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Effect of Puerariae Radix on Hind Limb Muscle Atrophy of Sciatic Nerve Transectioned Rats  

Jang, Sung-Wook (Department of Anatomy-Pointlogy, College of Oriental Medicine, Kyungwon University)
Kim, Youn-Sub (Department of Anatomy-Pointlogy, College of Oriental Medicine, Kyungwon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.2, 2009 , pp. 405-411 More about this Journal
Abstract
This study evaluated the effects of Puerariae Radix on the skeletal muscle atrophy, Muscle atrophy was induced by the sciatic nerve transection in Sprague-Dawley rats, then aqueous-extract of Puerariae Radix was administered for 12 days, Muscle wet weight was measured in soleus, plantaris, and medial gastrocnemius. Muscle fiber type was classified by MHCf immunohistochemistry. Muscle fiber type proportion and cross section area of muscle fiber also was observed in medial gastrocnemius. Bax and Bcl-2 expressions in medial gastrocnemius of the damaged hind limb were evaluated with immunohistochemistry. The results are as follows; Puerariae Radix attenuated muscle atrophy in soleus of the sciatic nerve transectioned rats, but there was statistic significance. Puerariae Radix attenuated significantly atrophy in plantaris at 12 days and in medial gastrocnemius at 8 days and 12 days. Puerariae Radix improved histology of the atrophic changes and increased significantly cross section areas of type-I and type-II muscle fibers in medial gastrocnemius of the sciatic nerve transectioned rats. Puerariae Radix did not affect to muscle fiber type proportion in medial gastrocnemius of the sciatic nerve transectioned rats. Puerariae Radix attenuated significantly Bax positive nuclei but did not affect to Bcl-2 positive muscle fibers in medial gastrocnemius of the sciatic nerve transectioned rats.According to above results, Puerariae Radix may have an anti-atrophy effect on the denervated skeletal muscle through anti-apoptotic effects on muscle fibers.
Keywords
Puerariae Radix; soleus; plantaris; MHCf; Bax; Bcl-2;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 이상인, 안덕균, 신민교. 한약임상응용. 서울, 성보사, pp 370-372, 1982
2 김호철. 한약약리학. 서울, 집문당, pp 92-94, 2001
3 김상현, 김연섭. 갈근의 뇌해마 신경세포 손상보호와 항산화 효능에 대한 연구. 동의생리병리학회지 19(2):416-425, 2005   과학기술학회마을
4 Muller, F.L, Song, W., Jang, Y.C., Liu, Y., Sabia, M., Richardson, A., Van Remmen, H. Denervation-induced skeletal muscle atrophy is associated with increased mitochondrial ROS production. Am J Physiol Regul Integr Comp Physiol. 293(3):R1159-1168, 2007   DOI   PUBMED   ScienceOn
5 Arguello, A., Lopez-Fernandez, J.L., Rivero, J.L. Limb myosin heavy chain isoproteins and muscle fiber types in the adult goat (Capra hircus). Anat Rec. 264(3):284-293, 2001   DOI   ScienceOn
6 Keung, W.M., Vallee, B.L. Daidzin: a potent, selective inhibitor of human mitochondrial aldehyde dehydrogenase. Proc Natl Acad Sci USA. 90(4):1247-1251, 1993   DOI   ScienceOn
7 Keung, W.M., Klyosov, A.A., Vallee, B.L. Daidzin inhibits mitochondrial aldehyde dehydrogenase and suppresses ethanol intake of Syrian golden hamsters. Proc Natl Acad Sci USA. 94(5):1675-1679, 1997   DOI   ScienceOn
8 김연섭. 갈근이 뇌허혈 손상 흰쥐의 해마 구역별 HSP70 발현에 미치는 영향. 동의생리병리학회지 18(1):167-171, 2004   과학기술학회마을
