• Asian-Australasian Journal of Animal Sciences
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• v.18 no.11
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• pp.1535-1541
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• 2005

Five broiler chicken lines, namely HC, BPB2, CPB2, PB2 and UM1, involving in a selection program and differing in selection intensity and genetic background, were screened for randomly amplified polymorphic DNA (RAPD) polymorphism using 10 selected decamer primers. Nine primers amplified the genomic DNA, generating 200 to 2,500 bp and all detected polymorphism between lines. Out of 74 bands scored using these primers, 34 (50.0%) were found to be polymorphic. The number of polymorphic loci ranged from 3 to 6 with an average of 4.33. Lines differed considerably for within-population genetic similarity estimated by band frequency (WS = 93.55 to 99.25). Between-line genetic similarity estimates based on band sharing as well as on band frequency ranged from 71.35 to 86.45 and from 73.38 to 87.68, respectively. Lines HC and PB2 were the most closely related to the other, while BPB2 and CPB2 appeared to be more distant from each other. The between-line genetic distance based on both band sharing and band frequency revealed the similar trends as for Between-line genetic similarity. Based on BS and BF criteria, BPB2 and CPB2 as well as PB2 and UM1 lines can be merged to launch a new genetic group for further progress in biometrical objectives. A phylogenetic tree, derived using Nei's coefficient of similarity revealed the different pattern of genetic distance between lines.

• The Journal of Korean Medicine
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• v.34 no.1
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• pp.52-68
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• 2013

Objectives: Hyungsang medicine (HM) is an inspection which emphasizes the relationships between pathological patterns and one's facial or bodily shape, color and movement. The purpose of this study was to review previously published study results of the HM. Methods: The authors reviewed a total of 51 HM-related studies published from 2000 to 2012 found on PubMed and various domestic Oriental medicine journals. Each study was classified into theoretical, diagnostic, or treatment-related category. Results: Theoretical studies were mostly based on the Nei-Ching and Dong-Eui-Bo-Gam texts, and dealt with differences in form between types such as masculine and feminine, the five organ image types, the four-type categorization of body essence, vital energy (Qi), mentality, and Blood, Gallbladder and Bladder, the four-type categorization of fish, bird, horse, and turtle types, and the six meridian types. Research on diagnosis has been performed on diagnostic characteristics of HM, correlation between HM and general coordinative manipulation (GCM), and Hyungsang medicine and ante-disease pattern (未病類型). Studies on treatments could largely be classified as treatments for specific diseases using certain acupuncture or herbal prescriptions based on HM. Treatments were mostly evaluated solely through subjective symptom improvement of patients. Conclusions: Our review results suggest that HM-related studies were focused on the constitutional characteristics and clinical utility of HM. To strengthen the theoretical basis of HM and its clinical utility, clinical trials including randomized, treatment-placebo and blind methods are needed.

• Journal of Ecology and Environment
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• v.35 no.1
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• pp.27-34
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• 2012

Typha angustifolia has ecological characteristics of clonal growth similar to Phragmites australis. The plant spreads byclonal growth and seed dispersal. In this study, for the three stands which have different settlement age at the Baksilji wetland in Korea, genetic diversity was estimated by random amplification of polymorphic DNA analysis to evaluate the change in genetic diversity of T. angustifolia during stand development in the same population. Stand (ST) 1 was the oldest and ST 4 was the youngest. ST 5 was in a small ditch out of the Baksilji. Although the ST 1, ST 2, and ST 3 did not differ significantly in vegetational or physical environment, the genetic diversity estimated according to Nei's gene diversity (h) and the Shannon index (i) increased in the order of ST 1 < ST 2 < ST 3 contrary to formative age. The genetic diversity of ST 4 was much higher than that of the other three stands. ST 4 has similar abiotic environmental conditions with slight T. angustifolia dominance, and seems to be in the early establishment stage. ST 5 differed from the other stands in vegetational and soil environments, which can result in stressful cattail conditions. Even though the ST 5 stand was not younger than the ST 4 stand, ST 5 showed the highest genetic diversity. Our results indicate that after early settlement of the T. angustifolia population, genetic diversity within the species decreased over time and that the decreasing pattern of genetic diversity within T. angustifolia stands is not likely to occur under stressful conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.9
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• pp.1234-1239
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• 2006

