• 한국균학회지
• /
• 제47권1호
• /
• pp.89-94
• /
• 2019

중국에서 범부채의 녹병균이 Puccinia iridis로 동정됨에 따라 우리나라에서도 범부채의 녹병균을 재검토하였다. 저자들이 채집한 2점의 시료를 형태적으로 검토한 결과 모두 P. iridis의 특징과 일치하였다. 또한 유전분석한 결과 ITS 및 LSU rDNA 영역의 염기서열이 기존에 기록된 P. iridis와 각각 100% 및 99%의 상동성을 나타냈다. 이를 Neighbor-joining 분석법으로 계통수를 작성하였을 때도 P. iridis 계통군에 속하였다. 따라서 우리나라에서 범부채의 녹병균으로 P. iridis의 존재가 확인되었다. 한편, 우리나라에서 2003년에 범부채의 녹병균으로 기록된 Puccinia belamcandae에 대한 재검토는 향후 숙제로 남게 되었다.

• 한국자원식물학회지
• /
• 제35권1호
• /
• pp.100-108
• /
• 2022

한반도 미기록 외래식물인 서양쐐기풀(쐐기풀과)를 한강 수계에서 발견하였다. 서양쐐기풀은 유럽, 온대 아시아, 서북아프리카 원산으로 알려져 있으며, 세계의 여러 지역에 도입되어 있는데 특히, 동아시아(일본), 오세아니아, 남아메리카에서 외래종으로 보고되었다. 본 분류군은 단성화이며, 자웅이주를 가지는 특징에 의해 근연분류군과 구분된다. Neighbor-Joining 계통수에서 국내 난지한강공원과 양재천에서 채집된 서양쐐기풀은 U. dioica s.s.군으로 유집되었다. 주요 형질에 대한 기재문과 화상자료 및 검색표를 제시하였다.

• Genomics & Informatics
• /
• 제9권4호
• /
• pp.181-188
• /
• 2011

In planning a model-based phylogenic study for highly related ethnic data, the SNP marker number is an important factor to determine for relationship inferences. Genotype frequency data, utilizing a sub sampling method, from 63 Pan Asian ethnic groups was used for determining the minimum SNP number required to establish such relationships. Bootstrap random sub-samplings were done from 5.6K PASNPi SNP data. DA distance was calculated and neighbour-joining trees were drawn with every re-sampling data set. Consensus trees were made with the same 100 sub-samples and bootstrap proportions were calculated. The tree consistency to the one obtained from the whole marker set, improved with increasing marker numbers. The bootstrap proportions became reliable when more than 7,000 SNPs were used at a time. Within highly related ethnic groups, the minimum SNPs number for a robust neighbor-joining tree inference was about 7,000 for a 95% bootstrap support.

• Animal Systematics, Evolution and Diversity
• /
• 제29권1호
• /
• pp.18-22
• /
• 2013

A recent study on the mitochondrial genetic variation of the Carassius auratus population in South Korea suggested that there are 3 distinct mitochondrial lineages in the country, and that they are geographically separated between westward rivers and southward rivers, respectively. In this study, the population genetic structure of amplified fragment length polymorphism (AFLP) of Carassius auratus was investigated. The results of analysis of molecular variance (AMOVA) supported the geographic distinction between westward and southward river populations, but only 3.66% of total genetic variance lies among these populations. The panmicticity of the AFLP genetic variation is backed up by the results of the neighbor-joining dendrogram drawn from a linearized pairwise $F_{ST}$ matrix and Bayesian clustering analysis. The discordance of genetic structure between mitochondrial and AFLP genetic variation may come from difference in effective population size between these markers and/or gene flow between westward and southward river populations through river capture events.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권12호
• /
• pp.1696-1700
• /
• 2005

Five China native cattle breeds have been characterized by using 10 microsatellite DNA markers. The studied populations can be divided into five groups: Luxi cattle, Nanyang cattle, Jinnan cattle, Qinchuan cattle and Yanbian cattle. Allele frequencies were calculated and used for the characterization of the breeds and the study of their genetic relationships. Heterozygosity, polymorphism information content, the effective number of alleles was calculated. Nei' standard genetic distance (1978) was calculated and used for a neighbor-joining tree construction. NJ tree showed that Luxi cattle, Nanyang cattle, Jinnan cattle and Qinchuan cattle are closely related, whereas Yanbian cattle are clearly distinct from other four populations. The genetic relationship of five breeds corresponds to their history and geographic origins. This work analyzes the recent origin of these populations and contributes to the knowledge and genetic characterization of China native breeds.

