• 한국미생물·생명공학회지
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• 제49권1호
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• pp.45-56
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• 2021

Improved amylases were developed from protoplast fusants of two amylase-producing Aspergillus species. Twenty regenerated fusants were screened for amylase production using Remazol Brilliant Blue agar. Crude enzyme extracts produced by solid state fermentation of rice bran were assayed for activity. Three variable factors (temperature, pH and enzyme type) were optimized to increase the amylase activity of the parents and selected fusants using rice bran medium and solid state fermentation. Analysis of this optimization was completed using the Central Composite Design (CCD) of the Response Surface Methodology (RSM). Amylase activity assays conducted at room temperature and 80℃ demonstrated that Aspergillus designates, T5 (920.21 U/ml, 966.67 U/ml), T13 (430 U/ml, 1011.11 U/ml) and T14 (500.63 U/ml, 1012.00 U/ml) all exhibited improved function making them the preferred fusants. Amylases produced from these fusants were observed to be active over the entire pH range evaluated in this study. Fusants T5 and T14 demonstrated optimal activity under acidic and alkaline conditions, respectively. Fusants T13 and T14 produced the most amylase at 72 h while parents TA, TC and fusant T5 produced the most amylase after 96 h of incubation. Response surface methodology examinations revealed that the enzyme from fusant T5 was the optimal enzyme demonstrating the highest activity (1055.17 U/ml) at pH 4 and a temperature of 40℃. This enzyme lost activity with further increases in temperature. Starch hydrolysis using fusant T5 gave the highest yield of glucose (1.6158 g/100 ml). The significant activities of the selected fusants at 28 ± 2℃ and 80℃ and the higher sugar yields from cassava starch hydrolysis over their parental strains indicate that it is possible to improve amylase activity using the protoplast fusion technique.

• Weed & Turfgrass Science
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• 제6권3호
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• pp.235-248
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• 2017

본 연구는 $5-25^{\circ}C$ 조건의 자연실온에서 훼스큐 초종 간 발아력, 발아세 및 발아 피크 기간을 조사하고자 수행되었다. 자연 실온 환경에서 종자 발아력은 62.0-97.0% 사이로 나타났는데 가장 우수한 초종은 TF 'Olympic Gold' 품종으로 나타났다. 초종 간 종자 발아력은 광엽형인 TF 초종이 세엽형 보다 더 우수하였으며, 세엽형 훼스큐 초종 간 발아력은 SF>CF, HF>CRF 순서로 나타났다. 발아세의 경우 발아율이 50%에 도달할 때까지 소요되는 50% 발아세 속도부터 80% 발아세 속도까지 전체적인 초종 간 발아세 경향은 TF 초종이 가장 빨랐고 TF 다음으로는 CF 초종 이었다. 그리고 HF 및 SF 초종은 발아세 속도가 중간 정도로 나타났고, 훼스큐 속에서 발아세 속도가 가장 느린 초종은 CRF로 판단되었다. 하지만 발아환경에 따른 50% 및 70% 발아세 경향은 자연실온($5-25^{\circ}C$)보다는 ISTA 변온($15-25^{\circ}C$)에서 평균 약 4일 정도 더 빨랐으며, 실무적으로 훼스큐 종자 파종은 낮과 밤의 온도 차이가 ISTA 변온 환경에 근접할수록 바람직한 것으로 판단되었다. 훼스큐 속 초종 간 발아 피크 기간(Peak time II)은 광엽형 TF 품종이 0.73-1.84일, 평균 1.45일로 세엽형 품종 보다 더 짧게 나타났다. 그리고 세엽형 훼스큐 초종 간 발아 피크 기간은 2.16-11.15일 사이로 다양하였는데 SFCF>SF>HF>CRF 순서로 판단되었다. 전체 공시 품종에서는 TF 'Olympic Gold' 품종이 가장 우수하였고, 반대로 CRF 'Audubon' 품종이 가장 불량한 것으로 판단되었다. 즉 훼스큐 속 초종 및 품종에 따라 이러한 조성 속도 및 특성 차이가 크게 나타날 수 있기 때문에 잔디밭 조성 시 적절한 초종 및 품종 선택은 대단히 중요하다.

