• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2001년도 제44회 추계 학술연구 발표회
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• pp.73-73
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• 2001

no Abstract, See Full Text

• Journal of Ginseng Research
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• 제14권2호
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• pp.217-220
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• 1990

Two lignanes, Comp.-I, mp 108-1$0^{\circ}C$ and Comp.-II 50-52$^{\circ}$were isolated from Korea ginseng extract by repeated column chromatographic purification. Comp.-I was identified as gomisin-N and Comp.-II as gomisin-A by spectrometric analysis, both of which have already been described as the anti-hepatotoxic lignan components of Schizandra chinesis Bail.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.32-38
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• 1999

A biosurfactant produced by Tsukamurella sp. 26A was purified by procedures including acid precipitation, ethylacetate extraction, and adsorption chromatography. The purified biosurfactant reduced the surface tension of water from 72 mN/m to 30 mN/m at a concentration of 250 mg/l, whereas the minimum interfacial tension against n-hexadecane was lowered to 1.5 mN/m at a concentration of 40 mg/i. The compound stabilized oil-in-water emulsions with a variety of commercial oils and had strong emulsification and stabilization activities when compared to those of commercial emulsifiers and stabilizers. Surface tension was stable over a broad range of pH (2-12) and temperature ($100^{\circ}C$, 3h). The biosurfactant was identified as glycolipid having a hydrophilic moiety of trehalose.

• 한국미생물·생명공학회지
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• 제8권2호
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• pp.135-142
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• 1980

The process of biological treatment of organic wastewater is principally associated with those of self-purification in the natural water environment. The treatment system has e intensive function of stabilizing wastewater more effectively than in natural water, which is like natural water concentrated in a small space. Biological treatment of wastewater involves activated sludge and various modified process, trickling filter, rotating disk, oxidation ditch, etc. for aerobic decomposition and anaerobic processes such as anaerobic decomposition and methane fermentation. The basic characteristic of these processes is the use of mixed culture for the conversion of pollutants. This review forcuses on the various kinds of microorganisms related to each treatment processes. Kinetic analysis of the activated sludge process is discussed in order to understand the basis of control and maintenance of the biological treatment process.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.861-869
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• 1999

An extracellular protease was purified to homogeneity from the culture supernatant of psychrotrophic bacteria Bacillus sp. JH 108 using procedures including ammonium sulfate fractionation, anion exchange chromatography, gel filtration chromatography, and cation exchange chromatography. The enzyme exhibited a molecular weight of 36 kDa, an optimum pH of 8 to 9, and optimum temperature of $60^{\circ}C$. The enzyme preferentially hydrolyzed leucine at the N-terminus of peptides and thus can be classified as an aminopeptidase. It was strongly inhibited by metal chelating agents such as EDTA and l, l0-phenanthroline. The activity lost by EDTA was restored with $Zn^{2+}{\;}or{\;}Co^{2+}$. These divalent cations also stimulated the native enzyme. This suggests that the enzyme is a metalloprotease acting as a leucine aminopeptidase.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1481-1483
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• 2010

We constructed an efficient T-vector, pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones owing to insertional inactivation of the esterase reporter. Additionally, PCR products were efficiently cloned into this vector without the gel purification steps, owing to the well-designed multi-cloning site that was in-frame fused at the circularly permutated gap of the reporter.

• 생약학회지
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• 제4권4호
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• pp.167-172
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• 1973

Five crystalline substances, which are positive to$L_{IEBERMAN}-B_{URCHARD}$ reaction, were isolated from the unsaponifiable fraction of the fresh leaves of Betula latifolia $K_{OMAROV}$ (Betulaceae)by silica gel column chromatographic purification. Compound $A\;(C_{29}H_{50}O,\;mp\;136^{\circ}), \;B\;(C_{30}H_{52}O_3,\;mp\;165^{\circ}), \;C\;(C_{30}H_{52}O_4,\;mp\;237^{\circ}), \;D\;(C_{30}H_{52}O_3,\;mp\;196^{\circ})\;and\; E\;(C_{30}H_{52}O_4,\;mp\;121^{\circ})$ were isolated. Compound B was characterized as a new tetracyclic triterpenoid. Compounds A, C and D were identified as ${\beta}-sitosterol$, betulatriterpene C, and betulafolienetriol, respectively.

• Journal of Microbiology and Biotechnology
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• 제3권3호
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• pp.188-193
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• 1993

Superoxide dismutase (SOD) was purified from Pseudomonas polycolor to an electrophoretically homogeneous state and partially characterized. SOD was purified by ammonium sulfate fractionation, column chromatography on DEAE-Sephadex A-50, phenyl-Toyopearl 650 M, and gel filtration on Sephadex G-100. The molecular weight and subunit molecular weight of the purified enzyme were estimated to be 40, 000 and 20, 000, respectively. The purified enzyme remained stable at pH 9.0~11.0, $25^{\circ}C$ for 40 hr, but rapidly became inactive below 9.0. SOD was stable up to $45^{\circ}C$ at pH 9.0 with about 80% relative activity, but rapidly became inactive at temperature above that. The enzyme was insensitive to cyanide and fluoride, and sensitive to hydrogen peroxide and azide. The results suggest that the enzyme be an iron-containing SOD.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.60-60
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• 2002

No Abstract, See Full Text

• 한국토양비료학회지
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• 제28권4호
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• pp.319-326
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• 1995

축산폐수정화(畜産廢水淨化)에 흡착성(吸着性)이 높은 천연(天然) zeolite를 이용(利用)할 경우 그 효과(效果)를 검토(檢討)하고 부화처리(浮化處理) 후(後) 정화조(淨化槽)에 남아있는 폐(廢)zeolite를 sudangrass재배(栽培)에 시용(施用)하여 그 생장(生長)에 미치는 영향(影響)을 조사(調査)하여 비료(肥料)로서의 이용가능성(利用可能性)을 검토(檢討)하였다. 폐수처리(廢水處理)에서는 zeolite여상(濾床)을 설치(設置)한 시험구(試驗區)에서 축산폐수(畜産廢水)의 부화효율(浮化效率)은 높았으나, 폭기처리(曝氣處理)를 할 경우 폭기조(曝氣槽)에서는 암모니아태(態) 질소(窒素)의 제거율(除去率)은 높으나 질산화반응(窒酸化反應)에 의해 실산태질소(室酸態窒素)의 농도(濃度)가 유인수(流人水)의 농도(濃度)보다 높았다. Zeolite를 전처리(前處理)에서 이용(利用)한 시험구(試驗區)와 마지막 단계(段階)에서 이용(利用)한 시험구(試驗區)를 비교(比較)한 결과(結果) 전처리(前處理)에서 이용(利用)하는 것이 정화효과(淨化效果)가 높은 것으로 나타났다. 정화처리(淨化處理)에 이용(利用)되었던 zeolite를 수집(收集)하여 sudangrass재배(栽培)에 시용(施用)하여 비효성(肥效性)을 검토(檢討)한 결과(結果) 화학비료(化學肥料) 시비구(施肥區)에 비(比)하여 관행구(慣行區) 폐수흡착(廢水吸着) zeolite를 첨가시용(添加施用)한 처리구(處理區)에서 비효(肥效)가 높은 것으로 판단(判斷)되어 정화처리(淨化處理)에 이용(利用)된 zeolite는 질소성비료(窒素性肥料)의 완효화(緩效化)가 큰 것으로 판단(判斷)된다.