• Asian Pacific Journal of Cancer Prevention
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• v.16 no.13
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• pp.5525-5529
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• 2015

Background: Currently it is believed that human papillomaviruses (HPV) are associated with the development of some oral/oropharyngeal cancers. It has been suggested that these viruses influence carcinogenesis in both smokers and non-smokers. Data on the prevalence of HPV in healthy adults are thus needed to estimate the risk of oral/oropharyngeal cancer. The aim of this study was to assess the prevalence of oral HPV in healthy female adults in Indonesia and Thailand. Materials and Methods: Healthy female students from the Faculties of Dentistry of Universitas Indonesia and Chiang Mai University were asked to participate in this pilot study. DNA was extracted from saliva specimens and screened for HPV16 and HPV18 using PCR. Results: The age, marital status and sexual experience of the subjects between the two countries were not significantly different. Eight (4%) and 4 (2%) samples were positive for HPV16 and HPV18, respectively. Fisher's Exact test found a significant difference between HPV16 positivity in subjects who were married and had sexual intercourse but not for HPV18. Conclusions: This study successfully detected presence of HPV16 and HPV18 DNA in a number of saliva samples from female dental school students. Marital status, experience of sexual intercourse and safe sexual practice are related to the possibility of finding HPV DNA finding in saliva. Dentists, physicians and other health care professionals may gain significant value from the findings of this study, which provide an understanding of the nature of HPV infection and its risk to patient health and disease.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.10 no.1
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• pp.104-114
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• 2000

This study was carried out to estimate current status of dust concentrations in the factories producing non-metallic products in Korea. Data were gathered from the environmental monitoring reports by 40 workplace environmental monitoring institutes and the questionnaire designed for the study by the authors. A total of 1838 dust samples from 368 plants were obtained from the reports and were analyzed with no data modifications. But data on asbestos, rock wool, and welding fumes were excluded in this evaluation. The factories were classified into eight groups according to the standard industrial classification scheme in Korea. The results of this study were as follows; 1. The factories included were glass, non-refractory ceramic, refractory ceramic ware, structural non-refractory clay and ceramic, cement and lime, articles of concretes, and stone. Mean(geometric mean) concentrations of total dust samples ranged from $1.75(0.84)mg/m^3$ to $5.87(2.84)mg/m^3$. Statistically significantly higher dust concentrations were found in the cement and lime industries compared with other industries. 2. The non-compliance rates were 15.2% in glass and 20.6% in other non-metallic mineral products industries. Although all institutions surveyed utilized the identical sampling and classification scheme for dusts as specified in the current occupational exposure limits, wide discrepancies were found in collecting samples and classifying dusts. Further problems were discovered in classifying dusts. A dust sample collected could be classified into any of the three groups regardless of silica content. The results of this study showed that dust concentrations in the non-metallic products industries varied widely. Also discovered was classification errors of dust types among workplace monitoring institutes. These errors could adversely affect the results of exposure assessments and the true nature of dust hazards. Further, no institutions performed respirable dust sampling and analysis of crystalline silica. In order to correct these malpractices, current standards of occupational exposure limits should be revised and tight supervision by the Ministry of Labour be suggested.

• The Korea Journal of Herbology
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• v.21 no.2
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• pp.109-119
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• 2006

Objectives : This study was performed to approach of properties-flavours theory and the study of morphological standard in CORNI FRUCTUS. Methods : The properties-flavours theory were attempted with bibliographic method and various climate-information. The external-internal morphological standards were determined by using stereoscope and butanol series. Results and conclusion : 1. The significant analysis for the corni fructus as the properties-flavours theory, is a follow-up survey of the effect written in the official oriental medicine book. 2. The other analytic methods according to the gathering time, processing or storing system or especially nature or growth environment(altitude, temperature,. the mean moisture, and the agronomical survey) by way of the explanation of properties-flavours theory appears so many insufficiency at the many cases. 3. The external and internal characteristics according to the shape of original plant or herbal states, entirely correspond to all the literature on this subject. 4. According to the place of production, there are some different points. (l) In the external shapes, it is reasonable that if we make the basic standard of classification by the sharpness of color, the existence of gloss and the size of products. In the case of the Korean products, it shows bright and glossy color totally, but in the case of North Korea products, it shows black and lackluster. And in the case of the foreign products, it has softer body than Korea one and shows white color on the surface. (2) In the internal shapes, the Korea products has a thicker cuticle layer than the foreign one, and has fewer ca-oxalate in the parenchyma cell.

