• 한국산림과학회지
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• 제101권1호
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• pp.62-68
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• 2012

우리나라 계방산, 덕유산, 지리산에 분포하고 있는 가문비나무 집단의 보존과 복원 전략 개발에 요구되는 생육 환경을 구명하기 위하여, 가문비나무 침엽의 수명, 침엽 내 엽록소 및 질소 배분 양상을 조사하였다. 가문비나무의 평균 침엽 생존율은 계방산(87.%)이 가장 높았고, 지리산(71.6%)이 가장 낮았으며, 침엽의 생존율은 침엽 연령 증가와 함께 감소하였다. 덕유산과 지리산 가문비나무의 침엽은 연령이 증가함에 따라 엽록소 a, b 모두 증가하였으나, 계방산 가문비나무의 엽록소 함량은 2년생 침엽에서 가장 높았다. 카로테노이드 함량은 계방산 가문비나무 침엽에서 가장 높았으며, 침엽의 연령이 증가함에 따라 카로테노이드 함량은 증가하였다. 엽록소 a와 b의 비는 지리산에서 가장 높았고, 계방산에서 가장 낮게 나타났다. 세 집단의 침엽 연령이 증가함에 따라 엽록소와 카로테노이드 함량의 비는 점차 감소하였다. 가문비나무 침엽 내 질소 함량은 덕유산이 1.51%로 가장 높게 나타났으며, 계방산이 1.40% 으로 가장 낮았고, 질소 함량은 침엽 연령이 증가하면서 감소하였다. 엽록소와 질소 함량의 비는 침엽의 연령이 증가함에 따라 증가하였다.

• 대한임상검사과학회지
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• 제41권4호
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• pp.189-195
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• 2009

Lung cancer is a type of cancer with high mortality; its 5-year survival rate is at a low 14%. Related cytological tests include sputum, bronchial brushing, bronchial washing and fine needle aspiration cytology test etc. From the test specimens in which sputum, bronchial brushing, bronchial washing, and fine needle aspiration cytology were performed, the sensitivity, specificity and accuracy between cytology test and histology test. In the sputum test, sensitivity was 27.71% and specificity was 98.02%, and the bronchial brushing test showed sensitivity of 93.33% and a specificity of 91.3%. The bronchial washing test was a sensitivity of 53.7% and its specificity was 98.9%, and the fine needle aspiration cytology test showed sensitivity and specificity were 88.46% and 72.97%, respectively. In the specimens diagnosed as normal at the sputum test, malignant diagnosis was found in 21 specimens of bronchial brushing, 30 cases of bronchial washings and 37 cases of fine needle aspiration cytology specimens. In the specimens diagnosed as normal at the bronchial washing test, malignant diagnosis was found in 5 specimens of sputum, 7 specimens of bronchial brushin and 1 cases of fine needle aspiration cytology. One specimens found to be normal in fine needle aspiration cytology turned out to maligant in sputum test. The result of this research shows that, in diagnosis lung cancer, a test method of high sensitivity and specificity should be pursued. However, depending on the location and malignancy of the illness, diagnosis may not be obtained in some cases. Therefore, we conclude that the cytological tests performed for lung cancer testing such as sputum, bronchial brushing, bronchial washing, and fine needle aspiration cytology should be carried out in a mutually complementary manner.

• 임산에너지
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• 제23권1호
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• pp.19-25
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• 2004

본 연구의 목적은 현재 산림쇠퇴 징후가 나타나고 있는 반월공단지역에 식재되어 있는 잣나무와 리기다소나무의 생장저하 실태와 광합성 특성을 밝히는데 있다. 이를 위해 안산시 전망대공원에 피해구를, 경기도 광주시에 소재하고 있는 경희대학교 연습림에 대조구를 선정하여 인공식재된 약 10년생의 잣나무와 리기다소나무 2수종을 비교하였다. 잣나무 및 리기다소나무 2수종 모두 피해구에서 생장량이 감소하였으며, 침엽의 잔존율도 저하하였다. 또한 2수종 모두 피해구에서 대조구에 비하여 순광합성속도가 저하하였고, 광-광합성곡선의 초기기울기인 양자수율도 저하하였다. 클로로필 a+b, b의 함량도 피해구에서 감소하였으며, 이 결과는 침엽의 연령이 증가함에 따라서 집광운반능력이 낮아져 침엽의 잔존율이 낮아지는 결과를 초래하였다고 생각된다. 이상의 결과로부터 최근 대기오염이 심한 반월공단지역에서 생육하고 있는 잣나무 및 리기다소나무의 생장감소 원인은 침엽의 클로로필 함량이 광화학계의 활성을 저하시켜 광합성 능력이 감소되어 최종적으로 생장감소를 초래한 것으로 판단된다.