• 한국광물학회지
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• 제19권3호
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• pp.189-195
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• 2006

황철석에 대한 압축성 연구를 다이아몬드 앤빌 기기 및 방사광을 이용하고 에너지분산 X-선회절법을 적용하여 상온에서 시행하였다. 황철석의 체적탄성률은 실험조건과 실험기기에 따라 매우 큰 변이를 보이고 있는데 이는 압력전달매체의 특성에 따른 것으로 알려져 있다. $FeS_{2}#1$(황철석+NaCl)과 $FeS_{2}#2$(황철석+MgO)에 대해 압력전달 매체로 각각 NaCl과 유체를 이용하여 체적탄성률 138.9 GPa와 198.2 GPa를 각각 얻었다. 이러한 값은 시료방의 압력상태에 따른 예측 결과치에 대치되는 것으로 그 원인은 압력전달액체의 상변이에 따른 것으로 추정되며 이외에 고상검출기의 차이, E*d값의 차이 등도 영향을 미친 것으로 판단된다. 모든 실험은 포항가속기연구소 1B2 빔라인에서 시행되었다.

• Bulletin of the Korean Chemical Society
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• 제32권6호
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• pp.1859-1864
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• 2011

Two lanthanide complexes, $[Ln(NO_3)_2(H_2O)_3(L)_2](NO_3)(H_2O)$ {Ln = Eu (1), Tb (2); L = 2-(4-pyridylium)-ethanesulfonate, $(4-pyH)^+-CH_2CH_2-SO_3^-)$}, were prepared from lanthanide nitrate and 4-pyridineethanesulfonic acid in $H_2O$ under microwave-heating conditions. Complexes 1 and 2 are isostructural, and the lanthanide metal in both complexes is coordinated to nine oxygen atoms. The pyridyl nitrogen in the ligand is protonated to give a zwitter ion that possesses an $NH^+$ (pyridyl) positive end and an $SO_3^-$ negative end. All O-H and N-H hydrogen atoms participate in hydrogen bonds to generate a two-dimensional (complex 1) or a three-dimensional network (complex 2). Complex 1 exhibits an intense red emission, whereas complex 2 exhibits an intense green emission in the solid state at room temperature.

• 한국작물학회지
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• 제44권2호
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• pp.138-142
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• 1999

An experiment was carried out to find out the changes in morphology, sugars, and $\alpha$-amylase activity during the priming of rice seeds (Oryza sativa L. cv. ＇Ilpumbyeo＇). For priming, seeds were soaked in -0.6 MPa PEG solution at 15$^{\circ}C$ for 4 days (properly primed) and at $25^{\circ}C$ for 4 and 10 days (over-primed) and dried at room temperature. The size of coleoptile and differentiated leaves of properly primed seeds were bigger and coleoptile was separated from the other part of embryo compared with non-primed and over-primed seeds. As priming of seeds advanced, compound starch grains in the endosperm disintegrated into tiny starch granules, and small holes were found in the tiny starch granules and a cavities developed between embryo and endosperm. The radicle and plumule of properly primed germinating seeds developed faster than non-primed and overprimed germinating seeds. Sucrose, maltose, and raffinose contents of properly primed seeds decreased, while content of glucose and fructose and $\alpha$-amylase activity increased. However, sugar content and $\alpha$-amylase activity of over-primed seeds were lower compared with non-primed seeds or properly primed seeds.

• 한국화재소방학회논문지
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• 제19권4호
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• pp.18-25
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• 2005

자연대류유동 형태의 실내화재 해석은 Zone 모델과 Field 모델로 대별된다. Zone 모델은 화염과 연소가스로 인한 상부의 고온 연기층과 하부의 찬 공기층의 온도를 균일하게 간주하고, 실험값을 대입한 간단한 대수식으로 계산하여 간편하나 각각의 위치에 대한 온도와 유속 등을 구할 수 없다. Field모델은 해석영역을 다수의 격자로 구성하고 운동량, 에너지방정식 등을 수치해석하는 방법으로 많은 계산시간 및 컴퓨터 용량이 필요하나 각각의 위치에서의 유동 및 온도분포 등을 자세히 규명 할 수 있다. 열유동 수치해석에는 기존의 상용 프로그램들이 있어 강제대류에는 잘 적용되나, 자연대류에는 대부분 만족할 만한 결과를 주지 못하고 있다. 이에 본 연구에서는 실내화재해석의 기초연구로, 바닥면이 부분적으로 가열될 때 우측벽의 경계조건을 변화시켜 가면서 실내의 층류 자연대류로 인한 유동장과 온도장을 SIMPLE 수치해석방법을 이용하여 직접 수치해석 하였다.

• 한국해양공학회지
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• 제33권6호
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• pp.547-555
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• 2019

The purpose of this study was to establish a numerical model of polyurethane foam (PUF) to simulate the dynamic response and strength of membrane-type Liquefied natural gas (LNG) Cargo containment system (CCS) under the impact load. To do this, initially, the visco-plastic behavior of PUF was characterized by testing the response of the PUF to the impact loads with various strain rates as well as PUF densities at room temperature and at cryogenic conditions. A PUF material model was established using the test results of the material and the FE analysis. To verify the validation of the established material model, simulations were performed for experimental applications, e.g., the dry drop test, and the results of FEA were compared to the experimental results. Based on this comparison, it was found that the dynamic response of PUF in dry drop tests, such as the reaction force and fracture behaviors, could be simulated successfully by the material model proposed in this study.

