The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Puccinia iridis on Iris domestica in Korea

범부채에서 녹병균 Puccinia iridis의 동정

  • Choi, In-Young (Division of Agricultural Environment, Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University) ;
  • Kim, Jin-Young (Division of Horticulture Research, Gyeonggi-do Agricultural Research and Extension Services) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
  • 최인영 (전라북도농업기술원 농업환경과) ;
  • 최영준 (군산대학교 생물학과) ;
  • 김진영 (경기도농업기술원 원예연구과) ;
  • 신현동 (고려대학교 환경생태공학부)
  • Received : 2019.02.07
  • Accepted : 2019.03.15
  • Published : 2019.03.01
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Abstract

A rust fungus on Iris domestica (syn. Belamcanda chinensis) from China was previously identified as Puccinia iridis. Accordingly, the identity of the rust fungus on the same host species in Korea needs to be reexamned. Morphological characteristics of the Korean materials matched with P. iridis. Molecular phylogenetic analyses based on internal transcribed spacer and large subunit rDNA sequences of two representative materials confirmed the identification by high sequence similarities of 100% and 99% with the reference sequences available in NCBI GenBank database. Phylogenetic tree inferred from neighbor-joining method proved them to be clustered in P. iridis group. Thus, the rust fungus on I. domestica in Korea was determined as P. iridis. On the other hand, a previous record that Puccinia belamcandae is another rust agent on I. domestica in Korea should be reconfirmed in future.

중국에서 범부채의 녹병균이 Puccinia iridis로 동정됨에 따라 우리나라에서도 범부채의 녹병균을 재검토하였다. 저자들이 채집한 2점의 시료를 형태적으로 검토한 결과 모두 P. iridis의 특징과 일치하였다. 또한 유전분석한 결과 ITS 및 LSU rDNA 영역의 염기서열이 기존에 기록된 P. iridis와 각각 100% 및 99%의 상동성을 나타냈다. 이를 Neighbor-joining 분석법으로 계통수를 작성하였을 때도 P. iridis 계통군에 속하였다. 따라서 우리나라에서 범부채의 녹병균으로 P. iridis의 존재가 확인되었다. 한편, 우리나라에서 2003년에 범부채의 녹병균으로 기록된 Puccinia belamcandae에 대한 재검토는 향후 숙제로 남게 되었다.

Keywords

GNHHDL_2019_v47n1_89_f0001.png 이미지

Fig. 1. Puccinia iridis on Iris domestica. A, Iris domestica plants heavily infected with Puccinia iridis;B, Uredinia formed on the leaf surface; C, Close-up view of erumpent uredinia; D, E, Urediniosporesfocused on the median view (D) and the surface view (E) observed under light microscope; F,Uredinium showing a group of urediniospores; G, H, Urediniospores observed under scanning electronmicroscope.

GNHHDL_2019_v47n1_89_f0002.png 이미지

Fig. 2 Phylogenetic tree inferred from neighbor-joining method using the internal transcribed spacer (ITS) ribosomal DNA (rDNA) region sequences of Puccinia iridis on leopard flower (Iris domestica) isolates and some reference isolates retrieved from GenBank. The numbers above the nodes are the bootstrap values obtained from 1,000 replications. The Korean isolates presented in this study are indicated in bold.

GNHHDL_2019_v47n1_89_f0003.png 이미지

Fig. 3. Phylogenetic tree based on neighbor-joining method comparing the large subunit (LSU) sequences of Puccinia iridis on leopard flower (Iris domestica) isolates and some reference isolates retrieved from GenBank. The numbers above the nodes are the bootstrap values obtained from 1,000 replications. The Korean isolates presented in this study are indicated in bold.

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