• Journal of Oral Medicine and Pain
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• 제33권3호
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• pp.229-240
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• 2008

동물성 mucin은 인체 타액 mucin과 유사한 구조적 특성을 가지고 있으므로 효과적인 타액대체제의 개발에 적합한 성분으로 여겨져 왔다. 구강건조증 환자가 동물성 mucin 함유 타액대체제를 사용할 경우, 동물성 mucin과 인체 타액에 존재하는 항균 단백질이 용액상태인 전타액과 구강표면에 형성된 pellicle에 동시에 존재할 수 있으므로 이들 물질사이에 상호작용이 일어 날 수 있을 것이다. 본 연구의 목적은 용액과 hydroxyapatite(HA) 표면에서 동물성 mucin이 peroxidase 활성에 미치는 영향을 평가하기 위하여 시행되었다. 동물성 mucin이 peroxidase 활성에 미치는 영향은 돼지의 위장 mucin(porcine gastric mucin, PGM) 이나 소의 악하선 mucin(bovine submaxillary mucin, BSM)을 소의 lactoperoxidase(bovine lactoperoxidase, bLPO)나 타액검체와 incubation하는 방법을 사용하여 분석하였고, 표면상태에서의 연구를 위해 HA beads, HA disc, 소의 치아와 같은 3가지 종류의 HA 표면을 활용하였다. Peroxidase 활성은 NbsSCN 법을 이용하여 분석하였다. 1. 돼지위장 mucin은 용액상태에서 bLPO 활성을 증가시켰으나 타액검체의 peroxidase(peroxidase in saliva sample, POS) 활성에는 영향을 미치지 않았다. 2. 소 악하선 mucin은 용액상태에서 bLPO와 POS 활성에 영향을 미치지 않았다. 3. HA 표면에 부착된 돼지위장 mucin은 peroxidase의 부착과 활성을 증가시켰고 이러한 효과는 세 종류의 HA 표면 모두에서 일어났으며, POS의 활성증가는 HA beads와 소 치아 표면에서만 나타났다. 4. bLPO와 돼지위장 mucin의 혼합물을 HA 표면에 부착시킬 경우, HA beads와 HA disc 표면에서의 peroxidase 활성은 증가하였다. 5. bLPO의 돼지위장 mucin에 대한 부착친화도는 소 악하선 mucin에 비해 컸다. 이상의 결과를 종합해 볼 때 동물성 mucin은 용액상태와 HA 표면에서 peroxidase 활성에 영향을 미침을 알 수 있으며, 동물성 mucin을 포함하고 있는 타액대체제는 인체타액 및 타액대체제에 있는 peroxidase 활성에 영향을 미칠 수 있을 것이다.

• 대한치과보철학회지
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• 제46권1호
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• pp.1-11
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• 2008

현대 치의학에서는 환자의 심미적 만족을 충족시키기 위한 지속적인 관심으로 완전 도재관의 발전을 가져왔다. 최근의 연구 결과들은 완전 도재관의 물리적 성질과 마모 저항성, 색조의 안정성 등을 보고하고 있다. 이와 같이 여러 장점이 많음에도 불구하고 아직 파절에 대한 저항성에는 많은 의문점을 내포하고 있다. 구강 내에서 기능적인 교합력은 수복물의 변연에 응력을 주게된다. 이러한 응력은 주변 조직으로 잘 분산되어져야 하는데, 수복물의 변연 형태에 따라 다른 양상을 보여주게 된다. 변연 형태는 수복물의 부피와 모양, 그리고 변연에서의 적합도에도 영향을 주게 된다. 본 연구에서는 완전 도재관에서의 변연 형태에 따른 응력 분포를 3차원 유한 요소 분석법을 이용하여 조사하였다. 상악 중절치가 기본 모델로 사용되었고, 완전 도재관을 재현하였다. 변연 형태로는 $90^{\circ}$ shoulder, $110^{\circ}$ shoulder, $135^{\circ}$ shoulder형태를 부여하고, 100N의 힘을 치축에 $45^{\circ}$ 방향으로 가하였다. 그 결과 도재 내부의 응력 분포는 $90^{\circ}$, $110^{\circ}$ 모델에 비해 변연 각도가 $135^{\circ}$인 모델의 경우가 응력의 분포가 고르게 나타났다. 그러나 변연에서는 $90^{\circ}$와 $110^{\circ}$ 모델의 응력보다 조금 더 집중되었다. $90^{\circ}$와 $110^{\circ}$ 모델의 비교시는 대체로 비슷했다. 알루미나 코아 내부의 응력 분포에서도 또한 $135^{\circ}$인 모델이 $90^{\circ}$와 $110^{\circ}$ 모델의 응력분포보다 더 잘 분포되었다. $90^{\circ}$와 $110^{\circ}$ 모델의 비교시는 $110^{\circ}$가 $90^{\circ}$ 모델에 비해 더 좋은 응력분포를 보였다. 알루미나 코아와 접착제 사이의 계면에서는 응력이 변연으로 갈수록 감소하고, 알루미나 코아와 도재 사이의 계면에서는 변연으로 갈수록 응력이 점차 증가하였다. $135^{\circ}$ shoulder는 고른 응력분포임에도 변연에서의 응력 집중이 나타나므로 완전 도재관의 변연으로는 적절하지 못하며, $90^{\circ}$ shoulder보다 상대적으로 좋은 응력분포와 변연 형성의 용이성이 우세한 $110^{\circ}$ shoulder가 완전 도재관의 변연에 추천될 수 있겠다.

