• 제목/요약/키워드: Nerve Fibers

검색결과 240건 처리시간 0.023초

백서 정중구개봉합 확대후의 CGRP 면역반응 신경섬유의 변화 (Changes in CGRP-immunoreactive Nerve Fibers during Expansion of Midpalatal Suture of the Rat)

  • 김보경;박국필;경희문;권오원;성재현
    • 대한치과교정학회지
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    • 제29권1호
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    • pp.73-81
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    • 1999
  • 측방확대장치에 의한 정중구개봉합부의 확대시 교정력은 구개부의 신경요소들에 변화를 일으키면서 동통을 유발하게 되고 구개봉합부가 확대되고 그것이 유지되는 동안에 기계적 자극에 의한 구개부신경섬유의 반응성에 변화를 일으킬 것으로 생각된다. 본 연구에서는 백서 정중구개봉합부의 CGRP 면역반응 신경섬유의 교정력 적용시간에 따른 형태학적인 반응성, 밀도, 분포변화등을 관찰하고 그와 연관된 CGRP의 기능을 알아보고자 250gm내외의 Sprague-Dawley 웅성백서 상악전치에 200gm의 치아이개력이 가해지도록 활성화시킨 helical spring을 삽입하여 정상대조군과 장치 장착후 경과시간에 따라 1일, 4일, 7일, 14일 경과군으로 나누어 정중구개봉합부를 확대후 희생시켜 면역조직화학법으로 염색하여 관찰하였다. ${\cdot}$ 대조군에서 정중구개봉합부 결합조직의 CGRP 면역양성 신경섬유는 거의 관찰되지 않았다. ${\cdot}$1일군에서는 대조군에 비해 CGRP면역반응 신경섬유가 현저한 증가를 보였으며 주로 혈관 주위에 염주알 모양의 가는 신경섬유가 관찰되었다. ${\cdot}$ 4일군에서는 CGRP면역반응 신경섬유가 대조군에 비해서는 현저한 증가를 보였으나 1일군에 비해서는 크게 증가하지 않았으며 혈관의 크기가 훨씬 확장되어 관찰되었다. ${\cdot}$ 7일군에서는 특징적으로 조골양세포가 새로이 형성된 골변연을 따라 줄지어 배열되는 독특한 양상이 관찰되었고 신경섬유의 수는 4일군에 비하여 감소하고 혈관의 직경도 감소되었다. ${\cdot}$14일군에서는 CGRP면역반응 신경섬유가 7일군과 유사한 분포를 보였으며 확대에 의한 골변연의 불규칙성도 대체로 감소되었다. CGRP면역반응 신경섬유는 정중구개봉합의 확대시 초기에 일어나는 신경원성 염증반응에 주로 관련되어 증식되는 것으로 생각된다.

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출생후 흰쥐 구치의 발육에 따른 치수의 신경지배 발달에 관한 면역조직화학적 연구 (IMMUNOHISTOCHEMICAL STUDY ON THE POSTNATAL DEVELOPMENT OF DENTAL PULP INNERVATION IN RAT POSTERIOR TEETH)

  • 김동길;김현정;남순현;배용철;김영진
    • 대한소아치과학회지
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    • 제23권4호
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    • pp.775-787
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    • 1996
  • The purpose of this study was to investigate the postnatal development of pulpal innervation with the tooth development and eruption process in developing rat mandibular molars (postnatal 1, 5, 10, 15, 20, 25, 35day-old and adult rats). Immunohistochemical method was carried out for the detection of nerve fibers, using the antibody against calcitonin gene-related peptide(CGRP). The results were as follows: The feature of CGRP-IR nerve fibers were shown in a bead-like appearance. The time of nerve entering into the dental papilla of tooth follicle began at the occured advanced dentinogenesis. The development of Raschkow plexus began at the formative stage of the roots and was accelerated at the stage of the crown emerged into the oral cavity. The number of nerve fibers entering the odontoblastic layer increased with the tooth eruption and mastication. The development of innervation was shown to be related with the stage of the development of individual teeth rather than the chronological age of the rat.

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열통과 열자극 (Thermal Pain and Thermal Stimulus)

  • 곽재희;곽영세
    • The Korean Journal of Physiology
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    • 제7권1호
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    • pp.49-51
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    • 1973
  • Denuded nerve fibers containing the node of Ranvier were prepared from the nerve innervating the gastrocnemius muscle of the frog. The effects of temperature rise of the medium surrounding the naked nerve fibers were studied upon the muscle response. A rise in temperature of the order of $50^{\circ}{\sim}90^{\circ}C$ was proved ineffective in the production of contractions in the muscle. It is thus concluded that the widespread concept that heating is one of the nerve stimuli is inadequate, and that thermal pain is the indirect effect of thermal stimuli. The authors' standpoint about thermal stimulus is that thermal stimulus or heating is one thing, cooling is another.