• 한국물환경학회지
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• 제30권2호
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• pp.199-205
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• 2014

The knowledge on the migration of antimony (Sb) from PET bottles into the water is of greate concern. Antimony in all bottled water marketed in korea and in raw water was analyzed. The detection rate of antimony in total bottled water was 88 % and 100% in PET (Polyethylene terephthalate, PET), 55% in PC (Polycarbonate, PC) bottled water. 55% of raw water contained antimony. The average concentration of Sb in PET bottled water was $0.39{\mu}g/L$, higher than PC bottles ($0.20{\mu}g/L$) and the raw water ($0.22{\mu}g/L$). The migration of Sb into water that is stored in different conditions (room temperature, $45^{\circ}C$, and direct sunlight exposure) was investigated for 180 days. The migration tendency increased with the storage time and temperature. PET bottles showed a sharp increase of Sb concentration at $45^{\circ}C$, but there was no differences between the room temperature and sunlight exposure. The Sb migration in all simulated solution(deionized water, 4% acetic acid, and 20% ethanol) also increased with storage time and temperature. The Sb migration values ranged from 0.35 to $0.49{\mu}g/L$ in all simulated solution, which was far below the permissible korean migration level of $40{\mu}g/L$. There was a tendency that the number of re-use of a bottle and the amount of leaching were in inverse proportion.

• Elastomers and Composites
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• 제51권1호
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• pp.1-9
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• 2016

Characterization of the UV oxidation for raw natural rubber (NR) was investigated in controlled conditions through image and FT-IR analysis. The UV oxidation was performed on a thin film of natural rubber coated on a KBr window at 254 nm and room temperature to exclude the thermal oxidation. Before or after exposure to UV light, image of the NR thin film was observed at a right or tilted angle. FT-IR absorption spectra were measured in transmission mode with the UV irradiation time. The UV oxidation of NR was examined by the changes of absorption peaks at 3425, 1717, 1084, 1477, 1377, and $833cm^{-1}$ which were assigned to hydroxyl group (-OH), carbonyl group (-C=O), carbon-oxygen bond (-C-O), methylene group $(-CH_2-)$, methyl group $(-CH_3)$, and cis-methine group $(cis-CCH_3=CH-)$, respectively. During the initial exposure period, the results indicated that the appearance of carbonyl group was directly related to the reduction of cis-methine group containing carbon-carbon double bond (-C=C-). Most of aldehydes or ketones from carbon-carbon double bonds were formed very fast by chain scission. A lot of long wide cracks with one orientation at regular intervals which resulted in consecutive chain scission were observed by image analysis. During all exposure periods, on the other hand, it was considered that the continuous increment of hydroxyl and carbonyl group was closely related to the decrement of methylene and methyl group in the allylic position. Therefore, two possible mechanisms for the UV oxidation of NR were suggested.

• 공업화학
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• 제34권2호
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• pp.199-205
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• 2023

Arsenic is a highly toxic element, and its contamination is widespread around the world. The natural materials with high selectivity and efficiency toward pollutants are important in wastewater treatment technology. In this study, the mesoporous synthetic hectorite was synthesized by facile hydrothermal crystallization of gels comprising silica, magnesium hydroxide, and lithium fluoride. Additionally, the naturally available clay was modified using zirconium at room temperature. Both synthetic and modified natural clays were employed in the removal of arsenate from aquatic environments. The materials were fully characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transform-infrared (FT-IR) analyses. The synthesized materials were used to remove arsenic (V) under varied physicochemical conditions. Both materials, i.e., Zr-bentonite and Zr-hectorite, showed high percentage removal of arsenic (V) at lower pH, and the efficiency decreased in an alkaline medium. The equilibrium-state sorption data agrees well with the Langmuir and Freundlich adsorption isotherms, and the maximum sorption capacity is found to be 4.608 and 2.207 mg/g for Zr-bentonite and Zr-hectorite, respectively. The kinetic data fits well with the pseudo-second order kinetic model. Furthermore, the effect of the background electrolytes study indicated that arsenic (V) is specifically sorbed at the surface of these two nanocomposites. This study demonstrated that zirconium intercalated synthetic hectorite as well as zirconium modified natural clays are effective and efficient materials for the selective removal of arsenic (V) from aqueous medium.