• The Korean Journal of Physiology and Pharmacology
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• v.8 no.4
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• pp.219-225
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• 2004

The pelvic ganglia provide autonomic innervations to the various urogenital organs, such as the urinary bladder, prostate, and penis. It is well established that both sympathetic and parasympathetic synaptic transmissions in autonomic ganglia are mediated mainly by acetylcholine (ACh). Until now, however, the properties of ACh-induced currents and its receptors in pelvic ganglia have not clearly been elucidated. In the present study, biophysical characteristics and molecular nature of nicotinic acetylcholine receptors (nAChRs) were studied in sympathetic and parasympathetic major pelvic ganglion (MPG) neurons. MPG neurons isolated from male rat were enzymatically dissociated, and ionic currents were recorded by using the whole cell variant patch clamp technique. Total RNA from MPG neuron was prepared, and RT-PCR analysis was performed with specific primers for subunits of nAChRs. ACh dose-dependently elicited fast inward currents in both sympathetic and parasympathetic MPG neurons $(EC_{50};\;41.4\;{\mu}M\;and\;64.0\;{\mu}M,\;respectively)$. ACh-induced currents showed a strong inward rectification with a reversal potential near 0 mV in current-voltage relationship. Pharmacologically, mecamylamine as a selective antagonist for ${\alpha}3{\beta}4$ nAChR potently inhibited the ACh-induced currents in sympathetic and parasympathetic neurons $(IC_{50};\;0.53\;{\mu}M\;and\;0.22\;{\mu}M,\;respectively)$. Conversely, ${\alpha}-bungarotoxin$, ${\alpha}-methyllycaconitine$, and $dihydro-{\beta}-erythroidine$, which are known as potent and sensitive blockers for ${\alpha}7$ or ${\alpha}4{\beta}2$ nAChRs, below micromolar concentrations showed negligible effect. RT-PCR analysis revealed that ${\alpha}3$ and ${\beta}4$ subunits were predominantly expressed in MPG neurons. We suggest that MPG neurons have nAChRs containing ${\alpha}3$ and ${\beta}4$ subunits, and that their activation induces fast inward currents, possibly mediating the excitatory synaptic transmission in pelvic autonomic ganglia.

• Journal of The Korean Society of Clinical Toxicology
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• v.9 no.1
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• pp.20-25
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• 2011

Purpose: This study was designed to analyze the contributing factors, as well as the incidence and nature of the cardiac toxicity, in patients presenting with diphenhydramine overdose. Methods: We retrospectively reviewed the medical records of the intoxicated patients who presented to the ED of Korea University Anam Hospital from January 2008 to December 2010. Those patients who visited due to a diphenhydramine overdose were selected and the following features were recorded for analysis: the general characteristics, vital signs, the amount of ingested diphenhydramine, the time interval from ingestion to presentation, the coingested drugs (if any), the toxicities and the ECG findings. Cardiac toxicity, while defined mainly in terms of the temporary ECG changes such as QTc prolongation, right axis deviation, QRS widening, high degree AV block and ischemic changes, also encompassed cardiogenic shock, which is a clinical finding. Results: A total of eighteen patients were enrolled. Of the eighteen patients, eight had ingested diphenhydramine only, while ten had ingested other drugs in addition to diphenhydramine. The most commonly observed toxicity following diphenhydramine overdose included cardiac toxicity (78%). Cardiac toxicity was observed in all the patients who presented to the emergency department 2 hours after ingestion. The patients with QTc prolongation turned out to have ingested significantly larger amounts of diphenhydramine. Conclusion: QTc prolongation and right axis deviation were common findings for the patients with a diphenhydramine overdose. QTc prolongation was more likely to occur with ingesting larger amounts of diphenhydramine. Close monitoring is mandatory for patients who have ingested large amounts of diphenhydramine to prevent such potentially lethal cardiac toxicity.

• Journal of Sasang Constitutional Medicine
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• v.13 no.2
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• pp.94-109
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• 2001