• 대한치과보철학회지
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• 제31권4호
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• pp.580-610
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• 1993

The purpose of this study was to analyse the deflection and stress distribution at the supporting bone and it's superstructure by the alteration of angulation between implant and it's implant abutment. For this study, the free-end saddle case of mandibular first and second molar missing would be planned to restore with fixed prosthesis. So the mandibular second premolar was prepared for abutment, and the cylinder type osseointegrated implant was placed at the site of mandibular second molar for abutment. The finite element stress analysis was applied for this study. 13 two-dimensional FEM models were created, a standard model at $0^{\circ}$ and 12 models created by changing the angulation between implant and implant abutment as increasing the angulation mesially and distally with $5^{\circ}$ unittill $30^{\circ}$. The preprocessing decording, solving and postprocessing procedures were done by using FEM analysis software PATRAN and SUN-SPARC2GX. The deflections and von Mises stresses were calculated under concentrated load (load 1) and distributed load(load 2) at the reference points. The results were as follows : 1. Observing at standard model, the amount of total deflection at the distobuccal cusp-tip of pontic under concentrated load was largest of all, and that at the apex of implant was least of all, and the amount of total deflection at the buccal cusp-tip of second premolar under distributed load was largest of all, and that at the apex of implant was least of all. 2. Increasing the angulation mesially or distally, the amounts of total deflection were increased or decreased according to the reference points. But the order according to the amount of total deflection was not changed except apex of second premolar and central fossa of implant abutment under concentrated load during distal inclination. 3. Observing at standard model, the von Mises stress at the distal joint of pontic under concentrated load was largest of all, and that at the apex of implant was least of all. The von Mises stress at the distal margin of second premolar under distributed load was largest of all, and that at the apex of Implant was least of ail. 4. Increasing the angulation of implant mesially, the von Mises stresses at the mesial crest of implant were increased under concentrated load and distributed load, but those were increased remarkably under distributed load and so that at $30^{\circ}$ mesial inclination was largest of all. 5. Increasing the angulation of implant distally, the von Mises stresses at the distal crest of implant were increased remarkably under concentrated load and distributed load, and so those at $30^{\circ}$ distal inclination were largest of all.

• 대한치과보철학회지
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• 제38권5호
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• pp.704-723
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• 2000