Murrah and NiliRavi are the important North Indian buffalo breeds occupying the prominent position of being the highest milk producers. These breeds are more or less similar at morphological as well as physiological levels. The technique of RAPD-PCR was applied in the present study to identify a battery of suitable random primers to detect genetic polymorphism, elucidation of the genetic structure and rapid assessment of the differences in the genetic composition of these two breeds. A total of 50 random primers were screened in 24 animals each of Murrah and NiliRavi buffaloes to generate RAPD patterns. Of these, 26 (52%) primers amplified the buffalo genome generating 263 reproducible bands. The number of polymorphic bands for the 26 chosen RAPD primers varied from 3 (OPG 06 and B4) to 26 (OPJ 04) with an average of 10.1 bands per primer and size range of 0.2 to 3.2 kb. DNA was also pooled and analyzed to search for population specific markers. Two breed specific RAPD alleles were observed in each of Murrah (OPA02 and OPG16) and NiliRavi (OPG09) DNA pools. RAPD profiles revealed that 11 (4.2%) bands were common to all the 48 individuals of Murrah and NiliRavi buffaloes. Pair-wise band sharing calculated among the individual animals indicated considerable homogeneity of individuals within the breeds. Within breed, band sharing values were relatively greater than those of interbreed values. The low genetic distance (Nei's) value (0.109) estimated in this study is in accordance with the origin and geographical distribution of these breeds. The RAPD analysis indicated high level of genetic similarity between these two important North Indian buffalo breeds.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.11
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• pp.1519-1523
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• 2006

Through a screening and selection approach method, decamer random markers were used in a technique called random amplified polymorphic DNA (RAPD) assay with 252 genomic DNAs isolated from four major Haimen chicken populations: Rugao (62), Jiangchun (62), Wan-Nan (63) and Cshiqishi (65). A total of 3-score decamer random primers (S241-S260, S1081-S1100 and S1341-S1360) were employed in the preliminary RAPD-polymerase chain reaction (RAPD-PCR) assay with 50 random template DNA samples from all the populations. Four (6.67%) of the primers that produced obvious polymorphic patterns, interpretable and reproducible bands were selected and used with both the individual DNAs from each population and with pooled DNA samples of the four populations in subsequent analyses. The selected primers produced a total of 131 fragments with molecular size ranging from 835 to 4,972 base pairs (bp) when used with the individual DNAs; 105 (80.15%) of these fragments were polymorphic. With the pooled DNAs, 47 stable and characteristic bands with molecular size ranging from 840 to 4,983 bp, of which 23 (48.94%) polymorphic, were also generated. The band-sharing coefficient (BSC) calculated for the individuals in the population and among populations of bulked samples was between 0.8247 (Rugao) and 0.9500 (Cshiqishi); for pairwise populations, it was between 0.7273 (Rugao vs. Wan-Nan) and 0.9367 (Jiangchun vs. Cshiqishi) chicken populations. Using the BSC for individual and pairwise populations, the Nei's standard genetic distances between the chicken populations were determined and ranged from 0.0043 (Jiangchun vs. Cshiqishi) to 0.1375 (Rugao vs. Cshiqishi). The reconstructed dendrogram linked the Jiangchun and Cshiqishi chickens as closely related populations, followed by Wan-Nan, while the Rugao was the most genetically distant among the populations.

• The Journal of Korean Medical History
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• v.30 no.2
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• pp.83-100
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• 2017

Research purpose : Review of the systematized medical theory of Huangdi within the first series of conversations including questions and answers between Leigong and Huangdi. Conclusions : 1. "Huang-di-Nei-Jing" records the 5 theories of Huangdi's system of thinking (黃帝學派). Among these, the contents of the questions and answers with Leigong led to the establishment of the first of Huangdi's treatises. 2. In the questions and answers exchanged between Leigong and Huangdi, the Suwen (素問) Chapter deals with the areas of Yinyang, Zangfu, Cause and Mechanism of diseases, as well as medical ethics. Moreover, the "Lingshu (靈樞)" Chapter discusses distribution of meridian system, routes of circulation of energies, symptoms, acupuncture therapy and diagnosis, etc. 3. Characteristics of the medicine of Leigong and Huangdi can be summarized as follows: Firstly, 12 meridian systems of the "Jingmai (經脈)" section describes the meridian system that ceaselessly circulates in the shape of loops with the inner and outer aspects linked with each other. Secondly, regarding the methods of diagnosis, the "Yinyangleilun (陰陽類論)" section describes the method of diagnosis through palpation of pulse. In addition, the "Wuse (五色)" section describes visual diagnosis that uses five colors. The Wuhang combination of spleen, liver, kidney of "Shicongronglun (示從容論)" section also includes the Wuhang theory of "Jinwenshangshu (今文尙書)". The "Wuse" section discusses areas of the face and five colors, while the "Yinyangleilun" discusses the 'Yinyancixiongmaifa (陰陽雌雄脈法)', the "Jingmai" discusses the 'Yinyancunkoumaifa (人迎寸口脈法)', and the "Zhengsishilun" and "Fangchengshuailun" discuss 'chifuzhenfa (尺膚診法)'. Thirdly, Huangdi's school of thinking did not neglect bencao (本草) and tangye (湯液), which are discussed in the "Jiejingweilun" and "Shicongronglun" sections.