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모세포 손상 가묘에 대한 Cochlear Implant (Cochlear Implant of the Hair-Cell Damaged Cats)

  • 장인원;김성남;양한모;최윤호;조용범
    • 대한기관식도과학회:학술대회논문집
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    • 대한기관식도과학회 1978년도 제12차 학술대회연제 순서 및 초록
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    • pp.9.1-10
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    • 1978
  • Action potential의 본체는 cochlear nerve의 섬유집합의 주기적 흥분을 보이는 전위변화(복합활동전위)를 말한다. 와우신경섬유의 흥분인 impulse wave-form은 음양(N.P,)의 bipolar wave-form을 나타내기 때문에 A.P.도 이상성 wave-form으로 나타난다. 기계적으로나 약물로 내외모세포를 파괴한 가묘의 고실계에 induction coil을 삽입하고 동시에 outer device를 통해서 준 음자극에 의하여 얻은 파형을 분석 보고한다.

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출생후 성장발육에 따른 흰쥐 유곽유두의 CGRP 면역양성 신경분포변화 (POSTNATAL DEVELOPMENT OF CGRP-IMMUNOREACTIVE NERVE DISTRIBUTION OF RAT CIRCUMVALLATE PAPILLA)

  • 이재문;김현정;배용철;김영진
    • 대한소아치과학회지
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    • 제24권2호
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    • pp.339-351
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    • 1997
  • 출생 후 성장 발육에 따른 흰쥐 유곽유두의 신경분포 및 변화를 관찰하기 위하여 생후 1, 5, 10, 15, 20, 25, 35일 및 완전히 성장한 흰쥐를 대상으로 calcitonin gene-related peptide (CGRP)를 이용한 면역조직화학적 방법으로 관찰하여 다음과 같은 결과를 얻었다. CGRP-면역양성 신경섬유들이 혀의 양측에서부터 유두 내로 진입하여 성장발육에 따라 그 분포밀도가 증가하다가 10일군부터는 상피의 직하방에 특히 풍부하게 분포하여 subepithelial plexus를 형성하고 있었다. 1일군에서는 CGRP-면역양성 신경섬유의 특징적인 양상인 염주알 모양이 명확히 관찰되었으나 그 이후로는 염주알 모양은 다소 감소하였고 하나의 선으로 보이는 경우도 있었다. 상피 내로 진입하는 CGRP-면역양성 신경섬유들은 5일군에서 드물게 관찰되었으며 taste pore를 가진 성숙한 미뢰가 처음으로 관찰되는 10일군에서 그 수가 증가하여 출생 후 15일군에서는 상피 전반에 걸쳐 풍부하게 관찰되었다. 그러나 그 이후 20일군부터는 미뢰가 존재하는 trench wall의 하방 2/3부위의 CGRP-면역양성 신경섬유는 감소하고 유두의 top surface와 trench wall의 상방 1/3에 CGRP-면역양성 신경섬유가 제한적으로 관찰되었다.

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가토 하치조신경 재건에 있어 정맥이식통로를 이용한 신경재생유도에 관한 실험적 연구 (VEIN GRAFT REPAIR COMPARED WITH NERVE GRAFTING FOR INFERIOR ALVEOLAR NERVE REGENERATION IN RABBITS)

  • 신일;김명진;남일우
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제26권3호
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    • pp.270-278
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    • 2000
  • This study was conducted to compare the vein graft with the nerve graft, and evaluated the availability of the vein graft on the reconstruction of the inferior alveolar nerve defect. The experimental animals were 12 rabbits weighing $1.5{\sim}2.0kg$, divided into 3 groups : sham operation group, vein conduit group and nerve graft group. All nerves were excised and histomorphometric analysis was performed at 2, 4, 6, 12, 16 weeks after operation. The obtained results were as follows. 1. Histologic examination revealed the regenerated nerve fibers within the lumen of the vein graft and nerve graft at 6 weeks after repair. 2. Axon diameter was significantly larger in nerve graft group(p<0.05) than in vein graft group at 6weeks, and larger in nerve graft group than in vein graft group at 16weeks. 3. Axon density was higher in the vein graft group at 16 weeks. 4. The myelin of the regenerated nerve fibers in distal segment of the vein graft group was thick, approaching the proximal segment at 16weeks. This means remyelination in distal segment in the vein graft group. These results suggested that autogenous vein graft may be used as an alternative to autogenous nerve graft.