This study was undertaken to evaluate the tensile bond strength of In-Ceram alumina core treat-ed by ion assisted reaction(IAR). Ion assisted reaction is a prospective surface modification technique without damage by a keV low energy ion beam irradiation in reactive gas environments or reactive ion itself. 120 In-Ceram specimens were fabricated according to manufacturer's directions and divided into six groups by surface treatment methods of In-Ceram alumina core. SD group(control group): sandblasting SL group: sandblasting + silane treatment SC group: sandblasting + Siloc treatment IAR I group: sandblasting + Ion assisted reaction with argon ion and oxygen gas IAR II group: sandblasting + Ion assisted reaction with oxygen ion and oxygen gas IAR III group: sandblasting + Ion assisted reaction with oxygen ion only For measuring of tensile bond strength, pairs of specimens within a group were bonded with Panavia 21 resin cement using special device secured that the film thickness was $80{\mu}m$. The results of tensile strength were statistically analyzed with the SPSS release version 8.0 programs. Physical change like surface roughness of In-Ceram alumina core treated by ion assistad reaction was evaluated by Contact Angle Measurement, Scanning Electron Microscopy, Atomic Force Microscopy; chemical surface change was evaluated by X-ray Photoelectron Spectroscopy. The results as follows: 1. In tensile bond strength, there were no statistically significant differences with SC group, IAR groups and SL group except control group(P<0.05). 2. Contact angle measurement showed that wettability of In-Ceram alumina core was enhanced after IAR treatment. 3. SEM and AFM showed that surface roughness of In-Ceram alumina core was not changed after IAR treatment. 4. XPS showed that IAR treatment of In-Ceram alumina core was enabled to create a new functional layer. A keV IAR treatment of In-Ceram alumina core could enhanced tensile bond strength with resin cement. In the future, this ion assisted reaction may be used effectively in various dental materials as well as in In-Ceram to promote the bond strength to natural tooth structure.

• 한국어류학회지
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• 제1권1_2호
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• pp.83-92
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• 1989

한국산(韓國産)납자루아과어류(科魚類) 10종(種)의 인두치(咽頭齒)에 있어서의 교합구(咬合溝)와 제1치(第1齒) 저작면(咀嚼面) 및 기저후두골(基底後頭骨)의 저작돌기(咀嚼突起)의 형태(形態)를 비교(比較)하였다. 종전(從前)에 인두치(咽頭齒)와 교합면(咬合面)에 홈(溝)이 없다고 알려져 있는 종류(種類)(납자루, 칼납자루, 묵납자루, 납줄갱이, 줄납자루 등(等))에서도 축소(縮小)된 교합구(咬合溝)가 존재(存在)하고 있음이 밝혀졌다. 기저후두골(基底後頭骨)에 있는 저작돌기(咀嚼突起)의 형태(形態)는 그 전연부(前緣部)가 미발달(未發達)된 마제형(馬蹄形)을 이루는 경우와 전연부(前緣部)가 약간(若干) 들어간 계란형(鷄卵形)을 이루는 경우로 대별(大別)되었다. 또한 인두치(咽頭齒)의 교합구(咬合溝)는 축소(縮小)된 경우와 비교적(比較的) 발달(發達)되는 경우로 구분(區分)되었으며 제제1치(第1齒)의 저작면(咀嚼面)은 비교적(比較的) 미발달(未發達)된 경우와 비교적(比較的) 발달(發達)된 경우 및 잘 발달(發達)된 경우의 세가지로 구분(區分)되었다. 그리고 이들 형태변화(形態變化)는 납자루류(類)의 식성(食性)과 관련(關連)이 있는 것으로 추정(推定)되었다. 그리고 각시붕어의 교합면(咬合面)에도 발달(發達)된 홈이 있다고 알려져 있으나 이 교합구(咬合溝)는 축소(縮小)된 교합구(咬合溝)를 지니는 종(種)과 유사(類似)했다. 그러나 각시붕어의 기저후두골(基底後頭骨)의 저작돌기(咀嚼突起)의 형태(形態)는 마제형(馬蹄形)으로 납줄개속(屬) Rhodeus에서는 특이(特異)하였다. 이로 미루어 보면 각시붕어는 납자루아과어류중(亞科魚類中)에서는 원시적(原始的)인 종(種)과 이러한 형질(形質)을 공유(共有)하는 점(點)에서 납줄개속(屬) 중(中)에서는 비교적(比較的) 조상적(祖上的)인 종(種)이라고 생각되었다. 이러한 사실(事實)을 고려(考慮)하여 인두치계(咽頭齒系)를 이상(以上)의 세가지 형태적특징(形態的特徵)을 조합(組合)하여 한국산(韓國産)납자루아과어류(亞科魚類)를 구분(區分)한다면 두가지의 group 및 두가지의 type나 또는 다섯가지의 subtype으로 유형(類型)이 구분(區分)되었으며 이 결과(結果)는 일본산(日本産)납자루아과어류(亞科魚類)의 인두치계(咽頭齒系)에 바탕을 둔 Suzuki and Hibiya(1985)의 유형구분(類型區分)과 잘 일치(一致)했다.