• 한방재활의학과학회지
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• 제19권1호
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• pp.187-199
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• 2009

Objectives : The electroacupuncture was generally has been used in oriental medicine doctors. In recent years, there have been a few studies about safety and stability of acupuncture needle in itself, but then again research of acupuncture needle safety and stability on applying electroacupuncture have been insignificant. Therefore we investigated the safety and change in mechanical characteristic of acupuncture needle in electroacupuncture therapy. Methods : We observed mechanical characteristic change by SEM(Scanning Electron Microscope) and EDX(Energy Dispersive X-ray Spectroscopy), evaluated the hardness by vickers hardness tester. We used MTT assay and cell stain to study about biocompatibility of electroacupuncture. Results : In this study, any corrosion of material, alternation of elements, and change of hardness were not observed in surface analysis using SEM and EDX. In cytotoxity evaluation using MTT assay and cell stain, cell survival rate was low when practicing the electroacupuncture for more than 3 hours. Conclusions : Change of mechanical property was not observed based on the test results using surface analysis and hardness estimation by the electroacupuncture. And considering the biocompatibility, electroacupuncture was thought to be safe in an hour based on cytotoxity evaluation using MTT assay and cell stain.

• 한국환경생태학회지
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• 제7권1호
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• pp.10-21
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• 1993

소나무, 곰솔, 젓나무 및 독일가문비를 대상으로 인위적으로 산도를 조절한 인공산성우(PH 3.0, 4.0 및 5.0)와 지하수(pH 6.5)를 1991년 4월부터 1993년 8월까지, 생육기간 중에는 주 2~3회, 겨울철은 주 1회 1회 5mm의 강도로 처리하였다. 인공산성우를 처리하면서 수종별 발아 및 득묘율, 토양산도, 유묘의 생장 및 접촉각(contact angle)을 조사.비교하여 얻은 결과는 다음과 같다. 묘목의 생장관련 형질(묘고, 지하부, 엽량 및 개체당 건중량)의 경우에는 산성우처리 초기에는 산성우 처리가 양료로서의 효과를 가지는 것으로 판단되며, 수종별로 상이한 반응을 보였다. 그러나 처리가 2년 이상 지속된 3차년도에서는 처리산성우의 산도수준이 높을수록 모든 수종에서 생장관련 형질들이 감소되는 경향을 보였으며, 이는 토양완충력의 한계 이상으로 투하되는 산집적에 의한 결과로 판단된다. 엽피해율이나 엽량(낙엽율)등의 자료는 처리산성우의 산도수준과 관련지어져 있는 것으로 나타나 산성우 피해의 조기판단 기준으로 사용가치가 높을 것으로 판단된다. 토양산도는 처리간 고도의 통계적 유의차가 인정되었다. 침엽의 표면과 물방울이 이루는 접촉각을 측정한 결과, 처리산성우의 산도수준이 높을수록 엽령이 높을수록 접촉각의 크기는 낮아졌다. 접촉각을 측정비교하는 방법은 야외에서 침엽수류에 대한 산성우 피해의 조기판단을 위한 알맞고 훌륭한 기준이라 판단된다.

• Clinical and Experimental Reproductive Medicine
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• 제30권3호
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• pp.241-248
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• 2003

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

• 한국산림과학회지
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• 제64권1호
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• pp.47-51
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• 1984

미국(美國) 남부(南部)소나무류(類) 중(中)에서 Pinus rigida, Pinus taeda, Pinus serotina 세 수종간(樹種間)의 교잡종(交雜種)들의 개발(開發) 가능성(可能性)을 검토(檢討)하기 위하여 전북지역(全北地域)에 식재(植栽)된 13년생(年生) 시험림(試驗林)을 대상(對象)으로 하여 생장(生長)과 몇가지 형질(形質)을 조사(調査)한 결과(結果)는 다음과 같다: 1) 종간(種間) 교잡종중(交雜種中)에서 P. rigida와 P. taeda 간(間)의 교잡종(交雜種)들의 생장(生長)이 우수(優秀)하였으며, 특(特)히 P. taeda ${\times}$ P. rigida의 생장(生長)은 전북지역(全北地域)에서 그 우수성(優秀性)이 입증(立證)되었다. 2) P. taeda의 생존율(生存率)이 극(極)히 낮았는데, 이것은 내한력(耐寒力)이 약(弱)한 것에 기인(基因)한 것으로 보인다. 3) 재적생장(材積生長)은 구과(毬果)의 폭(幅) 및 길이, 엽(葉)과 엽초(葉鞘)의 길이와 고도(高度)의 상관관계(相關關係)가 있었다. 4) Cluster 분석(分析) 결과(結果) 교잡종(交雜種)들은 일반적(一般的)으로 양친수(兩親樹) 사이에 위치(位置)하였다.

