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Antibacterial Activity of Ethanol Extract of Root Bark of Morus alba against Selected Fish Pathogenic Bacteria (일부 어병 세균에 대한 상백피 에탄올 추출물의 항균활성)

  • 목종수;송기철;최낙중;양호식
    • Journal of Aquaculture
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    • v.14 no.4
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    • pp.221-226
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    • 2001
  • The ethanol extract of root bark of Morus alba strongly inhibited the Gram positive bacteria like Streptococcus sp., Lactococcus garvieae and Staphylococcus sp., but weakly the Gram negative bacteria like Listonella anguillarm and Edwardsiella tarda. It was more effective in liquid medium than in solid medium. The minimum inhibition concentration (MIC) of the extract in liquid medium was 19.8 and 790~1185 $\mu\textrm{g}$/$m\ell$ for the Gram positive and Gram negative bacteria, respectively. The extract concentration, at which the growth was totally inhibited, was 67.2~403.0 $\mu\textrm{g}$/$m\ell$ for the Gram positive bacteria but it was as high as 1185 $\mu\textrm{g}$/$m\ell$ for L. anguillarum and almost ineffective against E. tarda. For diet supplementation of the extract, effective soaking duration was 3 minutes. The fish diet soaked in the extract inhibited the growth of all the tested Gram positive strains, but not the Gram negative strains. The relationship between the weight of fish diet and absorption of the extract by the fish diet was Y=7.5757X + 4.6962($R^2$ = 0.9998).

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Identification of Positive and Negative Regulatory Elements of the Human Cytochrome P4501A2 (CYP1A2) Gene

  • Chung, Injae;Jeong, Choonsik;Jung, Kihwa;Bresnick, Edward
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1997.04a
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    • pp.81-81
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    • 1997
  • We previously demonstrated an enhancer-like positive regulatory element within a 259-bp sequence (-2352 to-2094 bp) of the human CYP1A2 gene in HepG2 cells. Three protein binding sites were identified by DNase I footprint analyses within the 259-bp sequence: protected region A PRA ( -2283 to-2243 bp), PRB (-2218 to-2187 bp), and PRC (-2124 to-2098 bp) (I. Chung and E. Bresnick, Mol. Pharmacol. 47, 677-685, 1995). In the present study, the functional significance of those protected regions was examined. Transfection experiments with deletion and substitution mutants defined the PRB and PRC as containing positive and negative regulatory elements, respectively. Human breast carcinoma MCF-7 cells were cotransfected with a hepatocyte nuclear factor-1 (HNF-1) expression vector and CYP1A2 promoter-or thymidine kinase promoter-luciferase remoter gene constructs. HNF-1, which contributes to the liver specificity of genes, enhanced reporter gene activity in a PRC sequence-dependent manner. These results suggested that PRC could exist bound to a repressor which was displaceable by other transcription factors such as HNF-1. Results obtained by transfection of HepG2 hepatoma cells with various PRB substitution mutant-luciferase gene fusion constructs indicated that the entire sequence of PRB was necessary for promoter activity. Consequently, the regulation of CYP1A3 expression is very complex, requiring a number of both positive and negative regulatory factors.

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A Case of Cold Agglutinin Hemolytic Anemia Complicating Mycoplasma pneumoniae pneumonia in Children (소아에서 Mycoplasma pneumoniae pneumonia에 합병된 한냉응집소 용혈성 빈혈 1례)

  • Jo, Sung Ok;Park, Hyeon Jin
    • Pediatric Infection and Vaccine
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    • v.5 no.2
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    • pp.302-307
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    • 1998
  • Hemolytic anemia due to cold agglutinin disease is a known complication of Mycoplasma pneumoniae infection but is rarely observed, particularly in children. A case of Mycoplasma pneumonia complicated with hemolytic anemia is presented. A 7 year-old girl was adimitted because of fever, cough, sputum and pale appearance. Chest X-ray showed pneumonic consolidation of Rt. upper lobe, lingular division. Laboratory studies disclosed the following values : Hb 5.3g/dL, Hct 11.1%, reticulocyte 2.9%, indirect Coombs test negative, direct Coombs test(monovalent) Anti-C3d positive, Anti-IgG negative, Anti-IgM negative, cold agglutinin titer 1 : 256, mycoplasma antibody titer 1 : 640, total bilirubin 1.0mg/dL. Initial PBS before wanning showed agglutination of red blood cells. The diagnosis of cold agglutinin hemolytic anemia complicating mycoplasma pneumonia was made. And treatment with roxithromycin, prednisolone and avoiding cold exposure was initiated, and complete recovery ensued. We report a case of cold agglutinin hemolytic anemia complicating mycoplasma pneumonia in children.