• Journal of Periodontal and Implant Science
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• 제41권2호
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• pp.73-78
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• 2011

Purpose: For periodontal tissue engineering, it is a primary requisite and a challenge to select the optimum types of cells, properties of scaffold, and growth factor combination to reconstruct a specific tissue in its natural form and with the appropriate function. Owing to fundamental disadvantages associated with using a two-dimensional substrate, several methods of seeding cells into three-dimensional scaffolds have been reported and the authors have asserted its usefulness and effectiveness. In this study, we explore the cell attachment of periodontal ligament fibroblasts on nanohydroxyapatite (n-HA) scaffold using avidin biotin binding system (ABBS). Methods: Human periodontal ligament fibroblasts were isolated from the health tooth extracted for the purpose of orthodontic procedure. HA nanoparticles were prepared and $Ca(NO_3)_2-_4H_2O$ and $(OC_2H_5)_3P$ were selected as precursors of HA sol. The final scaffold was 8 mm in diameter and 3 mm in height disk with porosity value of 81.55%. $1{\times}10^5$ periodontal ligament fibroblasts were applied to each scaffold. The cells were seeded into scaffolds by static, agitating and ABBS seeding method. Results: The number of periodontal ligament fibroblasts attached was greater for ABBS seeding method than for static or agitating method (P<0.05). No meaningful difference has been observed among seeding methods with scanning electron microscopy images. However, increased strength of cell attachment of ABBS could be deduced from the high affinity between avidin and biotin ($Kd=10^{-15}\;M$). Conclusions: The high-affinity ABBS enhances the ability of periodontal ligament fibroblasts to attach to three-dimensionally constructed n-HA scaffold.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권5호
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• pp.428-433
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• 2004

Sinus floor augmentation has been proven an effective treatment procedure to increase bone volume in the posterior edentulous maxilla. Autogenous bone considered to be the best material for reconstructive bone surgery and has been successfully used as a graft material to augment posterior maxilla. However, the collection of autogenous bone required extra risks for morbidity and complaints. So, various bone graft materials included ${\beta}$-tricalcium phosphate(${\beta}$-TCP) has been introduced for replacing the autogenous bone. The objective of this clinical study was to determine the predictability of endosseous implant placed in a maxillary sinus with ${\beta}$-TCP grafting. We performed sinus elevation with ${\beta}$-TCP to install the implant in the 10 maxillary cases. The prosthetic procedure was performed 6-9 months after. The implant-prosthetics was checked about 1 year. We checked the implant and measured the maximum bite force to evaluate the function of the implant. There was not observed the specific problem and complication in dental implant and maxillary sinus in the grafted materials. The maximum bite force was 558N in case of natural tooth, 365N in implant without grafting, 318N in implant with ${\beta}$-TCP grafting. There was no significant difference between with and without sinus grafting on maximum bite force(p>0.05). As though the long term check-up is needed, the grafting of ${\beta}$-TCP as a osteoconductive materials can expand the volume and induce dense new bone formation in maxillary sinus. So, this short-term results support that ${\beta}$-TCP can be a suitable material for sinus augmentation.

• 대한심미치과학회지
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• 제22권1호
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• pp.47-55
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• 2013

치아의 상실로 인한 국소적 치조제 결손(localized alveolar defect)은 고정성 국소의치(fixed partial denture) 제작시에 많은 기능적, 심미적 문제점들을 야기한다. 특히 전치부에서의 치조제 결손 부위는 그 형태와 양상에 따라서 심미적 결과에 많은 영향을 미치게 되는데, 이 결손 부위의 회복 여부가 최종적인 심미적 결과의 성패를 좌우하게 된다. 치조제 결손의 양상은 발치 전 치조골의 상황과 염증 정도에 따라 다양하게 나타날 수 있는데, 그 양상은 크게 보면 수평적, 수직적, 혹은 복합적 결손 등으로 분류할 수 있다. 이러한 결손부의 형태와 양에 따라서 우리가 회복시켜 주어야 할 정도도 다르고, 그 난이도 또한 다르다고 할 수 있다. 여기에 추가적으로 고려되어야 할 요소가 결손부와 자연치가 만나는 proximal gingival height이다. 인접 치아와 접하는 gingival height는 그 부위의 치조골 높이에 많은 영향을 받게 되는데, 만약 이 부위에 결손이 존재한다면 역시 심미적 결과에 부정적인 영향을 줄 것이다. 하지만 결손부위 내에서도 치아와 인접한 부위의 gingival height를 동시에 증대시키기 위해 소개된 technique은 많지 않으며 시술의 예지성도 높지 않은 것이 사실이다. 이 case report에서 필자는 수평적 혹은 수직적 결손 및 복합적 결손에 다양하게 적용 가능하면서도 인접치아와 인접한 부위의 gingival height에도 도움이 될 수 있는 술식을 소개하려고 한다.

