• Microbiology and Biotechnology Letters
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• v.31 no.4
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• pp.395-399
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• 2003

Nebramycin is a complex of aminocyclitol compounds that is produced by aerobic culture in fermentation process. The major antibiotic factors produced by Streptoalloteichus hindustanus are nebramycin factor 2, 4, 5'and kanamycin A. A mutant was selected, producing nebramycin factor 5' activity 16.4 times higher than parent strain by microbiological assay using Pseudomonas aeruginosa CH-U34AF. The component ratio of nebramycin factor 5' was dramatically increased from 34% to 70% by the optimization of fermentation condition. It was found that the component ratio of nebramycin factor 5' in fermentation was especially affected by the oxygen transfer rate. Optimum oxygen transfer rate for maximal nebramycin factor 5' productivity and ratio during S. hindustanus fermentation was elucidated to $0.50 mMO_2$/min.

• YAKHAK HOEJI
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• v.37 no.1
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• pp.1-8
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• 1993

A procedure for the high-performance liquid chromatographic determination of Nebramycin factors in fermentation broth of Streptoalloteichus hindustanus ATCC 31218 mutant was investigated using pre-column derivatization and LTV detection. The method is based on pre-column derivatization of Nebramycin factors with 2,4-dinitrofluorobenzene(DNFB) in the presence of Tris (hydroxymethyl)aminoethane. The chromatographic separation of derivatives of Nebramycin factors and unknown impurities is achieved using reversed-phase column (NOVA-PAK $C_{18}$, Waters Co.) and AcCN : H$_{2}$O : AcOH (53.0:46.5:0.5) as a mobile phase. The mixture of these derivatives were separated within 35 minutes and the optimum wavelength($\lambda_{max}$ ) of the UV detector was 353 nm. The linearity of response for derivatives of Nebramycin factors is demonstrated for concentrations up to 500 $\mu\textrm{g}$/ml and the relative standard deviation is less than 0.79%. Detection limit was 1.67 ng for the 10 $\mu\textrm{l}$ sample volume employed.