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The Mediating Effect of Humor on the Relationship between Perceived Stress and Depression in College Students (대학생의 지각된 스트레스와 우울의 관계에서 유머의 매개효과)

  • Hong, Eunyoung
    • The Journal of Korean Academic Society of Nursing Education
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    • v.20 no.4
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    • pp.558-568
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    • 2014
  • Purpose: This study examines the relationships among perceived stress, humor style, responding humor and depression among college students and the mediating effect of humor between perceived stress and depression. Method: During the period May 6-16, 2013, a convenience sample of 200 subjects was obtained from college K in city G. Data analysis was performed by Pearson's correlation coefficients and regression analysis. Results: The average BDI score was 7.7. There were 40 (20.0%), 16 (8.0%), 8 (4.0%), and 11 (9.0%) students with mild, moderate, and severe depression, respectively. The group with depressive symptoms constituted 3.5% of students. There was a significant positive correlation between negative stress perception and depression. Conversely, there was a significant negative correlation between adaptive humor style and negative stress perception. Ultimately, there were significant negative correlations between positive stress perception, adaptive humor style, maladaptive humor style, responding humor and depression. To test the mediating effect of adaptive humor style in the relationship between negative stress perception and depression, a Sobel test was performed and Z-score of 1.7736 (p=.076) obtained. Conclusion: Though the mediating effect of an adaptive humor style on the relationship between perceived stress and depression was not significant, considering the negative correlation with depression, strategies to increase an adaptive humor style and responding humor is recommended to prevent depression in college students.

THE STRUCTURE OF THE REGULAR LEVEL SETS

  • Hwang, Seung-Su
    • Bulletin of the Korean Mathematical Society
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    • v.48 no.6
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    • pp.1245-1252
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    • 2011
  • Consider the $L^2$-adjoint $s_g^{'*}$ of the linearization of the scalar curvature $s_g$. If ker $s_g^{'*}{\neq}0$ on an n-dimensional compact manifold, it is well known that the scalar curvature $s_g$ is a non-negative constant. In this paper, we study the structure of the level set ${\varphi}^{-1}$(0) and find the behavior of Ricci tensor when ker $s_g^{'*}{\neq}0$ with $s_g$ > 0. Also for a nontrivial solution (g, f) of $z=s_g^{'*}(f)$ on an n-dimensional compact manifold, we analyze the structure of the regular level set $f^{-1}$(-1). These results give a good understanding of the given manifolds.

NEGATIVE SOLUTION FOR THE SYSTEM OF THE NONLINEAR WAVE EQUATIONS WITH CRITICAL GROWTH

  • Jung, Tacksun;Choi, Q.-Heung
    • Korean Journal of Mathematics
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    • v.16 no.1
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    • pp.41-49
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    • 2008
  • We show the existence of a negative solution for the system of the following nonlinear wave equations with critical growth, under Dirichlet boundary condition and periodic condition $$u_{tt}-u_{xx}=au+b{\upsilon}+\frac{2{\alpha}}{{\alpha}+{\beta}}u_+^{\alpha-1}{\upsilon}_+^{\beta}+s{\phi}_{00}+f,\\{\upsilon}_{tt}-{\upsilon}_{xx}=cu+d{\upsilon}+\frac{2{\alpha}}{{\alpha}+{\beta}}u_+^{\alpha}{\upsilon}_+^{{\beta}-1}+t{\phi}_{00}+g,$$ where ${\alpha},{\beta}>1$ are real constants, $u_+={\max}\{u,0\},\;s,\;t{\in}R,\;{\phi}_{00}$ is the eigenfunction corresponding to the positive eigenvalue ${\lambda}_{00}$ of the wave operator and f, g are ${\pi}$-periodic, even in x and t and bounded functions.