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개의 횡격막신경섬유(橫隔膜神經纖維)에 관(關)한 연구(硏究) 제(第)II보(報) 횡격막신경(橫隔膜神經)의 횡격막내분기(橫隔膜內分技)의 유수섬유(有髓纖維)에 관(關)하여 (Studies on the Phrenic Nerve Fibers in the dog II. On the Myelinated Fibers of the Intradiaphragmatic Branches)

  • 고광두;윤석봉;이준섭
    • 대한수의학회지
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    • 제9권1호
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    • pp.1-9
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    • 1969
  • The experimental studies were performed to observe the characteristics of the myelinated nerve fibers of the tradiaphragmatic branches of phrenic nerves in dog In the work to be reported five mixed breed Korean dog were used, they were one year old and he body eights were about 12 Kgm. in average with healthy conditions. The specimens (nerve) were taken at the point of 1.5 cm posterior part from the left and right phrenic nerves entrance to the diaphragm, and the dorso-lateral and dorso-medial branches of phrenic nerves vere also, taken at the point of 0.5 cm distal part from their branching portion. The specimens were fixed for 24 hrs. in Flemming's solution, and embedded in paraffin. The paraffin section. were cut at 6 microns and stained with Walter's modification of Weigert-pal method for the myelinated fibers. Microphotographs were taken and enlarged into 750 times of the actual size, and the diameter of the photographic images of the myelinated fibers were measured by the scale on the transparent Percepex Plate. The following conclusions were made: 1. The percentage of distribution of the several intradiaphragmatic branches of phrenic nerves were as follows. Ventral branches were 34.97%, lateral branches were 37.90%, dorsal branches were 27.13%, and the dorsal branches were branched again into dorso-lateral branches(54.22%) and dorso-medial branches (45. 78%). 2. The mean value and S.D. of the total numbers of the myelinated nerve fibers at the left ventral branches were $490.80{\pm}12$, and at the right ventral branches were $486.6{\pm}13$. Total average cross sectional area on the left ventral branches were 38,000. $6{\pm}136{\mu}^2$, and the right ventral branches were $37,150.2{\pm}1.782{\mu}^2$. 3. The mean value and S.D. of the total numbers of the myelinated nerve fibers at the left lateral branches were $533.8{\pm}8$, and at the right lateral branches were $525.6{\pm}7$. Total average cross sectional area of the left lateral branches were $41,582{\pm}1,170{\mu}^2$, and the right lateral branches were $40,454.8{\pm}812{\mu}^2$. 4. The mean value and S.D. of the total numbers of the myelinated nerve fibers at the left dorsal branches were $378.2{\pm}14$, and at the right dorsal branches were $380.2{\pm}8$. Total average cross sectional area of the left dorsal branches were $27,771{\pm}1,256{\mu}^2$, and the right dorsal branches were $27,507.2{\pm}645{\mu}^2$. 5. The mean value and S.D. of the total numbers of the myelinated fibers at the left dorso-lateral branches were $205.4{\pm}15$, and at the right dorso-lateral branches were $210.6{\pm}17$. Total average cross sectional area of the left dorso lateral branches were $15,354. 8{\pm}1,519{\mu}^2$, and the right dorsal branches were $15,887{\pm}1,297{\mu}^2$. 6. The mean value and S.D. of the total numbers of the myelinated nerve fibers at the left dorso-medial branches were $175{\pm}14$, and at the right dorso-medial branches were $176.2{\pm}17$. Total average cross sectional area of the left dorso-medial branches were $13,037.4{\pm}944{\mu}^2$, and at the right dorso-medial branches were, $13,103{\pm}1,373{\mu}^2$. 7. The highest frequent distribution of the intradiaphragmatic branches of phrenic nerves were found in the $10-12{\mu}^2$ groups, and they were almost 30% of the total groups. 8. The largest fibers diameter were in the $14-16{\mu}^2$ groups, and these were shown the lowest frequent distribution. 9. The largest cross sectional area of the intradiaphragmatic branches of phrenic nerves were found in the $10-12{\mu}^2$ groups. 10. All of the intradiaphragmatic branches of phrenic nerves were unimodal which has a peak in the $10-12{\mu}^2$ groups.

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영아 비후성 유문협착증에서 유문부의 신경분포의 비교 (Comparative Nerve Distribution of the Pylorus in Infantile Hypertrophic Pyloric Stenosis)

  • 김강도;주대현;유용운;박성환;박기호;박재복
    • Advances in pediatric surgery
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    • 제8권1호
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    • pp.23-27
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    • 2002
  • Infantile hypertrophic pyloric stenosis (IHPS) a common childhood disorders characterized by nonbilious projectile vomiting, an olive shaped mass in the right upper quadrant of the abdomen and visible gastric peristaltic wave in the upper abdomen. Its etiology and pathogenesis are not clear but abnormal nerve distribution of the pylorus has been $postulated^{2-6}$. We performed immunocytochemical staning to the pyloric muscle from 10 IHPS and 3 controls patients, utilizing specific monoclonal antibody to NCAM(neural cell adhesion molecule). In IHPS patients, the number of NCAM protein immunoreactive nerve fibers were less than that in normal subjects. Auerbach myenteric plexuse was well developed and interbundle nerve plexuse was present but nerve fibers supplying individual muscle cells in smooth muscle bundles were poorly developed. These results indicate reduction of innervation in smooth muscles in IHPS patients that possibly contributes to the pathogenesis of IHPS.

