• Journal of Ginseng Research
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• v.46 no.6
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• pp.731-737
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• 2022

Background: The number of primary human immunodeficiency virus (HIV)-1 non-B subtype infections (non-B) and that of reports regarding the differences in the pathogenesis of subtype B and non-B infections are increasing. However, to the best of our knowledge, there have been no reports on gross deletion in the nef gene (g∆nef) in non-B infections. Methods: To determine whether there is a difference in the change in CD4⁺ T cells after treatment with Korean Red Ginseng (KRG) between patients with subtype B and non-B infections, we retrospectively analyzed and compared the annual decrease in CD4⁺ T cells (AD) and the proportion of g∆nef in 77 patients who were followed for more than 10 years in the absence of combination antiretroviral therapy. Results: Overall, AD was significantly faster in patients with non-B infections than in those with subtype B infections. Survival analysis showed that the survival probability was significantly higher in subtype B than in non B-infected patients. These differences mainly resulted from significant differences in the amount of KRG and age. In the patients treated with KRG, there was a significant correlation between the amount of KRG and the AD in both subtypes. Interestingly, there was a significant correlation between the amount of KRG and the proportion of g∆nef in patients infected with subtype B, but not in those infected with non-B. The same phenomenon was observed when the KRG dose was adjusted. Conclusion: Our results suggest that non-B may be biologically more stable than subtype B.

• Proceedings of the Ginseng society Conference
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• 2002.10a
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• pp.185-211
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• 2002

We have found many beneficial effects of the long-tenn intake of Korean red ginseng (KRG) in human immunodeficiency virus (HIV) type-I infected patients, including the maintenance of CD4+ T cell count for 10 years with KRG only and the delayed development of resistance mutation to ZDV. In this study, to investigate whether KRG-intake could affect the clinical progression and nef gene variation, we determined 200nef sequences from 70 patients. Follow-up period was $8.8{\pm}2.9$ years and annual decrease in CD4+T cell was $41{\pm}57/ul.$ Nested polymerase chain reaction (PCR) and direct sequencing were perfonned with peripheral blood mononuclear cells (PBMC) obtained at times during the study period. First, there was a significant correlation between survival duration and duration of KRG-intake $(36.8{\pm}38$ months)(P=0.000). There were significant correlations between the last NefProg score and CD4+ T cell count (r= 0.208, P<0.05) and annual decrease in CD4+ T cell count (r =0.346, P<0.01) in 70 patients. In addition, there were significant correlations between KRG-intake and annual decrease (r= 0.323, P<0.01), and the CD4+ T cell count itself (r=0.229, p<0.05). Furthennore, there was also a mild significance between the NefProg score and the duration of KRG-intake in only SP and RP (n=30, r=-0.281, P=0.067). In addition, we detected various defects in 21 patients $(30.0\%),$ not including 5 premature stop codons. Ten $(12.5\%)$ patients showed repeated deletion of an amino acid. Four of 10 patients were gross deletions and they were treated with KRG for more than 20 months. The number of patients with repeated gross deletions was significantly higher in the order of slow progressors $(18\%)$, typical progressors($3\%$), and rapid progressors($0\%$) (P<0.05). We also observed that long-tenn intake of KRG might make the change from A or D to T at position 54 and decrease NefProg score. Taken together, our results show clear evidence that the long-term intake of KRG has effects on nef gene variation as well as definite clinical usefulness.

• Journal of Microbiology
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• v.41 no.3
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• pp.232-238
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• 2003

Previous phylogenetic studies on human immunodeficiency virus type 1 (HIV-1) isolated from Korean patients suggest that the major subtype of Korean isolate is subtype B. In this subtype, some of the Korean isolates seem to be clustered exclusively of foreign isolates. Presence of this so-called “Korean clade” among Korean isolates is unique but needs verification since the number of Korean isolates used in previous studies was limited. This study aimed to identify the presence of the “Korean clade” by molecular phylogenetic analysis using all the Korean nef gene sequences registered in the NCBI GenBank (N=243) together with 32 reference strains and 77 foreign isolates. Extensive analysis of the nef gene nucleotide sequences by neighbor-joining method revealed the following. Most (83.1 ％) of the Korean isolates belonged to subtype B, and 81.2％ of subtype B were clustered together and excluded foreign isolates (bootstrap value=91.9％ ). Within Korean subtype B cluster, no characteristic subcluster formation was evident since the bootstrap values for the subcluster were very low. Due to limited information, the phylogenetic analysis failed to identify the epidemiological linkage among specific groups such as homosexuals and hemophiliacs within the Korean subtype B cluster. Detailed analysis and epidemiological information are needed to clarify the origin and significance of the Korean subtype B cluster.