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Microbacterium esteraromaticum CS3-1의 toluene 분해능에 미치는 benzene, ethylbenzene, xylene의 영향

  • Jeon, Yeon-Sin;Lee, Eun-Yeong;Jo, Gyeong-Suk;Ryu, Hui-Uk
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.179-182
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    • 2000
  • Toluene-degrading bacterium, Microbacterium esteraromaticum CS3-1 was isolated from the biofilter for the removal of BTEX. Microbacterium esteraromaticum CS3-1 was shown to utilize toluene as a primary carbon and energy source. Effect of mixed BTEX gases on toluene degradation rate by M. esteraromaticum CS3-1 was investigated in this study. Toluene degradation rate was 2.26(only toluene), 2.06(toluene+benzene), 2.57(toluene+ethylbenzene), and 4.74(toluene+xylene) mmole $toluene\;{\cdot}\;g-DCW^{-1}\;{\cdot}\;h^{-1}$. Toluene degradation rate was 2.26(only toluene), 1.23(toluene+benzene+ethylbenzene), 1.52 (toluene+ethylbenzene+xylene), and 1.76(toluene+benzene+ethylbenzene+xylene) mmole $toluene\;{\cdot}\;g-DCW^{-1}\;{\cdot}\;h^{-1}$. The presence of BTEX compounds over three mixtures had a negative effect on toluene degradation rate. Toluene degradation rates were enhanced by the presence of ethylbenzene or xylene, whereas the presence of benzene had a negative effect on toluene degradation rate in comparison with toluene degradation rate when only toluene is existent.

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Effect of Attitude towards Parent Brand on Extended Fashion Product in Fashion Brand Extension (패션브랜드 확장시 모브랜드에 대한 태도가 확장패션제품에 미치는 영향에 관한 연구)

  • Rhee, Young-Ju
    • Fashion & Textile Research Journal
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    • v.10 no.4
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    • pp.506-514
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    • 2008
  • The purpose of this study was to investigate the effect of attitude towards parent brand on extended fashion products in case of fashion brand extension. The subjects of this study were 386 college students and data were collected using survey questionnaire. For data analysis, factor analysis and path analysis were applied. The results showed that there were four factors (e.g., familiarity attitude, emotional attitude, value-expressive attitude, utility information attitude) in attitude towards parent brand and six factors (e.g., economical risk, utilitarian risk, social risk, psychological risk, time/advantage loss risk, risk for losing opportunity) under perceived risk in extended product. The results showed that attitude towards parent brand had a negative effect on perceived risk in extended products and had a positive effect on attitude towards extended products. Perceived risk in extended products then had a negative effect on purchase intention of extended products whereas attitude towards extended products had a positive effect on purchase intention of extended products.

Effect of Magnetic Field on Negative DC Corona Discharge (부극성 직류 코로나 방전에 미치는 자계의 영향)

  • Park, J.Y.;Kim, I.K.;Jung, J.G.;Kim, G.H.;Kim, J.D.;Koh, H.S.
    • Proceedings of the KIEE Conference
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    • 1999.07e
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    • pp.2308-2310
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    • 1999
  • The effect of magnetic field on NOx removal characteristics from a combustion flue gas has been experimentally investigated for a DC corona discharge reactor with a multipoint plate geometry in our Lab. Combustion flue gas removal efficiency of this type was much higher than that of the other types. In this paper, needle-plate geometry discharge system with applied magnetic field at a right angle to the electric field was made. Input power source was used negative DC. The corona discharge characteristics for this system was investigated and compared with the corona characteristics for the corona discharge system without magnetic field.

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One case of Trigeminal neuralgia (三차神經痛 患者의 治驗 1例)

  • Kim Hong-Jin;Kim Jong-Han;Choi Jung-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.14 no.2
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    • pp.295-302
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    • 2001
  • Trigeminal neuralgia has a specific property which burning pain emerges suddenly and spasmodically. The following pain continuously emerges during 20 seconds or 30 seconds and then the pain disappears. This process of the pain emerges repeatedly. Trigeminal neuralgia was called Myuntong(麵痛). We experienced a patient who had Trigeminal neuralgia for five years. she was successfully treated by the herb-medication, accupunture and negative. The medication taken by the patient were Seunghyungsan(升荊散) and Jowyuseung chengtang(謂胃升淸湯) and so on. The accupunture was mainly done at mainly done G-14(陽白), S-2(瀉白), G-3(上關), S-36(足三里), LI-4(합閤穀) and so on. The negative was done sternocleidomastoid muscle, trapezius and Masseter muscle.

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