• Journal of Life Science
• /
• 제11권1호
• /
• pp.34-38
• /
• 2001

The cDNA encoding ribosomal protein S4(RPS 4) from an ovary cDNA library of the Japanese monkey (Macaca fuscata) was cloned and sequenced. The RPS4X gene from monkey X chromosome encodes a deduced protein of 263 amino acids and share 99.1% cDNA sequence similarity and 100% amino acid sequence identify with the human RPS4X. Rate of synonymous substitution was higher in RPS4Y than in RPS4X in comparison to the monkey and human. The ratio of synonymous and nonsynonymous substitutions per site indicated that directional selection has nor occurred in RPS4 genes. Phylogenetic analysis using the neighbor-joining method revealed that X and Y-linked RPS4 genes have evolved independently.

• Molecules and Cells
• /
• 제19권2호
• /
• pp.283-288
• /
• 2005

Complete 18S rDNA sequences were determined for 10 vetigastropods in order to investigate the phylogeny of Vetigastropoda, which is controversial. These sequences were analyzed together with published sequences for nine other vetigastropods and two nerites. With the two nerites as outgroups, the phylogeny was inferred by three analytical methods, neighbor-joining, maximum likelihood, and maximum parsimony. The 18S rDNA sequence data support the monophyly of four vetigastropod superfamilies, the Pleurotomarioidea, the Fissurelloidea, the Haliotoidea, and the Trochoidea. The present results yield the new branching order: (Pleurotomarioidea (Fissurelloidea ((Scissurelloidea, Lepetodriloidea) (Haliotoidea, Trochoidea)))) within the vetigastropod clade.

• 한국미생물생명공학회:학술대회논문집
• /
• 한국미생물생명공학회 2000년도 Proceedings of 2000 KSAM International Symposium and Spring Meeting
• /
• pp.128-134
• /
• 2000

In the course of flora analysis of soil Archaea, we found very strange 16S rDNA clones, which could possibly constitute a sister clade from known two archael, Crenarchaeota and Euryarchaeota, lineages. Overall signature sequences showed that the clones were closely related to domains Archaea and Eucarya. However, at least nine nucleotides distinguished the novel clones from domains Archaea and Eucarya. Phylogenetic trees drawn by maximum parsimony, neighbor joining and maximum likelihood methods also showed unique phylogenetic position of the clones. A very specific primer set was synthesized to detect the presence of the novel group of organisms in terrestrial environments. A specific DNA fragment was amplified from all of paddy soil DNAs, and this fact suggests that the novel organisms inhabit paddy soils.

• 한국어류학회지
• /
• 제21권sup1호
• /
• pp.75-75
• /
• 2009

The previous morphology-based taxonomic frameworks within the family Sygnathidae had emphasized the significance of the male brood pouch and reproductive biology in defining the group. However, several different hypotheses had been proposed by different investigators. This study have beencarried out to determine the phylogenetic relationships among 19 species belonging to the order Syngnathiformes with three Gasterosteiformes species as outgroup taxa by using the mitochondrial cytochrome b and Rag2 nuclear DNA sequences. Phylogenetic analyses based on neighbor-joining distance, maximum parsimony, minimum evolution and maximum likelihood method strongly supported that the family Syngnathidae, the suborder Syngnathoidei and the order Syngnathiformes were all monophyletic group. Much of previous morphological analyses were supported by our molecular data, but some deep relationships were not clearly resolved with regard to members of the suborder Aulostomoidei.

• Animal cells and systems
• /
• 제6권1호
• /
• pp.81-84
• /
• 2002

SINE-R retroposons have been derived from human endogenous retrovirus HERV-K family and found to be hominoid specific. Both SINE-R retroposons and HERV_K family are potentially capable of affecting the expression of closely located genes. Using the genomic DNA from patients with schizophrenia, we identified 26 SINE-R retroposons and analyzed them with the sequences derived from the hominoid primates. The SINE-R retroposons from schizophrenia showed 89.7-96.6％ sequence similarities with the sequence of the schizo-cDNA clone that derived from postmortem tissue from the frontal cortex of an individual suffering from schizophrenial. Phylogenetic analysis using the neighbor-joining method revealed that the new SINE-R retroposons in schizophrenia have proliferated independently during hominid evolution. Such retroposons have great relevance to genomic change connected to human diseases. The data suggest that new SINE-R retroposons identified in schizophrenia deserve further investigation as potential leads on the understanding of neuropsychiatric diseases.