• Proceedings of the KACD Conference
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• 2002.11a
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• pp.721-721
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• 2002

For about half a century. dental implants made of titanium have developed as a method of restoration for the tooth loss. In these days. the titanium implants seem to be considered as the alternative for the conventional prosthodontics. But its hard to say that the titanium implants are superior to the treatments that preserve the natural tooth. As this is a general opinion among dentists. the implant will not be able to be the alternative for all the prosthetic treatments. Clinically, there are many causes for extracting tooth. The severe destruction of the tooth structure or periodontal diseases leads to inevitable tooth extraction. When the complete cure is doubtful because of narrow intraoral visibility and improper accessibility in approaching to the tooth and periodontal lesion, we. clinicians often inevitably extract tooth. Passive treatments like conventional restoration, curettage or surgical flap cant be the perfect treatments for the tooth that has subgingival root caries or severe periodontal diseases involved furcation. Many clinicians might have been forced to pull out the relatively healthy tooth by the difficulties of approaching to the lesions and poor prognosis. Though the intentional tooth replantation is performed sometimes. as it doesnt have enough scientific foundation. it has not been considered as a popular treatment method yet. I have been felt keenly the necessity of positive tooth preservation, so I have been attempting the treatment that has new concept. calling Natural Tooth Implantation (NTI) clinically. NTI differs from the tooth replantation in the goal for the treatment and biological healing process. Now. I confirm that NT! is a very positive and valid method of tooth preservation. Like you can get from the name. NTI is the dental implant procedure using natural teeth and similar to the healing process of the titanium implants in many aspects. I have been using biocompatible composite resin. DRM. with NTI and got affirmative clinical results from that. So I would like to introduce.roduce.

• International Journal of Industrial Entomology and Biomaterials
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• v.19 no.2
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• pp.249-253
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• 2009

Raily ecorace of Indian tasar silkworm is wild in nature and distributed abundantly in dense deciduous forest on Shorea robusta (Sal) in Bastar ($17^{\circ}4'$ and $20^{\circ}34'$ N, $80^{\circ}15'$ and $82^{\circ}15'$ E and altitude ranging from 150 to 1200 mMSL) forest ranges of Chhattisgarh, India. It is represented by about 20 populations. Out of those, eleven populations showed intra- as well as inter- population variability based on phenotypic expression and also in major economic traits viz. cocoon weight, shell weight, filament length and denier. Genetic diversity in these eleven populations was studied using Inter-Simple Sequence Repeat (ISSR) markers. The band profiles generated with eight ISSR primers have depicted variation in band size. All the primers exhibited polymorphism which is an indicative of the genetic variation in individual Raily silkworm. Among the populations, total polymorphism recorded was 76%. The population genetic aspects assessed through POPGENE software package are discussed in the paper. Nei's gene diversity (h) ranged from 0.194 to 0.337 exhibiting high heterozygosity. Relevance of the present study is of high significance in formulating conservation strategies and sustainable utilization of the economically important Raily ecorace of Antheraea mylitta.

• Journal of Korean Society of Forest Science
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• v.97 no.2
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• pp.187-195
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• 2008

Picea jezoensis (Sieb. et Zucc.) Carriere is one of the major and widespread components of the cold-temperate and boreal forests in Russian Far East, northeast China, Korea, and Japan. However, it is restricted to a highly fragmented range in South Korea with small populations. Mean expected heterozygosity $(H_e)$ based on 22 loci in 11 isozyme systems was 0.077 for four sampled populations that covered the whole distribution range of P. jezoensis in South Korea. This value is within the range reported for conifers, but it is very low compared to that of other spruce species as well as that of P. jezoensis populations in Russian Far East. Most populations had a slight excess of heterozygotes and the Wright's $F_{IS}$ (-0.019) was comparable to that previously reported for other spruce species. In all of the four populations, the Wilcoxon sign-rank test indicated no greater heterozygosity than that expected for populations at mutation-drift equilibrium, suggesting that the populations have not been bottlenecked recently. Despite a fragmented range and isolated populations, population differentiation was not high $(F_{ST}=0.047)$ and the number of migrants per generation was 5.09. Nei's genetic distances were also small $({\bar{D}}=0.005)$ but strongly related to geographic distances between populations, suggesting an Isolation by Distance. The northernmost isolate, Mt. Gyebang population was genetically distinct from the other three populations. Implications for the conservation of genetic variation of P. jezoensis in South Korea were discussed.

• Korean Journal of Environmental Biology
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• v.22 no.2
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• pp.295-299
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• 2004

The lacertid lizard Takydromus wolteri widely distributed in South Korea, but intraspecific diversity of this species was not almost studied. T. wolteri represented by 8 specimens collected from 5 localities were analyzed by the Randomly Amplified Polymorphic DNA (RAPD) method with 28 random decamer primers and the phenogram constructed by using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method based on RAPD data. The populations of T. wolteri using in this paper were collected from Gyeonggi-do, Chungcheongbuk-do, Jeju-do, Jeollanam-do and Gyeongsangnam -do in South Korea. Among 68 different bands detected in RAPD analysis, 59 bands (87％) showed polymorphism. The phenogram showed that the populations of T. wolteri were grouped into two. These results suggest that T. wolteri were supported intraspecific diversity.