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갈근(葛根)이 좌골신경 손상 흰쥐의 후지 근육위축에 미치는 영향 (Effect of Puerariae Radix on Hind Limb Muscle Atrophy of Sciatic Nerve Transectioned Rats)

  • 장승욱;김연섭
    • 동의생리병리학회지
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    • 제23권2호
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    • pp.405-411
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    • 2009
  • This study evaluated the effects of Puerariae Radix on the skeletal muscle atrophy, Muscle atrophy was induced by the sciatic nerve transection in Sprague-Dawley rats, then aqueous-extract of Puerariae Radix was administered for 12 days, Muscle wet weight was measured in soleus, plantaris, and medial gastrocnemius. Muscle fiber type was classified by MHCf immunohistochemistry. Muscle fiber type proportion and cross section area of muscle fiber also was observed in medial gastrocnemius. Bax and Bcl-2 expressions in medial gastrocnemius of the damaged hind limb were evaluated with immunohistochemistry. The results are as follows; Puerariae Radix attenuated muscle atrophy in soleus of the sciatic nerve transectioned rats, but there was statistic significance. Puerariae Radix attenuated significantly atrophy in plantaris at 12 days and in medial gastrocnemius at 8 days and 12 days. Puerariae Radix improved histology of the atrophic changes and increased significantly cross section areas of type-I and type-II muscle fibers in medial gastrocnemius of the sciatic nerve transectioned rats. Puerariae Radix did not affect to muscle fiber type proportion in medial gastrocnemius of the sciatic nerve transectioned rats. Puerariae Radix attenuated significantly Bax positive nuclei but did not affect to Bcl-2 positive muscle fibers in medial gastrocnemius of the sciatic nerve transectioned rats.According to above results, Puerariae Radix may have an anti-atrophy effect on the denervated skeletal muscle through anti-apoptotic effects on muscle fibers.

CGRP, SP의 국소도포와 전기자국에 의한 고양이 치수신경활동의 변화 (CHANGES IN INTRADENTAL NERVE ACTIVITY FOLLOWING LOCAL APPLICATION OF CGRP, SP AND ELECTRICAL STIMULI IN CATS)

  • 김미자;손호현
    • Restorative Dentistry and Endodontics
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    • 제20권2호
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    • pp.527-537
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    • 1995
  • The effect of calcitonin gene-related peptide (CGRP), substance P (SP) and electrical stimulation of the tooth on the intradental nerve activtiy (INA) was investigated in anesthetized cats. The INA was recorded from single pulp nerve units dissected from the inferior alveolar nerve under stereomicroscope. The INA elicited by 3 minute application of 4M NaCl in deep dentinal cavity was compared before and after stimulation at 10 minute intervals. The magnitude of INA was calculated as the total number of nerve impulses produced in given period, and the changes of INA are expressed as % of control INA. The results obtained were as follows. 1. 16 single pulp nerve units were classified as 14 $A{\delta}$-fibers (3.4~19.4m/sec) and 2-fibers (1.5~1.7m/sec) according to the conduction velocity. 2. 4M NaCl evoked an irregular bursts of spikes which continued until washing out. Isotonic saline did not affect INA to subsequent applications of the hypertonic NaCl solution (P>0.05). 3. Local application of CGRP ($200{\mu}g$/ml) in deep dentinal cavity reduced the INA induced by 4M NaCl in $A{\delta}$-fiber units (P<0.01) and some units of those responded to CGRP during application. 4. Local application of SP ($100{\mu}g$/ml) in deep dentinal cavity reduced the INA induced by 4M NaCl in AS-fiber units (p<0.05), but increased the INA in C-fiber unit coincided with large reduction of the INA of $A{\delta}$-fiber units. 5. Monopolar electrical stimulation applied to the crown at intensities high enough to excite C-fibers (12V, 5ms, 10Hz, 10~30min) decreased the INA in $A{\delta}$-fiber units (P<0.01) and systemic pretreatment with phenoxybenzamine (3mg/kg, i.v.) enhanced this inhibitory effect (P<0.01). On the contrary, electrical stimulation increased the INA in C-fiber unit.

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