1. Background and Purpose ${\ulcorner}$DongyiSooseBowon${\lrcorner}$ is the book that was mixed ${\ulcorner}$GaboBon${\lrcorner}$ and ${\ulcorner}$KyungjaBon${\lrcorner}$ which was written by DongMu. But hardly any clause or text of the GaboBon excepting ${\ulcorner}$DongyiSooseBowon${\lrcorner}$ has been described. At the year of 2000, the society of sasang constitutional medicine acqired ${\ulcorner}$HamsanSachon DongyiSooseBowon GaboGubon${\lrcorner}$ which is seen as a written copy of ${\ulcorner}$GaboBon${\lrcorner}$. According to Lee Sung-Su who is the great-grandson of DongMu's older brother, ${\ulcorner}$HamsanSachon DongyiSooseBowon GaboGubon${\lrcorner}$ was worked by Lee Jin-Yoon who is the grandson of DongMu's older brother, and it was copied by Han Min-Gab and now is owned by Lee Sung-Su who is son of Lee Jin- Yoon. 2. Methods This paper was written in order to understand of ${\ulcorner}$HamsanSachon DongyiSooseBowon GaboGubon${\lrcorner}$ through the study to focus on the bibliographical character. 3. Result and Conclusion 1) It is reasonable that ${\ulcorner}$HamsanSachon DongyiSooseBowon GaboGubon${\lrcorner}$ had copied to the original ${\ulcorner}$DongyiSoose Bowon GaboGubon${\lrcorner}$ which was written by DongMu. 2) We can find the character of ${\ulcorner}$HamsanSachon DongyiSooseBowon GaboGubon${\lrcorner}$ as follows. (1) It has the table of contents, the definition of the number of the texts, and the article number of each texts. (2) ${\ulcorner}$KyungJaBon${\lrcorner}$ and ${\ulcorner}$SinchukBon${\lrcorner}$ has different number of the texts about ${\ulcorner}$The general remarks on the Soyangin${\lrcorner}$. (3) It is different with original ${\ulcorner}$GaboBon${\lrcorner}$ because of the extracting marks of the text. (4) It is supposed that it has the pares which was additionally or wrongly amended by someone. 3) We can consider that the character of original ${\ulcorner}$GaboBon${\lrcorner}$ which was written by DongMu from ${\ulcorner}$HamsanSachon DongyiSooseBowon GaboGubon${\lrcorner}$ as follows. (1) ${\ulcorner}$The Discourse on Nature and Order${\lrcorner}$${\sim}$${\ulcorner}$The Discourse on the Four Principles${\lrcorner}$ is original articles of ${\ulcorner}$GaboBon${\lrcorner}$. (2) The head title of${\ulcorner}$The Discourse on the Origin of Oriental Medicine${\lrcorner}$ was not in ${\ulcorner}$GaboBon${\lrcorner}$. (3) It is not clear whether ${\ulcorner}$The Discourse on the General Health Maintenance${\lrcorner}$ and ${\ulcorner}$The Discourse on Identifying Four Constitutions${\lrcorner}$ is original texts or amended texts of ${\ulcorner}$GaboBon${\lrcorner}$. So it is required more study about it.

• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1326-1334
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• 2008

The prime-boost vaccination with DNA vaccine and recombinant viral vector has emerged as an effective prophylactic strategy to control infectious diseases. Here, we compared the protective immunities induced by multiple alternating immunizations with DNA vaccine (pCIgB) and replication-incompetent adenovirus (Ad-gB) expressing glycoprotein gB of pseudorabies virus (PrV). The platform of pCIgB-prime and Ad-gB-boost induced the most effective immune responses and provided protection against virulent PrV infection. However, priming with pCIgB prior to vaccinating animals by the DNA vaccine-prime and Ad-boost protocol provided neither effective immune responses nor protection against PrV. Similarly, boosting with Ad-gB following immunization with DNA vaccine-prime and Ad-boost showed no significant responses. Moreover, whereas the administration of Ad-gB for primary immunization induced Th2-type-biased immunity, priming with pCIgB induced Th1-type-biased immunity, as judged by the production of PrV-specific IgG isotypes and cytokine IFN-$\gamma$. These results indicate that the order and injection frequency of vaccine vehicles used for heterologous prime-boost vaccination affect the magnitude and nature of the immunity. Therefore, our demonstration implies that the prime-boost protocol should be carefully considered and selected to induce the desired immune responses.