9 Guang, H.M., Zhang, X.M., Li, Y.Q., Wei, X.B., Wang, Z.Y., Liu, H.Q. Protective effects of hydroxyethylpuerarin on cultured bovine cerebral microvascular endothelial cells damaged by hydrogen peroxide. Yao Xue Xue Bao. 40(3):220-224, 2005   PUBMED
10 Gillespie, M.J., Gordon, T., Murphy, P.R. Motor units and histochemistry in rat lateral gastrocnemius and soleus muscles: evidence for dissociation of physiological and histochemical properties after reinnervation. J Neurophysiol, 57(4):921-937, 1987   DOI   PUBMED
11 Haddad, F., R.E. Herrick, G.R. Adams and K.M. Baldwin. Myosin heavy chain expression in rodent skeletal muscle: effects of exposure to zero gravity. J Appl Physiol. 75: 2471-2477, 1993   DOI
12 Liu, M., Zhang, D., Shao, C., Liu, J., Ding, F., Gu, X. Expression pattern of myostatin in gastrocnemius muscle of rats after sciatic nerve crush injury. Muscle Nerve. 35(5):649-656, 2007   DOI   ScienceOn
13 Nicholso, D.W. Mechanisms of apoptotic control. Nature. 407: 810-816, 2000   DOI   PUBMED   ScienceOn
14 Kim, O.S., Choi, J.H., Soung, Y.H., Lee, S.H., Lee, J.H., Ha, J.M., Ha, B.J., Heo, M.S., Lee, S.H. Establishment of in vitro test system for the evaluation of the estrogenic activities of natural products. Arch Pharm Res. 27(9):906-911, 2004   DOI   ScienceOn
15 Jang, M.H., Shin, M.C., Lee, T.H., Bahn, G.H., Shin, H.S., Lim, S., Kim, E.H., Kim, C.J. Effect of Puerariae radix on c-Fos expression in hippocampus of alcohol-intoxicated juvenile rats. Biol Pharm Bull. 26(1):37-40, 2003   DOI   ScienceOn
16 Arao, T., Udayama, M., Kinjo, J., Nohara, T. Preventive effects of saponins from the Pueraria lobata root on in vitro immunological liver injury of rat primary hepatocyte cultures. Planta Med. 64(5):413-416, 1998   DOI   ScienceOn
17 Chen, J., Graham, S.H., Chan, P.H., Lan, J., Zhou, R.L, Simon, R.P. Bcl-2 is expressed in neurons that survive focal ischemia in the rat. Neuroreport. 6: 394-398, 1995   DOI   PUBMED   ScienceOn
18 Olive, M., Ferrer, L. Bcl-2 and Bax protein expression in human myopathies. J Neurol Sci. 164: 76-81, 1999   DOI   ScienceOn
19 Goldspin,k D.F. The effects of denervation on protein turnover of rat skeletal muscle. Biochem J. 156: 71-80, 1976   DOI
20 Jankala, H., V.P. Harjola, N.E. Petersen and M. Harkonen. Myosin heavy chain mRNA transform to faster isoforms in immobilized skeletal muscle: a quantitative PCR study. J Appl Physiol. 82: 977-982, 1997   DOI
21 Wang, X., Wu, J., Chiba, H., Umegaki, K., Yamada, K., Ishimi, Y. Puerariae radix prevents bone loss in ovariectomized mice. J Bone Miner Metab. 21(5):268-275, 2003   DOI   ScienceOn
22 Arao, T., Kinjo, J., Nohara, T., Isobe, R. Oleanene-type triterpene glycosides from puerariae radix. IV. Six new saponins from Pueraria lobata. Chem Pharm Bull. 45(2):362-366, 1997   DOI   PUBMED   ScienceOn
23 Megighian, A., Germinario, E., Rossini, K., Midrio, M., Danieli-Betto, D. Nerve control of type 2A MHC isoform expression in regenerating slow skeletal muscle. Muscle Nerve. 24(1):47-53, 2001   DOI   ScienceOn
24 Jiang, R.W., Lau, K.M., Lam, H.M., Yam, W.S., Leung, L.K., Choi, K.L., Waye, M.M., Mak, T.C., Woo, K.S., Fung, K.P. A comparative study on aqueous root extracts of Pueraria thomsonii and Pueraria lobata by antioxidant assay and HPLC fingerprint analysis. J Ethnopharmacol. 96(1-2):133-138, 2005   DOI   ScienceOn