• Journal of Periodontal and Implant Science
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• 제34권1호
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• pp.71-81
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• 2004

Peri-implantitis could be the result of biomechanical and occlusal overload as well as microbiologic invasion. The dental implant may be more susceptible to dental plaque than the natural tooth, as the predictability of a stable soft tissue attachment complex has not yet been confirmed. With the development of peri-implantitis, the implant surface would be exposed to the oral environment and becomes coated with bacteria. The objective of therapy for this condition is to regain integration of the implant with bone. Since fibroblast adherence to surfaces is impeded by endotoxin, it would seem that decontamination would be desirable to obtain maximum osseointegration. The purpose of this study was to determine whether various chemotherapeutic and mechanical treatments(distilled water, air-powder abrasive, hypersaturated citric acid, tetracycline) can detoxify contaminated titanium implant surface by means of kinetic LAL test. Experimental rough surface titanium disks were fabricated. All of them were divided into two groups(A.a group and P.g group) and each contaminated by A. actinomycetemcomitans and P. gingivalis suspension. Contaminated disks were treated with distilled water, air-powder abrasive, citric acid and tetracycline, and then all disks were placed into LPS-free water for elution. The results were as follows : 1. In A.a group, LPS elute level of all test groups were significantly lower than control group(p<0.05). 2. In A.a group, LPS elute level of test 2, test 3 and test4 groups were significantly lower than that of control group(p<0.05). But, among the test 2, test 3, test4 groups, the significant differences were not detected. 3. In P.g group, LPS elute level of test 2, test 3 and test 4 groups were lower than that of control group(p<0.05). But, among the test groups, the significant differences were not detected. From the result of this study, it would be concluded that air-powder abrasive, hypersaturated citric acid and tetracycline treatments may be effective at reducing endotoxin level on rough titanium implant surfaces, and can be clinically used. But the treatments in peri-implantitis differentially impact osseointegration making one method clinically superior. To gain this knowledges, further molecular biologic and histopathologic studies should be developed.

• Journal of Oral Medicine and Pain
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• 제24권2호
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• pp.207-217
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• 1999

This study was performed to investigate the effects of clutch adaptation on the mandibular rotational torque movement in normal people. 69 dental students were selected for the study. Their mean age were 23.6 years and they did not present any signs and symptoms of temporomandibular disorders. $BioEGN^{(R)}$ with $Rotate^{(R)}$ program was used to observe and record the amount of mandibular rotational torque on protrusion, on right excursion, on left excursion, and on comfortable wide opening movement. The natural tooth contact movement and the movement with clutch were performed in the above four each mandibular movement. Clutch was made by the method used in $Pantronic^{(R)}$ clutch fabrication. Distance of slant frontal which was translatory trajectory in frontal plane and degree of rotational torque in horizontal and in frontal plane were recorded. The data obtained were processed with SPSSWIN program and the results were as follows : 1. Distance of slant frontal in each mandibular movement generally increased with clutch. 2. Degree of rotational torque in horizontal and in frontal plane on protrusion and on lateral excursions did not increase with clutch, but the degree on wide opening increased with clutch. 3. Degree of rotational torque in horizontal plane on protrusion and on lateral excursions did not show any difference between right and left side, but the degree in frontal plane on protrusion and on lateral excursions showed significant difference between right and left side. 4. Total amount of rotational torque from right and left sides on protrusion and lateral excursions were not increased with clutch, but the degree on wide opening movement was increased with clutch. And in this case, degree in horizontal plane was larger than that in frontal plane. 5. Correlation between total amount of rotational torque in horizontal plane and that in frontal plane were highly significant on protrusion and on lateral excursions with or without clutch, but the significant correlation on wide opening without clutch became not significant with clutch.