• Architectural research
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• v.4 no.1
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• pp.25-31
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• 2002

For more than a hundred years, art and architectural historians, architects, and engineers interested in structure have attempted to interpret Gothic architecture, one of the most technologically complex and sophisticated structural systems in history. Indigenous Gothic, however, such as non-vaulted Gothic in the Lower Languedoc region of southern France, has been largely ignored. This study intends to analyze the Gothic non-vaulted nef unique (aisleless) structures of Lower Languedoc which have never been scientifically tested, and to provide as comprehensive an explanation as possible of the way in which these non-vaulted buildings work. In order to achieve this goal, this paper is to examine, by means of finite element computer analysis, a selected example of an existing building. The church of Notre-Dame de Lamourguier, the earliest surviving example of a Gothic nef unique with wide-span diaphragm arches in Lower Languedoc, is selected. Thus, hypothetical models of diaphragm arch buildings and an existing building as a complete structural system were scientifically analyzed in order to provide a comprehensive explanation of how the non-vaulted nef unique system works. The result of the analysis, allows us better to understand the structural behavior of this type of masonry arcuated system and the processes involved in the design and construction of medieval buildings.

• Journal of applied mathematics & informatics
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• v.19 no.1_2
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• pp.427-438
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• 2005

From a notion of d-pseudo-orthogonality for a sequence of poly-nomials ($d\;\in\;{2,3,\cdots}$), this paper introduces three different characterizations of natural exponential families (NEF's) with polynomial variance functions of exact degree 2d-1. These results provide extended versions of the Meixner (1934), Shanbhag (1972, 1979) and Feinsilver (1986) characterization results of quadratic NEF's based on classical orthogonal polynomials. Some news sets of polynomials with (2d-1)-term recurrence relation are then pointed out and we completely illustrate the cases associated to the families of positive stable distributions.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.54-54
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• 2021

The Melanoma Research Coalition reported melanoma affects humans of various races. This study was conducted to confirm the inhibitory effect of melanogenesis in B16 F10 cells of Nypa fruticans Wurmb of ethyl acetate fraction (NEF). Nypa fruticans Wurmb is an important component of the East Asian mangrove vegetation. It belongs to Araceae family. Traditionally, N. fruticans was used to treat various diseases such as asthma, sore throat, liver disease, a pain reliever, and can also be used as sedative and carminative. The present study, the inhibitory effect on melanogenesis was determined by Western blotting and RT-qPCR. The level of expression of tyrosinase, TRP-1, and TRP-2 is regulated by microphthalmia-associated transcription factor (MITF) and cAMP, and cAMP affects the activity of protein kinase A (PKA). Activated PKA stimulates the phosphorylation of cAMP-reactive element-binding protein (CREB) in the nucleus, thereby increasing the amount of MITF expression and enhancing melanogenesis. Western blotting and RT-qPCR analysis showed that NEF treatment decreased the expression of tyrosinase. Similarly, TRP-1 and TRP-2 levels were decreased, which were decreased significantly at compared with the untreated control. Also, NEF attenuated the IBMX mediated increase in the intracellular cAMP level and the phosphorylation of PKA. In conclusion, NEF significantly inhibited the expressions of melanogenesis through cAMP/PKA/CREB signaling pathways.