• The Journal of Korean Medicine
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• v.19 no.1
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• pp.430-443
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• 1998

The research on the actual condition of the oriental health related industry has been made with defining it's phase. As a result, I have come to develope korean-type health model, which can solve the problems derived from the past herb medicine industry. The prospects and roles of the oriental health related industries are as follows: The phase adjustment of the oriental health related industry. The problems of modern medical science happens in the beginning of 21st century, the importance of oriental health improvement in the field of health business is now being on the rise. therefore it is necessary to readjust the present status of the oriental medicine and to take it into health area. Should the responsible institutes regarded on oriental medicine make clear prescription in terms of oriental health industry's role and limitation, they could contribute health circumstance in the 21st century. This is because oriental health related industry can be utiliged in not only protective area and curable area. The present situation of the oriental health related industry. These herb medical societies are flooded with various oriental health industry apart from the legal systematic control so far health industry apart from the legal systematic control so far. The expected survey can not be complete but random under these situations. Nevertheless oriental medical health industry area such as a diet cure, exercise treatment and living condition cure are being considerably expanded, especially living facilities improvement and mind control areas are toward worldwide. Consquently herb health industries are now？ consistantly developed by the influence of western nature treatment stemmed from the oriental medicine theory. The role and prospect of the oriental health related industry in 21st century. The oriental health industries can be helpful for the human being's health improvement in general but by the lack of scholastic oriental health theory, it does not contribute to the health welfare. If these problems are made up for in the field of theory area, the oriental health related industry will surely have the potentiallity and know how for health welfare. The successive role of these industry is depended on the fact that the characteristics of the oriental medicine should adjust to the real society with the systematic management. In the 21C, oriental health industry will certainly be vitalized owing to the social and periodical request especially in the area of oriental medical industry, oriental health drink industry, oriental health mind exercise industry, oriental health leasure industry and oriental health complex management industry. Among these areas, it is the oriental health complex amagement industry that will be the limestone of prosperity of the oriental health industry in 21C. Proposal for the social contribution of the oriental health related industry in the 21C. To achive the ultimate role of the oriental health industry in the 21c, first we should know how to use the oriental medicine. Second We should make an effort to revitalize the oriental health industry. So it is indespensible that government, academic institution and enterprise should concentrate their interest on the oriental health industry with common knowledge and systematic management in advance.

• Radiation Oncology Journal
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• v.36 no.2
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• pp.147-152
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• 2018

Purpose: To evaluate the treatment outcomes of adjuvant external beam radiation therapy (EBRT) and vaginal brachytherapy (VB) following radical hysterectomy in cervical cancer patients with involved vaginal resection margin (VRM). Materials and Methods: We retrospectively reviewed the medical records of 21 patients treated with postoperative EBRT and VB for positive VRM FIGO stage IB-IIA cervical cancer between 2003 and 2015. Concurrent platinum-based chemotherapy was administered to all patients. Results: The median whole pelvis EBRT dose was 50.4 Gy (range, 45 to 50.4 Gy). In the VB, the median dose per fraction, number of fractions, and total dose delivered were: 4 Gy (range, 3.0 to 4.0 Gy), 4 fractions (range, 3 to 5 fractions), and 16 Gy (range, 12 to 20 Gy), respectively. At a median follow-up of 46 months (range, 9 to 122 months), local recurrence was observed in 2 patients, and distant metastasis was present in 7 patients. All patients with local recurrence subsequently developed distant metastases. The 5-year local control, disease-free survival, and overall survival rates were 89.1%, 65.9%, and 62.9%, respectively. Of the 21 patients, 7 patients (33.3%) reported grade 2 acute toxicity; however, there were no grade 3 or higher acute adverse events. Grade 1-2 late toxicities were observed in 8 patients. Late grade 3 urinary toxicity was reported in 1 patient. Conclusions: Adjuvant EBRT and VB showed excellent local control and low toxicity in cervical cancer patients with positive VRM. Although limited by its retrospective nature, the findings from our study provide evidence supporting the use of additional VB in pathologically involved VRM.

• Food Science and Preservation
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• v.24 no.8
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• pp.1122-1128
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• 2017

Pumpkin has long been used as traditional health materials in oriental medicine, pharmacy, medicine, and food industries in many countries. In this study, the water extract and two active components from tendril of young C. moschata Duch. were investigated on inflammation inhibitory activity. The water extract of young C. moschata Duch. showed high cell viability over 95% and it decreased the production of interlukin 6 (IL-6) and tumor necrosis factor (TNF-${\alpha}$) in the capacity of mouse bone marrow-derived macrophages (BMDM) upon lipopolysaccharide (LPS) stimulation. Also, isolated fraction (B4) suppressed the secretion of IL-6 and TNF-${\alpha}$. Among the domestically cultivated pumpkins, B4 fraction contained in the tendril part of them and it comprised of the order of tendril from Cucurbita pepo var. cylindrica, old of C. moschata Duch., and young of C. moschata Duch. These results suggest that water extracts of C. moschata Duch. and purified active compound, rutin, show anti-inflammation activity by suppression of the secretion of IL-6 and TNF-${\alpha}$. It can be applicable as pharmaceutical materials.