25 Guerra, M.C., Speroni, E., Broccoli, M., Cangini, M., Pasini, P., Minghett, A., Crespi-Perellino, N., Mirasoli, M., Cantelli-Forti, G., Paolini, M. Comparison between chinese medical herb Pueraria lobata crude extract and its main isoflavone puerarin antioxidant properties and effects on rat liver CYP-catalysed drug metabolism. Life Sci. 67(24):2997-3006, 2000   DOI   ScienceOn
26 Jang, M.H., Shin, M.C., Chung, J.H., Shin, H.D., Kim, Y., Kim, E.H., Kim, C.J. Effects of Puerariae radix on cell proliferation and nitric oxide synthase expression in dentate gyrus of alcohol-intoxicated Sprague-Dawley rats. Jpn J Pharmacol. 88(3):355-358, 2002   DOI   ScienceOn
27 Guang, H.M., Zhang, X.M., Li, Y.Q., Wei, X.B., Wang, Z.Y., Liu, H.Q. Protective effects of hydroxyethylpuerarin on cultured bovine cerebral microvascular endothelial cells damaged by hydrogen peroxide. Yao Xue Xue Bao. 40(3):220-224, 2005   PUBMED
28 Lu, D.X., Huang, S.K., Carlson, B.M. Electron microscopic study of long-term denervated rat skeletal muscle. Anat Rec. 248: 355-365, 1997.   DOI   ScienceOn
29 Finol, H.J., Lewis, D.M., Owens, R. The effects of denervation on contractile properties or rat skeletal muscle. J Physiol. 319: 81-92, 1981   DOI   PUBMED
30 Li, Y.P., Atkins, C.M., Sweatt, J.D., Reid, M.B. Mitochondria mediate tumor necrosis factor-alpha/NF-kappaB signaling in skeletal muscle myotubes. Antioxid Redox Signal. 1(1):97-104, 1999.   DOI   ScienceOn
31 Jakubiec-Puka, A., Kordowska, J., Catani, C., Carraro, U. Myosin heavy chain isoform composition in striated muscle after denervation and self-reinnervation. Eur J Biochem. 193: 623-628, 1990   DOI   ScienceOn
32 Ferreira, R., Vitorino, R., Neuparth, M.J., Appell, H.J., Amado, F., Duarte, J.A. Cellular patterns of the atrophic response in murine soleus and gastrocnemius muscles submitted to simulated weightlessness. Eur J Appl Physiol. 101(3):331-340   DOI   ScienceOn
33 Rong, H., Stevens, J.F., Deinzer, M.L., Cooman, L.D., Keukeleire, D.D. Identification of isoflavones in the roots of Pueraria lobata. Planta Med. 64(7):620-627, 1998   DOI   ScienceOn
34 이상인. 본초학. 서울, 의학사, pp 65-66, 1975
35 Dupont-Versteegden, E.E. Apoptosis in skeletal muscle and its relevance to atrophy. World J Gastroenterol. 12(46):7463-7466, 2006   DOI
36 Keung, W.M. Biochemical studies of a new class of alcohol dehydrogenase inhibitors from Radix puerariae. Alcohol Clin Exp Res. 17(6):1254-1260, 1993   DOI   ScienceOn
37 Gupta, R.C., Misulis, K.E., Dettbarn, W.D. Activity dependent characteristics of fast and slow muscle: biochemical and histochemical considerations. Neurochem Res, 14(7):647-655, 1989   DOI   PUBMED
38 Chatzisotiriou, A.S., Kapoukranidou, D., Gougoulias, N.E., Albani, M. Effect of neonatal spinal transection and dorsal rhizotomy on hindlimb muscles. Brain Res Dev Brain Res. 157(2):113-123, 2005   DOI   ScienceOn
39 Dupont-Versteegden, E.E., Houle, J.D., Gurley, C.M. and Peterson, C.A. Early changes in muscle fiber size and gene expression in response to spinal cord transection and exercise. Am J Physiol Cell Physiol, 275: C1124-C1133, 1998   DOI
40 Kang, K.A., Chae, S., Koh, Y.S., Kim, J.S., Lee, J.H., You, H.J., Hyun, J.W. Protective effect of puerariae radix on oxidative stress induced by hydrogen peroxide and streptozotocin. Biol Pharm Bull. 28(7):1154-1160, 2005   DOI   ScienceOn