• Architectural research
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• v.2 no.1
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• pp.1-6
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• 2000

Ever since Viollet-le-Due began to examine Gothic structural elements using his method of geometrical analysis in the nineteenth century, art and architectural historians and a few engineers have periodically attempted to ascertain the structural advantages of the various characteristic features of Gothic architecture. In none of these studies, however, has the way forces work within the lightweight and spacious masonry Gothic buildings been precisely interpreted. The approach taken by art and architectural historians has therefore tended to be primarily descriptive and to be based on intuitive assumptions. This study intend to analyze the Gothic non-vaulted nef unique(aisleless) structures of Lower Languedoc which has never been scientifically tested, and to provide as comprehensive an explanation as possible of the way in which these non-vaulted buildings work. In order to achieve this goal, this paper Is to examine, by means of finite element analysis. the links between the width of non-vaulted aisleless structures, the configuration of the arches, diaphragm arch, and the buttress. Finite element analysis with a computer provides a more accurate analysis than the methods of analysis that have been heretofore applied to Gothic structures, as well as permits us to visualize the global stress behavior of the structure. Combined with traditional methods of studying historical buildings, therefore, finite element analysis inevitably give us a broader understanding of the processes involved in the design and construction of medieval buildings.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.77-77
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• 1995

Human immunodeficiency virus type 1 (HIV-1) contains several nonstructural genes which are required for the viral replication and disease pathogenesis. Among them, tat and nef genes encode an essential transactivator of HIV-1 LTR and a pluripotent protein which seems to be essential for the in vivo but not in vitro viral replication, respectively. We constructed two tat and n of defective HIV-1 and tested for their ability to replicate in several T cells. The defective viruses did not replicate in CD4$\^$+/ T cells, but rescued in the recombinant Jurkat-tat cell which also contains tat gene. The replication of tat and nef defective HIV-1 which expresses chloramphenicol acetyltransferase(CAT) gene was easily detected by a sensitive CAT assay. No revertant was identified during the passages of the mutant viruses for more than two months in Jurkat-tat cells. tat and n of defective HIV-1 could be used instead of wild type viruse for several purposes such as inhibitor screening and development of attenuated AIDS vaccine.

• Proceedings of the Korean Society of Computer Information Conference
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• 2009.01a
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• pp.89-92
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• 2009

최근 일반인들에게 널리 보급되고 있는 DSLR 디지털 카메라로 촬영되는 정지 이미지는 범용의 JPEG 포맷이나 제조사별 독자적인 RAW 파일 포맷으로 저장매체에 저장된다. 이와 함께 PMP 등의 휴대용 멀티미디어 기기를 활용하여 촬영된 RAW 파일 포맷을 재생 및 확인하기 위한 요구가 증대되고 있지만, RAW 파일 포맷이 디지털 카메라 제조사별로 다르고, 해당 포맷에 대한 구체적인 정보가 공개되지 않는 이유로 대부분의 휴대용 멀티미디어 기기에서 RAW 파일 재생 기능을 지원하지 못하고 있는 실정이다. 본 연구에서는 RAW 파일 포맷을 분석하여 향후 임베디드 멀티미디어 기기에서 모든 RAW 파일 포맷을 지원할 수 있는 라이브러리 구축을 위한 사전 작업을 수행하였다. Cannon의 CRW, CR2 포맷과 Nikon의 NEF 포맷을 분석한 결과를 설명한다. 분석 결과를 바탕으로 RAW 파일 포맷에서 JPEG 파일음 성공적으로 추출하였다. MRW, PEF, DCR, DNG 등의 RAW 파일 포맷을 추가로 분석하여 모든 RAW 파일을 재생한 수 있는 임베디드 장비 및 PC용 RAW 파일 재생 라이브러리를 구축할 것이다.

• Magazine of the Korean Society of Agricultural Engineers
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• v.45 no.3
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• pp.65-72
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• 2003

The objective of this study is to evaluate the stability of the design criteria recommended by Betram and Terzaghi as compared with the experimental result. A series of NEF tests was conducted to determine the loss of volume in base soils. The three kinds of base soils classified as CL, SC and SM are used for the NEF tests with various hydraulic pressures and filters following upper and lower bond of the criteria. Volume loss characteristics of the base soils was examined closely by the results of the test successfully. Firstly, it was found that the loss of base soils was mostly eroded at the first stage of seepage. Secondly, the amount of loss volume was ranked CL > SM > SC in order of their amounts for upper criteria, and SM > CL > SC orderly for lower criteria. Thirdly, the volume loss of all soils was increased with increasing the hydraulic pressures. And lastly, the needs of the new design criteria was proved for the control of seepage and piping.