space program using raw-socket in Linux to interconnect OpenFlow enabled switch sites on the KOREN and KREONET. After that, we address test results for TCP throughput performance for varying packet size. The test results show that the software based capsulator can support a reasonable bandwidth performance for most of applications.

• Spatial Information Research
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• v.7 no.1
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• pp.1-12
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• 1999

The DTM(Digital Terrain Model) in GIS(Geographical Information System) shows the elevation from interpolation using data points surveyed. In panoramic flat landform, pixel size, resolution of source data may not be the problem in using DTM However, in mountainous landform like Korea, appropriate resolution accuracy of source data are important factors to represent the topography concerned. In this study, the difference in contour interval of source data, the resolution after interpolation, and different data structures were compared to figure out the accuracy of slope calculation using DTM from the topographic maps of Togyusan National Park Two types of GIS softwares, Idrisi(grid) ver. 2.0 using the altitude matrices and ArcView(TIN) ver. 3.0a using TIN were used for this purpose. After the analysis the conclusions are as follows: 1) The coarser resolution, the more smoothing effect inrepresenting the topography. 2) The coarser resolution the more difference between the grid-based Idrisi and the TIN-based ArcView. 3) Based on the comparison analysis of error for 30 points from clustering, there is not much difference among 10, 20, 30 m resolution in TIM-based Airview ranging from 4.9 to 6.2n However, the coarser resolution the more error for elevation and slope in the grid-based Idrisi. ranging from 6.3 to 10.9m. 4) Both Idrisi and ArcView could net consider breaklines of lanform like hilltops, valley bottoms.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.7
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• pp.750-755
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• 2003

This paper proposes a new three-line balun. The equivalent circuit of the proposed three-line balun is presented, and impedance matrix［Z］of the equivalent circuit is derived from the relationship between the current and voltage at each port. The design equation for a given set of balun impedance at input and output ports is presented using［S］parameters, which is transferred fom impedance matrix,［Z］. To demonstrate the feasibility and validity of design equation, multi-layer ceramic(MLC) chip balun operated in the 2.4 GHz ISM band frequency is designed and fabricated by the use of the low temperature co-fired ceramic(LTCC) technology. By employing both the proposed new three-line balun equivalent circuit and multi-layer configuration provided by LTCC technology, the 2012 size MLC balun is realized. Measured results of the multi-layer LTCC three-line balun match well with the full-wave electromagnetic simulation results, and measured in band-phase and amplitude balances over a wide bandwidth are excellent. This proposed balun is very easily applicable to multi-layer structure using LTCC as shown in the paper, and also can be realized with microstrip lines on PCB. This distinctive performance is very favorable for wireless communication systems such as wireless LAN(Local Area Network) and Bluetooth applications.