• 한방재활의학과학회지
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• 제19권1호
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• pp.169-186
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• 2009

Obejectives : The electroacupuncture(EA) on regional fat is often used by oriental medicine doctors in Korea. However, there have been few studies about safety and biocompatibility of its application. Therefore we investigated the safety and change of mechanical character of electroacupuncture after its application on localized fat deposit. Methods : Online surveys were completed from Aug 21 2008 to Aug 28 2008, by email, the online survey data were obtained from nearly 10,002 Korean medical doctors who listed their email address on the website of association of korean oriental medicine. This study includes an experiment on the physical strength and biological property of long needles. We represent each method which were collected in advance survey, then sealed the needles off and did laboratory experiment, which includes surface observation, analysis, vickers hardness test, and also biocompatibility and toxicity test. Results : There was no considerable difference about the physical property after applying electric current, the cell survival rate did not change in comparison with controlled group either. Conclusions : Although it seems there are no considerable acupuncture-related problem, we still need additional studies about clinical effectiveness difference related operation condition. Besides, it will need research on the actual condition, standardization, and criteria about the comsumed needle in Korea.

• Journal of Veterinary Science
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• 제19권6호
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• pp.827-834
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• 2018

The purpose of this study was to evaluate the clinical and imaging characteristics of canine splenic tumors and to establish guidelines for the presurgical assessment of splenic tumors in dogs. Fifty-seven dogs that underwent total splenectomy for the treatment of splenic tumors were evaluated by examining medical records, hematologic results, diagnostic imaging results, and histopathologic results. The maximum lesion size from ultrasonography was significantly different between malignant and benign tumors (p = 0.002). There was a correlation between tumor margination and type of splenic tumors (p = 0.045). Precontrast lesion attenuation on computed tomography was significantly different between splenic malignant and benign tumors (p = 0.001). The mean ${\pm}$ SD precontrast lesion attenuation of malignant tumors was $40.3{\pm}5.9$ Hounsfield units (HU), and for benign tumors, it was $52.8{\pm}6.8HU$. In conclusion, some variables of the imaging examination could be used to distinguish the type of splenic tumor. Based on the study results, using a diagnostic flowchart would be effective in increasing the survival rate of patients with splenic malignant tumors. In addition, fine needle aspiration or magnetic resonance imaging prior to surgical exploration and histopathologic examination may be useful in achieving a more accurate diagnosis.

• Archives of Plastic Surgery
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• 제38권6호
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• pp.808-814
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• 2011

Purpose: Despite wide clinical use of breast implants, there is continued concern about the lifespan of these devices. The causes of explantation were infection, deflation of implant and patient's want. The deflation of saline-filled breast implant was related to strength and durability of implant shell. The purpose of this study is to evaluate the clinical durability of saline-filled breast implant through the analysis of duration until deflation occurred, causes, incidence and influencing factors. Methods: Retrospective analyses were conducted on clinical records for 19 cases of deflation of saline-filled breast implant from 201 cases of breast reconstruction with saline-filled implant between May 1995 and June 2011. The authors had been analyzed the causes of deflation, survival duration, symptom, sign, nipple excision, volume of implant, saline filling, method of reoperation, breast cancer stage and combined capsular contracture. Results: The causes of deflation were attributed to the cases that cannot be evaluated the causes in 15 cases, fall down in 1 case, mammography in 2 cases, accidental needle injury in 1 case. Mean survival duration was 4 years and 5 months. The duration of survival was less than 1 year for 5 cases, 1 year to 10 years for 10 cases, more than 10 years for 4 cases. The volume between 201 and 250 cc of deflated breast implant was rated as high by 14.0 percent. The deflation rate of underfilled implants was 11.4 percent, adequate filled implants was 9.3 percent. None of overfilled implant was deflated. The deflation of smooth surface implant was 5 of 152 cases. Textured implant was 14 of 49 cases. The capsular contracture of non-deflated breast implant was 28 of 182 cases and that of deflated breast implant was 6 of 19 cases. Conclusion: The patients who underwent saline-filled breast implant implantation should be informed that their implant could deflate. The analysis of clinical durability and causes of deflation in breast implant was important for the prediction and prevention of reopeation. The authors could suppose the causes of deflation of saline-filled breast implant through history, duration of survival, inspection of the shell of implant.